Restriction map for
pF1KA1760
(Linear) (Six-base) MAPSORT of: KIAA1760.seq Check: 5892 from: 1 to: 6630
KIAA1760.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2240 3811 4932 6276 6630
Size: 2240 1571 1121 1344 354
Fragments arranged by size:
2240 1571 1344 1121 354
AatII G_ACGT'C
Cuts at: 0 1094 1532 2729 3062 4255 5276 6630
Size: 1094 438 1197 333 1193 1021 1354
Fragments arranged by size:
1354 1197 1193 1094 1021 438 333
AccI GT'mk_AC
Cuts at: 0 584 5276 6630
Size: 584 4692 1354
Fragments arranged by size:
4692 1354 584
Acc65I G'GTAC_C
Cuts at: 0 6313 6630
Size: 6313 317
AclI AA'CG_TT
Cuts at: 0 3691 6630
Size: 3691 2939
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 838 895 1435 2302 5692 5839 6202
Size: 826 12 57 540 867 3390 147 363
Cuts at: 6202 6403 6424 6630
Size: 201 21 206
Fragments arranged by size:
3390 867 826 540 363 206 201 147
57 21 12
AfeI AGC'GCT
Cuts at: 0 76 257 6630
Size: 76 181 6373
Fragments arranged by size:
6373 181 76
AflIII A'CryG_T
Cuts at: 0 3481 3536 5010 6630
Size: 3481 55 1474 1620
Fragments arranged by size:
3481 1620 1474 55
AhdI GACnn_n'nnGTC
Cuts at: 0 4014 6630
Size: 4014 2616
AleI CACnn'nnGTG
Cuts at: 0 1330 1523 6630
Size: 1330 193 5107
Fragments arranged by size:
5107 1330 193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2122 2336 5761 6630
Size: 2122 214 3425 869
Fragments arranged by size:
3425 2122 869 214
AlwNI CAG_nnn'CTG
Cuts at: 0 168 352 873 1953 2187 3027 3803 4059
Size: 168 184 521 1080 234 840 776 256
Cuts at: 4059 4195 4233 4342 5655 6247 6420 6630
Size: 136 38 109 1313 592 173 210
Fragments arranged by size:
1313 1080 840 776 592 521 256 234
210 184 173 168 136 109 38
ApaI G_GGCC'C
Cuts at: 0 26 71 151 1937 2119 2271 3263 4075
Size: 26 45 80 1786 182 152 992 812
Cuts at: 4075 4520 5398 5521 6630
Size: 445 878 123 1109
Fragments arranged by size:
1786 1109 992 878 812 445 182 152
123 80 45 26
ApaLI G'TGCA_C
Cuts at: 0 1219 6630
Size: 1219 5411
AvaI C'yCGr_G
Cuts at: 0 7 44 304 451 1039 1054 1708 1723
Size: 7 37 260 147 588 15 654 15
Cuts at: 1723 3262 3928 4246 4850 5126 5399 5501 5521
Size: 1539 666 318 604 276 273 102 20
Cuts at: 5521 5620 6485 6630
Size: 99 865 145
Fragments arranged by size:
1539 865 666 654 604 588 318 276
273 260 147 145 102 99 37 20
15 15 7
AvrII C'CTAG_G
Cuts at: 0 6195 6630
Size: 6195 435
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2674 2707 6630
Size: 2674 33 3923
Fragments arranged by size:
3923 2674 33
BamHI G'GATC_C
Cuts at: 0 2058 6630
Size: 2058 4572
BanI G'GyrC_C
Cuts at: 0 141 860 1050 1662 2304 2556 2715 3421
Size: 141 719 190 612 642 252 159 706
Cuts at: 3421 3814 4092 5775 6313 6630
Size: 393 278 1683 538 317
Fragments arranged by size:
1683 719 706 642 612 538 393 317
278 252 190 159 141
BanII G_rGCy'C
Cuts at: 0 8 26 71 151 279 288 311 329
Size: 8 18 45 80 128 9 23 18
Cuts at: 329 452 476 1346 1724 1822 1937 2011 2119
Size: 123 24 870 378 98 115 74 108
Cuts at: 2119 2271 2969 3152 3263 4075 4247 4379 4403
Size: 152 698 183 111 812 172 132 24
Cuts at: 4403 4520 4718 5021 5066 5369 5398 5521 5621
Size: 117 198 303 45 303 29 123 100
Cuts at: 5621 5945 6630
Size: 324 685
Fragments arranged by size:
870 812 698 685 378 324 303 303
198 183 172 152 132 128 123 123
117 115 111 108 100 98 80 74
45 45 29 24 24 23 18 18
9 8
BbeI G_GCGC'C
Cuts at: 0 145 1054 2308 2560 2719 3425 6630
Size: 145 909 1254 252 159 706 3205
Fragments arranged by size:
3205 1254 909 706 252 159 145
BbsI GAAGACnn'nnnn_
Cuts at: 0 814 1406 6011 6630
Size: 814 592 4605 619
Fragments arranged by size:
4605 814 619 592
BbvCI CC'TCA_GC
Cuts at: 0 3796 4662 4668 6630
Size: 3796 866 6 1962
Fragments arranged by size:
3796 1962 866 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2157 2191 3622 3656 6630
Size: 2157 34 1431 34 2974
Fragments arranged by size:
2974 2157 1431 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 3258 3931 4070 4108 4204 5735 6630
Size: 3258 673 139 38 96 1531 895
Fragments arranged by size:
3258 1531 895 673 139 96 38
BglII A'GATC_T
Cuts at: 0 1169 5678 6630
Size: 1169 4509 952
Fragments arranged by size:
4509 1169 952
BlpI GC'TnA_GC
Cuts at: 0 1300 2276 3739 3914 6039 6630
Size: 1300 976 1463 175 2125 591
Fragments arranged by size:
2125 1463 1300 976 591 175
Bme1580I G_kGCm'C
Cuts at: 0 26 71 151 319 1223 1667 1937 2119
Size: 26 45 80 168 904 444 270 182
Cuts at: 2119 2271 2363 3209 3263 3817 4075 4095 4368
Size: 152 92 846 54 554 258 20 273
Cuts at: 4368 4520 4655 4876 5086 5398 5521 6578 6630
Size: 152 135 221 210 312 123 1057 52
Fragments arranged by size:
1057 904 846 554 444 312 273 270
258 221 210 182 168 152 152 135
123 92 80 54 52 45 26 20
BmgBI CAC'GTC
Cuts at: 0 4877 5307 6276 6508 6630
Size: 4877 430 969 232 122
Fragments arranged by size:
4877 969 430 232 122
BmrI ACTGGGnnnn_n'
Cuts at: 0 971 1896 3557 4768 5577 6630
Size: 971 925 1661 1211 809 1053
Fragments arranged by size:
1661 1211 1053 971 925 809
BmtI G_CTAG'C
Cuts at: 0 5105 6630
Size: 5105 1525
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3342 4362 4809 5310 5516 6630
Size: 3342 1020 447 501 206 1114
Fragments arranged by size:
3342 1114 1020 501 447 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1138 1483 2026 2197 2234 2275 2447
Size: 679 459 345 543 171 37 41 172
Cuts at: 2447 2956 2984 3463 3595 4142 4313 4319 4570
Size: 509 28 479 132 547 171 6 251
Cuts at: 4570 4732 5066 5280 5856 5880 6630
Size: 162 334 214 576 24 750
Fragments arranged by size:
750 679 576 547 543 509 479 459
345 334 251 214 172 171 171 162
132 41 37 28 24 6
Bpu10I CC'TnA_GC
Cuts at: 0 446 2963 3068 3146 3796 4060 4397 4662
Size: 446 2517 105 78 650 264 337 265
Cuts at: 4662 4668 5915 5965 6248 6630
Size: 6 1247 50 283 382
Fragments arranged by size:
2517 1247 650 446 382 337 283 265
264 105 78 50 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 526 2309 5842 6630
Size: 490 36 1783 3533 788
Fragments arranged by size:
3533 1783 788 490 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 598 917 4797 4995 5400 6630
Size: 598 319 3880 198 405 1230
Fragments arranged by size:
3880 1230 598 405 319 198
BsaAI yAC'GTr
Cuts at: 0 3647 4650 5011 6630
