Restriction map for
pF1KA1788
(Linear) (Six-base) MAPSORT of: KIAA1788.seq Check: 2717 from: 1 to: 1233
KIAA1788.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 283 1233
Size: 283 950
AflIII A'CryG_T
Cuts at: 0 719 1233
Size: 719 514
AlwNI CAG_nnn'CTG
Cuts at: 0 216 1233
Size: 216 1017
ApaI G_GGCC'C
Cuts at: 0 192 569 1233
Size: 192 377 664
Fragments arranged by size:
664 377 192
AvaI C'yCGr_G
Cuts at: 0 884 1170 1233
Size: 884 286 63
Fragments arranged by size:
884 286 63
BanI G'GyrC_C
Cuts at: 0 379 981 1233
Size: 379 602 252
Fragments arranged by size:
602 379 252
BanII G_rGCy'C
Cuts at: 0 91 192 256 478 569 1145 1233
Size: 91 101 64 222 91 576 88
Fragments arranged by size:
576 222 101 91 91 88 64
BbeI G_GCGC'C
Cuts at: 0 383 1233
Size: 383 850
BbsI GAAGACnn'nnnn_
Cuts at: 0 687 709 1233
Size: 687 22 524
Fragments arranged by size:
687 524 22
BbvCI CC'TCA_GC
Cuts at: 0 1117 1233
Size: 1117 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 365 399 823 857 1233
Size: 365 34 424 34 376
Fragments arranged by size:
424 376 365 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1133 1233
Size: 1133 100
Bme1580I G_kGCm'C
Cuts at: 0 192 569 1233
Size: 192 377 664
Fragments arranged by size:
664 377 192
BmgBI CAC'GTC
Cuts at: 0 720 1233
Size: 720 513
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 400 697 706 1233
Size: 250 150 297 9 527
Fragments arranged by size:
527 297 250 150 9
Bpu10I CC'TnA_GC
Cuts at: 0 1117 1233
Size: 1117 116
BsaAI yAC'GTr
Cuts at: 0 1074 1233
Size: 1074 159
BsaHI Gr'CG_yC
Cuts at: 0 53 176 380 391 1233
Size: 53 123 204 11 842
Fragments arranged by size:
842 204 123 53 11
BsaWI w'CCGG_w
Cuts at: 0 650 1233
Size: 650 583
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 1233
Size: 76 30 1127
Fragments arranged by size:
1127 76 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 694 1233
Size: 694 539
BseYI C'CCAG_C
Cuts at: 0 202 241 275 296 341 553 595 1233
Size: 202 39 34 21 45 212 42 638
Fragments arranged by size:
638 212 202 45 42 39 34 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1233
Size: 793 440
BsiEI CG_ry'CG
Cuts at: 0 41 149 1233
Size: 41 108 1084
Fragments arranged by size:
1084 108 41
BsiHKAI G_wGCw'C
Cuts at: 0 478 1145 1208 1233
Size: 478 667 63 25
Fragments arranged by size:
667 478 63 25
BsmI GAATG_Cn'
Cuts at: 0 9 1233
Size: 9 1224
BsmBI CGTCTCn'nnnn_
Cuts at: 0 24 1233
Size: 24 1209
Bsp1286I G_dGCh'C
Cuts at: 0 91 192 256 478 569 1145 1208 1233
Size: 91 101 64 222 91 576 63 25
Fragments arranged by size:
576 222 101 91 91 64 63 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 441 765 828 1233
Size: 441 324 63 405
Fragments arranged by size:
441 405 324 63
BsrFI r'CCGG_y
Cuts at: 0 36 1233
Size: 36 1197
BstBI TT'CG_AA
Cuts at: 0 843 1233
Size: 843 390
BstXI CCAn_nnnn'nTGG
Cuts at: 0 603 1015 1233
Size: 603 412 218
Fragments arranged by size:
603 412 218
BtgI C'CryG_G
Cuts at: 0 47 1233
Size: 47 1186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1161 1233
Size: 1161 72
BtsI GCAGTG_nn'
Cuts at: 0 857 1233
Size: 857 376
EaeI y'GGCC_r
Cuts at: 0 38 146 432 740 1214 1233
Size: 38 108 286 308 474 19
Fragments arranged by size:
474 308 286 108 38 19
EagI C'GGCC_G
Cuts at: 0 38 146 1233
Size: 38 108 1087
Fragments arranged by size:
1087 108 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 660 1233
Size: 660 573
EcoICRI GAG'CTC
Cuts at: 0 476 1143 1233
Size: 476 667 90
Fragments arranged by size:
667 476 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 400 697 706 1233
Size: 250 150 297 9 527
Fragments arranged by size:
527 297 250 150 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 760 1233
Size: 760 473
EcoO109I rG'GnC_Cy
Cuts at: 0 213 565 566 1233
Size: 213 352 1 667
Fragments arranged by size:
667 352 213 1
HaeII r_GCGC'y
Cuts at: 0 203 383 1233
Size: 203 180 850
Fragments arranged by size:
850 203 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 1233
Size: 76 32 1125
Fragments arranged by size:
1125 76 32
HincII GTy'rAC
Cuts at: 0 284 1233
Size: 284 949
KasI G'GCGC_C
Cuts at: 0 379 1233
Size: 379 854
MscI TGG'CCA
Cuts at: 0 742 1233
Size: 742 491
MspA1I CmG'CkG
Cuts at: 0 43 431 675 738 1233
Size: 43 388 244 63 495
Fragments arranged by size:
495 388 244 63 43
NaeI GCC'GGC
Cuts at: 0 38 1233
Size: 38 1195
NarI GG'CG_CC
Cuts at: 0 380 1233
Size: 380 853
NcoI C'CATG_G
Cuts at: 0 47 1233
Size: 47 1186
NdeI CA'TA_TG
Cuts at: 0 864 1233
Size: 864 369
NgoMIV G'CCGG_C
Cuts at: 0 36 1233
Size: 36 1197
NspI r_CATG'y
Cuts at: 0 996 1233
Size: 996 237
PasI CC'CwG_GG
Cuts at: 0 480 1233
Size: 480 753
PflMI CCAn_nnn'nTGG
Cuts at: 0 1223 1233
Size: 1223 10
PfoI T'CCnGG_A
Cuts at: 0 111 416 1233
Size: 111 305 817
Fragments arranged by size:
817 305 111
PmlI CAC'GTG
Cuts at: 0 1074 1233
Size: 1074 159
PpuMI rG'GwC_Cy
Cuts at: 0 213 1233
Size: 213 1020
PshAI GACnn'nnGTC
Cuts at: 0 16 1233
Size: 16 1217
PspOMI G'GGCC_C
Cuts at: 0 188 565 1233
Size: 188 377 668
Fragments arranged by size:
668 377 188
PspXI vC'TCGA_Gb
Cuts at: 0 1170 1233
Size: 1170 63
PvuII CAG'CTG
Cuts at: 0 675 738 1233
Size: 675 63 495
Fragments arranged by size:
675 495 63
RsrII CG'GwC_CG
Cuts at: 0 1158 1233
Size: 1158 75
SacI G_AGCT'C
Cuts at: 0 478 1145 1233
Size: 478 667 88
Fragments arranged by size:
667 478 88
SalI G'TCGA_C
Cuts at: 0 282 1233
Size: 282 951
SfcI C'TryA_G
Cuts at: 0 60 1233
Size: 60 1173
SfoI GGC'GCC
Cuts at: 0 381 1233
Size: 381 852
SmlI C'TyrA_G
Cuts at: 0 1170 1233
Size: 1170 63
StuI AGG'CCT
Cuts at: 0 1127 1233
Size: 1127 106
StyI C'CwwG_G
Cuts at: 0 47 1199 1233
Size: 47 1152 34
Fragments arranged by size:
1152 47 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 472 1233
Size: 472 761
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 672 1233
Size: 672 561
XhoI C'TCGA_G
Cuts at: 0 1170 1233
Size: 1170 63
XmnI GAAnn'nnTTC
Cuts at: 0 694 1233
Size: 694 539
Enzymes that do cut:
AccI AflIII AlwNI ApaI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BglI Bme1580I BmgBI BpmI Bpu10I
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BstBI BstXI
BtgI BtgZI BtsI EaeI EagI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MscI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PvuII RsrII
SacI SalI SfcI SfoI SmlI StuI StyI TaqII
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BplI BpuEI BsaI BsaBI BsiWI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EarI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI MslI NheI
NotI NruI NsiI PacI PciI PmeI PpiI PsiI
PsrI PstI PvuI SacII SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XmaI
ZraI