Restriction map for
pF1KA1775
(Linear) (Six-base) MAPSORT of: KIAA1775.seq Check: 5129 from: 1 to: 2577
KIAA1775.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AccI GT'mk_AC
Cuts at: 0 161 2577
Size: 161 2416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1980 2577
Size: 817 1163 597
Fragments arranged by size:
1163 817 597
AflIII A'CryG_T
Cuts at: 0 1853 2577
Size: 1853 724
AleI CACnn'nnGTG
Cuts at: 0 560 1486 2134 2577
Size: 560 926 648 443
Fragments arranged by size:
926 648 560 443
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 517 549 1721 1753 2577
Size: 517 32 1172 32 824
Fragments arranged by size:
1172 824 517 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 184 496 528 1386 2035 2577
Size: 184 312 32 858 649 542
Fragments arranged by size:
858 649 542 312 184 32
ApaLI G'TGCA_C
Cuts at: 0 2557 2577
Size: 2557 20
ApoI r'AATT_y
Cuts at: 0 839 1180 1896 2577
Size: 839 341 716 681
Fragments arranged by size:
839 716 681 341
AseI AT'TA_AT
Cuts at: 0 884 2577
Size: 884 1693
AvaI C'yCGr_G
Cuts at: 0 433 2364 2577
Size: 433 1931 213
Fragments arranged by size:
1931 433 213
BamHI G'GATC_C
Cuts at: 0 1491 2577
Size: 1491 1086
BanI G'GyrC_C
Cuts at: 0 393 2119 2400 2577
Size: 393 1726 281 177
Fragments arranged by size:
1726 393 281 177
BanII G_rGCy'C
Cuts at: 0 787 922 979 991 1465 1847 1987 2056
Size: 787 135 57 12 474 382 140 69
Cuts at: 2056 2373 2531 2577
Size: 317 158 46
Fragments arranged by size:
787 474 382 317 158 140 135 69
57 46 12
BbeI G_GCGC'C
Cuts at: 0 397 2404 2577
Size: 397 2007 173
Fragments arranged by size:
2007 397 173
BbsI GAAGACnn'nnnn_
Cuts at: 0 726 1753 2577
Size: 726 1027 824
Fragments arranged by size:
1027 824 726
BbvCI CC'TCA_GC
Cuts at: 0 1613 1679 2577
Size: 1613 66 898
Fragments arranged by size:
1613 898 66
BglI GCCn_nnn'nGGC
Cuts at: 0 25 2208 2577
Size: 25 2183 369
Fragments arranged by size:
2183 369 25
BglII A'GATC_T
Cuts at: 0 1883 2577
Size: 1883 694
Bme1580I G_kGCm'C
Cuts at: 0 745 2561 2577
Size: 745 1816 16
Fragments arranged by size:
1816 745 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 1006 1550 1583 1762 2415 2577
Size: 1006 544 33 179 653 162
Fragments arranged by size:
1006 653 544 179 162 33
BmtI G_CTAG'C
Cuts at: 0 1604 2577
Size: 1604 973
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2061 2577
Size: 2061 516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 559 916 917 1449 2577
Size: 518 41 357 1 532 1128
Fragments arranged by size:
1128 532 518 357 41 1
Bpu10I CC'TnA_GC
Cuts at: 0 56 1613 1679 2577
Size: 56 1557 66 898
Fragments arranged by size:
1557 898 66 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1123 1876 1999 2251 2515 2577
Size: 1123 753 123 252 264 62
Fragments arranged by size:
1123 753 264 252 123 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 185 812 2032 2577
Size: 185 627 1220 545
Fragments arranged by size:
1220 627 545 185
BsaAI yAC'GTr
Cuts at: 0 159 2577
Size: 159 2418
BsaBI GATnn'nnATC
Cuts at: 0 2023 2577
Size: 2023 554
BsaHI Gr'CG_yC
Cuts at: 0 394 2401 2577
Size: 394 2007 176
Fragments arranged by size:
2007 394 176
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 491 517 521 547 1651 1681
Size: 184 30 277 26 4 26 1104 30
Cuts at: 1681 1969 1999 2577
Size: 288 30 578
Fragments arranged by size:
1104 578 288 277 184 30 30 30
26 26 4
BseYI C'CCAG_C
Cuts at: 0 223 448 580 926 990 1589 2230 2392
Size: 223 225 132 346 64 599 641 162
Cuts at: 2392 2404 2577
Size: 12 173
Fragments arranged by size:
641 599 346 225 223 173 162 132
64 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 1906 1987 2577
Size: 514 1392 81 590