Size: 3647 1003 361 1619
Fragments arranged by size:
3647 1619 1003 361
BsaHI Gr'CG_yC
Cuts at: 0 142 231 296 904 1051 1091 1474 1529
Size: 142 89 65 608 147 40 383 55
Cuts at: 1529 2305 2557 2716 2726 3059 3422 3755 4252
Size: 776 252 159 10 333 363 333 497
Cuts at: 4252 4358 5273 5428 6630
Size: 106 915 155 1202
Fragments arranged by size:
1202 915 776 608 497 383 363 333
333 252 159 155 147 142 106 89
65 55 40 10
BsaWI w'CCGG_w
Cuts at: 0 641 952 3906 6630
Size: 641 311 2954 2724
Fragments arranged by size:
2954 2724 641 311
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1264 1294 2418 2448 3751 3781 4317 4347
Size: 1264 30 1124 30 1303 30 536 30
Cuts at: 4347 6016 6046 6630
Size: 1669 30 584
Fragments arranged by size:
1669 1303 1264 1124 584 536 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 406 496 499 1081 1366 1741 2141
Size: 127 279 90 3 582 285 375 400
Cuts at: 2141 3331 3838 4107 4650 5331 6630
Size: 1190 507 269 543 681 1299
Fragments arranged by size:
1299 1190 681 582 543 507 400 375
285 279 269 127 90 3
BseYI C'CCAG_C
Cuts at: 0 71 558 1699 1962 2095 2162 2511 2669
Size: 71 487 1141 263 133 67 349 158
Cuts at: 2669 2694 3039 3166 4033 4075 4098 4402 4493
Size: 25 345 127 867 42 23 304 91
Cuts at: 4493 4672 4814 4969 5514 5530 5987 6260 6446
Size: 179 142 155 545 16 457 273 186
Cuts at: 6446 6516 6630
Size: 70 114
Fragments arranged by size:
1141 867 545 487 457 349 345 304
273 263 186 179 158 155 142 133
127 114 91 71 70 67 42 25
23 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 1932 1973 2773 3217 3859 4315 4376
Size: 877 1055 41 800 444 642 456 61
Cuts at: 4376 4991 5437 6001 6256 6316 6630
Size: 615 446 564 255 60 314
Fragments arranged by size:
1055 877 800 642 615 564 456 446
444 314 255 61 60 41
BsiEI CG_ry'CG
Cuts at: 0 64 407 434 534 2423 3762 6630
Size: 64 343 27 100 1889 1339 2868
Fragments arranged by size:
2868 1889 1339 343 100 64 27
BsiHKAI G_wGCw'C
Cuts at: 0 1073 1223 1346 3152 3920 4247 4649 5621
Size: 1073 150 123 1806 768 327 402 972
Cuts at: 5621 6138 6630
Size: 517 492
Fragments arranged by size:
1806 1073 972 768 517 492 402 327
150 123
BsmI GAATG_Cn'
Cuts at: 0 1164 6630
Size: 1164 5466
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1465 4243 4259 4907 6193 6612 6630
Size: 1465 2778 16 648 1286 419 18
Fragments arranged by size:
2778 1465 1286 648 419 18 16
Bsp1286I G_dGCh'C
Cuts at: 0 8 26 71 151 279 288 311 319
Size: 8 18 45 80 128 9 23 8
Cuts at: 319 329 452 476 1073 1223 1346 1667 1724
Size: 10 123 24 597 150 123 321 57
Cuts at: 1724 1822 1937 2011 2119 2271 2363 2969 3152
Size: 98 115 74 108 152 92 606 183
Cuts at: 3152 3209 3263 3817 3920 4075 4095 4247 4368
Size: 57 54 554 103 155 20 152 121
Cuts at: 4368 4379 4403 4520 4649 4655 4718 4876 5021
Size: 11 24 117 129 6 63 158 145
Cuts at: 5021 5066 5086 5369 5398 5521 5621 5945 6138
Size: 45 20 283 29 123 100 324 193
Cuts at: 6138 6578 6630
Size: 440 52
Fragments arranged by size:
606 597 554 440 324 321 283 193
183 158 155 152 152 150 145 129
128 123 123 123 121 117 115 108
103 100 98 92 80 74 63 57
57 54 52 45 45 29 24 24
23 20 20 18 11 10 9 8
8 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1304 1662 2172 2240 2423 3464 3811 4932
Size: 1304 358 510 68 183 1041 347 1121
Cuts at: 4932 4998 5013 6276 6338 6630
Size: 66 15 1263 62 292
Fragments arranged by size:
1304 1263 1121 1041 510 358 347 292
183 68 66 62 15
BsrBI CCG'CTC
Cuts at: 0 663 2275 3067 3684 4739 5969 6630
Size: 663 1612 792 617 1055 1230 661
Fragments arranged by size:
1612 1230 1055 792 663 661 617
BsrFI r'CCGG_y
Cuts at: 0 298 863 1200 1659 2001 2217 2598 2781
Size: 298 565 337 459 342 216 381 183
Cuts at: 2781 2943 3093 3512 5506 6630
Size: 162 150 419 1994 1124
Fragments arranged by size:
1994 1124 565 459 419 381 342 337
298 216 183 162 150
BssHII G'CGCG_C
Cuts at: 0 219 3487 6630
Size: 219 3268 3143
Fragments arranged by size:
3268 3143 219
BssSI C'ACGA_G
Cuts at: 0 1519 4276 4303 6131 6630
Size: 1519 2757 27 1828 499
Fragments arranged by size:
2757 1828 1519 499 27
BstAPI GCAn_nnn'nTGC
Cuts at: 0 168 764 860 1752 1953 2910 3204 5655
Size: 168 596 96 892 201 957 294 2451
Cuts at: 5655 6630
Size: 975
Fragments arranged by size:
2451 975 957 892 596 294 201 168
96
BstEII G'GTnAC_C
Cuts at: 0 1182 1680 3597 6630
Size: 1182 498 1917 3033
Fragments arranged by size:
3033 1917 1182 498
BstXI CCAn_nnnn'nTGG
Cuts at: 0 525 1785 2551 3174 3651 5190 5538 6234
Size: 525 1260 766 623 477 1539 348 696
Cuts at: 6234 6630
Size: 396
Fragments arranged by size:
1539 1260 766 696 623 525 477 396
348
BstYI r'GATC_y
Cuts at: 0 869 1169 1592 2058 5678 6630
Size: 869 300 423 466 3620 952
Fragments arranged by size:
3620 952 869 466 423 300
Bsu36I CC'TnA_GG
Cuts at: 0 795 1384 4226 4781 6630
Size: 795 589 2842 555 1849
Fragments arranged by size:
2842 1849 795 589 555
BtgI C'CryG_G
Cuts at: 0 26 290 311 512 564 1085 1359 1982
Size: 26 264 21 201 52 521 274 623
Cuts at: 1982 2111 2201 2663 3179 3762 6539 6630
Size: 129 90 462 516 583 2777 91
Fragments arranged by size:
2777 623 583 521 516 462 274 264
201 129 91 90 52 26 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 195 327 1362 6630
Size: 195 132 1035 5268
Fragments arranged by size:
5268 1035 195 132
BtsI GCAGTG_nn'
Cuts at: 0 2785 2803 2947 4472 5324 5743 5945 5984
Size: 2785 18 144 1525 852 419 202 39
Cuts at: 5984 6630
Size: 646
Fragments arranged by size:
2785 1525 852 646 419 202 144 39
18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1745 1780 6630
Size: 1745 35 4850
Fragments arranged by size:
4850 1745 35
DraIII CAC_nnn'GTG
Cuts at: 0 2751 6630
Size: 2751 3879
DrdI GACnn_nn'nnGTC
Cuts at: 0 4896 6114 6630
Size: 4896 1218 516
Fragments arranged by size:
4896 1218 516
EaeI y'GGCC_r
Cuts at: 0 61 431 515 531 992 1124 1202 1541
Size: 61 370 84 16 461 132 78 339
Cuts at: 1541 1812 2063 2139 2564 2600 2618 3033 3176
Size: 271 251 76 425 36 18 415 143
Cuts at: 3176 4484 5787 6066 6630
Size: 1308 1303 279 564
Fragments arranged by size:
1308 1303 564 461 425 415 370 339
279 271 251 143 132 84 78 76
61 36 18 16
EagI C'GGCC_G
Cuts at: 0 61 431 531 6630
Size: 61 370 100 6099
Fragments arranged by size:
6099 370 100 61
EarI CTCTTCn'nnn_
Cuts at: 0 473 674 3880 6630
Size: 473 201 3206 2750
Fragments arranged by size:
3206 2750 473 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 52 1646 6071 6630
Size: 52 1594 4425 559
Fragments arranged by size:
4425 1594 559 52
EcoICRI GAG'CTC
Cuts at: 0 1344 3150 4245 5619 6630
Size: 1344 1806 1095 1374 1011
Fragments arranged by size:
1806 1374 1344 1095 1011
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 826 838 895 1138 1435 1483 2026
Size: 679 147 12 57 243 297 48 543
Cuts at: 2026 2197 2234 2275 2302 2447 2956 2984 3463
Size: 171 37 41 27 145 509 28 479
Cuts at: 3463 3595 4142 4313 4319 4570 4732 5066 5280
Size: 132 547 171 6 251 162 334 214
Cuts at: 5280 5692 5839 5856 5880 6202 6403 6424 6630
Size: 412 147 17 24 322 201 21 206
Fragments arranged by size:
679 547 543 509 479 412 334 322
297 251 243 214 206 201 171 171
162 147 147 145 132 57 48 41
37 28 27 24 21 17 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1465 2179 4063 4350 5284 6251 6488 6630
Size: 1465 714 1884 287 934 967 237 142
Fragments arranged by size:
1884 1465 967 934 714 287 237 142
EcoO109I rG'GnC_Cy
Cuts at: 0 22 67 68 879 1933 1967 2184 2267
Size: 22 45 1 811 1054 34 217 83
Cuts at: 2267 2268 3072 3933 4072 4281 4418 4516 4676
Size: 1 804 861 139 209 137 98 160
Cuts at: 4676 4758 5138 5289 5394 5395 5518 5550 5905
Size: 82 380 151 105 1 123 32 355
Cuts at: 5905 5919 6218 6244 6256 6630
Size: 14 299 26 12 374
Fragments arranged by size:
1054 861 811 804 380 374 355 299
217 209 160 151 139 137 123 105
98 83 82 45 34 32 26 22
14 12 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3630 6333 6630
Size: 3630 2703 297
Fragments arranged by size:
3630 2703 297
HaeII r_GCGC'y
Cuts at: 0 78 145 177 259 752 1054 1778 1891
Size: 78 67 32 82 493 302 724 113
Cuts at: 1891 2308 2560 2719 3425 4000 5126 6523 6630
Size: 417 252 159 706 575 1126 1397 107
Fragments arranged by size:
1397 1126 724 706 575 493 417 302
252 159 113 107 82 78 67 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1020 1052 2418 2450 3751 3783 4000 4032
Size: 1020 32 1366 32 1301 32 217 32
Cuts at: 4032 4171 4203 5158 5190 5356 5388 5703 5735
Size: 139 32 955 32 166 32 315 32
Cuts at: 5735 6016 6048 6468 6492 6500 6524 6630
Size: 281 32 420 24 8 24 106
Fragments arranged by size:
1366 1301 1020 955 420 315 281 217
166 139 106 32 32 32 32 32
32 32 32 32 24 24 8
KasI G'GCGC_C
Cuts at: 0 141 1050 2304 2556 2715 3421 6630
Size: 141 909 1254 252 159 706 3209
Fragments arranged by size:
3209 1254 909 706 252 159 141
KpnI G_GTAC'C
Cuts at: 0 6317 6630
Size: 6317 313
MluI A'CGCG_T
Cuts at: 0 3481 6630
Size: 3481 3149
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 3794 4535 6630
Size: 83 3711 741 2095
Fragments arranged by size:
3711 2095 741 83
MscI TGG'CCA
Cuts at: 0 517 994 1126 1543 2566 3035 3178 4486
Size: 517 477 132 417 1023 469 143 1308
Cuts at: 4486 6068 6630
Size: 1582 562
Fragments arranged by size:
1582 1308 1023 562 517 477 469 417
143 132
MslI CAynn'nnrTG
