Restriction map for
pF1KSDA1787
(Linear) (Six-base) MAPSORT of: KIAA1787.seq Check: 9594 from: 1 to: 4686
KIAA1787.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3513 3592 3852 3993 4686
Size: 3513 79 260 141 693
Fragments arranged by size:
3513 693 260 141 79
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 615 704 2378 2817 3743 4593 4686
Size: 388 227 89 1674 439 926 850 93
Fragments arranged by size:
1674 926 850 439 388 227 93 89
AfeI AGC'GCT
Cuts at: 0 323 4686
Size: 323 4363
AflIII A'CryG_T
Cuts at: 0 252 3200 3713 4686
Size: 252 2948 513 973
Fragments arranged by size:
2948 973 513 252
AhdI GACnn_n'nnGTC
Cuts at: 0 2316 4686
Size: 2316 2370
AleI CACnn'nnGTG
Cuts at: 0 2047 3457 4686
Size: 2047 1410 1229
Fragments arranged by size:
2047 1410 1229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1693 4686
Size: 1693 2993
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 862 894 4686
Size: 862 32 3792
Fragments arranged by size:
3792 862 32
AlwNI CAG_nnn'CTG
Cuts at: 0 447 496 1552 2077 2728 3184 3516 3892
Size: 447 49 1056 525 651 456 332 376
Cuts at: 3892 4321 4686
Size: 429 365
Fragments arranged by size:
1056 651 525 456 447 429 376 365
332 49
ApaI G_GGCC'C
Cuts at: 0 73 1975 2265 3704 4686
Size: 73 1902 290 1439 982
Fragments arranged by size:
1902 1439 982 290 73
ApaLI G'TGCA_C
Cuts at: 0 594 2492 4394 4686
Size: 594 1898 1902 292
Fragments arranged by size:
1902 1898 594 292
ApoI r'AATT_y
Cuts at: 0 1033 1270 2368 4686
Size: 1033 237 1098 2318
Fragments arranged by size:
2318 1098 1033 237
AseI AT'TA_AT
Cuts at: 0 1349 4686
Size: 1349 3337
AvaI C'yCGr_G
Cuts at: 0 664 1507 1995 2571 2953 3394 4160 4403
Size: 664 843 488 576 382 441 766 243
Cuts at: 4403 4563 4686
Size: 160 123
Fragments arranged by size:
843 766 664 576 488 441 382 243
160 123
BanI G'GyrC_C
Cuts at: 0 1204 1249 3280 3504 3727 4686
Size: 1204 45 2031 224 223 959
Fragments arranged by size:
2031 1204 959 224 223 45
BanII G_rGCy'C
Cuts at: 0 31 73 379 671 860 880 1677 1975
Size: 31 42 306 292 189 20 797 298
Cuts at: 1975 2143 2265 3002 3704 4686
Size: 168 122 737 702 982
Fragments arranged by size:
982 797 737 702 306 298 292 189
168 122 42 31 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 249 692 2366 2823 3016 3657 4686
Size: 249 443 1674 457 193 641 1029
Fragments arranged by size:
1674 1029 641 457 443 249 193
BbvCI CC'TCA_GC
Cuts at: 0 2848 2855 4686
Size: 2848 7 1831
Fragments arranged by size:
2848 1831 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2781 2815 4000 4034 4076 4110 4375 4409
Size: 2781 34 1185 34 42 34 265 34
Cuts at: 4409 4686
Size: 277
Fragments arranged by size:
2781 1185 277 265 42 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3036 4686
Size: 3036 1650
BglI GCCn_nnn'nGGC
Cuts at: 0 1543 3359 3597 3725 4686
Size: 1543 1816 238 128 961
Fragments arranged by size:
1816 1543 961 238 128
BglII A'GATC_T
Cuts at: 0 3683 4686
Size: 3683 1003
BlpI GC'TnA_GC
Cuts at: 0 1010 1663 4686
Size: 1010 653 3023
Fragments arranged by size:
3023 1010 653
Bme1580I G_kGCm'C
Cuts at: 0 73 598 1252 1880 1975 1994 2265 2488
Size: 73 525 654 628 95 19 271 223
Cuts at: 2488 2496 3509 3627 3704 3721 3818 4398 4668
Size: 8 1013 118 77 17 97 580 270
Cuts at: 4668 4686
Size: 18
Fragments arranged by size:
1013 654 628 580 525 271 270 223
118 97 95 77 73 19 18 17
8
BmgBI CAC'GTC
Cuts at: 0 2058 4686
Size: 2058 2628
BmrI ACTGGGnnnn_n'
Cuts at: 0 152 326 1384 1838 2716 3293 4686
Size: 152 174 1058 454 878 577 1393
Fragments arranged by size:
1393 1058 878 577 454 174 152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 1180 1762 2054 2357 3043 3286 3424
Size: 312 868 582 292 303 686 243 138
Cuts at: 3424 4240 4686
Size: 816 446
Fragments arranged by size:
868 816 686 582 446 312 303 292
243 138
Bpu10I CC'TnA_GC
Cuts at: 0 87 620 874 1553 2848 2855 3881 4333
Size: 87 533 254 679 1295 7 1026 452
Cuts at: 4333 4520 4686
Size: 187 166
Fragments arranged by size:
1295 1026 679 533 452 254 187 166
87 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1879 3664 4645 4686
Size: 799 1080 1785 981 41
Fragments arranged by size:
1785 1080 981 799 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 3682 4456 4686
Size: 3682 774 230
Fragments arranged by size:
3682 774 230
BsaAI yAC'GTr
Cuts at: 0 3716 4686
Size: 3716 970
BsaBI GATnn'nnATC
Cuts at: 0 1096 3145 4686
Size: 1096 2049 1541
Fragments arranged by size:
2049 1541 1096
BsaHI Gr'CG_yC
Cuts at: 0 1384 2000 2240 4686
Size: 1384 616 240 2446
Fragments arranged by size:
2446 1384 616 240
BsaWI w'CCGG_w
Cuts at: 0 3002 4686
Size: 3002 1684
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 862 892 3273 3303 4686
Size: 862 30 2381 30 1383
Fragments arranged by size:
2381 1383 862 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 445 2794 3283 3340 3358 4139 4390 4391
Size: 445 2349 489 57 18 781 251 1
Cuts at: 4391 4423 4648 4686
Size: 32 225 38
Fragments arranged by size:
2349 781 489 445 251 225 57 38
32 18 1
BseYI C'CCAG_C
Cuts at: 0 73 496 741 1176 2481 2916 3093 3284
Size: 73 423 245 435 1305 435 177 191
Cuts at: 3284 3509 3818 3971 4225 4308 4453 4502 4686
Size: 225 309 153 254 83 145 49 184
Fragments arranged by size:
1305 435 435 423 309 254 245 225
191 184 177 153 145 83 73 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 2149 2515 2698 3541 4209 4686
Size: 115 2034 366 183 843 668 477
Fragments arranged by size:
2034 843 668 477 366 183 115
BsiEI CG_ry'CG
Cuts at: 0 276 757 4686
Size: 276 481 3929
Fragments arranged by size:
3929 481 276
BsiHKAI G_wGCw'C
Cuts at: 0 598 880 1518 2496 3002 4398 4686
Size: 598 282 638 978 506 1396 288
Fragments arranged by size:
1396 978 638 598 506 288 282
BsiWI C'GTAC_G
Cuts at: 0 175 3445 4686
Size: 175 3270 1241
Fragments arranged by size:
3270 1241 175
BsmBI CGTCTCn'nnnn_
Cuts at: 0 754 1474 4121 4686
Size: 754 720 2647 565
Fragments arranged by size:
2647 754 720 565
Bsp1286I G_dGCh'C
Cuts at: 0 31 73 379 598 671 860 880 1252
Size: 31 42 306 219 73 189 20 372
Cuts at: 1252 1518 1677 1880 1975 1994 2143 2265 2488
Size: 266 159 203 95 19 149 122 223
Cuts at: 2488 2496 3002 3509 3627 3704 3721 3818 4398
Size: 8 506 507 118 77 17 97 580
Cuts at: 4398 4668 4686
Size: 270 18
Fragments arranged by size:
580 507 506 372 306 270 266 223
219 203 189 159 149 122 118 97
95 77 73 42 31 20 19 18
17 8
BspEI T'CCGG_A
Cuts at: 0 3002 4686
Size: 3002 1684
BspHI T'CATG_A
Cuts at: 0 960 1052 1211 4686
Size: 960 92 159 3475
Fragments arranged by size:
3475 960 159 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2156 2537 2893 3513 3592 3852 3993 4181
Size: 2156 381 356 620 79 260 141 188
Cuts at: 4181 4306 4686
Size: 125 380
Fragments arranged by size:
2156 620 381 380 356 260 188 141
125 79
BsrBI CCG'CTC
Cuts at: 0 83 296 1220 4686
Size: 83 213 924 3466
Fragments arranged by size:
3466 924 213 83
BsrDI GCAATG_nn'
Cuts at: 0 3188 4275 4686
Size: 3188 1087 411
Fragments arranged by size:
3188 1087 411
BsrFI r'CCGG_y
Cuts at: 0 885 1016 2653 3310 4686
Size: 885 131 1637 657 1376
Fragments arranged by size:
1637 1376 885 657 131
BssHII G'CGCG_C
Cuts at: 0 3587 4686
Size: 3587 1099
BssSI C'ACGA_G
Cuts at: 0 378 560 1603 2790 3258 4686
Size: 378 182 1043 1187 468 1428
Fragments arranged by size:
1428 1187 1043 468 378 182
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2803 4321 4686
Size: 2803 1518 365
Fragments arranged by size:
2803 1518 365
BstEII G'GTnAC_C
Cuts at: 0 1143 1297 1434 4686
Size: 1143 154 137 3252
Fragments arranged by size:
3252 1143 154 137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 339 1167 2049 3021 3264 3459 4686
Size: 339 828 882 972 243 195 1227
Fragments arranged by size:
1227 972 882 828 339 243 195
BstYI r'GATC_y
Cuts at: 0 904 1091 3216 3683 4686
Size: 904 187 2125 467 1003
Fragments arranged by size:
2125 1003 904 467 187
Bsu36I CC'TnA_GG
Cuts at: 0 1621 2090 2127 4686
Size: 1621 469 37 2559
Fragments arranged by size:
2559 1621 469 37
BtgI C'CryG_G
Cuts at: 0 213 359 2660 3234 3411 3657 4686
Size: 213 146 2301 574 177 246 1029
Fragments arranged by size:
2301 1029 574 246 213 177 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 260 4686
Size: 260 4426
BtsI GCAGTG_nn'
Cuts at: 0 360 1433 1613 2687 2710 2804 3321 3859
Size: 360 1073 180 1074 23 94 517 538
Cuts at: 3859 4171 4686
Size: 312 515
Fragments arranged by size:
1074 1073 538 517 515 360 312 180
94 23
ClaI AT'CG_AT
Cuts at: 0 1358 4686
Size: 1358 3328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2737 2772 3171 3206 4686
Size: 2737 35 399 35 1480
Fragments arranged by size:
2737 1480 399 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1656 4686
Size: 1656 3030
DrdI GACnn_nn'nnGTC
Cuts at: 0 280 2245 4686
Size: 280 1965 2441
Fragments arranged by size:
2441 1965 280
EaeI y'GGCC_r
Cuts at: 0 754 2025 2547 2619 3075 3449 3461 4686
Size: 754 1271 522 72 456 374 12 1225
Fragments arranged by size:
1271 1225 754 522 456 374 72 12
EagI C'GGCC_G
Cuts at: 0 754 4686
Size: 754 3932
EarI CTCTTCn'nnn_
Cuts at: 0 1570 2876 4686
Size: 1570 1306 1810
Fragments arranged by size:
1810 1570 1306
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1462 1510 2200 4067 4474 4686
Size: 1462 48 690 1867 407 212
Fragments arranged by size:
1867 1462 690 407 212 48
EcoICRI GAG'CTC
Cuts at: 0 878 3000 4686
Size: 878 2122 1686
Fragments arranged by size:
2122 1686 878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 388 615 704 1180 1762 2054 2357
Size: 312 76 227 89 476 582 292 303
Cuts at: 2357 2378 2817 3043 3286 3424 3743 4240 4593
Size: 21 439 226 243 138 319 497 353
Cuts at: 4593 4686
Size: 93
Fragments arranged by size:
