Restriction map for
pF1KA1762
(Linear) (Six-base) MAPSORT of: KIAA1762.seq Check: 8722 from: 1 to: 4566
KIAA1762.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2748 3471 3483 4397 4566
Size: 2748 723 12 914 169
Fragments arranged by size:
2748 914 723 169 12
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1565 2141 3100 3415 4566
Size: 281 1284 576 959 315 1151
Fragments arranged by size:
1284 1151 959 576 315 281
AflII C'TTAA_G
Cuts at: 0 916 4566
Size: 916 3650
AflIII A'CryG_T
Cuts at: 0 1350 4566
Size: 1350 3216
AleI CACnn'nnGTG
Cuts at: 0 3646 4566
Size: 3646 920
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2261 4566
Size: 2261 2305
AlwNI CAG_nnn'CTG
Cuts at: 0 241 936 1519 2041 3238 3360 3433 3885
Size: 241 695 583 522 1197 122 73 452
Cuts at: 3885 4566
Size: 681
Fragments arranged by size:
1197 695 681 583 522 452 241 122
73
ApaI G_GGCC'C
Cuts at: 0 781 977 1498 2050 2580 2780 2878 4438
Size: 781 196 521 552 530 200 98 1560
Cuts at: 4438 4566
Size: 128
Fragments arranged by size:
1560 781 552 530 521 200 196 128
98
ApaLI G'TGCA_C
Cuts at: 0 426 597 4566
Size: 426 171 3969
Fragments arranged by size:
3969 426 171
AvaI C'yCGr_G
Cuts at: 0 677 1270 2579 3490 4566
Size: 677 593 1309 911 1076
Fragments arranged by size:
1309 1076 911 677 593
BanI G'GyrC_C
Cuts at: 0 553 900 1620 2619 3519 3724 4317 4566
Size: 553 347 720 999 900 205 593 249
Fragments arranged by size:
999 900 720 593 553 347 249 205
BanII G_rGCy'C
Cuts at: 0 320 631 734 781 977 1498 2050 2411
Size: 320 311 103 47 196 521 552 361
Cuts at: 2411 2537 2580 2780 2878 3390 3761 4079 4288
Size: 126 43 200 98 512 371 318 209
Cuts at: 4288 4297 4438 4566
Size: 9 141 128
Fragments arranged by size:
552 521 512 371 361 320 318 311
209 200 196 141 128 126 103 98
47 43 9
BbeI G_GCGC'C
Cuts at: 0 3523 4566
Size: 3523 1043
BbsI GAAGACnn'nnnn_
Cuts at: 0 269 3856 4566
Size: 269 3587 710
Fragments arranged by size:
3587 710 269
BbvCI CC'TCA_GC
Cuts at: 0 290 4566
Size: 290 4276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 652 686 2490 2524 4566
Size: 652 34 1804 34 2042
Fragments arranged by size:
2042 1804 652 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1297 3614 4566
Size: 1297 2317 952
Fragments arranged by size:
2317 1297 952
BglII A'GATC_T
Cuts at: 0 2265 2934 4566
Size: 2265 669 1632
Fragments arranged by size:
2265 1632 669
BlpI GC'TnA_GC
Cuts at: 0 1058 1067 2080 3551 4566
Size: 1058 9 1013 1471 1015
Fragments arranged by size:
1471 1058 1015 1013 9
Bme1580I G_kGCm'C
Cuts at: 0 430 558 601 766 781 905 977 1238
Size: 430 128 43 165 15 124 72 261
Cuts at: 1238 1498 1542 2050 2580 2780 2878 4322 4438
Size: 260 44 508 530 200 98 1444 116
Cuts at: 4438 4566
Size: 128
Fragments arranged by size:
1444 530 508 430 261 260 200 165
128 128 124 116 98 72 44 43
15
BmrI ACTGGGnnnn_n'
Cuts at: 0 320 1856 2237 2720 2855 3029 3257 4566
Size: 320 1536 381 483 135 174 228 1309
Fragments arranged by size:
1536 1309 483 381 320 228 174 135
BmtI G_CTAG'C
Cuts at: 0 1757 4566
Size: 1757 2809
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 339 3358 4566
Size: 339 3019 1208
Fragments arranged by size:
3019 1208 339
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 218 323 658 907 1581 1626 2015
Size: 206 12 105 335 249 674 45 389
Cuts at: 2015 2048 2169 2885 3008 3029 3762 4036 4103
Size: 33 121 716 123 21 733 274 67
Cuts at: 4103 4566
Size: 463
Fragments arranged by size:
733 716 674 463 389 335 274 249
206 123 121 105 67 45 33 21
12
Bpu10I CC'TnA_GC
Cuts at: 0 155 290 1384 2447 4073 4566
Size: 155 135 1094 1063 1626 493
Fragments arranged by size:
1626 1094 1063 493 155 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1318 1900 3355 3436 3814 4412 4566
Size: 949 369 582 1455 81 378 598 154
Fragments arranged by size:
1455 949 598 582 378 369 154 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 92 1472 3681 3780 4566
Size: 92 1380 2209 99 786
Fragments arranged by size:
2209 1380 786 99 92
BsaHI Gr'CG_yC
Cuts at: 0 3520 4566
Size: 3520 1046
BsaWI w'CCGG_w
Cuts at: 0 1834 4566
Size: 1834 2732
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 613 643 1017 1047 2503 2533 3760 3790
Size: 613 30 374 30 1456 30 1227 30
Cuts at: 3790 4077 4107 4378 4408 4507 4537 4566
Size: 287 30 271 30 99 30 29
Fragments arranged by size:
1456 1227 613 374 287 271 99 30
30 30 30 30 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 263 307 1022 1025 1853 1963 1966 1993
Size: 263 44 715 3 828 110 3 27
Cuts at: 1993 2023 2089 2408 2539 3163 3591 3601 3804
Size: 30 66 319 131 624 428 10 203
Cuts at: 3804 4039 4386 4566
Size: 235 347 180
Fragments arranged by size:
828 715 624 428 347 319 263 235
203 180 131 110 66 44 30 27
10 3 3
BseYI C'CCAG_C
Cuts at: 0 855 991 2148 2780 3141 3238 3278 3389
Size: 855 136 1157 632 361 97 40 111
Cuts at: 3389 3426 3444 3540 3561 3861 3907 3941 4064
Size: 37 18 96 21 300 46 34 123
Cuts at: 4064 4566
Size: 502
Fragments arranged by size:
1157 855 632 502 361 300 136 123
111 97 96 46 40 37 34 21
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 521 829 983 4311 4566
Size: 11 510 308 154 3328 255
Fragments arranged by size:
3328 510 308 255 154 11
BsiEI CG_ry'CG
Cuts at: 0 1632 4566
Size: 1632 2934
BsiHKAI G_wGCw'C
Cuts at: 0 320 430 601 631 2250 3247 4566
Size: 320 110 171 30 1619 997 1319
Fragments arranged by size:
1619 1319 997 320 171 110 30
BsmI GAATG_Cn'
Cuts at: 0 1788 3198 4566
Size: 1788 1410 1368
Fragments arranged by size:
1788 1410 1368
Bsp1286I G_dGCh'C
Cuts at: 0 320 430 558 601 631 734 766 781
Size: 320 110 128 43 30 103 32 15
Cuts at: 781 905 977 1238 1498 1542 2050 2250 2411
Size: 124 72 261 260 44 508 200 161
Cuts at: 2411 2537 2580 2780 2878 3247 3390 3761 4079
Size: 126 43 200 98 369 143 371 318
Cuts at: 4079 4288 4297 4322 4438 4566
Size: 209 9 25 116 128
Fragments arranged by size:
508 371 369 320 318 261 260 209
200 200 161 143 128 128 126 124
116 110 103 98 72 44 43 43
32 30 25 15 9
BspHI T'CATG_A
Cuts at: 0 3086 4566