Cuts at: 0 1076 1330 1523 2104 3649 6630
Size: 1076 254 193 581 1545 2981
Fragments arranged by size:
2981 1545 1076 581 254 193
MspA1I CmG'CkG
Cuts at: 0 28 292 352 566 669 857 1811 2157
Size: 28 264 60 214 103 188 954 346
Cuts at: 2157 2280 2698 3004 3249 3294 3413 3573 4014
Size: 123 418 306 245 45 119 160 441
Cuts at: 4014 4415 4672 4809 6381 6402 6630
Size: 401 257 137 1572 21 228
Fragments arranged by size:
1572 954 441 418 401 346 306 264
257 245 228 214 188 160 137 123
119 103 60 45 28 21
NaeI GCC'GGC
Cuts at: 0 300 865 1202 2783 2945 3514 5508 6630
Size: 300 565 337 1581 162 569 1994 1122
Fragments arranged by size:
1994 1581 1122 569 565 337 300 162
NarI GG'CG_CC
Cuts at: 0 142 1051 2305 2557 2716 3422 6630
Size: 142 909 1254 252 159 706 3208
Fragments arranged by size:
3208 1254 909 706 252 159 142
NgoMIV G'CCGG_C
Cuts at: 0 298 863 1200 2781 2943 3512 5506 6630
Size: 298 565 337 1581 162 569 1994 1124
Fragments arranged by size:
1994 1581 1124 569 565 337 298 162
NheI G'CTAG_C
Cuts at: 0 5101 6630
Size: 5101 1529
NspI r_CATG'y
Cuts at: 0 1116 3738 6630
Size: 1116 2622 2892
Fragments arranged by size:
2892 2622 1116
PasI CC'CwG_GG
Cuts at: 0 386 2551 3775 4512 4577 6630
Size: 386 2165 1224 737 65 2053
Fragments arranged by size:
2165 2053 1224 737 386 65
PflMI CCAn_nnn'nTGG
Cuts at: 0 3594 4821 6630
Size: 3594 1227 1809
Fragments arranged by size:
3594 1809 1227
PfoI T'CCnGG_A
Cuts at: 0 2054 6549 6630
Size: 2054 4495 81
Fragments arranged by size:
4495 2054 81
PmlI CAC'GTG
Cuts at: 0 4650 5011 6630
Size: 4650 361 1619
Fragments arranged by size:
4650 1619 361
PpuMI rG'GwC_Cy
Cuts at: 0 2184 3072 4418 4676 4758 5138 5550 5919
Size: 2184 888 1346 258 82 380 412 369
Cuts at: 5919 6218 6630
Size: 299 412
Fragments arranged by size:
2184 1346 888 412 412 380 369 299
258 82
PspOMI G'GGCC_C
Cuts at: 0 22 67 147 1933 2115 2267 3259 4071
Size: 22 45 80 1786 182 152 992 812
Cuts at: 4071 4516 5394 5517 6630
Size: 445 878 123 1113
Fragments arranged by size:
1786 1113 992 878 812 445 182 152
123 80 45 22
PspXI vC'TCGA_Gb
Cuts at: 0 4850 5126 5620 6485 6630
Size: 4850 276 494 865 145
Fragments arranged by size:
4850 865 494 276 145
PstI C_TGCA'G
Cuts at: 0 173 638 707 1610 1673 2315 2528 3677
Size: 173 465 69 903 63 642 213 1149
Cuts at: 3677 5492 5651 5693 5952 6630
Size: 1815 159 42 259 678
Fragments arranged by size:
1815 1149 903 678 642 465 259 213
173 159 69 63 42
PvuII CAG'CTG
Cuts at: 0 857 2280 3004 3294 3413 3573 4014 4672
Size: 857 1423 724 290 119 160 441 658
Cuts at: 4672 4809 6381 6402 6630
Size: 137 1572 21 228
Fragments arranged by size:
1572 1423 857 724 658 441 290 228
160 137 119 21
SacI G_AGCT'C
Cuts at: 0 1346 3152 4247 5621 6630
Size: 1346 1806 1095 1374 1009
Fragments arranged by size:
1806 1374 1346 1095 1009
SacII CC_GC'GG
Cuts at: 0 29 293 567 6630
Size: 29 264 274 6063
Fragments arranged by size:
6063 274 264 29
SanDI GG'GwC_CC
Cuts at: 0 4418 4676 5138 6630
Size: 4418 258 462 1492
Fragments arranged by size:
4418 1492 462 258
SbfI CC_TGCA'GG
Cuts at: 0 1673 3677 6630
