Restriction map for
pF1KSDA1783
(Linear) (Six-base) MAPSORT of: KIAA1783.seq Check: 5682 from: 1 to: 9288
KIAA1783.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 918 4237 8408 8835 9125 9288
Size: 918 3319 4171 427 290 163
Fragments arranged by size:
4171 3319 918 427 290 163
AccI GT'mk_AC
Cuts at: 0 4205 8215 9288
Size: 4205 4010 1073
Fragments arranged by size:
4205 4010 1073
Acc65I G'GTAC_C
Cuts at: 0 1189 3060 4298 9288
Size: 1189 1871 1238 4990
Fragments arranged by size:
4990 1871 1238 1189
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1706 2905 4510 5124 6068 6933 7300 7378
Size: 1706 1199 1605 614 944 865 367 78
Cuts at: 7378 8098 8689 9115 9288
Size: 720 591 426 173
Fragments arranged by size:
1706 1605 1199 944 865 720 614 591
426 367 173 78
AfeI AGC'GCT
Cuts at: 0 602 4189 5003 9288
Size: 602 3587 814 4285
Fragments arranged by size:
4285 3587 814 602
AflII C'TTAA_G
Cuts at: 0 7435 9288
Size: 7435 1853
AflIII A'CryG_T
Cuts at: 0 446 3915 9288
Size: 446 3469 5373
Fragments arranged by size:
5373 3469 446
AgeI A'CCGG_T
Cuts at: 0 2288 9288
Size: 2288 7000
AleI CACnn'nnGTG
Cuts at: 0 2447 3069 3274 3566 9288
Size: 2447 622 205 292 5722
Fragments arranged by size:
5722 2447 622 292 205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5022 7216 7634 9288
Size: 5022 2194 418 1654
Fragments arranged by size:
5022 2194 1654 418
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 8842 8874 9288
Size: 8842 32 414
Fragments arranged by size:
8842 414 32
AlwNI CAG_nnn'CTG
Cuts at: 0 33 244 473 635 3375 3637 3816 4026
Size: 33 211 229 162 2740 262 179 210
Cuts at: 4026 4092 4164 4200 4359 4380 4665 5728 6726
Size: 66 72 36 159 21 285 1063 998
Cuts at: 6726 8691 8811 8886 9006 9075 9222 9288
Size: 1965 120 75 120 69 147 66
Fragments arranged by size:
2740 1965 1063 998 285 262 229 211
210 179 162 159 147 120 120 75
72 69 66 66 36 33 21
ApaI G_GGCC'C
Cuts at: 0 305 401 686 1072 4905 5070 5618 6478
Size: 305 96 285 386 3833 165 548 860
Cuts at: 6478 6622 6671 6701 9146 9288
Size: 144 49 30 2445 142
Fragments arranged by size:
3833 2445 860 548 386 305 285 165
144 142 96 49 30
ApoI r'AATT_y
Cuts at: 0 7834 8512 9288
Size: 7834 678 776
Fragments arranged by size:
7834 776 678
AscI GG'CGCG_CC
Cuts at: 0 95 9288
Size: 95 9193
AvaI C'yCGr_G
Cuts at: 0 43 530 653 723 758 806 890 1471
Size: 43 487 123 70 35 48 84 581
Cuts at: 1471 2007 5052 5063 5237 5909 6471 6778 7136
Size: 536 3045 11 174 672 562 307 358
Cuts at: 7136 7179 7388 8039 8168 9288
Size: 43 209 651 129 1120
Fragments arranged by size:
3045 1120 672 651 581 562 536 487
358 307 209 174 129 123 84 70
48 43 43 35 11
AvrII C'CTAG_G
Cuts at: 0 1939 9288
Size: 1939 7349
BamHI G'GATC_C
Cuts at: 0 1646 4753 5204 9288
Size: 1646 3107 451 4084
Fragments arranged by size:
4084 3107 1646 451
BanI G'GyrC_C
Cuts at: 0 323 856 930 1048 1189 1481 1948 2109
Size: 323 533 74 118 141 292 467 161
Cuts at: 2109 2125 3060 3234 3262 3423 3961 4072 4298
Size: 16 935 174 28 161 538 111 226
Cuts at: 4298 5229 5605 5748 6574 7891 8324 9288
Size: 931 376 143 826 1317 433 964
Fragments arranged by size:
1317 964 935 931 826 538 533 467
433 376 323 292 226 174 161 161
143 141 118 111 74 28 16
BanII G_rGCy'C
Cuts at: 0 305 401 571 686 757 804 897 1072
Size: 305 96 170 115 71 47 93 175
Cuts at: 1072 1119 1472 1898 1921 2903 3761 4175 4838
Size: 47 353 426 23 982 858 414 663
Cuts at: 4838 4905 5070 5580 5618 6478 6622 6671 6701
Size: 67 165 510 38 860 144 49 30
Cuts at: 6701 9032 9146 9288
Size: 2331 114 142
Fragments arranged by size:
2331 982 860 858 663 510 426 414
