Restriction map for
pF1KSDA1813
(Linear) (Six-base) MAPSORT of: KIAA1813.seq Check: 6992 from: 1 to: 2007
KIAA1813.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 746 1167 1638 1815 2007
Size: 746 421 471 177 192
Fragments arranged by size:
746 471 421 192 177
AccI GT'mk_AC
Cuts at: 0 1482 2007
Size: 1482 525
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 572 1003 1378 2007
Size: 65 507 431 375 629
Fragments arranged by size:
629 507 431 375 65
AfeI AGC'GCT
Cuts at: 0 928 2007
Size: 928 1079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1607 2007
Size: 1607 400
AlwNI CAG_nnn'CTG
Cuts at: 0 18 595 632 917 1026 1167 1233 1368
Size: 18 577 37 285 109 141 66 135
Cuts at: 1368 1536 1902 2007
Size: 168 366 105
Fragments arranged by size:
577 366 285 168 141 135 109 105
66 37 18
ApaI G_GGCC'C
Cuts at: 0 158 556 803 1158 1719 2007
Size: 158 398 247 355 561 288
Fragments arranged by size:
561 398 355 288 247 158
AvaI C'yCGr_G
Cuts at: 0 115 329 464 500 782 893 1294 1496
Size: 115 214 135 36 282 111 401 202
Cuts at: 1496 1502 1931 2007
Size: 6 429 76
Fragments arranged by size:
429 401 282 214 202 135 115 111
76 36 6
AvrII C'CTAG_G
Cuts at: 0 235 852 2007
Size: 235 617 1155
Fragments arranged by size:
1155 617 235
BanI G'GyrC_C
Cuts at: 0 703 734 2007
Size: 703 31 1273
Fragments arranged by size:
1273 703 31
BanII G_rGCy'C
Cuts at: 0 158 474 556 803 1158 1295 1503 1619
Size: 158 316 82 247 355 137 208 116
Cuts at: 1619 1719 1940 2007
Size: 100 221 67
Fragments arranged by size:
355 316 247 221 208 158 137 116
100 82 67
BbvCI CC'TCA_GC
Cuts at: 0 1595 2007
Size: 1595 412
BglI GCCn_nnn'nGGC
Cuts at: 0 143 1810 1963 2007
Size: 143 1667 153 44
Fragments arranged by size:
1667 153 143 44
BglII A'GATC_T
Cuts at: 0 1346 2007
Size: 1346 661
BlpI GC'TnA_GC
Cuts at: 0 1559 1912 2007
Size: 1559 353 95
Fragments arranged by size:
1559 353 95
Bme1580I G_kGCm'C
Cuts at: 0 158 223 233 345 556 737 768 803
Size: 158 65 10 112 211 181 31 35
Cuts at: 803 1149 1158 1719 2007
Size: 346 9 561 288
Fragments arranged by size:
561 346 288 211 181 158 112 65
35 31 10 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 806 2007
Size: 806 1201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 53 421 816 1273 1531 1939 1945
Size: 52 1 368 395 457 258 408 6
Cuts at: 1945 2002 2007
Size: 57 5
Fragments arranged by size:
457 408 395 368 258 57 52 6
5 1
Bpu10I CC'TnA_GC
Cuts at: 0 1595 2007
Size: 1595 412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1327 2007
Size: 1327 680
BsaI GGTCTCn'nnnn_
Cuts at: 0 995 1306 2007
Size: 995 311 701
Fragments arranged by size:
995 701 311
BsaBI GATnn'nnATC
Cuts at: 0 424 2007
Size: 424 1583
BsaWI w'CCGG_w
Cuts at: 0 284 697 2007
Size: 284 413 1310
Fragments arranged by size:
1310 413 284
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 202 232 898 928 1303 1333 2007
Size: 202 30 666 30 375 30 674
Fragments arranged by size:
674 666 375 202 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 119 552 555 558 561 570 573 819
Size: 119 433 3 3 3 9 3 246
Cuts at: 819 1090 1324 1783 1999 2007
Size: 271 234 459 216 8
Fragments arranged by size:
459 433 271 246 234 216 119 9
8 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 222 301 620 868 1579 2007
Size: 222 79 319 248 711 428
Fragments arranged by size:
