(Linear) (Six-base) MAPSORT of: KIAA1785.seq Check: 4726 from: 1 to: 1851 KIAA1785.orf With 182 enzymes: SgfI * October 24, 2006 15:17 .. AccI GT'mk_AC Cuts at: 0 767 1851 Size: 767 1084 Acc65I G'GTAC_C Cuts at: 0 1496 1851 Size: 1496 355 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 1431 1851 Size: 307 1124 420 Fragments arranged by size: 1124 420 307 AflII C'TTAA_G Cuts at: 0 1417 1851 Size: 1417 434 AflIII A'CryG_T Cuts at: 0 1483 1851 Size: 1483 368 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 209 581 1619 1781 1851 Size: 209 372 1038 162 70 Fragments arranged by size: 1038 372 209 162 70 AlwNI CAG_nnn'CTG Cuts at: 0 574 1051 1851 Size: 574 477 800 Fragments arranged by size: 800 574 477 ApaLI G'TGCA_C Cuts at: 0 1652 1851 Size: 1652 199 ApoI r'AATT_y Cuts at: 0 172 1056 1510 1725 1851 Size: 172 884 454 215 126 Fragments arranged by size: 884 454 215 172 126 AseI AT'TA_AT Cuts at: 0 734 970 1851 Size: 734 236 881 Fragments arranged by size: 881 734 236 AvaI C'yCGr_G Cuts at: 0 32 1851 Size: 32 1819 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1199 1232 1851 Size: 1199 33 619 Fragments arranged by size: 1199 619 33 BamHI G'GATC_C Cuts at: 0 1116 1851 Size: 1116 735 BanI G'GyrC_C Cuts at: 0 155 1496 1851 Size: 155 1341 355 Fragments arranged by size: 1341 355 155 BanII G_rGCy'C Cuts at: 0 217 252 1851 Size: 217 35 1599 Fragments arranged by size: 1599 217 35 BfrBI ATG'CAT Cuts at: 0 1070 1851 Size: 1070 781 BglII A'GATC_T Cuts at: 0 780 1851 Size: 780 1071 Bme1580I G_kGCm'C Cuts at: 0 158 1656 1851 Size: 158 1498 195 Fragments arranged by size: 1498 195 158 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 111 1851 Size: 111 1740 BsaAI yAC'GTr Cuts at: 0 452 1851 Size: 452 1399 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 308 338 1851 Size: 308 30 1513 Fragments arranged by size: 1513 308 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 100 344 1851 Size: 100 244 1507 Fragments arranged by size: 1507 244 100 BseYI C'CCAG_C Cuts at: 0 1203 1851 Size: 1203 648 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 587 932 1851 Size: 587 345 919 Fragments arranged by size: 919 587 345 BsiHKAI G_wGCw'C Cuts at: 0 1656 1851 Size: 1656 195 BsmI GAATG_Cn' Cuts at: 0 962 1851 Size: 962 889 Bsp1286I G_dGCh'C Cuts at: 0 158 217 252 1656 1851 Size: 158 59 35 1404 195 Fragments arranged by size: 1404 195 158 59 35 BspHI T'CATG_A Cuts at: 0 590 1628 1851 Size: 590 1038 223 Fragments arranged by size: 1038 590 223 BsrDI GCAATG_nn' Cuts at: 0 193 553 821 1753 1851 Size: 193 360 268 932 98 Fragments arranged by size: 932 360 268 193 98 BssSI C'ACGA_G Cuts at: 0 1772 1851 Size: 1772 79 BstAPI GCAn_nnn'nTGC Cuts at: 0 1244 1851 Size: 1244 607 BstXI CCAn_nnnn'nTGG Cuts at: 0 66 1851 Size: 66 1785 BstYI r'GATC_y Cuts at: 0 3 780 1116 1851 Size: 3 777 336 735 Fragments arranged by size: 777 735 336 3 BtsI GCAGTG_nn' Cuts at: 0 198 1765 1851 Size: 198 1567 86 Fragments arranged by size: 1567 198 86 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 668 703 1851 Size: 668 35 1148 Fragments arranged by size: 1148 668 35 EaeI y'GGCC_r Cuts at: 0 140 378 1851 Size: 140 238 1473 Fragments arranged by size: 1473 238 140 EarI CTCTTCn'nnn_ Cuts at: 0 362 911 1460 1851 Size: 362 549 549 391 Fragments arranged by size: 549 549 391 362 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 1431 1851 Size: 307 1124 420 Fragments arranged by size: 1124 420 307 EcoO109I rG'GnC_Cy Cuts at: 0 1320 1851 Size: 1320 531 EcoRI G'AATT_C Cuts at: 0 172 1851 Size: 172 1679 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1461 1851 Size: 1461 390 HaeII r_GCGC'y Cuts at: 0 1037 1851 Size: 1037 814 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 308 340 1022 1054 1851 Size: 308 32 682 32 797 Fragments arranged by size: 797 682 308 32 32 HincII GTy'rAC Cuts at: 0 327 1851 Size: 327 1524 KpnI G_GTAC'C Cuts at: 0 1500 1851 Size: 1500 351 MscI TGG'CCA Cuts at: 0 380 1851 Size: 380 1471 MslI CAynn'nnrTG Cuts at: 0 563 595 1661 1758 1851 Size: 563 32 1066 97 93 Fragments arranged by size: 1066 563 97 93 32 MspA1I CmG'CkG Cuts at: 0 1297 1851 Size: 1297 554 NsiI A_TGCA'T Cuts at: 0 1072 1851 Size: 1072 779 NspI r_CATG'y Cuts at: 0 1487 1851 Size: 1487 364 PciI A'CATG_T Cuts at: 0 1483 1851 Size: 1483 368 PshAI GACnn'nnGTC Cuts at: 0 1586 1851 Size: 1586 265 PsiI TTA'TAA Cuts at: 0 1802 1851 Size: 1802 49 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 811 843 1851 Size: 811 32 1008 Fragments arranged by size: 1008 811 32 PstI C_TGCA'G Cuts at: 0 276 1851 Size: 276 1575 PvuII CAG'CTG Cuts at: 0 1297 1851 Size: 1297 554 SapI GCTCTTCn'nnn_ Cuts at: 0 911 1851 Size: 911 940 SfcI C'TryA_G Cuts at: 0 272 1180 1851 Size: 272 908 671 Fragments arranged by size: 908 671 272 SmlI C'TyrA_G Cuts at: 0 1417 1851 Size: 1417 434 SspI AAT'ATT Cuts at: 0 684 846 1851 Size: 684 162 1005 Fragments arranged by size: 1005 684 162 StuI AGG'CCT Cuts at: 0 1199 1851 Size: 1199 652 StyI C'CwwG_G Cuts at: 0 896 1429 1851 Size: 896 533 422 Fragments arranged by size: 896 533 422 TatI w'GTAC_w Cuts at: 0 1369 1851 Size: 1369 482 XbaI T'CTAG_A Cuts at: 0 741 1374 1851 Size: 741 633 477 Fragments arranged by size: 741 633 477 XmnI GAAnn'nnTTC Cuts at: 0 983 1444 1851 Size: 983 461 407 Fragments arranged by size: 983 461 407 Enzymes that do cut: AccI Acc65I AcuI AflII AflIII AlfI AlwNI ApaLI ApoI AseI AvaI BaeI BamHI BanI BanII BfrBI BglII Bme1580I BplI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BssSI BstAPI BstXI BstYI BtsI CspCI EaeI EarI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII KpnI MscI MslI MspA1I NsiI NspI PciI PshAI PsiI PsrI PstI PvuII SapI SfcI SmlI SspI StuI StyI TatI XbaI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AgeI AhdI AleI AloI ApaI AscI AsiSI AvrII BbeI BbsI BbvCI BcgI BciVI BclI BglI BlpI BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TaqII TstI Tth111I XcmI XhoI XmaI ZraI