Fragments arranged by size:
1392 590 514 81
BsiHKAI G_wGCw'C
Cuts at: 0 1109 2219 2281 2373 2531 2561 2577
Size: 1109 1110 62 92 158 30 16
Fragments arranged by size:
1110 1109 158 92 62 30 16
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2445 2577
Size: 2445 132
Bsp1286I G_dGCh'C
Cuts at: 0 745 787 922 979 991 1109 1465 1847
Size: 745 42 135 57 12 118 356 382
Cuts at: 1847 1987 2056 2219 2281 2373 2531 2561 2577
Size: 140 69 163 62 92 158 30 16
Fragments arranged by size:
745 382 356 163 158 140 135 118
92 69 62 57 42 30 16 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 453 536 2577
Size: 453 83 2041
Fragments arranged by size:
2041 453 83
BsrBI CCG'CTC
Cuts at: 0 1070 1907 2577
Size: 1070 837 670
Fragments arranged by size:
1070 837 670
BsrFI r'CCGG_y
Cuts at: 0 11 2116 2577
Size: 11 2105 461
Fragments arranged by size:
2105 461 11
BsrGI T'GTAC_A
Cuts at: 0 957 2577
Size: 957 1620
BstAPI GCAn_nnn'nTGC
Cuts at: 0 528 2577
Size: 528 2049
BstEII G'GTnAC_C
Cuts at: 0 372 2577
Size: 372 2205
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1473 1815 2136 2493 2577
Size: 1473 342 321 357 84
Fragments arranged by size:
1473 357 342 321 84
BstYI r'GATC_y
Cuts at: 0 1124 1491 1883 2577
Size: 1124 367 392 694
Fragments arranged by size:
1124 694 392 367
BstZ17I GTA'TAC
Cuts at: 0 162 2577
Size: 162 2415
Bsu36I CC'TnA_GG
Cuts at: 0 185 2577
Size: 185 2392
BtgI C'CryG_G
Cuts at: 0 545 626 689 809 2057 2102 2123 2577
Size: 545 81 63 120 1248 45 21 454
Fragments arranged by size:
1248 545 454 120 81 63 45 21
BtsI GCAGTG_nn'
Cuts at: 0 1619 1693 2577
Size: 1619 74 884
Fragments arranged by size:
1619 884 74
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1247 1282 2577
Size: 1247 35 1295
Fragments arranged by size:
1295 1247 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 91 2577
Size: 91 2486
EaeI y'GGCC_r
Cuts at: 0 350 2114 2126 2138 2577
Size: 350 1764 12 12 439
Fragments arranged by size:
1764 439 350 12 12
EarI CTCTTCn'nnn_
Cuts at: 0 275 1558 2545 2577
Size: 275 1283 987 32
Fragments arranged by size:
1283 987 275 32
EcoICRI GAG'CTC
Cuts at: 0 2371 2529 2577
Size: 2371 158 48
Fragments arranged by size:
2371 158 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 559 817 916 917 1449 1980 2577
Size: 518 41 258 99 1 532 531 597
Fragments arranged by size:
597 532 531 518 258 99 41 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 2577
Size: 133 2444
EcoO109I rG'GnC_Cy
Cuts at: 0 605 1134 1201 1504 1763 2196 2577
Size: 605 529 67 303 259 433 381
Fragments arranged by size:
605 529 433 381 303 259 67
EcoRI G'AATT_C
Cuts at: 0 839 1896 2577
Size: 839 1057 681
Fragments arranged by size:
1057 839 681
HaeII r_GCGC'y
Cuts at: 0 9 397 2404 2577
Size: 9 388 2007 173
Fragments arranged by size:
2007 388 173 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 182 184 214 216 478 491 510 523
Size: 182 2 30 2 262 13 19 13
Cuts at: 523 1969 2001 2577
Size: 1446 32 576
Fragments arranged by size:
1446 576 262 182 32 30 19 13
13 2 2
HincII GTy'rAC
Cuts at: 0 249 2513 2577
Size: 249 2264 64
Fragments arranged by size:
2264 249 64
KasI G'GCGC_C
Cuts at: 0 393 2400 2577
Size: 393 2007 177
Fragments arranged by size:
2007 393 177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1184 2577
Size: 1184 1393
MscI TGG'CCA
Cuts at: 0 2128 2140 2577
Size: 2128 12 437
Fragments arranged by size:
2128 437 12
MslI CAynn'nnrTG
Cuts at: 0 560 625 1486 2134 2577
Size: 560 65 861 648 443
Fragments arranged by size:
861 648 560 443 65
MspA1I CmG'CkG
Cuts at: 0 560 994 1194 1279 1366 1486 2577
Size: 560 434 200 85 87 120 1091
Fragments arranged by size:
1091 560 434 200 120 87 85
NaeI GCC'GGC
Cuts at: 0 2118 2577
Size: 2118 459
NarI GG'CG_CC
Cuts at: 0 394 2401 2577
Size: 394 2007 176
Fragments arranged by size:
2007 394 176
NcoI C'CATG_G
Cuts at: 0 809 2057 2123 2577
Size: 809 1248 66 454
Fragments arranged by size:
1248 809 454 66
NgoMIV G'CCGG_C
Cuts at: 0 2116 2577
Size: 2116 461
NheI G'CTAG_C
Cuts at: 0 1600 2577
Size: 1600 977
NspI r_CATG'y
Cuts at: 0 958 2577
Size: 958 1619
PasI CC'CwG_GG
Cuts at: 0 30 1116 1205 1456 1508 1595 2577
Size: 30 1086 89 251 52 87 982
Fragments arranged by size:
1086 982 251 89 87 52 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 477 1386 1573 2171 2577
Size: 477 909 187 598 406
Fragments arranged by size:
909 598 477 406 187
PfoI T'CCnGG_A
Cuts at: 0 407 607 1385 2047 2577
Size: 407 200 778 662 530
Fragments arranged by size:
778 662 530 407 200
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 2577
Size: 517 32 2028
Fragments arranged by size:
2028 517 32
PpuMI rG'GwC_Cy
Cuts at: 0 605 1201 1504 1763 2196 2577
Size: 605 596 303 259 433 381
Fragments arranged by size:
605 596 433 381 303 259
PstI C_TGCA'G
Cuts at: 0 524 2577
Size: 524 2053
PvuII CAG'CTG
Cuts at: 0 994 1194 1279 1486 2577
Size: 994 200 85 207 1091
Fragments arranged by size:
1091 994 207 200 85
SacI G_AGCT'C
Cuts at: 0 2373 2531 2577
Size: 2373 158 46
Fragments arranged by size:
2373 158 46
SanDI GG'GwC_CC
Cuts at: 0 1201 1763 2577
Size: 1201 562 814
Fragments arranged by size:
1201 814 562
SapI GCTCTTCn'nnn_
Cuts at: 0 275 2577
Size: 275 2302
SexAI A'CCwGG_T
Cuts at: 0 1814 2577
Size: 1814 763
SfcI C'TryA_G
Cuts at: 0 146 520 846 2577
Size: 146 374 326 1731
Fragments arranged by size:
1731 374 326 146
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 25 2577
Size: 25 2552
SfoI GGC'GCC
Cuts at: 0 395 2402 2577
Size: 395 2007 175
Fragments arranged by size:
2007 395 175
SmlI C'TyrA_G
Cuts at: 0 1138 1891 2014 2266 2530 2577
Size: 1138 753 123 252 264 47
Fragments arranged by size:
1138 753 264 252 123 47
SspI AAT'ATT
Cuts at: 0 1525 2577
Size: 1525 1052
StyI C'CwwG_G
Cuts at: 0 635 809 1973 2057 2081 2123 2577
Size: 635 174 1164 84 24 42 454
Fragments arranged by size:
1164 635 454 174 84 42 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 391 1124 1580 1603 1849 2095 2406 2577
Size: 391 733 456 23 246 246 311 171
Fragments arranged by size:
733 456 391 311 246 246 171 23
TatI w'GTAC_w
Cuts at: 0 957 1667 2577
Size: 957 710 910
Fragments arranged by size:
957 910 710
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1220 1252 2577
Size: 1220 32 1325
Fragments arranged by size:
1325 1220 32
Tth111I GACn'n_nGTC
Cuts at: 0 1402 2577
Size: 1402 1175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 111 1026 1570 1815 1908 2100 2577
Size: 111 915 544 245 93 192 477
Fragments arranged by size:
915 544 477 245 192 111 93
Enzymes that do cut:
AccI AcuI AflIII AleI AloI AlwNI ApaLI ApoI
AseI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BglI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI
DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PflMI
PfoI PpiI PpuMI PstI PvuII SacI SanDI SapI
SexAI SfcI SfiI SfoI SmlI SspI StyI TaqII
TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaI AscI AsiSI AvrII BaeI BcgI BciVI
BclI BfrBI BlpI BmgBI BsaWI BseRI BsiEI BsiWI
BsmI BspEI BspHI BsrDI BssHII BssSI BstBI BtgZI
ClaI DraI DraIII EagI EciI EcoRV FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI
NotI NruI NsiI PacI PciI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SbfI ScaI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI XbaI XhoI XmaI XmnI ZraI