353 305 175 170 165 144 142 115
114 96 93 71 67 49 47 47
38 30 23
BbeI G_GCGC'C
Cuts at: 0 327 860 1052 1952 2113 5233 5609 8328
Size: 327 533 192 900 161 3120 376 2719
Cuts at: 8328 9288
Size: 960
Fragments arranged by size:
3120 2719 960 900 533 376 327 192
161
BbsI GAAGACnn'nnnn_
Cuts at: 0 2165 2476 2622 4052 4498 6056 7109 9231
Size: 2165 311 146 1430 446 1558 1053 2122
Cuts at: 9231 9288
Size: 57
Fragments arranged by size:
2165 2122 1558 1430 1053 446 311 146
57
BbvCI CC'TCA_GC
Cuts at: 0 2347 3434 4703 5554 5943 6317 7498 7957
Size: 2347 1087 1269 851 389 374 1181 459
Cuts at: 7957 8101 8185 8773 9288
Size: 144 84 588 515
Fragments arranged by size:
2347 1269 1181 1087 851 588 515 459
389 374 144 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1011 1045 2124 2158 3318 3352 5890 5924
Size: 1011 34 1079 34 1160 34 2538 34
Cuts at: 5924 8367 8401 9288
Size: 2443 34 887
Fragments arranged by size:
2538 2443 1160 1079 1011 887 34 34
34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3505 7027 9288
Size: 3505 3522 2261
Fragments arranged by size:
3522 3505 2261
BclI T'GATC_A
Cuts at: 0 1596 5312 9288
Size: 1596 3716 3976
Fragments arranged by size:
3976 3716 1596
BfrBI ATG'CAT
Cuts at: 0 2423 9288
Size: 2423 6865
BglI GCCn_nnn'nGGC
Cuts at: 0 48 521 886 1000 1082 1505 2377 2621
Size: 48 473 365 114 82 423 872 244
Cuts at: 2621 2935 4009 4895 4909 5056 5065 5510 5920
Size: 314 1074 886 14 147 9 445 410
Cuts at: 5920 6417 6473 6504 7334 7405 7557 8755 9288
Size: 497 56 31 830 71 152 1198 533
Fragments arranged by size:
1198 1074 886 872 830 533 497 473
445 423 410 365 314 244 152 147
114 82 71 56 48 31 14 9
BglII A'GATC_T
Cuts at: 0 5525 9288
Size: 5525 3763
BlpI GC'TnA_GC
Cuts at: 0 1509 2086 2723 4472 4921 5379 6323 9121
Size: 1509 577 637 1749 449 458 944 2798
Cuts at: 9121 9171 9288
Size: 50 117
Fragments arranged by size:
2798 1749 1509 944 637 577 458 449
117 50
Bme1580I G_kGCm'C
Cuts at: 0 305 401 686 1072 1754 2456 2914 3964
Size: 305 96 285 386 682 702 458 1050
Cuts at: 3964 3995 4713 4905 5070 5618 5722 6478 6491
Size: 31 718 192 165 548 104 756 13
Cuts at: 6491 6579 6622 6671 6701 9146 9288
Size: 88 43 49 30 2445 142
Fragments arranged by size:
2445 1050 756 718 702 682 548 458
386 305 285 192 165 142 104 96
88 49 43 31 30 13
BmgBI CAC'GTC
Cuts at: 0 449 3909 7200 8604 9288
Size: 449 3460 3291 1404 684
Fragments arranged by size:
3460 3291 1404 684 449
BmrI ACTGGGnnnn_n'
Cuts at: 0 824 1570 2417 3756 7155 8640 9288
Size: 824 746 847 1339 3399 1485 648
Fragments arranged by size:
3399 1485 1339 847 824 746 648
BmtI G_CTAG'C
Cuts at: 0 8818 9288
Size: 8818 470
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 810 1872 2805 5172 9288
Size: 810 1062 933 2367 4116
Fragments arranged by size:
4116 2367 1062 933 810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 349 625 759 1598 1842 2089 2101
Size: 55 294 276 134 839 244 247 12
Cuts at: 2101 2125 2533 2682 3097 3337 3348 3529 3709
Size: 24 408 149 415 240 11 181 180
Cuts at: 3709 3838 3970 4186 4348 4593 4780 5107 5245
Size: 129 132 216 162 245 187 327 138
Cuts at: 5245 5247 5426 5896 7366 7567 8196 8284 9151
Size: 2 179 470 1470 201 629 88 867
Cuts at: 9151 9288
Size: 137
Fragments arranged by size:
1470 867 839 629 470 415 408 327
294 276 247 245 244 240 216 201
187 181 180 179 162 149 138 137
134 132 129 88 55 24 12 11
2
Bpu10I CC'TnA_GC
Cuts at: 0 1920 2347 2463 2503 3434 4081 4703 5554
Size: 1920 427 116 40 931 647 622 851
Cuts at: 5554 5943 6317 7498 7957 8101 8185 8773 9288
Size: 389 374 1181 459 144 84 588 515
Fragments arranged by size:
1920 1181 931 851 647 622 588 515
459 427 389 374 144 116 84 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1072 2203 2710 4948 7225 9288
Size: 1072 1131 507 2238 2277 2063
Fragments arranged by size:
2277 2238 2063 1131 1072 507
BsaI GGTCTCn'nnnn_
Cuts at: 0 1873 1975 1997 2686 3129 6025 6849 8629
Size: 1873 102 22 689 443 2896 824 1780
Cuts at: 8629 9288
Size: 659
Fragments arranged by size:
2896 1873 1780 824 689 659 443 102
22
BsaAI yAC'GTr
Cuts at: 0 8823 9288
Size: 8823 465
BsaHI Gr'CG_yC
Cuts at: 0 234 324 424 857 1049 1666 1949 2110
Size: 234 90 100 433 192 617 283 161
Cuts at: 2110 3109 3167 3503 4514 5230 5606 8325 9288
Size: 999 58 336 1011 716 376 2719 963
Fragments arranged by size:
2719 1011 999 963 716 617 433 376
336 283 234 192 161 100 90 58
BsaWI w'CCGG_w
Cuts at: 0 346 477 739 1230 2043 2288 2802 7019
Size: 346 131 262 491 813 245 514 4217
Cuts at: 7019 7585 7853 9010 9288
Size: 566 268 1157 278
Fragments arranged by size:
4217 1157 813 566 514 491 346 278
268 262 245 131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 61 85 91 2657 2687 3310 3340
Size: 55 6 24 6 2566 30 623 30
Cuts at: 3340 3382 3400 3412 3430 6988 7018 7920 7950
Size: 42 18 12 18 3558 30 902 30
Cuts at: 7950 8455 8485 8842 8872 9005 9035 9049 9079
Size: 505 30 357 30 133 30 14 30
Cuts at: 9079 9288
Size: 209
Fragments arranged by size:
3558 2566 902 623 505 357 209 133
55 42 30 30 30 30 30 30
30 30 24 18 18 14 12 6
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 365 794 1018 2427 2932 2970 2973
Size: 346 19 429 224 1409 505 38 3
Cuts at: 2973 3228 3403 3406 3409 4183 5161 5360 6055
Size: 255 175 3 3 774 978 199 695
Cuts at: 6055 6196 6199 6202 6205 6208 6211 6214 6217
Size: 141 3 3 3 3 3 3 3
Cuts at: 6217 6220 6223 6232 6245 6248 6629 6964 7486
Size: 3 3 9 13 3 381 335 522
Cuts at: 7486 7668 7945 8479 9148 9288
Size: 182 277 534 669 140
Fragments arranged by size:
1409 978 774 695 669 534 522 505
429 381 346 335 277 255 224 199
182 175 141 140 38 19 13 9
3 3 3 3 3 3 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 26 100 244 360 732 990 1518 1907
Size: 26 74 144 116 372 258 528 389
Cuts at: 1907 2961 3023 3577 3837 4092 4352 4449 4522
Size: 1054 62 554 260 255 260 97 73
Cuts at: 4522 4567 4581 4682 4856 4898 5022 5081 5197
Size: 45 14 101 174 42 124 59 116
Cuts at: 5197 5340 5464 5573 5770 5840 6427 6561 6604
Size: 143 124 109 197 70 587 134 43
Cuts at: 6604 6851 7059 7314 7872 8263 9061 9277 9288
Size: 247 208 255 558 391 798 216 11
Fragments arranged by size:
1054 798 587 558 554 528 391 389
372 260 260 258 255 255 247 216
208 197 174 144 143 134 124 124
116 116 109 101 97 74 73 70
62 59 45 43 42 26 14 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1757 1785 1808 3916 4195 5242 5675 6514
Size: 1757 28 23 2108 279 1047 433 839
Cuts at: 6514 7222 7363 7630 7942 8148 8479 8527 8596
Size: 708 141 267 312 206 331 48 69
Cuts at: 8596 8629 9288
Size: 33 659
Fragments arranged by size:
2108 1757 1047 839 708 659 433 331
312 279 267 206 141 69 48 33
28 23
BsiEI CG_ry'CG
Cuts at: 0 123 155 315 369 1168 1634 2098 5162
Size: 123 32 160 54 799 466 464 3064
Cuts at: 5162 8155 9288
Size: 2993 1133
Fragments arranged by size:
3064 2993 1133 799 466 464 160 123
54 32
BsiHKAI G_wGCw'C
Cuts at: 0 2437 4175 4838 8297 9288
Size: 2437 1738 663 3459 991
Fragments arranged by size:
3459 2437 1738 991 663
BsmI GAATG_Cn'
Cuts at: 0 702 2931 5408 9288
Size: 702 2229 2477 3880
Fragments arranged by size:
3880 2477 2229 702
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2141 3100 8883 9288
Size: 2141 959 5783 405
