Restriction map for
pF1KA1805
(Linear) (Six-base) MAPSORT of: KIAA1805.seq Check: 5513 from: 1 to: 1701
KIAA1805.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AccI GT'mk_AC
Cuts at: 0 1040 1247 1701
Size: 1040 207 454
Fragments arranged by size:
1040 454 207
Acc65I G'GTAC_C
Cuts at: 0 1113 1701
Size: 1113 588
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1146 1701
Size: 1087 59 555
Fragments arranged by size:
1087 555 59
AflII C'TTAA_G
Cuts at: 0 153 1701
Size: 153 1548
AleI CACnn'nnGTG
Cuts at: 0 872 1701
Size: 872 829
AlwNI CAG_nnn'CTG
Cuts at: 0 1056 1110 1701
Size: 1056 54 591
Fragments arranged by size:
1056 591 54
ApoI r'AATT_y
Cuts at: 0 343 1342 1701
Size: 343 999 359
Fragments arranged by size:
999 359 343
AvaI C'yCGr_G
Cuts at: 0 1543 1701
Size: 1543 158
BanI G'GyrC_C
Cuts at: 0 1113 1701
Size: 1113 588
BanII G_rGCy'C
Cuts at: 0 1154 1285 1701
Size: 1154 131 416
Fragments arranged by size:
1154 416 131
BbsI GAAGACnn'nnnn_
Cuts at: 0 338 544 1195 1318 1701
Size: 338 206 651 123 383
Fragments arranged by size:
651 383 338 206 123
BbvCI CC'TCA_GC
Cuts at: 0 1391 1701
Size: 1391 310
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 54 88 1701
Size: 54 34 1613
Fragments arranged by size:
1613 54 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 93 751 1701
Size: 93 658 950
Fragments arranged by size:
950 658 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 789 1077 1701
Size: 789 288 624
Fragments arranged by size:
789 624 288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 759 1701
Size: 759 942
Bpu10I CC'TnA_GC
Cuts at: 0 751 1391 1701
Size: 751 640 310
Fragments arranged by size:
751 640 310
BsaI GGTCTCn'nnnn_
Cuts at: 0 1511 1701
Size: 1511 190
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 460 490 1363 1393 1701
Size: 460 30 873 30 308
Fragments arranged by size:
873 460 308 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 481 1596 1701
Size: 75 406 1115 105
Fragments arranged by size:
1115 406 105 75
BsiHKAI G_wGCw'C
Cuts at: 0 67 106 1701
Size: 67 39 1595
Fragments arranged by size:
1595 67 39
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1011 1701
Size: 1011 690
Bsp1286I G_dGCh'C
Cuts at: 0 67 106 1154 1285 1701
Size: 67 39 1048 131 416
Fragments arranged by size:
1048 416 131 67 39
BspHI T'CATG_A
Cuts at: 0 141 1701
Size: 141 1560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 526 1701
Size: 526 1175
BsrDI GCAATG_nn'
Cuts at: 0 478 1701
Size: 478 1223
BsrGI T'GTAC_A
Cuts at: 0 1288 1701
Size: 1288 413
BssSI C'ACGA_G
Cuts at: 0 66 1701
Size: 66 1635
BstXI CCAn_nnnn'nTGG
Cuts at: 0 795 1701
Size: 795 906
BstZ17I GTA'TAC
Cuts at: 0 1041 1701
Size: 1041 660
Bsu36I CC'TnA_GG
Cuts at: 0 916 1701
Size: 916 785
BtsI GCAGTG_nn'
Cuts at: 0 507 533 1260 1701
Size: 507 26 727 441
Fragments arranged by size:
727 507 441 26
DraI TTT'AAA
Cuts at: 0 554 1701
Size: 554 1147
EaeI y'GGCC_r
Cuts at: 0 1073 1701
Size: 1073 628
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 759 1087 1146 1701
Size: 759 328 59 555
Fragments arranged by size:
759 555 328 59
EcoO109I rG'GnC_Cy
Cuts at: 0 37 95 1107 1701
Size: 37 58 1012 594
Fragments arranged by size:
1012 594 58 37
EcoRV GAT'ATC
Cuts at: 0 802 1197 1701
Size: 802 395 504
Fragments arranged by size:
802 504 395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 624 656 1701
Size: 624 32 1045
Fragments arranged by size:
1045 624 32
HindIII A'AGCT_T
Cuts at: 0 364 1428 1701
Size: 364 1064 273
Fragments arranged by size:
1064 364 273
KpnI G_GTAC'C
Cuts at: 0 1117 1701
Size: 1117 584
MscI TGG'CCA
Cuts at: 0 1075 1701
Size: 1075 626
MslI CAynn'nnrTG
Cuts at: 0 177 602 872 1701
Size: 177 425 270 829
Fragments arranged by size:
829 425 270 177
MspA1I CmG'CkG
Cuts at: 0 256 1458 1701
Size: 256 1202 243
Fragments arranged by size:
1202 256 243
NspI r_CATG'y
Cuts at: 0 762 1701
Size: 762 939
PflMI CCAn_nnn'nTGG
Cuts at: 0 528 1082 1701
Size: 528 554 619
Fragments arranged by size:
619 554 528
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1384 1416 1701
Size: 1384 32 285
Fragments arranged by size:
1384 285 32
PpuMI rG'GwC_Cy
Cuts at: 0 37 95 1107 1701
Size: 37 58 1012 594
Fragments arranged by size:
1012 594 58 37
PstI C_TGCA'G
Cuts at: 0 840 1701
Size: 840 861
PvuII CAG'CTG
Cuts at: 0 256 1701
Size: 256 1445
SanDI GG'GwC_CC
Cuts at: 0 37 1701
Size: 37 1664
ScaI AGT'ACT
Cuts at: 0 1175 1701
Size: 1175 526
SexAI A'CCwGG_T
Cuts at: 0 1109 1701
Size: 1109 592
SfcI C'TryA_G
Cuts at: 0 836 1048 1248 1701
Size: 836 212 200 453
Fragments arranged by size:
836 453 212 200
SmlI C'TyrA_G
Cuts at: 0 153 1701
Size: 153 1548
SphI G_CATG'C
Cuts at: 0 762 1701
Size: 762 939
StyI C'CwwG_G
Cuts at: 0 296 1076 1701
Size: 296 780 625
Fragments arranged by size:
780 625 296
TatI w'GTAC_w
Cuts at: 0 128 1173 1288 1701
Size: 128 1045 115 413
Fragments arranged by size:
1045 413 128 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1289 1321 1363 1395 1701
Size: 1289 32 42 32 306
Fragments arranged by size:
1289 306 42 32 32
Enzymes that do cut:
AccI Acc65I AcuI AflII AleI AlwNI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BmrI BplI BpmI
Bpu10I BsaI BsaXI BseRI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstXI BstZ17I Bsu36I BtsI
DraI EaeI Eco57MI EcoO109I EcoRV Hin4I HindIII KpnI
MscI MslI MspA1I NspI PflMI PpiI PpuMI PstI
PvuII SanDI ScaI SexAI SfcI SmlI SphI StyI
TatI TstI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmtI BpuEI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiWI BsmI BspEI BsrBI BsrFI
BssHII BstAPI BstBI BstEII BstYI BtgI BtgZI ClaI
CspCI DraIII DrdI EagI EarI EciI EcoICRI EcoNI
EcoRI FalI FseI FspI FspAI HaeII HincII HpaI
KasI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI