Restriction map for
pF1KSDA1809
(Linear) (Six-base) MAPSORT of: KIAA1809.seq Check: 2710 from: 1 to: 2319
KIAA1809.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AatII G_ACGT'C
Cuts at: 0 1637 2319
Size: 1637 682
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 1279 1656 2319
Size: 626 653 377 663
Fragments arranged by size:
663 653 626 377
AflII C'TTAA_G
Cuts at: 0 33 2311 2319
Size: 33 2278 8
Fragments arranged by size:
2278 33 8
AflIII A'CryG_T
Cuts at: 0 511 974 1235 1961 2319
Size: 511 463 261 726 358
Fragments arranged by size:
726 511 463 358 261
AleI CACnn'nnGTG
Cuts at: 0 881 2319
Size: 881 1438
AlwNI CAG_nnn'CTG
Cuts at: 0 224 1695 2319
Size: 224 1471 624
Fragments arranged by size:
1471 624 224
ApaI G_GGCC'C
Cuts at: 0 2188 2319
Size: 2188 131
ApaLI G'TGCA_C
Cuts at: 0 1180 1239 2319
Size: 1180 59 1080
Fragments arranged by size:
1180 1080 59
ApoI r'AATT_y
Cuts at: 0 46 356 1159 2319
Size: 46 310 803 1160
Fragments arranged by size:
1160 803 310 46
AscI GG'CGCG_CC
Cuts at: 0 1564 2319
Size: 1564 755
AvaI C'yCGr_G
Cuts at: 0 55 879 1824 1839 1912 2107 2130 2187
Size: 55 824 945 15 73 195 23 57
Cuts at: 2187 2301 2319
Size: 114 18
Fragments arranged by size:
945 824 195 114 73 57 55 23
18 15
BanI G'GyrC_C
Cuts at: 0 1351 2319
Size: 1351 968
BanII G_rGCy'C
Cuts at: 0 114 438 500 1891 2188 2271 2310 2319
Size: 114 324 62 1391 297 83 39 9
Fragments arranged by size:
1391 324 297 114 83 62 39 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 1279 1766 2319
Size: 1279 487 553
Fragments arranged by size:
1279 553 487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1893 1927 2319
Size: 1893 34 392
Fragments arranged by size:
1893 392 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 646 2319
Size: 646 1673
BglI GCCn_nnn'nGGC
Cuts at: 0 1199 2319
Size: 1199 1120
BlpI GC'TnA_GC
Cuts at: 0 1549 1654 1885 2319
Size: 1549 105 231 434
Fragments arranged by size:
1549 434 231 105
Bme1580I G_kGCm'C
Cuts at: 0 1184 1243 2188 2319
Size: 1184 59 945 131
Fragments arranged by size:
1184 945 131 59
BmgBI CAC'GTC
Cuts at: 0 977 2319
Size: 977 1342
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1599 1854 2088 2319
Size: 1599 255 234 231
Fragments arranged by size:
1599 255 234 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 420 1576 1980 2019 2319
Size: 98 322 1156 404 39 300
Fragments arranged by size:
1156 404 322 300 98 39
Bpu10I CC'TnA_GC
Cuts at: 0 2020 2319
Size: 2020 299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1372 2255 2319
Size: 1372 883 64
Fragments arranged by size:
1372 883 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 1327 2319
Size: 1327 992
BsaBI GATnn'nnATC
Cuts at: 0 738 2319
Size: 738 1581
BsaHI Gr'CG_yC
Cuts at: 0 1069 1634 2319
Size: 1069 565 685
Fragments arranged by size:
1069 685 565
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1309 1339 1948 1978 2008 2319
Size: 1309 30 609 30 30 311
Fragments arranged by size:
1309 609 311 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 402 1073 1091 1468 1477 1504 1513
Size: 124 278 671 18 377 9 27 9
Cuts at: 1513 1516 1519 1522 2046 2061 2319
Size: 3 3 3 524 15 258
Fragments arranged by size:
671 524 377 278 258 124 27 18
15 9 9 3 3 3
BseYI C'CCAG_C
Cuts at: 0 26 499 668 1006 1109 1866 1876 2197
Size: 26 473 169 338 103 757 10 321
Cuts at: 2197 2319
Size: 122
Fragments arranged by size:
757 473 338 321 169 122 103 26
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 410 482 674 2319
Size: 379 31 72 192 1645
Fragments arranged by size:
1645 379 192 72 31
BsiEI CG_ry'CG
Cuts at: 0 1598 1940 2319
Size: 1598 342 379
Fragments arranged by size:
1598 379 342
BsiHKAI G_wGCw'C
Cuts at: 0 114 197 438 1184 1243 1655 2069 2164
Size: 114 83 241 746 59 412 414 95
Cuts at: 2164 2271 2319
Size: 107 48
Fragments arranged by size:
746 414 412 241 114 107 95 83
59 48
Bsp1286I G_dGCh'C
Cuts at: 0 114 197 438 500 1184 1243 1655 1891
Size: 114 83 241 62 684 59 412 236
Cuts at: 1891 2069 2164 2188 2271 2310 2319
Size: 178 95 24 83 39 9
Fragments arranged by size:
684 412 241 236 178 114 95 83
83 62 59 39 24 9
BspHI T'CATG_A
Cuts at: 0 126 2319
Size: 126 2193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 725 1937 2319
Size: 725 1212 382
Fragments arranged by size:
1212 725 382
BsrDI GCAATG_nn'
Cuts at: 0 219 2319
Size: 219 2100
BsrFI r'CCGG_y
Cuts at: 0 1001 1184 1771 1981 2319
Size: 1001 183 587 210 338
Fragments arranged by size:
1001 587 338 210 183
BsrGI T'GTAC_A
Cuts at: 0 514 2319
Size: 514 1805
BssHII G'CGCG_C
Cuts at: 0 1382 1564 2319
Size: 1382 182 755
Fragments arranged by size:
1382 755 182
BssSI C'ACGA_G
Cuts at: 0 1573 2319
Size: 1573 746
BstAPI GCAn_nnn'nTGC
Cuts at: 0 224 469 1226 2319
Size: 224 245 757 1093
Fragments arranged by size:
1093 757 245 224
BstXI CCAn_nnnn'nTGG
Cuts at: 0 294 1362 2176 2319
Size: 294 1068 814 143
Fragments arranged by size:
1068 814 294 143
BstYI r'GATC_y
Cuts at: 0 2010 2126 2319
Size: 2010 116 193
Fragments arranged by size:
2010 193 116
BtgI C'CryG_G
Cuts at: 0 96 293 625 681 935 1394 1812 2319
Size: 96 197 332 56 254 459 418 507
Fragments arranged by size:
507 459 418 332 254 197 96 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1957 2319
Size: 1957 362
BtsI GCAGTG_nn'
Cuts at: 0 154 1978 2319
Size: 154 1824 341
Fragments arranged by size:
1824 341 154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2260 2295 2319
Size: 2260 35 24
Fragments arranged by size:
2260 35 24
DrdI GACnn_nn'nnGTC
Cuts at: 0 1308 2319
Size: 1308 1011
EaeI y'GGCC_r
Cuts at: 0 866 1400 1716 1724 1769 1809 1937 2319
Size: 866 534 316 8 45 40 128 382
Fragments arranged by size:
866 534 382 316 128 45 40 8
EagI C'GGCC_G
Cuts at: 0 1937 2319
Size: 1937 382
EarI CTCTTCn'nnn_
Cuts at: 0 848 1454 1472 1481 1490 2319
Size: 848 606 18 9 9 829
Fragments arranged by size:
848 829 606 18 9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 865 1083 2319
Size: 865 218 1236
Fragments arranged by size:
1236 865 218
EcoICRI GAG'CTC
Cuts at: 0 112 436 2269 2319
Size: 112 324 1833 50
Fragments arranged by size:
1833 324 112 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 420 626 1279 1576 1656 1980 2019
Size: 98 322 206 653 297 80 324 39
Cuts at: 2019 2319
Size: 300
Fragments arranged by size:
653 324 322 300 297 206 98 80
39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 891 1726 2319
Size: 891 835 593
Fragments arranged by size:
891 835 593
EcoO109I rG'GnC_Cy
Cuts at: 0 405 1692 1845 2319
Size: 405 1287 153 474
Fragments arranged by size:
1287 474 405 153
EcoRI G'AATT_C
Cuts at: 0 46 2319
Size: 46 2273
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 91 1074 2319
Size: 91 983 1245
Fragments arranged by size:
1245 983 91
FseI GG_CCGG'CC
Cuts at: 0 1005 2319
Size: 1005 1314
HaeII r_GCGC'y
Cuts at: 0 774 843 1266 1376 2319
Size: 774 69 423 110 943
Fragments arranged by size:
943 774 423 110 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 322 354 425 457 572 604 1309 1341
Size: 322 32 71 32 115 32 705 32
Cuts at: 1341 1747 1779 2284 2316 2319
Size: 406 32 505 32 3
Fragments arranged by size:
705 505 406 322 115 71 32 32
32 32 32 32 3
MluI A'CGCG_T
Cuts at: 0 1235 2319
Size: 1235 1084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1337 1760 2042 2319
Size: 1337 423 282 277
Fragments arranged by size:
1337 423 282 277
MscI TGG'CCA
Cuts at: 0 868 1402 2319
Size: 868 534 917
Fragments arranged by size:
917 868 534
MslI CAynn'nnrTG
Cuts at: 0 881 2174 2319
Size: 881 1293 145
Fragments arranged by size:
1293 881 145
MspA1I CmG'CkG
Cuts at: 0 627 990 1814 2141 2319
Size: 627 363 824 327 178
Fragments arranged by size:
824 627 363 327 178
NaeI GCC'GGC
Cuts at: 0 1003 1773 1983 2319
Size: 1003 770 210 336
Fragments arranged by size:
1003 770 336 210
NcoI C'CATG_G
Cuts at: 0 681 935 2319
Size: 681 254 1384
Fragments arranged by size:
1384 681 254
NgoMIV G'CCGG_C
Cuts at: 0 1001 1771 1981 2319
Size: 1001 770 210 338
Fragments arranged by size:
1001 770 338 210
NspI r_CATG'y
Cuts at: 0 515 1284 2319
Size: 515 769 1035
Fragments arranged by size:
1035 769 515
PciI A'CATG_T
Cuts at: 0 511 2319
Size: 511 1808
PflMI CCAn_nnn'nTGG
Cuts at: 0 545 1695 2319
Size: 545 1150 624
Fragments arranged by size:
1150 624 545
PfoI T'CCnGG_A
Cuts at: 0 1436 2319
Size: 1436 883
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1906 1938 1951 1983 2319
Size: 1906 32 13 32 336
Fragments arranged by size:
1906 336 32 32 13
PpuMI rG'GwC_Cy
Cuts at: 0 1692 2319
Size: 1692 627
PshAI GACnn'nnGTC
Cuts at: 0 1319 2319
Size: 1319 1000
PspOMI G'GGCC_C
Cuts at: 0 2184 2319
Size: 2184 135
RsrII CG'GwC_CG
Cuts at: 0 1782 2319
Size: 1782 537
SacI G_AGCT'C
Cuts at: 0 114 438 2271 2319
Size: 114 324 1833 48
Fragments arranged by size:
1833 324 114 48
SacII CC_GC'GG
Cuts at: 0 628 1815 2319
Size: 628 1187 504
Fragments arranged by size:
1187 628 504
SexAI A'CCwGG_T
Cuts at: 0 1172 1688 2319
Size: 1172 516 631
Fragments arranged by size:
1172 631 516
SgrAI Cr'CCGG_yG
Cuts at: 0 1184 2319
Size: 1184 1135
SmaI CCC'GGG
Cuts at: 0 1826 2109 2189 2319
Size: 1826 283 80 130
Fragments arranged by size:
1826 283 130 80
SmlI C'TyrA_G
Cuts at: 0 33 1387 1912 2270 2301 2311 2319
Size: 33 1354 525 358 31 10 8
Fragments arranged by size:
1354 525 358 33 31 10 8
SrfI GCCC'GGGC
Cuts at: 0 2189 2319
Size: 2189 130
StuI AGG'CCT
Cuts at: 0 1681 2319
Size: 1681 638
StyI C'CwwG_G
Cuts at: 0 681 935 2319
Size: 681 254 1384
Fragments arranged by size:
1384 681 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 795 886 1258 2319
Size: 795 91 372 1061
Fragments arranged by size:
1061 795 372 91
TatI w'GTAC_w
Cuts at: 0 514 1964 2319
Size: 514 1450 355
Fragments arranged by size:
1450 514 355
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1276 1308 2319
Size: 1276 32 1011
Fragments arranged by size:
1276 1011 32
XbaI T'CTAG_A
Cuts at: 0 349 2319
Size: 349 1970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1362 2319
Size: 1362 957
XhoI C'TCGA_G
Cuts at: 0 1912 2301 2319
Size: 1912 389 18
Fragments arranged by size:
1912 389 18
XmaI C'CCGG_G
Cuts at: 0 1824 2107 2187 2319
Size: 1824 283 80 132
Fragments arranged by size:
1824 283 132 80
XmnI GAAnn'nnTTC
Cuts at: 0 71 1921 2319
Size: 71 1850 398
Fragments arranged by size:
1850 398 71
ZraI GAC'GTC
Cuts at: 0 1635 2319
Size: 1635 684
Enzymes that do cut:
AatII AcuI AflII AflIII AleI AlwNI ApaI ApaLI
ApoI AscI AvaI BanI BanII BbsI BcgI BciVI
BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI HaeII Hin4I MluI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PciI
PflMI PfoI PpiI PpuMI PshAI PspOMI RsrII SacI
SacII SexAI SgrAI SmaI SmlI SrfI StuI StyI
TaqII TatI TstI XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AgeI AhdI AlfI
AloI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BclI BfrBI BglII BmrI BmtI BsaAI BsaWI BsiWI
BsmI BsmBI BspEI BsrBI BstBI BstEII BstZ17I Bsu36I
ClaI DraI DraIII EcoRV FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI NarI NdeI NheI NotI
NruI NsiI PacI PasI PmeI PmlI PsiI PspXI
PsrI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SnaBI SpeI SphI
SspI SwaI Tth111I