Restriction map for
pF1KSDA1778
(Linear) (Six-base) MAPSORT of: KIAA1778.seq Check: 4269 from: 1 to: 975
KIAA1778.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 904 975
Size: 904 71
AatII G_ACGT'C
Cuts at: 0 727 975
Size: 727 248
AfeI AGC'GCT
Cuts at: 0 793 975
Size: 793 182
AflIII A'CryG_T
Cuts at: 0 19 975
Size: 19 956
AhdI GACnn_n'nnGTC
Cuts at: 0 849 975
Size: 849 126
AleI CACnn'nnGTG
Cuts at: 0 154 975
Size: 154 821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 352 975
Size: 352 623
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 670 702 975
Size: 670 32 273
Fragments arranged by size:
670 273 32
AlwNI CAG_nnn'CTG
Cuts at: 0 213 600 975
Size: 213 387 375
Fragments arranged by size:
387 375 213
ApaI G_GGCC'C
Cuts at: 0 577 975
Size: 577 398
ApaLI G'TGCA_C
Cuts at: 0 436 975
Size: 436 539
AvaI C'yCGr_G
Cuts at: 0 218 322 385 408 618 975
Size: 218 104 63 23 210 357
Fragments arranged by size:
357 218 210 104 63 23
BanI G'GyrC_C
Cuts at: 0 162 390 505 519 892 975
Size: 162 228 115 14 373 83
Fragments arranged by size:
373 228 162 115 83 14
BanII G_rGCy'C
Cuts at: 0 374 451 491 577 975
Size: 374 77 40 86 398
Fragments arranged by size:
398 374 86 77 40
BbeI G_GCGC'C
Cuts at: 0 394 509 523 975
Size: 394 115 14 452
Fragments arranged by size:
452 394 115 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 954 975
Size: 954 21
BglI GCCn_nnn'nGGC
Cuts at: 0 426 975
Size: 426 549
BglII A'GATC_T
Cuts at: 0 935 975
Size: 935 40
Bme1580I G_kGCm'C
Cuts at: 0 167 440 577 895 975
Size: 167 273 137 318 80
Fragments arranged by size:
318 273 167 137 80
BmrI ACTGGGnnnn_n'
Cuts at: 0 588 975
Size: 588 387
BmtI G_CTAG'C
Cuts at: 0 548 975
Size: 548 427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 525 975
Size: 525 450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 850 975
Size: 556 294 125
Fragments arranged by size:
556 294 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 975
Size: 799 176
BsaAI yAC'GTr
Cuts at: 0 22 135 975
Size: 22 113 840
Fragments arranged by size:
840 113 22
BsaHI Gr'CG_yC
Cuts at: 0 391 506 520 680 724 975
Size: 391 115 14 160 44 251
Fragments arranged by size:
391 251 160 115 44 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 631 661 975
Size: 631 30 314
Fragments arranged by size:
631 314 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 54 975
Size: 54 921
BseYI C'CCAG_C
Cuts at: 0 395 453 975
Size: 395 58 522
Fragments arranged by size:
522 395 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 900 975
Size: 132 768 75
Fragments arranged by size:
768 132 75
BsiEI CG_ry'CG
Cuts at: 0 780 975
Size: 780 195
BsiHKAI G_wGCw'C
Cuts at: 0 374 440 975
Size: 374 66 535
Fragments arranged by size:
535 374 66
Bsp1286I G_dGCh'C
Cuts at: 0 167 374 440 451 491 577 895 975
Size: 167 207 66 11 40 86 318 80
Fragments arranged by size:
318 207 167 86 80 66 40 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 904 975
Size: 293 611 71
Fragments arranged by size:
611 293 71
BsrBI CCG'CTC
Cuts at: 0 90 975
Size: 90 885
BsrFI r'CCGG_y
Cuts at: 0 332 611 975
Size: 332 279 364
Fragments arranged by size:
364 332 279
BssHII G'CGCG_C
Cuts at: 0 700 975
Size: 700 275
BssSI C'ACGA_G
Cuts at: 0 301 373 975
Size: 301 72 602
Fragments arranged by size:
602 301 72
BstAPI GCAn_nnn'nTGC
Cuts at: 0 303 975
Size: 303 672
BstYI r'GATC_y
Cuts at: 0 935 975
Size: 935 40
BtgI C'CryG_G
Cuts at: 0 77 642 887 975
Size: 77 565 245 88
Fragments arranged by size:
565 245 88 77
BtsI GCAGTG_nn'
Cuts at: 0 63 975
Size: 63 912
DraIII CAC_nnn'GTG
Cuts at: 0 873 975
Size: 873 102
EaeI y'GGCC_r
Cuts at: 0 731 975
Size: 731 244
EarI CTCTTCn'nnn_
Cuts at: 0 97 268 975
Size: 97 171 707
Fragments arranged by size:
707 171 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 691 975
Size: 25 666 284
Fragments arranged by size:
666 284 25
EcoICRI GAG'CTC
Cuts at: 0 372 975
Size: 372 603
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 850 975
Size: 556 294 125
Fragments arranged by size:
556 294 125
EcoNI CCTnn'n_nnAGG
Cuts at: 0 832 975
Size: 832 143
EcoO109I rG'GnC_Cy
Cuts at: 0 771 975
Size: 771 204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 840 975
Size: 840 135
HaeII r_GCGC'y
Cuts at: 0 147 394 509 523 618 795 975
Size: 147 247 115 14 95 177 180
Fragments arranged by size:
247 180 177 147 115 95 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 835 867 975
Size: 835 32 108
Fragments arranged by size:
835 108 32
KasI G'GCGC_C
Cuts at: 0 390 505 519 975
Size: 390 115 14 456
Fragments arranged by size:
456 390 115 14
MscI TGG'CCA
Cuts at: 0 733 975
Size: 733 242
MslI CAynn'nnrTG
Cuts at: 0 154 975
Size: 154 821
MspA1I CmG'CkG
Cuts at: 0 79 579 644 975
Size: 79 500 65 331
Fragments arranged by size:
500 331 79 65
NaeI GCC'GGC
Cuts at: 0 334 613 975
Size: 334 279 362
Fragments arranged by size:
362 334 279
NarI GG'CG_CC
Cuts at: 0 391 506 520 975
Size: 391 115 14 455
Fragments arranged by size:
455 391 115 14
NgoMIV G'CCGG_C
Cuts at: 0 332 611 975
Size: 332 279 364
Fragments arranged by size:
364 332 279
NheI G'CTAG_C
Cuts at: 0 544 975
Size: 544 431
NspI r_CATG'y
Cuts at: 0 189 975
Size: 189 786
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 873 975
Size: 600 273 102
Fragments arranged by size:
600 273 102
PfoI T'CCnGG_A
Cuts at: 0 275 827 975
Size: 275 552 148
Fragments arranged by size:
552 275 148
PmlI CAC'GTG
Cuts at: 0 22 975
Size: 22 953
PpuMI rG'GwC_Cy
Cuts at: 0 771 975
Size: 771 204
PspOMI G'GGCC_C
Cuts at: 0 573 975
Size: 573 402
PstI C_TGCA'G
Cuts at: 0 341 970 975
Size: 341 629 5
Fragments arranged by size:
629 341 5
SacI G_AGCT'C
Cuts at: 0 374 975
Size: 374 601
SacII CC_GC'GG
Cuts at: 0 80 645 975
Size: 80 565 330
Fragments arranged by size:
565 330 80
SanDI GG'GwC_CC
Cuts at: 0 771 975
Size: 771 204
SapI GCTCTTCn'nnn_
Cuts at: 0 97 975
Size: 97 878
ScaI AGT'ACT
Cuts at: 0 355 975
Size: 355 620
SfcI C'TryA_G
Cuts at: 0 337 966 975
Size: 337 629 9
Fragments arranged by size:
629 337 9
SfoI GGC'GCC
Cuts at: 0 392 507 521 975
Size: 392 115 14 454
Fragments arranged by size:
454 392 115 14
SmaI CCC'GGG
Cuts at: 0 220 410 975
Size: 220 190 565
Fragments arranged by size:
565 220 190
SmlI C'TyrA_G
Cuts at: 0 814 975
Size: 814 161
StuI AGG'CCT
Cuts at: 0 838 975
Size: 838 137
StyI C'CwwG_G
Cuts at: 0 734 975
Size: 734 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 445 975
Size: 445 530
TatI w'GTAC_w
Cuts at: 0 353 975
Size: 353 622
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 77 109 975
Size: 77 32 866
Fragments arranged by size:
866 77 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 537 975
Size: 537 438
XmaI C'CCGG_G
Cuts at: 0 218 408 975
Size: 218 190 567
Fragments arranged by size:
567 218 190
ZraI GAC'GTC
Cuts at: 0 725 975
Size: 725 250
Enzymes that do cut:
AarI AatII AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BglI BglII Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstYI
BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I KasI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NspI PflMI PfoI
PmlI PpuMI PspOMI PstI SacI SacII SanDI SapI
ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AflII AgeI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BlpI BmgBI Bpu10I BsaI BsaBI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI
NotI NruI NsiI PacI PasI PciI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI
SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI