Restriction map for
pF1KA1793
(Linear) (Six-base) MAPSORT of: KIAA1793.seq Check: 1265 from: 1 to: 3462
KIAA1793.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2055 2485 3462
Size: 2055 430 977
Fragments arranged by size:
2055 977 430
AatII G_ACGT'C
Cuts at: 0 835 3457 3462
Size: 835 2622 5
Fragments arranged by size:
2622 835 5
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 938 1120 1168 1426 2549 2692 3462
Size: 836 102 182 48 258 1123 143 770
Fragments arranged by size:
1123 836 770 258 182 143 102 48
AfeI AGC'GCT
Cuts at: 0 2542 3462
Size: 2542 920
AflII C'TTAA_G
Cuts at: 0 406 3462
Size: 406 3056
AflIII A'CryG_T
Cuts at: 0 1297 1499 3462
Size: 1297 202 1963
Fragments arranged by size:
1963 1297 202
AlwNI CAG_nnn'CTG
Cuts at: 0 195 456 684 1112 1272 1335 2061 2501
Size: 195 261 228 428 160 63 726 440
Cuts at: 2501 3205 3462
Size: 704 257
Fragments arranged by size:
726 704 440 428 261 257 228 195
160 63
ApaI G_GGCC'C
Cuts at: 0 726 3462
Size: 726 2736
AvaI C'yCGr_G
Cuts at: 0 224 2933 3462
Size: 224 2709 529
Fragments arranged by size:
2709 529 224
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2279 2312 3462
Size: 2279 33 1150
Fragments arranged by size:
2279 1150 33
BanII G_rGCy'C
Cuts at: 0 189 275 622 726 1034 1163 1196 1556
Size: 189 86 347 104 308 129 33 360
Cuts at: 1556 1700 2102 2891 3462
Size: 144 402 789 571
Fragments arranged by size:
789 571 402 360 347 308 189 144
129 104 86 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 554 1832 2537 3281 3462
Size: 554 1278 705 744 181
Fragments arranged by size:
1278 744 705 554 181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2500 2534 3462
Size: 2500 34 928
Fragments arranged by size:
2500 928 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1377 3006 3462
Size: 1377 1629 456
Fragments arranged by size:
1629 1377 456
BclI T'GATC_A
Cuts at: 0 2570 3462
Size: 2570 892
BfrBI ATG'CAT
Cuts at: 0 930 3462
Size: 930 2532
BglI GCCn_nnn'nGGC
Cuts at: 0 307 2086 2392 3462
Size: 307 1779 306 1070
Fragments arranged by size:
1779 1070 307 306
BglII A'GATC_T
Cuts at: 0 765 3462
Size: 765 2697
BlpI GC'TnA_GC
Cuts at: 0 1436 2522 3191 3462
Size: 1436 1086 669 271
Fragments arranged by size:
1436 1086 669 271
Bme1580I G_kGCm'C
Cuts at: 0 726 3462
Size: 726 2736
BmtI G_CTAG'C
Cuts at: 0 295 2816 3462
Size: 295 2521 646
Fragments arranged by size:
2521 646 295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 270 606 3462
Size: 270 336 2856
Fragments arranged by size:
2856 336 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 235 298 790 1264 1504 1534 1561
Size: 193 42 63 492 474 240 30 27
Cuts at: 1561 1723 2878 3070 3462
Size: 162 1155 192 392
Fragments arranged by size:
1155 492 474 392 240 193 192 162
63 42 30 27
Bpu10I CC'TnA_GC
Cuts at: 0 188 2899 3462
Size: 188 2711 563
Fragments arranged by size:
2711 563 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1540 1780 2242 2344 3052 3462
Size: 1018 522 240 462 102 708 410
Fragments arranged by size:
1018 708 522 462 410 240 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 649 3028 3462
Size: 214 435 2379 434
Fragments arranged by size:
2379 435 434 214
BsaBI GATnn'nnATC
Cuts at: 0 583 3462
Size: 583 2879
BsaHI Gr'CG_yC
Cuts at: 0 832 2078 3299 3454 3462
Size: 832 1246 1221 155 8
Fragments arranged by size:
1246 1221 832 155 8
BsaWI w'CCGG_w
Cuts at: 0 631 1575 3462
Size: 631 944 1887
Fragments arranged by size:
1887 944 631
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 257 287 1021 1051 1087 1117 1240 1270
Size: 257 30 734 30 36 30 123 30
Cuts at: 1270 1336 1366 3462
Size: 66 30 2096
Fragments arranged by size:
2096 734 257 123 66 36 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 79 190 232 382 1018 1021 1108
Size: 58 21 111 42 150 636 3 87
Cuts at: 1108 1357 1728 1741 2146 2899 3121 3136 3145
Size: 249 371 13 405 753 222 15 9
Cuts at: 3145 3277 3301 3304 3462
Size: 132 24 3 158
Fragments arranged by size:
753 636 405 371 249 222 158 150
132 111 87 58 42 24 21 15
13 9 3 3
BseYI C'CCAG_C
Cuts at: 0 30 302 2257 3462
Size: 30 272 1955 1205
Fragments arranged by size:
1955 1205 272 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 620 1258 1738 2397 2487 2608 2866
Size: 121 499 638 480 659 90 121 258
Cuts at: 2866 3462
Size: 596
Fragments arranged by size:
659 638 596 499 480 258 121 121
90
BsiHKAI G_wGCw'C
Cuts at: 0 622 1034 1044 1163 1196 1556 1700 2102
Size: 622 412 10 119 33 360 144 402
Cuts at: 2102 2891 3462
Size: 789 571
Fragments arranged by size:
789 622 571 412 402 360 144 119
33 10
BsmI GAATG_Cn'
Cuts at: 0 560 2725 2949 2973 3462
Size: 560 2165 224 24 489
Fragments arranged by size:
2165 560 489 224 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1420 3445 3462
Size: 1420 2025 17
Fragments arranged by size:
2025 1420 17
Bsp1286I G_dGCh'C
Cuts at: 0 189 275 622 726 1034 1044 1163 1196
Size: 189 86 347 104 308 10 119 33
Cuts at: 1196 1556 1700 2102 2891 3462
Size: 360 144 402 789 571
Fragments arranged by size:
789 571 402 360 347 308 189 144
119 104 86 33 10
BspEI T'CCGG_A
Cuts at: 0 1575 3462
Size: 1575 1887
BspHI T'CATG_A
Cuts at: 0 1211 3462
Size: 1211 2251
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2055 2485 2526 3462
Size: 2055 430 41 936
Fragments arranged by size:
2055 936 430 41
BsrBI CCG'CTC
Cuts at: 0 1488 2136 3462
Size: 1488 648 1326
Fragments arranged by size:
1488 1326 648
BsrDI GCAATG_nn'
Cuts at: 0 1416 2268 3462
Size: 1416 852 1194
Fragments arranged by size:
1416 1194 852
BsrFI r'CCGG_y
Cuts at: 0 1259 2080 3462
Size: 1259 821 1382
Fragments arranged by size:
1382 1259 821
BstAPI GCAn_nnn'nTGC
Cuts at: 0 684 1272 1335 2061 2586 2720 3462
Size: 684 588 63 726 525 134 742
Fragments arranged by size:
742 726 684 588 525 134 63
BstEII G'GTnAC_C
Cuts at: 0 2994 3462
Size: 2994 468
BstYI r'GATC_y
Cuts at: 0 329 446 519 765 1922 3201 3462
Size: 329 117 73 246 1157 1279 261
Fragments arranged by size:
1279 1157 329 261 246 117 73
Bsu36I CC'TnA_GG
Cuts at: 0 1378 3206 3462
Size: 1378 1828 256
Fragments arranged by size:
1828 1378 256
BtgI C'CryG_G
Cuts at: 0 2735 3462
Size: 2735 727
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 973 3462
Size: 973 2489
BtsI GCAGTG_nn'
Cuts at: 0 120 1357 1612 1696 1765 1771 2878 3175
Size: 120 1237 255 84 69 6 1107 297
Cuts at: 3175 3462
Size: 287
Fragments arranged by size:
1237 1107 297 287 255 120 84 69
6
EaeI y'GGCC_r
Cuts at: 0 2654 3462
Size: 2654 808
EarI CTCTTCn'nnn_
Cuts at: 0 44 50 56 68 610 914 968 1742