Fragments arranged by size:
5783 2141 959 405
Bsp1286I G_dGCh'C
Cuts at: 0 305 401 571 686 757 804 897 1072
Size: 305 96 170 115 71 47 93 175
Cuts at: 1072 1119 1472 1754 1898 1921 2437 2456 2903
Size: 47 353 282 144 23 516 19 447
Cuts at: 2903 2914 3761 3964 3995 4175 4713 4838 4905
Size: 11 847 203 31 180 538 125 67
Cuts at: 4905 5070 5580 5618 5722 6478 6491 6579 6622
Size: 165 510 38 104 756 13 88 43
Cuts at: 6622 6671 6701 8297 9032 9146 9288
Size: 49 30 1596 735 114 142
Fragments arranged by size:
1596 847 756 735 538 516 510 447
353 305 282 203 180 175 170 165
144 142 125 115 114 104 96 93
88 71 67 49 47 47 43 38
31 30 23 19 13 11
BspEI T'CCGG_A
Cuts at: 0 346 477 7019 7585 7853 9010 9288
Size: 346 131 6542 566 268 1157 278
Fragments arranged by size:
6542 1157 566 346 278 268 131
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 918 1551 4217 4237 7064 8124 8408 8819
Size: 918 633 2666 20 2827 1060 284 411
Cuts at: 8819 8835 9125 9288
Size: 16 290 163
Fragments arranged by size:
2827 2666 1060 918 633 411 290 284
163 20 16
BsrBI CCG'CTC
Cuts at: 0 509 976 1095 1553 1853 2727 2778 2784
Size: 509 467 119 458 300 874 51 6
Cuts at: 2784 7038 7135 7624 9288
Size: 4254 97 489 1664
Fragments arranged by size:
4254 1664 874 509 489 467 458 300
119 97 51 6
BsrDI GCAATG_nn'
Cuts at: 0 5720 9086 9288
Size: 5720 3366 202
Fragments arranged by size:
5720 3366 202
BsrFI r'CCGG_y
Cuts at: 0 51 147 819 2288 3203 6892 7533 7614
Size: 51 96 672 1469 915 3689 641 81
Cuts at: 7614 8327 8474 8618 9288
Size: 713 147 144 670
Fragments arranged by size:
3689 1469 915 713 672 670 641 147
144 96 81 51
BssHII G'CGCG_C
Cuts at: 0 95 9288
Size: 95 9193
BssSI C'ACGA_G
Cuts at: 0 1303 1357 1411 1733 1930 9288
Size: 1303 54 54 322 197 7358
Fragments arranged by size:
7358 1303 322 197 54 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2972 4380 5019 5886 6878 7374 8811 9288
Size: 2972 1408 639 867 992 496 1437 477
Fragments arranged by size:
2972 1437 1408 992 867 639 496 477
BstEII G'GTnAC_C
Cuts at: 0 3568 7245 8023 9288
Size: 3568 3677 778 1265
Fragments arranged by size:
3677 3568 1265 778
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2449 4344 4690 4995 7885 8001 9024 9288
Size: 2449 1895 346 305 2890 116 1023 264
Fragments arranged by size:
2890 2449 1895 1023 346 305 264 116
BstYI r'GATC_y
Cuts at: 0 1617 1646 3032 4463 4753 5204 5525 6986
Size: 1617 29 1386 1431 290 451 321 1461
Cuts at: 6986 8058 9288
Size: 1072 1230
Fragments arranged by size:
1617 1461 1431 1386 1230 1072 451 321
290 29
Bsu36I CC'TnA_GG
Cuts at: 0 57 2026 2312 4550 6025 6523 6967 8498
Size: 57 1969 286 2238 1475 498 444 1531
Cuts at: 8498 8799 9288
Size: 301 489
Fragments arranged by size:
2238 1969 1531 1475 498 489 444 301
286 57
BtgI C'CryG_G
Cuts at: 0 123 315 1064 1198 1275 1329 1709 2016
Size: 123 192 749 134 77 54 380 307
Cuts at: 2016 3140 3527 3564 3956 5636 5741 6354 6827
Size: 1124 387 37 392 1680 105 613 473
Cuts at: 6827 6937 7224 7509 9288
Size: 110 287 285 1779
Fragments arranged by size:
1779 1680 1124 749 613 473 392 387
380 307 287 285 192 134 123 110
105 77 54 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 7443 9288
Size: 7443 1845
BtsI GCAGTG_nn'
Cuts at: 0 206 1869 2442 2454 2890 3975 4816 4936
Size: 206 1663 573 12 436 1085 841 120
Cuts at: 4936 4957 5254 5422 6248 6766 8160 8641 9261
Size: 21 297 168 826 518 1394 481 620
Cuts at: 9261 9288
Size: 27
Fragments arranged by size:
1663 1394 1085 841 826 620 573 518
481 436 297 206 168 120 27 21
12
DraI TTT'AAA
Cuts at: 0 3665 9288
Size: 3665 5623
DraIII CAC_nnn'GTG
Cuts at: 0 4538 5998 7756 8347 9272 9288
Size: 4538 1460 1758 591 925 16
Fragments arranged by size:
4538 1758 1460 925 591 16
DrdI GACnn_nn'nnGTC
Cuts at: 0 3516 6841 9288
Size: 3516 3325 2447
Fragments arranged by size:
3516 3325 2447
EaeI y'GGCC_r
Cuts at: 0 80 134 152 192 312 366 821 1165
Size: 80 54 18 40 120 54 455 344
Cuts at: 1165 1548 2095 2580 2622 2945 3117 3173 3711
Size: 383 547 485 42 323 172 56 538
Cuts at: 3711 4010 4478 4816 5096 5159 5792 6146 6344
Size: 299 468 338 280 63 633 354 198
Cuts at: 6344 6357 6418 6554 6717 7130 7538 8152 8756
Size: 13 61 136 163 413 408 614 604
Cuts at: 8756 9288
Size: 532
Fragments arranged by size:
633 614 604 547 538 532 485 468
455 413 408 383 354 344 338 323
299 280 198 172 163 136 120 80
63 61 56 54 54 42 40 18
13
EagI C'GGCC_G
Cuts at: 0 152 312 366 1165 2095 5159 8152 9288
Size: 152 160 54 799 930 3064 2993 1136
Fragments arranged by size:
3064 2993 1136 930 799 160 152 54
EarI CTCTTCn'nnn_
Cuts at: 0 350 2218 3202 3364 3544 7354 8527 8533
Size: 350 1868 984 162 180 3810 1173 6
Cuts at: 8533 8965 9091 9288
Size: 432 126 197
Fragments arranged by size:
3810 1868 1173 984 432 350 197 180
162 126 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1885 4513 7056 7255 8346 9288
Size: 1885 2628 2543 199 1091 942
Fragments arranged by size:
2628 2543 1885 1091 942 199
EcoICRI GAG'CTC
Cuts at: 0 4173 4836 9288
Size: 4173 663 4452
Fragments arranged by size:
4452 4173 663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 349 625 759 1598 1706 1842 2089
Size: 55 294 276 134 839 108 136 247
Cuts at: 2089 2101 2125 2533 2682 2905 3097 3337 3348
Size: 12 24 408 149 223 192 240 11
Cuts at: 3348 3529 3709 3838 3970 4186 4348 4510 4593
Size: 181 180 129 132 216 162 162 83
Cuts at: 4593 4780 5107 5124 5245 5247 5426 5896 6068
Size: 187 327 17 121 2 179 470 172
Cuts at: 6068 6933 7300 7366 7378 7567 8098 8196 8284
Size: 865 367 66 12 189 531 98 88
Cuts at: 8284 8689 9115 9151 9288
Size: 405 426 36 137
Fragments arranged by size:
865 839 531 470 426 408 405 367
327 294 276 247 240 223 216 192
189 187 181 180 179 172 162 162
149 137 136 134 132 129 121 108
98 88 83 66 55 36 24 17
12 12 11 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 331 1736 1993 2515 2824 3175 3745 4084
Size: 331 1405 257 522 309 351 570 339
Cuts at: 4084 4141 4770 6919 8776 9288
Size: 57 629 2149 1857 512
Fragments arranged by size:
2149 1857 1405 629 570 522 512 351
339 331 309 257 57
EcoO109I rG'GnC_Cy
Cuts at: 0 397 398 682 683 779 987 1037 1069
Size: 397 1 284 1 96 208 50 32
Cuts at: 1069 1563 1924 1936 2021 2076 2169 2260 2343
Size: 494 361 12 85 55 93 91 83
Cuts at: 2343 2415 3297 3813 3822 4023 4134 4197 4421
Size: 72 882 516 9 201 111 63 224
Cuts at: 4421 4902 4983 5027 5328 5337 5415 5434 6056
Size: 481 81 44 301 9 78 19 622
Cuts at: 6056 6475 6618 6619 6651 6668 6697 6698 6783
Size: 419 143 1 32 17 29 1 85
Cuts at: 6783 6791 7255 7515 7715 7859 8260 8808 9003
Size: 8 464 260 200 144 401 548 195
Cuts at: 9003 9142 9143 9288
Size: 139 1 145
Fragments arranged by size:
882 622 548 516 494 481 464 419
401 397 361 301 284 260 224 208
201 200 195 145 144 143 139 111
96 93 91 85 85 83 81 78
72 63 55 50 44 32 32 29
19 17 12 9 9 8 1 1
1 1 1
EcoRI G'AATT_C
Cuts at: 0 7834 8512 9288
Size: 7834 678 776
Fragments arranged by size:
7834 776 678
EcoRV GAT'ATC
Cuts at: 0 5543 9288
Size: 5543 3745
FseI GG_CCGG'CC
Cuts at: 0 55 823 9288
Size: 55 768 8465
Fragments arranged by size:
8465 768 55
FspI TGC'GCA
Cuts at: 0 6394 7468 9288
Size: 6394 1074 1820
Fragments arranged by size:
6394 1820 1074