582 497 476 439 353 319 312 303
292 243 227 226 138 93 89 76
21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 623 3884 4364 4686
Size: 623 3261 480 322
Fragments arranged by size:
3261 623 480 322
EcoO109I rG'GnC_Cy
Cuts at: 0 40 69 70 444 710 974 1625 1814
Size: 40 29 1 374 266 264 651 189
Cuts at: 1814 1971 1972 2262 2950 3172 4491 4567 4686
Size: 157 1 290 688 222 1319 76 119
Fragments arranged by size:
1319 688 651 374 290 266 264 222
189 157 119 76 40 29 1 1
EcoRI G'AATT_C
Cuts at: 0 1033 1270 2368 4686
Size: 1033 237 1098 2318
Fragments arranged by size:
2318 1098 1033 237
FspI TGC'GCA
Cuts at: 0 1705 2452 4686
Size: 1705 747 2234
Fragments arranged by size:
2234 1705 747
FspAI rTGC'GCAy
Cuts at: 0 1705 4686
Size: 1705 2981
HaeII r_GCGC'y
Cuts at: 0 150 325 1017 1501 1560 2564 4686
Size: 150 175 692 484 59 1004 2122
Fragments arranged by size:
2122 1004 692 484 175 150 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 403 435 1105 1137 2302 2334 4326 4358
Size: 403 32 670 32 1165 32 1992 32
Cuts at: 4358 4483 4515 4686
Size: 125 32 171
Fragments arranged by size:
1992 1165 670 403 171 125 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 285 4686
Size: 285 4401
HindIII A'AGCT_T
Cuts at: 0 1105 4686
Size: 1105 3581
MfeI C'AATT_G
Cuts at: 0 3245 4686
Size: 3245 1441
MluI A'CGCG_T
Cuts at: 0 252 4686
Size: 252 4434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 407 965 1349 2279 3194 4686
Size: 125 282 558 384 930 915 1492
Fragments arranged by size:
1492 930 915 558 384 282 125
MscI TGG'CCA
Cuts at: 0 2027 2621 3463 4686
Size: 2027 594 842 1223
Fragments arranged by size:
2027 1223 842 594
MslI CAynn'nnrTG
Cuts at: 0 1210 1213 2047 2407 2503 3019 3151 3457
Size: 1210 3 834 360 96 516 132 306
Cuts at: 3457 3773 3779 4686
Size: 316 6 907
Fragments arranged by size:
1210 907 834 516 360 316 306 132
96 6 3
MspA1I CmG'CkG
Cuts at: 0 77 119 2163 2315 2809 3032 3236 3513
Size: 77 42 2044 152 494 223 204 277
Cuts at: 3513 4279 4506 4579 4686
Size: 766 227 73 107
Fragments arranged by size:
2044 766 494 277 227 223 204 152
107 77 73 42
NaeI GCC'GGC
Cuts at: 0 1018 4686
Size: 1018 3668
NcoI C'CATG_G
Cuts at: 0 2660 3411 4686
Size: 2660 751 1275
Fragments arranged by size:
2660 1275 751
NgoMIV G'CCGG_C
Cuts at: 0 1016 4686
Size: 1016 3670
PasI CC'CwG_GG
Cuts at: 0 2974 4686
Size: 2974 1712
PflMI CCAn_nnn'nTGG
Cuts at: 0 219 2728 3501 3516 4244 4686
Size: 219 2509 773 15 728 442
Fragments arranged by size:
2509 773 728 442 219 15
PfoI T'CCnGG_A
Cuts at: 0 289 1862 3401 4487 4686
Size: 289 1573 1539 1086 199
Fragments arranged by size:
1573 1539 1086 289 199
PmlI CAC'GTG
Cuts at: 0 3716 4686
Size: 3716 970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 862 894 4686
Size: 862 32 3792
Fragments arranged by size:
3792 862 32
PpuMI rG'GwC_Cy
Cuts at: 0 444 710 974 1814 2950 4491 4567 4686
Size: 444 266 264 840 1136 1541 76 119
Fragments arranged by size:
1541 1136 840 444 266 264 119 76
PshAI GACnn'nnGTC
Cuts at: 0 96 1441 4686
Size: 96 1345 3245
Fragments arranged by size:
3245 1345 96
PspOMI G'GGCC_C
Cuts at: 0 69 1971 2261 3700 4686
Size: 69 1902 290 1439 986
Fragments arranged by size:
1902 1439 986 290 69