Size: 1673 2004 2953
Fragments arranged by size:
2953 2004 1673
SexAI A'CCwGG_T
Cuts at: 0 6582 6630
Size: 6582 48
SfcI C'TryA_G
Cuts at: 0 169 634 703 1606 1669 2311 2524 3673
Size: 169 465 69 903 63 642 213 1149
Cuts at: 3673 5488 5647 5689 5948 6630
Size: 1815 159 42 259 682
Fragments arranged by size:
1815 1149 903 682 642 465 259 213
169 159 69 63 42
SfoI GGC'GCC
Cuts at: 0 143 1052 2306 2558 2717 3423 6630
Size: 143 909 1254 252 159 706 3207
Fragments arranged by size:
3207 1254 909 706 252 159 143
SgrAI Cr'CCGG_yG
Cuts at: 0 1659 6630
Size: 1659 4971
SmaI CCC'GGG
Cuts at: 0 9 453 1710 3264 6630
Size: 9 444 1257 1554 3366
Fragments arranged by size:
3366 1554 1257 444 9
SmlI C'TyrA_G
Cuts at: 0 505 541 2324 4246 4850 5126 5620 5857
Size: 505 36 1783 1922 604 276 494 237
Cuts at: 5857 6485 6630
Size: 628 145
Fragments arranged by size:
1922 1783 628 604 505 494 276 237
145 36
SphI G_CATG'C
Cuts at: 0 1116 6630
Size: 1116 5514
SrfI GCCC'GGGC
Cuts at: 0 9 453 1710 6630
Size: 9 444 1257 4920
Fragments arranged by size:
4920 1257 444 9
SspI AAT'ATT
Cuts at: 0 942 6630
Size: 942 5688
StuI AGG'CCT
Cuts at: 0 701 3325 5496 6630
Size: 701 2624 2171 1134
Fragments arranged by size:
2624 2171 1134 701
StyI C'CwwG_G
Cuts at: 0 752 842 1427 1613 4369 4433 4820 6069
Size: 752 90 585 186 2756 64 387 1249
Cuts at: 6069 6195 6630
Size: 126 435
Fragments arranged by size:
2756 1249 752 585 435 387 186 126
90 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 600 924 1383 3053 3226 5544 6104 6630
Size: 600 324 459 1670 173 2318 560 526
Fragments arranged by size:
2318 1670 600 560 526 459 324 173
TatI w'GTAC_w
Cuts at: 0 1829 6630
Size: 1829 4801
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 899 931 3460 3492 4317 4349 4928 4960
Size: 899 32 2529 32 825 32 579 32
Cuts at: 4960 6531 6563 6630
Size: 1571 32 67
Fragments arranged by size:
2529 1571 899 825 579 67 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 6567 6630
Size: 6567 63
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1696 2373 3028 3651 4608 6231 6630
Size: 1696 677 655 623 957 1623 399
Fragments arranged by size:
1696 1623 957 677 655 623 399
XhoI C'TCGA_G
Cuts at: 0 4246 4850 5126 5620 6485 6630
Size: 4246 604 276 494 865 145
Fragments arranged by size:
4246 865 604 494 276 145
XmaI C'CCGG_G
Cuts at: 0 7 451 1708 3262 6630
Size: 7 444 1257 1554 3368
Fragments arranged by size:
3368 1554 1257 444 7
XmnI GAAnn'nnTTC
Cuts at: 0 823 6630
Size: 823 5807
ZraI GAC'GTC
Cuts at: 0 1092 1530 2727 3060 4253 5274 6630
Size: 1092 438 1197 333 1193 1021 1356
Fragments arranged by size:
1356 1197 1193 1092 1021 438 333
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII
SacI SacII SanDI SbfI SexAI SfcI SfoI SgrAI
SmaI SmlI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AloI ApoI AscI AseI AsiSI BciVI
BclI BfrBI BsaBI BsiWI BspEI BspHI BsrDI BsrGI
BstBI BstZ17I ClaI DraI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI MfeI NcoI NdeI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PsrI PvuI RsrII SalI SapI ScaI SfiI SgfI
SnaBI SpeI SwaI XbaI