Size: 44 6 6 12 542 304 54 774
Cuts at: 1742 1925 2134 2230 2398 2440 2479 2672 2902
Size: 183 209 96 168 42 39 193 230
Cuts at: 2902 3239 3254 3278 3312 3462
Size: 337 15 24 34 150
Fragments arranged by size:
774 542 337 304 230 209 193 183
168 150 96 54 44 42 39 34
24 15 12 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2881 3462
Size: 2881 581
EcoICRI GAG'CTC
Cuts at: 0 620 1032 1161 1194 1554 1698 2100 2889
Size: 620 412 129 33 360 144 402 789
Cuts at: 2889 3462
Size: 573
Fragments arranged by size:
789 620 573 412 402 360 144 129
33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 235 298 790 836 938 1120 1168
Size: 193 42 63 492 46 102 182 48
Cuts at: 1168 1264 1426 1504 1534 1561 1723 2549 2692
Size: 96 162 78 30 27 162 826 143
Cuts at: 2692 2878 3070 3462
Size: 186 192 392
Fragments arranged by size:
826 492 392 193 192 186 182 162
162 143 102 96 78 63 48 46
42 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 514 709 2694 3404 3462
Size: 514 195 1985 710 58
Fragments arranged by size:
1985 710 514 195 58
EcoO109I rG'GnC_Cy
Cuts at: 0 723 1521 2058 3462
Size: 723 798 537 1404
Fragments arranged by size:
1404 798 723 537
HaeII r_GCGC'y
Cuts at: 0 302 2544 3462
Size: 302 2242 918
Fragments arranged by size:
2242 918 302
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 140 172 438 470 490 522 1087 1119
Size: 140 32 266 32 20 32 565 32
Cuts at: 1119 1419 1451 3289 3321 3442 3462
Size: 300 32 1838 32 121 20
Fragments arranged by size:
1838 565 300 266 140 121 32 32
32 32 32 32 20 20
HincII GTy'rAC
Cuts at: 0 135 3462
Size: 135 3327
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 1952 3462
Size: 950 1002 1510
Fragments arranged by size:
1510 1002 950
MscI TGG'CCA
Cuts at: 0 2656 3462
Size: 2656 806
MslI CAynn'nnrTG
Cuts at: 0 248 494 1502 3462
Size: 248 246 1008 1960
Fragments arranged by size:
1960 1008 248 246
MspA1I CmG'CkG
Cuts at: 0 201 1176 1335 1458 1512 1539 1677 1740
Size: 201 975 159 123 54 27 138 63
Cuts at: 1740 1845 2184 2501 2628 2799 2856 2868 3462
Size: 105 339 317 127 171 57 12 594
Fragments arranged by size:
975 594 339 317 201 171 159 138
127 123 105 63 57 54 27 12
NaeI GCC'GGC
Cuts at: 0 1261 3462
Size: 1261 2201
NcoI C'CATG_G
Cuts at: 0 2735 3462
Size: 2735 727
NgoMIV G'CCGG_C
Cuts at: 0 1259 3462
Size: 1259 2203
NheI G'CTAG_C
Cuts at: 0 291 2812 3462
Size: 291 2521 650
Fragments arranged by size:
2521 650 291
NsiI A_TGCA'T
Cuts at: 0 932 3462
Size: 932 2530
NspI r_CATG'y
Cuts at: 0 1301 1503 1863 3462
Size: 1301 202 360 1599
Fragments arranged by size:
1599 1301 360 202
PciI A'CATG_T
Cuts at: 0 1297 1499 3462
Size: 1297 202 1963
Fragments arranged by size:
1963 1297 202
PflMI CCAn_nnn'nTGG
Cuts at: 0 1783 3462
Size: 1783 1679
PfoI T'CCnGG_A
Cuts at: 0 275 1700 1961 3462
Size: 275 1425 261 1501
Fragments arranged by size:
1501 1425 275 261
PpuMI rG'GwC_Cy
Cuts at: 0 1521 3462
Size: 1521 1941
PshAI GACnn'nnGTC
Cuts at: 0 2081 3462
Size: 2081 1381
PspOMI G'GGCC_C
Cuts at: 0 722 3462
Size: 722 2740
PstI C_TGCA'G
Cuts at: 0 200 515 680 731 812 1025 1124 1268
Size: 200 315 165 51 81 213 99 144
Cuts at: 1268 1340 1352 1373 1682 1745 1844 2066 2150
Size: 72 12 21 309 63 99 222 84
Cuts at: 2150 2582 2777 2798 2873 3462
Size: 432 195 21 75 589
Fragments arranged by size:
589 432 315 309 222 213 200 195
165 144 99 99 84 81 75 72
63 51 21 21 12
PvuII CAG'CTG
Cuts at: 0 201 1176 1335 1458 1512 1539 1677 1740
Size: 201 975 159 123 54 27 138 63
Cuts at: 1740 1845 2184 2501 2628 2799 2856 2868 3462
Size: 105 339 317 127 171 57 12 594
Fragments arranged by size:
975 594 339 317 201 171 159 138
127 123 105 63 57 54 27 12
SacI G_AGCT'C
Cuts at: 0 622 1034 1163 1196 1556 1700 2102 2891
Size: 622 412 129 33 360 144 402 789
Cuts at: 2891 3462
Size: 571
Fragments arranged by size:
789 622 571 412 402 360 144 129
33
SapI GCTCTTCn'nnn_
Cuts at: 0 610 914 968 1742 1925 2230 2672 3462
Size: 610 304 54 774 183 305 442 790
Fragments arranged by size:
790 774 610 442 305 304 183 54
SbfI CC_TGCA'GG
Cuts at: 0 515 731 2066 3462
Size: 515 216 1335 1396
Fragments arranged by size:
1396 1335 515 216
SfcI C'TryA_G
Cuts at: 0 196 265 511 676 727 808 979 1021
Size: 196 69 246 165 51 81 171 42
Cuts at: 1021 1120 1264 1336 1348 1369 1678 1741 1840
Size: 99 144 72 12 21 309 63 99
Cuts at: 1840 2062 2146 2578 2773 2794 2869 3462
Size: 222 84 432 195 21 75 593
Fragments arranged by size:
593 432 309 246 222 196 195 171
165 144 99 99 84 81 75 72
69 63 51 42 21 21 12
SmaI CCC'GGG
Cuts at: 0 226 2935 3462
Size: 226 2709 527
Fragments arranged by size:
2709 527 226
SmlI C'TyrA_G
Cuts at: 0 406 1033 1555 1795 2221 2359 3031 3462
Size: 406 627 522 240 426 138 672 431
Fragments arranged by size:
672 627 522 431 426 406 240 138
SphI G_CATG'C
Cuts at: 0 1863 3462
Size: 1863 1599
StuI AGG'CCT
Cuts at: 0 1561 2395 2913 3410 3462
Size: 1561 834 518 497 52
Fragments arranged by size:
1561 834 518 497 52
StyI C'CwwG_G
Cuts at: 0 257 342 1854 2735 3462
Size: 257 85 1512 881 727
Fragments arranged by size:
1512 881 727 257 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1099 2350 3462
Size: 1099 1251 1112
Fragments arranged by size:
1251 1112 1099
TatI w'GTAC_w
Cuts at: 0 1646 3062 3462
Size: 1646 1416 400
Fragments arranged by size:
1646 1416 400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 1240 1272 1336 1368 3462
Size: 1021 32 187 32 64 32 2094
Fragments arranged by size:
2094 1021 187 64 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3456 3462
Size: 3456 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2040 3045 3462
Size: 2040 1005 417
Fragments arranged by size:
2040 1005 417
XmaI C'CCGG_G
Cuts at: 0 224 2933 3462
Size: 224 2709 529
Fragments arranged by size:
2709 529 224
XmnI GAAnn'nnTTC
Cuts at: 0 560 3462
Size: 560 2902
ZraI GAC'GTC
Cuts at: 0 833 3455 3462
Size: 833 2622 7
Fragments arranged by size:
2622 833 7
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AflIII AlwNI ApaI
AvaI BaeI BanII BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstEII BstYI Bsu36I BtgI
BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NheI NsiI NspI PciI PflMI
PfoI PpuMI PshAI PspOMI PstI PvuII SacI SapI
SbfI SfcI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AgeI AhdI AleI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BamHI BanI
BbeI BbvCI BmgBI BmrI BsaAI BsiEI BsiWI BsrGI
BssHII BssSI BstBI BstXI BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NotI NruI PacI PasI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XbaI XhoI