711 428 319 248 222 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 958 1414 1699 2007
Size: 31 927 456 285 308
Fragments arranged by size:
927 456 308 285 31
BsiEI CG_ry'CG
Cuts at: 0 1604 2007
Size: 1604 403
BsiHKAI G_wGCw'C
Cuts at: 0 844 1295 1503 1940 2007
Size: 844 451 208 437 67
Fragments arranged by size:
844 451 437 208 67
Bsp1286I G_dGCh'C
Cuts at: 0 158 223 233 345 474 556 737 768
Size: 158 65 10 112 129 82 181 31
Cuts at: 768 803 844 1149 1158 1295 1503 1619 1719
Size: 35 41 305 9 137 208 116 100
Cuts at: 1719 1940 2007
Size: 221 67
Fragments arranged by size:
305 221 208 181 158 137 129 116
112 100 82 67 65 41 35 31
10 9
BspEI T'CCGG_A
Cuts at: 0 284 2007
Size: 284 1723
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 746 1167 1638 1815 2007
Size: 746 421 471 177 192
Fragments arranged by size:
746 471 421 192 177
BsrBI CCG'CTC
Cuts at: 0 1080 1257 1278 1755 1797 2007
Size: 1080 177 21 477 42 210
Fragments arranged by size:
1080 477 210 177 42 21
BsrFI r'CCGG_y
Cuts at: 0 104 821 1874 1883 2007
Size: 104 717 1053 9 124
Fragments arranged by size:
1053 717 124 104 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 18 349 1902 2007
Size: 18 331 1553 105
Fragments arranged by size:
1553 331 105 18
BstXI CCAn_nnnn'nTGG
Cuts at: 0 178 754 2007
Size: 178 576 1253
Fragments arranged by size:
1253 576 178
BstYI r'GATC_y
Cuts at: 0 419 1346 2007
Size: 419 927 661
Fragments arranged by size:
927 661 419
BtgI C'CryG_G
Cuts at: 0 141 360 747 2007
Size: 141 219 387 1260
Fragments arranged by size:
1260 387 219 141
BtsI GCAGTG_nn'
Cuts at: 0 53 318 766 891 1991 2007
Size: 53 265 448 125 1100 16
Fragments arranged by size:
1100 448 265 125 53 16
EaeI y'GGCC_r
Cuts at: 0 2 660 819 1601 1955 2007
Size: 2 658 159 782 354 52
Fragments arranged by size:
782 658 354 159 52 2
EagI C'GGCC_G
Cuts at: 0 1601 2007
Size: 1601 406
EarI CTCTTCn'nnn_
Cuts at: 0 579 591 2007
Size: 579 12 1416
Fragments arranged by size:
1416 579 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 157 418 1672 2007
Size: 157 261 1254 335
Fragments arranged by size:
1254 335 261 157
EcoICRI GAG'CTC
Cuts at: 0 1293 1501 1938 2007
Size: 1293 208 437 69
Fragments arranged by size:
1293 437 208 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 53 65 421 572 816 1003 1273
Size: 52 1 12 356 151 244 187 270
Cuts at: 1273 1378 1531 1939 1945 2002 2007
Size: 105 153 408 6 57 5
Fragments arranged by size:
408 356 270 244 187 153 151 105
57 52 12 6 5 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1117 2007
Size: 1117 890
EcoO109I rG'GnC_Cy
Cuts at: 0 496 552 553 789 800 966 1110 1965
Size: 496 56 1 236 11 166 144 855
Cuts at: 1965 2007
Size: 42
Fragments arranged by size:
855 496 236 166 144 56 42 11
1
FseI GG_CCGG'CC
Cuts at: 0 108 2007
Size: 108 1899
HaeII r_GCGC'y
Cuts at: 0 930 1263 1432 2007
Size: 930 333 169 575
Fragments arranged by size:
930 575 333 169
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 898 930 2007
Size: 898 32 1077
Fragments arranged by size:
1077 898 32
MfeI C'AATT_G
Cuts at: 0 1207 2007
Size: 1207 800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 677 1568 2007
Size: 677 891 439
Fragments arranged by size:
891 677 439
MscI TGG'CCA
Cuts at: 0 4 2007
Size: 4 2003
MslI CAynn'nnrTG
Cuts at: 0 1867 2007
Size: 1867 140
MspA1I CmG'CkG