Size: 3086 1480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 851 2748 3471 3483 4397 4566
Size: 851 1897 723 12 914 169
Fragments arranged by size:
1897 914 851 723 169 12
BsrBI CCG'CTC
Cuts at: 0 323 1071 1083 4566
Size: 323 748 12 3483
Fragments arranged by size:
3483 748 323 12
BsrDI GCAATG_nn'
Cuts at: 0 1138 3895 4309 4566
Size: 1138 2757 414 257
Fragments arranged by size:
2757 1138 414 257
BsrFI r'CCGG_y
Cuts at: 0 550 1627 1634 3522 4566
Size: 550 1077 7 1888 1044
Fragments arranged by size:
1888 1077 1044 550 7
BssSI C'ACGA_G
Cuts at: 0 1342 3197 4566
Size: 1342 1855 1369
Fragments arranged by size:
1855 1369 1342
BstAPI GCAn_nnn'nTGC
Cuts at: 0 605 936 1233 1871 3290 4566
Size: 605 331 297 638 1419 1276
Fragments arranged by size:
1419 1276 638 605 331 297
BstBI TT'CG_AA
Cuts at: 0 1407 2601 4566
Size: 1407 1194 1965
Fragments arranged by size:
1965 1407 1194
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3648 3661 4566
Size: 3648 13 905
Fragments arranged by size:
3648 905 13
BstYI r'GATC_y
Cuts at: 0 144 641 2265 2459 2934 4261 4566
Size: 144 497 1624 194 475 1327 305
Fragments arranged by size:
1624 1327 497 475 305 194 144
Bsu36I CC'TnA_GG
Cuts at: 0 195 1262 1520 2042 3434 3603 4566
Size: 195 1067 258 522 1392 169 963
Fragments arranged by size:
1392 1067 963 522 258 195 169
BtgI C'CryG_G
Cuts at: 0 284 1091 4181 4566
Size: 284 807 3090 385
Fragments arranged by size:
3090 807 385 284
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4180 4566
Size: 4180 386
BtsI GCAGTG_nn'
Cuts at: 0 569 910 1160 2385 2639 2756 3264 4335
Size: 569 341 250 1225 254 117 508 1071
Cuts at: 4335 4463 4566
Size: 128 103
Fragments arranged by size:
1225 1071 569 508 341 254 250 128
117 103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 366 401 818 853 4292 4327 4566
Size: 366 35 417 35 3439 35 239
Fragments arranged by size:
3439 417 366 239 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 434 2863 4566
Size: 434 2429 1703
Fragments arranged by size:
2429 1703 434
DrdI GACnn_nn'nnGTC
Cuts at: 0 1736 4566
Size: 1736 2830
EaeI y'GGCC_r
Cuts at: 0 54 207 1094 1629 3362 4566
Size: 54 153 887 535 1733 1204
Fragments arranged by size:
1733 1204 887 535 153 54
EagI C'GGCC_G
Cuts at: 0 1629 4566
Size: 1629 2937
EarI CTCTTCn'nnn_
Cuts at: 0 155 293 722 871 1318 1541 1577 1940
Size: 155 138 429 149 447 223 36 363
Cuts at: 1940 1952 1970 1979 2117 2864 3391 4566
Size: 12 18 9 138 747 527 1175
Fragments arranged by size:
1175 747 527 447 429 363 223 155
149 138 138 36 18 12 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 266 4566
Size: 266 4300
EcoICRI GAG'CTC
Cuts at: 0 318 629 4566
Size: 318 311 3937
Fragments arranged by size:
3937 318 311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 218 281 323 658 907 1565 1581
Size: 206 12 63 42 335 249 658 16
Cuts at: 1581 1626 2015 2048 2141 2169 2885 3008 3029
Size: 45 389 33 93 28 716 123 21
Cuts at: 3029 3100 3415 3762 4036 4103 4566
Size: 71 315 347 274 67 463
Fragments arranged by size:
716 658 463 389 347 335 315 274
249 206 123 93 71 67 63 45
42 33 28 21 16 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1523 1945 2033 3740 4118 4566
Size: 1523 422 88 1707 378 448
Fragments arranged by size:
1707 1523 448 422 378 88
EcoO109I rG'GnC_Cy
Cuts at: 0 115 180 777 778 973 1117 1266 1298
Size: 115 65 597 1 195 144 149 32
Cuts at: 1298 1532 2019 2038 2046 2193 2576 2777 2874
Size: 234 487 19 8 147 383 201 97
Cuts at: 2874 3004 3056 3264 3456 3745 3802 3953 4239
Size: 130 52 208 192 289 57 151 286
Cuts at: 4239 4434 4566
Size: 195 132
Fragments arranged by size:
597 487 383 289 286 234 208 201
195 195 192 151 149 147 144 132
130 115 97 65 57 52 32 19
8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 462 4566
Size: 462 4104
FspI TGC'GCA
Cuts at: 0 2431 4566
Size: 2431 2135
HaeII r_GCGC'y
Cuts at: 0 1703 2427 3051 3523 4566
Size: 1703 724 624 472 1043
Fragments arranged by size:
1703 1043 724 624 472
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 660 692 1807 1839 2503 2535
Size: 178 32 450 32 1115 32 664 32
Cuts at: 2535 3760 3792 4566
Size: 1225 32 774
Fragments arranged by size:
1225 1115 774 664 450 178 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 2894 3104 4566
Size: 2894 210 1462
Fragments arranged by size:
2894 1462 210
KasI G'GCGC_C
Cuts at: 0 3519 4566
Size: 3519 1047
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2513 3987 4550 4566
Size: 2513 1474 563 16
Fragments arranged by size:
2513 1474 563 16
MscI TGG'CCA
Cuts at: 0 56 209 1096 3364 4566
Size: 56 153 887 2268 1202
Fragments arranged by size:
2268 1202 887 153 56
MslI CAynn'nnrTG
Cuts at: 0 2159 3646 4566
Size: 2159 1487 920
Fragments arranged by size:
2159 1487 920
MspA1I CmG'CkG
Cuts at: 0 343 936 1021 3045 3078 3238 3393 3561
Size: 343 593 85 2024 33 160 155 168
Cuts at: 3561 3928 4566
Size: 367 638
Fragments arranged by size:
2024 638 593 367 343 168 160 155
85 33
NaeI GCC'GGC
Cuts at: 0 1629 4566
Size: 1629 2937
NarI GG'CG_CC
Cuts at: 0 3520 4566
Size: 3520 1046
NcoI C'CATG_G
Cuts at: 0 284 1091 4566
Size: 284 807 3475
Fragments arranged by size:
3475 807 284
NgoMIV G'CCGG_C
Cuts at: 0 1627 4566
Size: 1627 2939
NheI G'CTAG_C
Cuts at: 0 1753 4566
Size: 1753 2813
NspI r_CATG'y
Cuts at: 0 651 1354 2481 4349 4566
Size: 651 703 1127 1868 217
Fragments arranged by size:
1868 1127 703 651 217
PasI CC'CwG_GG
Cuts at: 0 2414 2611 2980 4566
Size: 2414 197 369 1586
Fragments arranged by size:
2414 1586 369 197
PciI A'CATG_T
Cuts at: 0 1350 4566
Size: 1350 3216
PflMI CCAn_nnn'nTGG
Cuts at: 0 4341 4566
Size: 4341 225
PpuMI rG'GwC_Cy
Cuts at: 0 180 1532 2193 3004 3056 4566
Size: 180 1352 661 811 52 1510
Fragments arranged by size:
1510 1352 811 661 180 52
PshAI GACnn'nnGTC
Cuts at: 0 3856 4566
Size: 3856 710
PsiI TTA'TAA
Cuts at: 0 479 4566
Size: 479 4087
PspOMI G'GGCC_C
Cuts at: 0 777 973 1494 2046 2576 2776 2874 4434
Size: 777 196 521 552 530 200 98 1560
Cuts at: 4434 4566
Size: 132
Fragments arranged by size:
1560 777 552 530 521 200 196 132
98
PstI C_TGCA'G
Cuts at: 0 330 610 1167 1682 1867 2240 2931 3077
Size: 330 280 557 515 185 373 691 146
Cuts at: 3077 4566
Size: 1489
Fragments arranged by size:
1489 691 557 515 373 330 280 185
146
PvuII CAG'CTG
Cuts at: 0 343 936 1021 3078 3393 3928 4566
Size: 343 593 85 2057 315 535 638
Fragments arranged by size:
2057 638 593 535 343 315 85
SacI G_AGCT'C
Cuts at: 0 320 631 4566
Size: 320 311 3935
Fragments arranged by size:
3935 320 311
SanDI GG'GwC_CC
Cuts at: 0 1532 4566
Size: 1532 3034
SapI GCTCTTCn'nnn_
Cuts at: 0 722 1541 4566
Size: 722 819 3025
Fragments arranged by size:
3025 819 722
SfcI C'TryA_G
Cuts at: 0 326 606 1163 1678 1863 2236 2927 3051
Size: 326 280 557 515 185 373 691 124
Cuts at: 3051 3073 3223 3946 4566
Size: 22 150 723 620
Fragments arranged by size:
723 691 620 557 515 373 326 280
185 150 124 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1297 3614 4566
Size: 1297 2317 952
Fragments arranged by size:
2317 1297 952
SfoI GGC'GCC
Cuts at: 0 3521 4566
Size: 3521 1045
SmaI CCC'GGG
Cuts at: 0 2581 4566
Size: 2581 1985
SmlI C'TyrA_G
Cuts at: 0 916 964 1333 1915 3370 3451 3829 4427
Size: 916 48 369 582 1455 81 378 598
Cuts at: 4427 4566
Size: 139
Fragments arranged by size:
1455 916 598 582 378 369 139 81
48
SspI AAT'ATT
Cuts at: 0 462 4566
Size: 462 4104
StuI AGG'CCT
Cuts at: 0 3608 4566
Size: 3608 958
StyI C'CwwG_G
Cuts at: 0 284 734 836 1052 1091 3203 3761 4566
Size: 284 450 102 216 39 2112 558 805
Fragments arranged by size:
2112 805 558 450 284 216 102 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 45 466 576 2874 3618 4455 4566
Size: 45 421 110 2298 744 837 111
Fragments arranged by size:
2298 837 744 421 111 110 45
TatI w'GTAC_w
Cuts at: 0 1211 4566
Size: 1211 3355
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1017 1049 4077 4109 4246 4278 4378 4410
Size: 1017 32 3028 32 137 32 100 32
Cuts at: 4410 4507 4539 4566
Size: 97 32 27
Fragments arranged by size:
3028 1017 137 100 97 32 32 32
32 32 27
Tth111I GACn'n_nGTC
Cuts at: 0 3736 3772 4566
Size: 3736 36 794
Fragments arranged by size:
3736 794 36
XbaI T'CTAG_A
Cuts at: 0 1179 4566
Size: 1179 3387
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 387 1059 3970 4566
Size: 387 672 2911 596
Fragments arranged by size:
2911 672 596 387
XmaI C'CCGG_G
Cuts at: 0 2579 4566
Size: 2579 1987
XmnI GAAnn'nnTTC
Cuts at: 0 1312 4566
Size: 1312 3254
Enzymes that do cut:
AarI AcuI AflII AflIII AleI AlfI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PpuMI PshAI PsiI PspOMI PstI PvuII SacI
SanDI SapI SfcI SfiI SfoI SmaI SmlI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AloI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BfrBI BmgBI BsaAI BsaBI BsiWI BsmBI BspEI
BsrGI BssHII BstEII BstZ17I ClaI DraI EcoRI EcoRV
FseI FspAI HincII HpaI KpnI MfeI MluI NdeI
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI
SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
XhoI ZraI