HaeII r_GCGC'y
Cuts at: 0 25 33 327 604 860 1052 1952 2113
Size: 25 8 294 277 256 192 900 161
Cuts at: 2113 3351 4191 4458 4928 5005 5125 5233 5609
Size: 1238 840 267 470 77 120 108 376
Cuts at: 5609 5830 6763 7411 7728 8328 8729 9288
Size: 221 933 648 317 600 401 559
Fragments arranged by size:
1238 933 900 840 648 600 559 470
401 376 317 294 277 267 256 221
192 161 120 108 77 25 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 61 87 93 553 585 706 738
Size: 55 6 26 6 460 32 121 32
Cuts at: 738 1602 1634 2980 3012 3400 3432 3543 3575
Size: 864 32 1346 32 388 32 111 32
Cuts at: 3575 3748 3780 4251 4283 5155 5187 5911 5943
Size: 173 32 471 32 872 32 724 32
Cuts at: 5943 5992 6024 6988 7020 7702 7734 9005 9037
Size: 49 32 964 32 682 32 1271 32
Cuts at: 9037 9049 9081 9288
Size: 12 32 207
Fragments arranged by size:
1346 1271 964 872 864 724 682 471
460 388 207 173 121 111 55 49
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 26
12 6 6
HincII GTy'rAC
Cuts at: 0 4206 8216 9162 9288
Size: 4206 4010 946 126
Fragments arranged by size:
4206 4010 946 126
HindIII A'AGCT_T
Cuts at: 0 3889 6515 9288
Size: 3889 2626 2773
Fragments arranged by size:
3889 2773 2626
KasI G'GCGC_C
Cuts at: 0 323 856 1048 1948 2109 5229 5605 8324
Size: 323 533 192 900 161 3120 376 2719
Cuts at: 8324 9288
Size: 964
Fragments arranged by size:
3120 2719 964 900 533 376 323 192
161
KpnI G_GTAC'C
Cuts at: 0 1193 3064 4302 9288
Size: 1193 1871 1238 4986
Fragments arranged by size:
4986 1871 1238 1193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 743 891 1856 7706 9288
Size: 143 600 148 965 5850 1582
Fragments arranged by size:
5850 1582 965 600 148 143
MscI TGG'CCA
Cuts at: 0 2582 3119 3175 3713 4012 4818 6359 6556
Size: 2582 537 56 538 299 806 1541 197
Cuts at: 6556 6719 7540 9288
Size: 163 821 1748
Fragments arranged by size:
2582 1748 1541 821 806 538 537 299
197 163 56
MslI CAynn'nnrTG
Cuts at: 0 2447 3069 3274 3566 5701 5746 6454 7126
Size: 2447 622 205 292 2135 45 708 672
Cuts at: 7126 9052 9288
Size: 1926 236
Fragments arranged by size:
2447 2135 1926 708 672 622 292 236
205 45
MspA1I CmG'CkG
Cuts at: 0 125 317 732 852 937 999 1277 1331
Size: 125 192 415 120 85 62 278 54
Cuts at: 1331 2961 3684 3729 4161 4329 4686 4848 5046
Size: 1630 723 45 432 168 357 162 198
Cuts at: 5046 5085 5193 5790 5895 6343 6631 7206 7278
Size: 39 108 597 105 448 288 575 72
Cuts at: 7278 7371 7677 8547 8835 8952 9108 9175 9288
Size: 93 306 870 288 117 156 67 113
Fragments arranged by size:
1630 870 723 597 575 448 432 415
357 306 288 288 278 198 192 168
162 156 125 120 117 113 108 105
93 85 72 67 62 54 45 39
NaeI GCC'GGC
Cuts at: 0 53 821 7616 8329 8476 8620 9288
Size: 53 768 6795 713 147 144 668
Fragments arranged by size:
6795 768 713 668 147 144 53
NarI GG'CG_CC
Cuts at: 0 324 857 1049 1949 2110 5230 5606 8325
Size: 324 533 192 900 161 3120 376 2719
Cuts at: 8325 9288
Size: 963
Fragments arranged by size:
3120 2719 963 900 533 376 324 192
161
NcoI C'CATG_G
Cuts at: 0 3564 5636 5741 6354 9288
Size: 3564 2072 105 613 2934
Fragments arranged by size:
3564 2934 2072 613 105
NgoMIV G'CCGG_C
Cuts at: 0 51 819 7614 8327 8474 8618 9288
Size: 51 768 6795 713 147 144 670
Fragments arranged by size:
6795 768 713 670 147 144 51
NheI G'CTAG_C
Cuts at: 0 8814 9288
Size: 8814 474
NotI GC'GGCC_GC
Cuts at: 0 312 366 1165 9288
Size: 312 54 799 8123
Fragments arranged by size:
8123 799 312 54
NruI TCG'CGA
Cuts at: 0 1033 3129 9288
Size: 1033 2096 6159
Fragments arranged by size:
6159 2096 1033
NsiI A_TGCA'T
Cuts at: 0 2425 9288
Size: 2425 6863
NspI r_CATG'y
Cuts at: 0 3919 4275 6459 9000 9288
Size: 3919 356 2184 2541 288
Fragments arranged by size:
3919 2541 2184 356 288
PasI CC'CwG_GG
Cuts at: 0 238 305 687 1119 4026 4988 6165 6787
Size: 238 67 382 432 2907 962 1177 622
Cuts at: 6787 9288
Size: 2501
Fragments arranged by size:
2907 2501 1177 962 622 432 382 238
67
PciI A'CATG_T
Cuts at: 0 3915 9288
Size: 3915 5373
PflMI CCAn_nnn'nTGG
Cuts at: 0 33 244 1065 3006 3732 4026 4092 4200
Size: 33 211 821 1941 726 294 66 108
Cuts at: 4200 4851 6105 8274 9288
Size: 651 1254 2169 1014
Fragments arranged by size:
2169 1941 1254 1014 821 726 651 294
211 108 66 33
PfoI T'CCnGG_A
Cuts at: 0 775 3497 3746 4175 5516 7943 9288
Size: 775 2722 249 429 1341 2427 1345
Fragments arranged by size:
2722 2427 1345 1341 775 429 249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 8842 8874 9288
Size: 8842 32 414
Fragments arranged by size:
8842 414 32
PpuMI rG'GwC_Cy
Cuts at: 0 779 987 1563 1936 2021 2076 2169 2415
Size: 779 208 576 373 85 55 93 246
Cuts at: 2415 3822 4134 5328 5337 5434 6783 6791 7515
Size: 1407 312 1194 9 97 1349 8 724
Cuts at: 7515 7859 8808 9288
Size: 344 949 480
Fragments arranged by size:
1407 1349 1194 949 779 724 576 480
373 344 312 246 208 97 93 85
55 9 8
PshAI GACnn'nnGTC
Cuts at: 0 725 1319 9288
Size: 725 594 7969
Fragments arranged by size:
7969 725 594
PspOMI G'GGCC_C
Cuts at: 0 301 397 682 1068 4901 5066 5614 6474
Size: 301 96 285 386 3833 165 548 860
Cuts at: 6474 6618 6667 6697 9142 9288
Size: 144 49 30 2445 146
Fragments arranged by size:
3833 2445 860 548 386 301 285 165
146 144 96 49 30
PspXI vC'TCGA_Gb
Cuts at: 0 723 8168 9288
Size: 723 7445 1120
Fragments arranged by size:
7445 1120 723
PstI C_TGCA'G
Cuts at: 0 213 232 1994 2449 2798 2842 2897 2960
Size: 213 19 1762 455 349 44 55 63
Cuts at: 2960 4145 4385 5213 7492 8252 8564 8768 9107
Size: 1185 240 828 2279 760 312 204 339
Cuts at: 9107 9288
Size: 181
Fragments arranged by size:
2279 1762 1185 828 760 455 349 339
312 240 213 204 181 63 55 44
19
PvuII CAG'CTG
Cuts at: 0 2961 3684 3729 4329 4686 4848 5046 5085
Size: 2961 723 45 600 357 162 198 39
Cuts at: 5085 5193 5790 6631 7206 7278 7371 7677 8547
Size: 108 597 841 575 72 93 306 870
Cuts at: 8547 8835 8952 9175 9288
Size: 288 117 223 113
Fragments arranged by size:
2961 870 841 723 600 597 575 357
306 288 223 198 162 117 113 108
93 72 45 39
SacI G_AGCT'C
Cuts at: 0 4175 4838 9288
Size: 4175 663 4450
Fragments arranged by size:
4450 4175 663
SacII CC_GC'GG
Cuts at: 0 126 318 1278 1332 9288
Size: 126 192 960 54 7956
Fragments arranged by size:
7956 960 192 126 54
SalI G'TCGA_C
Cuts at: 0 4204 8214 9288
Size: 4204 4010 1074
Fragments arranged by size:
4204 4010 1074
SanDI GG'GwC_CC
Cuts at: 0 2021 5337 6791 9288
Size: 2021 3316 1454 2497
Fragments arranged by size:
3316 2497 2021 1454
SapI GCTCTTCn'nnn_
Cuts at: 0 350 3364 3544 7354 8533 9091 9288
Size: 350 3014 180 3810 1179 558 197
Fragments arranged by size:
3810 3014 1179 558 350 197 180
SbfI CC_TGCA'GG
Cuts at: 0 2798 2842 9288
Size: 2798 44 6446
Fragments arranged by size:
6446 2798 44
SexAI A'CCwGG_T
Cuts at: 0 905 3647 4793 9288
Size: 905 2742 1146 4495
Fragments arranged by size:
4495 2742 1146 905
SfcI C'TryA_G
Cuts at: 0 209 228 1990 2445 2644 2794 2838 2893
Size: 209 19 1762 455 199 150 44 55
Cuts at: 2893 2956 3352 4141 4381 4948 5209 6658 7488
Size: 63 396 789 240 567 261 1449 830
Cuts at: 7488 7988 8248 8560 8679 8764 9103 9288
Size: 500 260 312 119 85 339 185
Fragments arranged by size:
1762 1449 830 789 567 500 455 396
339 312 261 260 240 209 199 185
150 119 85 63 55 44 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 48 886 4009 5056 5065 9288
Size: 48 838 3123 1047 9 4223
Fragments arranged by size:
4223 3123 1047 838 48 9
SfoI GGC'GCC
Cuts at: 0 325 858 1050 1950 2111 5231 5607 8326
Size: 325 533 192 900 161 3120 376 2719
Cuts at: 8326 9288
Size: 962
Fragments arranged by size:
3120 2719 962 900 533 376 325 192
161
SgrAI Cr'CCGG_yG
Cuts at: 0 8327 9288
Size: 8327 961
SmaI CCC'GGG
Cuts at: 0 45 532 655 892 1473 2009 5239 6473
Size: 45 487 123 237 581 536 3230 1234
Cuts at: 6473 7390 8041 9288
Size: 917 651 1247
Fragments arranged by size:
3230 1247 1234 917 651 581 536 487
237 123 45
SmlI C'TyrA_G
Cuts at: 0 723 1087 2218 2689 4963 7240 7435 8168
Size: 723 364 1131 471 2274 2277 195 733
Cuts at: 8168 9288
Size: 1120
Fragments arranged by size:
2277 2274 1131 1120 733 723 471 364
195
SphI G_CATG'C
Cuts at: 0 4275 9000 9288
Size: 4275 4725 288
Fragments arranged by size:
4725 4275 288
SrfI GCCC'GGGC
Cuts at: 0 892 1473 6473 7390 9288
Size: 892 581 5000 917 1898
Fragments arranged by size:
5000 1898 917 892 581
StuI AGG'CCT
Cuts at: 0 880 2836 3899 4003 5222 5935 6507 7867
Size: 880 1956 1063 104 1219 713 572 1360
Cuts at: 7867 9288
Size: 1421
Fragments arranged by size:
1956 1421 1360 1219 1063 880 713 572
104
StyI C'CwwG_G
Cuts at: 0 824 1939 1976 2129 3176 3564 4119 4130
Size: 824 1115 37 153 1047 388 555 11
Cuts at: 4130 4424 5030 5636 5741 6149 6332 6347 6354
Size: 294 606 606 105 408 183 15 7
Cuts at: 6354 6360 6372 6539 6647 7251 7643 7738 7931
Size: 6 12 167 108 604 392 95 193
Cuts at: 7931 8729 9288
Size: 798 559
Fragments arranged by size:
1115 1047 824 798 606 606 604 559
555 408 392 388 294 193 183 167
153 108 105 95 37 15 12 11
7 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3411 5189 5709 6865 7306 8011 8689 9288
Size: 3411 1778 520 1156 441 705 678 599
Fragments arranged by size:
3411 1778 1156 705 678 599 520 441
TatI w'GTAC_w
Cuts at: 0 7925 9077 9288
Size: 7925 1152 211
Fragments arranged by size:
7925 1152 211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 905 937 2300 2332 2657 2689 3310 3342
Size: 905 32 1363 32 325 32 621 32
Cuts at: 3342 3382 3414 4928 4960 7920 7952 8455 8487
Size: 40 32 1514 32 2960 32 503 32
Cuts at: 8487 9288
Size: 801
Fragments arranged by size:
2960 1514 1363 905 801 621 503 325
40 32 32 32 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 7108 9288
Size: 7108 2180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 184 2958 3168 3228 3562 4344 5739 7407
Size: 184 2774 210 60 334 782 1395 1668
Cuts at: 7407 8271 9288
Size: 864 1017
Fragments arranged by size:
2774 1668 1395 1017 864 782 334 210
184 60
XhoI C'TCGA_G
Cuts at: 0 723 8168 9288
Size: 723 7445 1120
Fragments arranged by size:
7445 1120 723
XmaI C'CCGG_G
Cuts at: 0 43 530 653 890 1471 2007 5237 6471
Size: 43 487 123 237 581 536 3230 1234
Cuts at: 6471 7388 8039 9288
Size: 917 651 1249
Fragments arranged by size:
3230 1249 1234 917 651 581 536 487
237 123 43
XmnI GAAnn'nnTTC
Cuts at: 0 6094 6910 8359 9288
Size: 6094 816 1449 929
Fragments arranged by size:
6094 1449 929 816
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApoI AscI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI
FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PasI PciI PflMI PfoI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI
SacII SalI SanDI SapI SbfI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AhdI ApaLI AseI AsiSI BaeI BsaBI
BsiWI BspHI BsrGI BstBI BstZ17I ClaI CspCI FalI
FspAI HpaI MfeI MluI NdeI PacI PmeI PmlI
PsiI PsrI PvuI RsrII ScaI SgfI SnaBI SpeI
SspI SwaI XbaI ZraI