(Linear) (Six-base) MAPSORT of: KIAA1785.seq Check: 4726 from: 1 to: 1851
KIAA1785.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AccI GT'mk_AC
Cuts at: 0 767 1851
Size: 767 1084
Acc65I G'GTAC_C
Cuts at: 0 1496 1851
Size: 1496 355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1431 1851
Size: 307 1124 420
Fragments arranged by size:
1124 420 307
AflII C'TTAA_G
Cuts at: 0 1417 1851
Size: 1417 434
AflIII A'CryG_T
Cuts at: 0 1483 1851
Size: 1483 368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 209 581 1619 1781 1851
Size: 209 372 1038 162 70
Fragments arranged by size:
1038 372 209 162 70
AlwNI CAG_nnn'CTG
Cuts at: 0 574 1051 1851
Size: 574 477 800
Fragments arranged by size:
800 574 477
ApaLI G'TGCA_C
Cuts at: 0 1652 1851
Size: 1652 199
ApoI r'AATT_y
Cuts at: 0 172 1056 1510 1725 1851
Size: 172 884 454 215 126
Fragments arranged by size:
884 454 215 172 126
AseI AT'TA_AT
Cuts at: 0 734 970 1851
Size: 734 236 881
Fragments arranged by size:
881 734 236
AvaI C'yCGr_G
Cuts at: 0 32 1851
Size: 32 1819
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1199 1232 1851
Size: 1199 33 619
Fragments arranged by size:
1199 619 33
BamHI G'GATC_C
Cuts at: 0 1116 1851
Size: 1116 735
BanI G'GyrC_C
Cuts at: 0 155 1496 1851
Size: 155 1341 355
Fragments arranged by size:
1341 355 155
BanII G_rGCy'C
Cuts at: 0 217 252 1851
Size: 217 35 1599
Fragments arranged by size:
1599 217 35
BfrBI ATG'CAT
Cuts at: 0 1070 1851
Size: 1070 781
BglII A'GATC_T
Cuts at: 0 780 1851
Size: 780 1071
Bme1580I G_kGCm'C
Cuts at: 0 158 1656 1851
Size: 158 1498 195
Fragments arranged by size:
1498 195 158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 111 1851
Size: 111 1740
BsaAI yAC'GTr
Cuts at: 0 452 1851
Size: 452 1399
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 308 338 1851
Size: 308 30 1513
Fragments arranged by size:
1513 308 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 344 1851
Size: 100 244 1507
Fragments arranged by size:
1507 244 100
BseYI C'CCAG_C
Cuts at: 0 1203 1851
Size: 1203 648
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 932 1851
Size: 587 345 919
Fragments arranged by size:
919 587 345
BsiHKAI G_wGCw'C
Cuts at: 0 1656 1851
Size: 1656 195
BsmI GAATG_Cn'
Cuts at: 0 962 1851
Size: 962 889
Bsp1286I G_dGCh'C
Cuts at: 0 158 217 252 1656 1851
Size: 158 59 35 1404 195
Fragments arranged by size:
1404 195 158 59 35
BspHI T'CATG_A
Cuts at: 0 590 1628 1851
Size: 590 1038 223
Fragments arranged by size:
1038 590 223
BsrDI GCAATG_nn'
Cuts at: 0 193 553 821 1753 1851
Size: 193 360 268 932 98
Fragments arranged by size:
932 360 268 193 98
BssSI C'ACGA_G
Cuts at: 0 1772 1851
Size: 1772 79
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1244 1851
Size: 1244 607
BstXI CCAn_nnnn'nTGG
Cuts at: 0 66 1851
Size: 66 1785
BstYI r'GATC_y
Cuts at: 0 3 780 1116 1851
Size: 3 777 336 735
Fragments arranged by size:
777 735 336 3
BtsI GCAGTG_nn'
Cuts at: 0 198 1765 1851
Size: 198 1567 86
Fragments arranged by size:
1567 198 86
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 668 703 1851
Size: 668 35 1148
Fragments arranged by size:
1148 668 35
EaeI y'GGCC_r
Cuts at: 0 140 378 1851
Size: 140 238 1473
Fragments arranged by size:
1473 238 140
EarI CTCTTCn'nnn_
Cuts at: 0 362 911 1460 1851
Size: 362 549 549 391
Fragments arranged by size:
549 549 391 362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1431 1851
Size: 307 1124 420
Fragments arranged by size:
1124 420 307
EcoO109I rG'GnC_Cy
Cuts at: 0 1320 1851
Size: 1320 531
EcoRI G'AATT_C
Cuts at: 0 172 1851
Size: 172 1679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1461 1851
Size: 1461 390
HaeII r_GCGC'y
Cuts at: 0 1037 1851
Size: 1037 814
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 308 340 1022 1054 1851
Size: 308 32 682 32 797
Fragments arranged by size:
797 682 308 32 32
HincII GTy'rAC
Cuts at: 0 327 1851
Size: 327 1524
KpnI G_GTAC'C
Cuts at: 0 1500 1851
Size: 1500 351
MscI TGG'CCA
Cuts at: 0 380 1851
Size: 380 1471
MslI CAynn'nnrTG
Cuts at: 0 563 595 1661 1758 1851
Size: 563 32 1066 97 93
Fragments arranged by size:
1066 563 97 93 32
MspA1I CmG'CkG
Cuts at: 0 1297 1851
Size: 1297 554
NsiI A_TGCA'T
Cuts at: 0 1072 1851
Size: 1072 779
NspI r_CATG'y
Cuts at: 0 1487 1851
Size: 1487 364
PciI A'CATG_T
Cuts at: 0 1483 1851
Size: 1483 368
PshAI GACnn'nnGTC
Cuts at: 0 1586 1851
Size: 1586 265
PsiI TTA'TAA
Cuts at: 0 1802 1851
Size: 1802 49
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 811 843 1851
Size: 811 32 1008
Fragments arranged by size:
1008 811 32
PstI C_TGCA'G
Cuts at: 0 276 1851
Size: 276 1575
PvuII CAG'CTG
Cuts at: 0 1297 1851
Size: 1297 554
SapI GCTCTTCn'nnn_
Cuts at: 0 911 1851
Size: 911 940
SfcI C'TryA_G
Cuts at: 0 272 1180 1851
Size: 272 908 671
Fragments arranged by size:
908 671 272
SmlI C'TyrA_G
Cuts at: 0 1417 1851
Size: 1417 434
SspI AAT'ATT
Cuts at: 0 684 846 1851
Size: 684 162 1005
Fragments arranged by size:
1005 684 162
StuI AGG'CCT
Cuts at: 0 1199 1851
Size: 1199 652
StyI C'CwwG_G
Cuts at: 0 896 1429 1851
Size: 896 533 422
Fragments arranged by size:
896 533 422
TatI w'GTAC_w
Cuts at: 0 1369 1851
Size: 1369 482
XbaI T'CTAG_A
Cuts at: 0 741 1374 1851
Size: 741 633 477
Fragments arranged by size:
741 633 477
XmnI GAAnn'nnTTC
Cuts at: 0 983 1444 1851
Size: 983 461 407
Fragments arranged by size:
983 461 407
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AlfI AlwNI ApaLI
ApoI AseI AvaI BaeI BamHI BanI BanII BfrBI
BglII Bme1580I BplI BsaAI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BsrDI BssSI BstAPI BstXI
BstYI BtsI CspCI EaeI EarI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HincII KpnI MscI MslI MspA1I
NsiI NspI PciI PshAI PsiI PsrI PstI PvuII
SapI SfcI SmlI SspI StuI StyI TatI XbaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AleI AloI
ApaI AscI AsiSI AvrII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BlpI BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoRV FseI FspI
FspAI HindIII HpaI KasI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PspXI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI TaqII TstI Tth111I XcmI
XhoI XmaI ZraI