Restriction map for
pF1KSDA1810
(Linear) (Six-base) MAPSORT of: KIAA1810.seq Check: 9615 from: 1 to: 1695
KIAA1810.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 838 1695
Size: 838 857
AclI AA'CG_TT
Cuts at: 0 237 1695
Size: 237 1458
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 1442 1695
Size: 279 1163 253
Fragments arranged by size:
1163 279 253
AleI CACnn'nnGTG
Cuts at: 0 13 97 1695
Size: 13 84 1598
Fragments arranged by size:
1598 84 13
AlwNI CAG_nnn'CTG
Cuts at: 0 1120 1156 1168 1186 1341 1362 1570 1594
Size: 1120 36 12 18 155 21 208 24
Cuts at: 1594 1674 1695
Size: 80 21
Fragments arranged by size:
1120 208 155 80 36 24 21 21
18 12
ApaI G_GGCC'C
Cuts at: 0 1015 1442 1695
Size: 1015 427 253
Fragments arranged by size:
1015 427 253
ApaLI G'TGCA_C
Cuts at: 0 556 1695
Size: 556 1139
BanI G'GyrC_C
Cuts at: 0 748 1123 1658 1695
Size: 748 375 535 37
Fragments arranged by size:
748 535 375 37
BanII G_rGCy'C
Cuts at: 0 698 1015 1317 1442 1637 1652 1695
Size: 698 317 302 125 195 15 43
Fragments arranged by size:
698 317 302 195 125 43 15
BbeI G_GCGC'C
Cuts at: 0 1662 1695
Size: 1662 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 153 1695
Size: 153 1542
BbvCI CC'TCA_GC
Cuts at: 0 359 938 1695
Size: 359 579 757
Fragments arranged by size:
757 579 359
BciVI GTATCCnnnnn_n'
Cuts at: 0 683 1695
Size: 683 1012
BclI T'GATC_A
Cuts at: 0 548 1695
Size: 548 1147
BglI GCCn_nnn'nGGC
Cuts at: 0 34 1183 1446 1695
Size: 34 1149 263 249
Fragments arranged by size:
1149 263 249 34
Bme1580I G_kGCm'C
Cuts at: 0 560 1015 1274 1442 1609 1695
Size: 560 455 259 168 167 86
Fragments arranged by size:
560 455 259 168 167 86
BmrI ACTGGGnnnn_n'
Cuts at: 0 1088 1695
Size: 1088 607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 682 965 1339 1363 1571 1695
Size: 427 255 283 374 24 208 124
Fragments arranged by size:
427 374 283 255 208 124 24
Bpu10I CC'TnA_GC
Cuts at: 0 359 938 1044 1695
Size: 359 579 106 651
Fragments arranged by size:
651 579 359 106
BsaHI Gr'CG_yC
Cuts at: 0 1388 1659 1695
Size: 1388 271 36
Fragments arranged by size:
1388 271 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1040 1070 1695
Size: 1040 30 625
Fragments arranged by size:
1040 625 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 706 1052 1205 1318 1489 1497 1627 1695
Size: 706 346 153 113 171 8 130 68
Fragments arranged by size:
706 346 171 153 130 113 68 8
BseYI C'CCAG_C
Cuts at: 0 121 1039 1288 1695
Size: 121 918 249 407
Fragments arranged by size:
918 407 249 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1097 1695
Size: 412 685 598
Fragments arranged by size:
685 598 412
BsiHKAI G_wGCw'C
Cuts at: 0 227 404 560 698 1317 1652 1695
Size: 227 177 156 138 619 335 43
Fragments arranged by size:
619 335 227 177 156 138 43
Bsp1286I G_dGCh'C
Cuts at: 0 227 404 560 698 1015 1274 1317 1442
Size: 227 177 156 138 317 259 43 125
Cuts at: 1442 1609 1637 1652 1695
Size: 167 28 15 43
Fragments arranged by size:
317 259 227 177 167 156 138 125
43 43 28 15
BspHI T'CATG_A
Cuts at: 0 536 545 1695
Size: 536 9 1150
Fragments arranged by size:
1150 536 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 829 838 1695
Size: 829 9 857
Fragments arranged by size:
857 829 9
BsrBI CCG'CTC
Cuts at: 0 1332 1695
Size: 1332 363
BsrDI GCAATG_nn'
Cuts at: 0 710 1695
Size: 710 985
BsrFI r'CCGG_y
Cuts at: 0 1655 1695
Size: 1655 40
BsrGI T'GTAC_A
Cuts at: 0 137 476 524 1695
Size: 137 339 48 1171
Fragments arranged by size:
1171 339 137 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 725 1362 1695
Size: 725 637 333
Fragments arranged by size:
725 637 333
BstXI CCAn_nnnn'nTGG
Cuts at: 0 594 1695
Size: 594 1101
Bsu36I CC'TnA_GG
Cuts at: 0 1622 1695
Size: 1622 73
BtgI C'CryG_G
Cuts at: 0 434 1007 1695
Size: 434 573 688
Fragments arranged by size:
688 573 434
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 839 1695
Size: 839 856
BtsI GCAGTG_nn'
Cuts at: 0 20 1378 1695
Size: 20 1358 317
Fragments arranged by size:
1358 317 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 914 949 1473 1508 1695
Size: 914 35 524 35 187
Fragments arranged by size:
914 524 187 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 435 1695
Size: 435 1260
EaeI y'GGCC_r
Cuts at: 0 26 596 1695
Size: 26 570 1099
Fragments arranged by size:
1099 570 26
EarI CTCTTCn'nnn_
Cuts at: 0 322 329 337 728 1695
Size: 322 7 8 391 967
Fragments arranged by size:
967 391 322 8 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1642 1695
Size: 1642 53
EcoICRI GAG'CTC
Cuts at: 0 696 1315 1650 1695
Size: 696 619 335 45
Fragments arranged by size:
696 619 335 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 427 682 965 1339 1363 1442 1571
Size: 279 148 255 283 374 24 79 129
Cuts at: 1571 1695
Size: 124
Fragments arranged by size:
374 283 279 255 148 129 124 79
24
EcoO109I rG'GnC_Cy
Cuts at: 0 960 1012 1438 1439 1695
Size: 960 52 426 1 256
Fragments arranged by size:
960 426 256 52 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 210 1695
Size: 210 1485
HaeII r_GCGC'y
Cuts at: 0 40 1662 1695
Size: 40 1622 33
Fragments arranged by size:
1622 40 33
HincII GTy'rAC
Cuts at: 0 1464 1695
Size: 1464 231
KasI G'GCGC_C
Cuts at: 0 1658 1695
Size: 1658 37
MscI TGG'CCA
Cuts at: 0 598 1695
Size: 598 1097
MslI CAynn'nnrTG
Cuts at: 0 13 97 388 1695
Size: 13 84 291 1307
Fragments arranged by size:
1307 291 84 13
MspA1I CmG'CkG
Cuts at: 0 788 810 1117 1695
Size: 788 22 307 578
Fragments arranged by size:
788 578 307 22
NaeI GCC'GGC
Cuts at: 0 1657 1695
Size: 1657 38
NarI GG'CG_CC
Cuts at: 0 1659 1695
Size: 1659 36
NcoI C'CATG_G
Cuts at: 0 1007 1695
Size: 1007 688
NgoMIV G'CCGG_C
Cuts at: 0 1655 1695
Size: 1655 40
NspI r_CATG'y
Cuts at: 0 483 990 1695
Size: 483 507 705
Fragments arranged by size:
705 507 483
PasI CC'CwG_GG
Cuts at: 0 458 957 1695
Size: 458 499 738
Fragments arranged by size:
738 499 458
PflMI CCAn_nnn'nTGG
Cuts at: 0 354 705 1168 1186 1695
Size: 354 351 463 18 509
Fragments arranged by size:
509 463 354 351 18
PspOMI G'GGCC_C
Cuts at: 0 1011 1438 1695
Size: 1011 427 257
Fragments arranged by size:
1011 427 257
PstI C_TGCA'G
Cuts at: 0 1358 1403 1670 1695
Size: 1358 45 267 25
Fragments arranged by size:
1358 267 45 25
PvuII CAG'CTG
Cuts at: 0 788 1117 1695
Size: 788 329 578
Fragments arranged by size:
788 578 329
SacI G_AGCT'C
Cuts at: 0 698 1317 1652 1695
Size: 698 619 335 43
Fragments arranged by size:
698 619 335 43
SfcI C'TryA_G
Cuts at: 0 1354 1399 1487 1666 1695
Size: 1354 45 88 179 29
Fragments arranged by size:
1354 179 88 45 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1446 1695
Size: 1446 249
SfoI GGC'GCC
Cuts at: 0 1660 1695
Size: 1660 35
SgrAI Cr'CCGG_yG
Cuts at: 0 1655 1695
Size: 1655 40
StuI AGG'CCT
Cuts at: 0 148 1584 1695
Size: 148 1436 111
Fragments arranged by size:
1436 148 111
StyI C'CwwG_G
Cuts at: 0 1007 1433 1695
Size: 1007 426 262
Fragments arranged by size:
1007 426 262
TatI w'GTAC_w
Cuts at: 0 137 476 524 1695
Size: 137 339 48 1171
Fragments arranged by size:
1171 339 137 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 657 689 1040 1072 1695
Size: 657 32 351 32 623
Fragments arranged by size:
657 623 351 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 111 712 1695
Size: 111 601 983
Fragments arranged by size:
983 601 111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 123 834 1695
Size: 123 711 861
Fragments arranged by size:
861 711 123
Enzymes that do cut:
AarI AclI AcuI AleI AlwNI ApaI ApaLI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI Bme1580I
BmrI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstXI Bsu36I BtgI BtgZI BtsI CspCI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII
HincII KasI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PspOMI PstI PvuII SacI
SfcI SfiI SfoI SgrAI StuI StyI TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BcgI BfrBI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BssHII BssSI BstBI BstEII BstYI
BstZ17I ClaI DraI DrdI EagI EcoNI EcoRI EcoRV
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SgfI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII XbaI XhoI XmaI
XmnI ZraI