Restriction map for
pF1KA1763
(Linear) (Six-base) MAPSORT of: KIAA1763.seq Check: 5329 from: 1 to: 3924
KIAA1763.orf
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1321 3466 3924
Size: 1321 2145 458
Fragments arranged by size:
2145 1321 458
AccI GT'mk_AC
Cuts at: 0 3845 3924
Size: 3845 79
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 1219 1599 1609 1793 1981 3556 3924
Size: 114 1105 380 10 184 188 1575 368
Fragments arranged by size:
1575 1105 380 368 188 184 114 10
AflII C'TTAA_G
Cuts at: 0 1282 3924
Size: 1282 2642
AflIII A'CryG_T
Cuts at: 0 3585 3924
Size: 3585 339
AleI CACnn'nnGTG
Cuts at: 0 277 1394 3924
Size: 277 1117 2530
Fragments arranged by size:
2530 1117 277
AlwNI CAG_nnn'CTG
Cuts at: 0 386 952 3568 3924
Size: 386 566 2616 356
Fragments arranged by size:
2616 566 386 356
ApoI r'AATT_y
Cuts at: 0 78 631 898 981 2376 3127 3400 3924
Size: 78 553 267 83 1395 751 273 524
Fragments arranged by size:
1395 751 553 524 273 267 83 78
AseI AT'TA_AT
Cuts at: 0 734 3924
Size: 734 3190
BamHI G'GATC_C
Cuts at: 0 1504 3924
Size: 1504 2420
BanI G'GyrC_C
Cuts at: 0 1465 2627 3924
Size: 1465 1162 1297
Fragments arranged by size:
1465 1297 1162
BanII G_rGCy'C
Cuts at: 0 149 607 1129 2844 3178 3692 3924
Size: 149 458 522 1715 334 514 232
Fragments arranged by size:
1715 522 514 458 334 232 149
BbvCI CC'TCA_GC
Cuts at: 0 3854 3924
Size: 3854 70
BciVI GTATCCnnnnn_n'
Cuts at: 0 1264 3924
Size: 1264 2660
BclI T'GATC_A
Cuts at: 0 588 804 3122 3203 3924
Size: 588 216 2318 81 721
Fragments arranged by size:
2318 721 588 216 81
BglII A'GATC_T
Cuts at: 0 1578 3924
Size: 1578 2346
BlpI GC'TnA_GC
Cuts at: 0 3109 3517 3924
Size: 3109 408 407
Fragments arranged by size:
3109 408 407
BmrI ACTGGGnnnn_n'
Cuts at: 0 321 1310 1689 2142 2347 3924
Size: 321 989 379 453 205 1577
Fragments arranged by size:
1577 989 453 379 321 205
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 40 736 2550 3924
Size: 40 696 1814 1374
Fragments arranged by size:
1814 1374 696 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 421 1113 1181 1722 2837 3054 3924
Size: 135 286 692 68 541 1115 217 870
Fragments arranged by size:
1115 870 692 541 286 217 135 68
Bpu10I CC'TnA_GC
Cuts at: 0 601 1445 3854 3924
Size: 601 844 2409 70
Fragments arranged by size:
2409 844 601 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 272 1413 3924
Size: 10 262 1141 2511
Fragments arranged by size:
2511 1141 262 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 215 2804 3924
Size: 215 2589 1120
Fragments arranged by size:
2589 1120 215
BsaAI yAC'GTr
Cuts at: 0 3525 3588 3924
Size: 3525 63 336
Fragments arranged by size:
3525 336 63
BsaBI GATnn'nnATC
Cuts at: 0 3754 3924
Size: 3754 170
BsaWI w'CCGG_w
Cuts at: 0 2225 3924
Size: 2225 1699
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3058 3088 3924
Size: 3058 30 836
Fragments arranged by size:
3058 836 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1180 1183 1249 1421 1757 3924
Size: 1180 3 66 172 336 2167
Fragments arranged by size:
2167 1180 336 172 66 3
BseYI C'CCAG_C
Cuts at: 0 73 1437 1960 3553 3611 3924
Size: 73 1364 523 1593 58 313
Fragments arranged by size:
1593 1364 523 313 73 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2036 2614 2881 2940 3428 3452 3532 3924
Size: 2036 578 267 59 488 24 80 392
Fragments arranged by size:
2036 578 488 392 267 80 59 24
BsiHKAI G_wGCw'C
Cuts at: 0 149 827 1129 1708 2024 3178 3924
Size: 149 678 302 579 316 1154 746
Fragments arranged by size:
1154 746 678 579 316 302 149
BsmI GAATG_Cn'
Cuts at: 0 512 816 1359 2316 2792 3924
Size: 512 304 543 957 476 1132
Fragments arranged by size:
1132 957 543 512 476 304
Bsp1286I G_dGCh'C
Cuts at: 0 149 607 827 1129 1708 2024 2844 3178
Size: 149 458 220 302 579 316 820 334
Cuts at: 3178 3692 3924
Size: 514 232
Fragments arranged by size:
820 579 514 458 334 316 302 232
220 149
BspHI T'CATG_A
Cuts at: 0 908 3924
Size: 908 3016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1321 3466 3924
Size: 1321 2145 458
Fragments arranged by size:
2145 1321 458
BsrDI GCAATG_nn'
Cuts at: 0 1061 2880 2916 3206 3924
Size: 1061 1819 36 290 718
Fragments arranged by size:
1819 1061 718 290 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 822 3924
Size: 822 3102
BstBI TT'CG_AA
Cuts at: 0 1194 3924
Size: 1194 2730
BstEII G'GTnAC_C
Cuts at: 0 270 315 3924
Size: 270 45 3609
Fragments arranged by size:
3609 270 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3606 3924
Size: 3606 318
BstYI r'GATC_y
Cuts at: 0 4 1504 1578 3013 3924
Size: 4 1500 74 1435 911
Fragments arranged by size:
1500 1435 911 74 4
Bsu36I CC'TnA_GG
Cuts at: 0 119 3924
Size: 119 3805
BtgI C'CryG_G
Cuts at: 0 2427 3924
Size: 2427 1497
BtsI GCAGTG_nn'
Cuts at: 0 697 1144 1360 3515 3770 3924
Size: 697 447 216 2155 255 154
Fragments arranged by size:
2155 697 447 255 216 154
DraI TTT'AAA
Cuts at: 0 3285 3924
Size: 3285 639
DraIII CAC_nnn'GTG
Cuts at: 0 1528 2634 3924
Size: 1528 1106 1290
Fragments arranged by size:
1528 1290 1106
EaeI y'GGCC_r
Cuts at: 0 281 1037 1410 1979 2762 3924
Size: 281 756 373 569 783 1162
Fragments arranged by size:
1162 783 756 569 373 281
EarI CTCTTCn'nnn_
Cuts at: 0 183 705 2752 3924
Size: 183 522 2047 1172
Fragments arranged by size:
2047 1172 522 183
EcoICRI GAG'CTC
Cuts at: 0 147 1127 3176 3924
Size: 147 980 2049 748
Fragments arranged by size:
2049 980 748 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 135 421 1113 1181 1219 1599 1609
Size: 114 21 286 692 68 38 380 10
Cuts at: 1609 1722 1793 1981 2837 3054 3556 3924
Size: 113 71 188 856 217 502 368
Fragments arranged by size:
856 692 502 380 368 286 217 188
114 113 71 68 38 21 10
EcoO109I rG'GnC_Cy
Cuts at: 0 1441 1601 2341 3924
Size: 1441 160 740 1583
Fragments arranged by size:
1583 1441 740 160
EcoRI G'AATT_C
Cuts at: 0 78 898 2376 3400 3924
Size: 78 820 1478 1024 524
Fragments arranged by size:
1478 1024 820 524 78
EcoRV GAT'ATC
Cuts at: 0 1026 3924
Size: 1026 2898
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1407 2290 3211 3924
Size: 1407 883 921 713
Fragments arranged by size:
1407 921 883 713
FspI TGC'GCA
Cuts at: 0 3482 3924
Size: 3482 442
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 677 709 1010 1042 3107 3139
Size: 580 32 65 32 301 32 2065 32
Cuts at: 3139 3616 3648 3924
Size: 477 32 276
Fragments arranged by size:
2065 580 477 301 276 65 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 852 3273 3924
Size: 852 2421 651
Fragments arranged by size:
2421 852 651
HindIII A'AGCT_T
Cuts at: 0 1497 3156 3924
Size: 1497 1659 768
Fragments arranged by size:
1659 1497 768
HpaI GTT'AAC
Cuts at: 0 3273 3924
Size: 3273 651
MfeI C'AATT_G
Cuts at: 0 2211 3924
Size: 2211 1713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 2563 3924
Size: 332 2231 1361
Fragments arranged by size:
2231 1361 332
MscI TGG'CCA
Cuts at: 0 283 1039 1981 3924
Size: 283 756 942 1943
Fragments arranged by size:
1943 942 756 283
MslI CAynn'nnrTG
Cuts at: 0 277 884 1066 1394 3449 3924
Size: 277 607 182 328 2055 475
Fragments arranged by size:
2055 607 475 328 277 182
MspA1I CmG'CkG
Cuts at: 0 277 1239 1565 2682 2710 3602 3924
Size: 277 962 326 1117 28 892 322
Fragments arranged by size:
1117 962 892 326 322 277 28
NdeI CA'TA_TG
Cuts at: 0 1956 3924
Size: 1956 1968
NspI r_CATG'y
Cuts at: 0 2722 3924
Size: 2722 1202
PasI CC'CwG_GG
Cuts at: 0 786 3924
Size: 786 3138
PflMI CCAn_nnn'nTGG
Cuts at: 0 837 952 1528 1960 3924
Size: 837 115 576 432 1964
Fragments arranged by size:
1964 837 576 432 115
PfoI T'CCnGG_A
Cuts at: 0 165 1129 1699 3924
Size: 165 964 570 2225
Fragments arranged by size:
2225 964 570 165
PmlI CAC'GTG
Cuts at: 0 3525 3588 3924
Size: 3525 63 336
Fragments arranged by size:
3525 336 63
PpuMI rG'GwC_Cy
Cuts at: 0 1601 3924
Size: 1601 2323
PsiI TTA'TAA
Cuts at: 0 1013 2264 2333 3924
Size: 1013 1251 69 1591
Fragments arranged by size:
1591 1251 1013 69
PstI C_TGCA'G
Cuts at: 0 2807 3510 3723 3924
Size: 2807 703 213 201
Fragments arranged by size:
2807 703 213 201
PvuII CAG'CTG
Cuts at: 0 1239 2682 3602 3924
Size: 1239 1443 920 322
Fragments arranged by size:
1443 1239 920 322
SacI G_AGCT'C
Cuts at: 0 149 1129 3178 3924
Size: 149 980 2049 746
Fragments arranged by size:
2049 980 746 149
SanDI GG'GwC_CC
Cuts at: 0 1601 3924
Size: 1601 2323
SapI GCTCTTCn'nnn_
Cuts at: 0 705 2752 3924
Size: 705 2047 1172
Fragments arranged by size:
2047 1172 705
ScaI AGT'ACT
Cuts at: 0 3916 3924
Size: 3916 8
SfcI C'TryA_G
Cuts at: 0 429 579 2803 3506 3719 3924
Size: 429 150 2224 703 213 205
Fragments arranged by size:
2224 703 429 213 205 150
SmlI C'TyrA_G
Cuts at: 0 25 287 1282 1392 3924
Size: 25 262 995 110 2532
Fragments arranged by size:
2532 995 262 110 25
StuI AGG'CCT
Cuts at: 0 2662 3924
Size: 2662 1262
StyI C'CwwG_G
Cuts at: 0 252 491 1605 3924
Size: 252 239 1114 2319
Fragments arranged by size:
2319 1114 252 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 303 1894 2616 2620 2743 2786 3575 3607
Size: 303 1591 722 4 123 43 789 32
Cuts at: 3607 3924
Size: 317
Fragments arranged by size:
1591 789 722 317 303 123 43 32
4
TatI w'GTAC_w
Cuts at: 0 425 2164 3914 3924
Size: 425 1739 1750 10
Fragments arranged by size:
1750 1739 425 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 947 979 1602 1634 3924
Size: 947 32 623 32 2290
Fragments arranged by size:
2290 947 623 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3568 3594 3924
Size: 3568 26 330
Fragments arranged by size:
3568 330 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 768 3924
Size: 768 3156
XmnI GAAnn'nnTTC
Cuts at: 0 567 1806 1856 2273 3924
Size: 567 1239 50 417 1651
Fragments arranged by size:
1651 1239 567 417 50
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AleI AlwNI ApoI
AseI BamHI BanI BanII BbvCI BciVI BclI BglII
BlpI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI Hin4I
HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I
NdeI NspI PasI PflMI PfoI PmlI PpuMI PsiI
PstI PvuII SacI SanDI SapI ScaI SfcI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AlfI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI
BbsI BcgI BfrBI BglI Bme1580I BmgBI BmtI BsaHI
BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstZ17I BtgZI ClaI CspCI DrdI EagI EciI
EcoNI FseI FspAI HaeII KasI KpnI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PciI PmeI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XhoI XmaI ZraI