PspXI vC'TCGA_Gb
Cuts at: 0 664 3394 4686
Size: 664 2730 1292
Fragments arranged by size:
2730 1292 664
PstI C_TGCA'G
Cuts at: 0 720 3180 4686
Size: 720 2460 1506
Fragments arranged by size:
2460 1506 720
PvuII CAG'CTG
Cuts at: 0 2163 3032 3513 4686
Size: 2163 869 481 1173
Fragments arranged by size:
2163 1173 869 481
SacI G_AGCT'C
Cuts at: 0 880 3002 4686
Size: 880 2122 1684
Fragments arranged by size:
2122 1684 880
SacII CC_GC'GG
Cuts at: 0 3237 4686
Size: 3237 1449
SanDI GG'GwC_CC
Cuts at: 0 974 2950 4491 4567 4686
Size: 974 1976 1541 76 119
Fragments arranged by size:
1976 1541 974 119 76
SapI GCTCTTCn'nnn_
Cuts at: 0 1570 2876 4686
Size: 1570 1306 1810
Fragments arranged by size:
1810 1570 1306
ScaI AGT'ACT
Cuts at: 0 1264 2754 4686
Size: 1264 1490 1932
Fragments arranged by size:
1932 1490 1264
SexAI A'CCwGG_T
Cuts at: 0 2102 4686
Size: 2102 2584
SfcI C'TryA_G
Cuts at: 0 716 3176 4686
Size: 716 2460 1510
Fragments arranged by size:
2460 1510 716
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3359 4686
Size: 3359 1327
SgrAI Cr'CCGG_yG
Cuts at: 0 1016 4686
Size: 1016 3670
SmaI CCC'GGG
Cuts at: 0 1997 2955 4162 4565 4686
Size: 1997 958 1207 403 121
Fragments arranged by size:
1997 1207 958 403 121
SmlI C'TyrA_G
Cuts at: 0 664 778 1894 3394 3679 4624 4686
Size: 664 114 1116 1500 285 945 62
Fragments arranged by size:
1500 1116 945 664 285 114 62
StuI AGG'CCT
Cuts at: 0 551 899 4686
Size: 551 348 3787
Fragments arranged by size:
3787 551 348
StyI C'CwwG_G
Cuts at: 0 671 695 2519 2556 2660 2840 3411 3918
Size: 671 24 1824 37 104 180 571 507
Cuts at: 3918 4584 4686
Size: 666 102
Fragments arranged by size:
1824 671 666 571 507 180 104 102
37 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 504 613 1165 1615 1774 2453 3103 3298
Size: 504 109 552 450 159 679 650 195
Cuts at: 3298 4313 4686
Size: 1015 373
Fragments arranged by size:
1015 679 650 552 504 450 373 195
159 109
TatI w'GTAC_w
Cuts at: 0 1262 2752 4686
Size: 1262 1490 1934
Fragments arranged by size:
1934 1490 1262
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 407 439 2400 2432 3273 3305 4686
Size: 407 32 1961 32 841 32 1381
Fragments arranged by size:
1961 1381 841 407 32 32 32
XbaI T'CTAG_A
Cuts at: 0 2775 4686
Size: 2775 1911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 1576 2658 4686
Size: 108 1468 1082 2028
Fragments arranged by size:
2028 1468 1082 108
XhoI C'TCGA_G
Cuts at: 0 664 3394 4686
Size: 664 2730 1292
Fragments arranged by size:
2730 1292 664
XmaI C'CCGG_G
Cuts at: 0 1995 2953 4160 4563 4686
Size: 1995 958 1207 403 123
Fragments arranged by size:
1995 1207 958 403 123
XmnI GAAnn'nnTTC
Cuts at: 0 2704 4686
Size: 2704 1982
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AvaI BanI BanII
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SacII SanDI SapI ScaI SexAI
SfcI SfiI SgrAI SmaI SmlI StuI StyI TaqII
TatI TstI XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AscI AsiSI
AvrII BaeI BamHI BbeI BclI BfrBI BmtI BplI
BsmI BsrGI BstBI BstZ17I DraI EcoRV FalI FseI
HpaI KasI KpnI NarI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PsiI PsrI PvuI
RsrII SalI SbfI SfoI SgfI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I ZraI