Cuts at: 0 216 226 339 362 543 592 1026 1086
Size: 216 10 113 23 181 49 434 60
Cuts at: 1086 1143 1152 1164 1173 1188 1230 1251 1287
Size: 57 9 12 9 15 42 21 36
Cuts at: 1287 1368 1566 1639 1683 1749 1776 1848 2007
Size: 81 198 73 44 66 27 72 159
Fragments arranged by size:
434 216 198 181 159 113 81 73
72 66 60 57 49 44 42 36
27 23 21 15 12 10 9 9
NaeI GCC'GGC
Cuts at: 0 106 2007
Size: 106 1901
NcoI C'CATG_G
Cuts at: 0 747 2007
Size: 747 1260
NgoMIV G'CCGG_C
Cuts at: 0 104 2007
Size: 104 1903
PfoI T'CCnGG_A
Cuts at: 0 983 1610 2007
Size: 983 627 397
Fragments arranged by size:
983 627 397
PpuMI rG'GwC_Cy
Cuts at: 0 496 789 2007
Size: 496 293 1218
Fragments arranged by size:
1218 496 293
PspOMI G'GGCC_C
Cuts at: 0 154 552 799 1154 1715 2007
Size: 154 398 247 355 561 292
Fragments arranged by size:
561 398 355 292 247 154
PspXI vC'TCGA_Gb
Cuts at: 0 464 2007
Size: 464 1543
PstI C_TGCA'G
Cuts at: 0 1172 1178 1193 1238 1541 1748 1853 1907
Size: 1172 6 15 45 303 207 105 54
Cuts at: 1907 2007
Size: 100
Fragments arranged by size:
1172 303 207 105 100 54 45 15
6
PvuII CAG'CTG
Cuts at: 0 226 543 592 1026 1164 1173 1188 1230
Size: 226 317 49 434 138 9 15 42
Cuts at: 1230 1251 1368 1566 1848 2007
Size: 21 117 198 282 159
Fragments arranged by size:
434 317 282 226 198 159 138 117
49 42 21 15 9
SacI G_AGCT'C
Cuts at: 0 1295 1503 1940 2007
Size: 1295 208 437 67
Fragments arranged by size:
1295 437 208 67
SacII CC_GC'GG
Cuts at: 0 363 2007
Size: 363 1644
SanDI GG'GwC_CC
Cuts at: 0 789 2007
Size: 789 1218
SfcI C'TryA_G
Cuts at: 0 690 1168 1174 1189 1234 1483 1537 1744
Size: 690 478 6 15 45 249 54 207
Cuts at: 1744 1849 1903 2007
Size: 105 54 104
Fragments arranged by size:
690 478 249 207 105 104 54 54
45 15 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1963 2007
Size: 1963 44
SmaI CCC'GGG
Cuts at: 0 117 1933 2007
Size: 117 1816 74
Fragments arranged by size:
1816 117 74
SmlI C'TyrA_G
Cuts at: 0 464 1306 2007
Size: 464 842 701
Fragments arranged by size:
842 701 464
StyI C'CwwG_G
Cuts at: 0 235 747 852 1271 2007
Size: 235 512 105 419 736
Fragments arranged by size:
736 512 419 235 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 202 234 727 759 1303 1335 2007
Size: 202 32 493 32 544 32 672
Fragments arranged by size:
672 544 493 202 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 291 2007
Size: 291 1716
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 148 814 2007
Size: 148 666 1193
Fragments arranged by size:
1193 666 148
XhoI C'TCGA_G
Cuts at: 0 464 2007
Size: 464 1543
XmaI C'CCGG_G
Cuts at: 0 115 1931 2007
Size: 115 1816 76
Fragments arranged by size:
1816 115 76
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AlwNI ApaI AvaI
AvrII BanI BanII BbvCI BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrBI BsrFI BstAPI BstXI BstYI BtgI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI
HaeII Hin4I MfeI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV PfoI PpuMI PspOMI PspXI PstI PvuII
SacI SacII SanDI SfcI SfiI SmaI SmlI StyI
TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI BaeI BamHI
BbeI BbsI BcgI BciVI BclI BfrBI BmgBI BmtI
BplI BsaAI BsaHI BsiWI BsmI BsmBI BspHI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI
FspI FspAI HincII HindIII HpaI KasI KpnI MluI
NarI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI XbaI XmnI ZraI