Restriction map for
pF1KSDA1817
(Linear) (Six-base) MAPSORT of: KIAA1817.seq Check: 3128 from: 1 to: 2598
KIAA1817.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 798 1854 1884 2484 2598
Size: 798 1056 30 600 114
Fragments arranged by size:
1056 798 600 114 30
AccI GT'mk_AC
Cuts at: 0 1502 2598
Size: 1502 1096
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 890 2598
Size: 307 583 1708
Fragments arranged by size:
1708 583 307
AflIII A'CryG_T
Cuts at: 0 1390 2598
Size: 1390 1208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1135 1906 2598
Size: 1135 771 692
Fragments arranged by size:
1135 771 692
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 995 1027 2598
Size: 995 32 1571
Fragments arranged by size:
1571 995 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1084 1276 1899 2262 2506 2598
Size: 1084 192 623 363 244 92
Fragments arranged by size:
1084 623 363 244 192 92
ApaI G_GGCC'C
Cuts at: 0 2013 2598
Size: 2013 585
ApoI r'AATT_y
Cuts at: 0 327 438 2598
Size: 327 111 2160
Fragments arranged by size:
2160 327 111
AvaI C'yCGr_G
Cuts at: 0 641 740 767 896 1343 1398 1681 2101
Size: 641 99 27 129 447 55 283 420
Cuts at: 2101 2598
Size: 497
Fragments arranged by size:
641 497 447 420 283 129 99 55
27
BanI G'GyrC_C
Cuts at: 0 559 1057 1869 2598
Size: 559 498 812 729
Fragments arranged by size:
812 729 559 498
BanII G_rGCy'C
Cuts at: 0 88 673 754 781 858 1422 1814 2013
Size: 88 585 81 27 77 564 392 199
Cuts at: 2013 2180 2221 2598
Size: 167 41 377
Fragments arranged by size:
585 564 392 377 199 167 88 81
77 41 27
BbvCI CC'TCA_GC
Cuts at: 0 52 1294 2387 2598
Size: 52 1242 1093 211
Fragments arranged by size:
1242 1093 211 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2092 2126 2598
Size: 2092 34 472
Fragments arranged by size:
2092 472 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1187 2008 2598
Size: 1187 821 590
Fragments arranged by size:
1187 821 590
BglII A'GATC_T
Cuts at: 0 2135 2598
Size: 2135 463
BlpI GC'TnA_GC
Cuts at: 0 2263 2598
Size: 2263 335
Bme1580I G_kGCm'C
Cuts at: 0 562 1220 2013 2115 2598
Size: 562 658 793 102 483
Fragments arranged by size:
793 658 562 483 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 299 2598
Size: 299 2299
BmtI G_CTAG'C
Cuts at: 0 2161 2598
Size: 2161 437
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 60 2240 2598
Size: 60 2180 358
Fragments arranged by size:
2180 358 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 296 552 613 1027 1061 1357 1615
Size: 247 49 256 61 414 34 296 258
Cuts at: 1615 2598
Size: 983
Fragments arranged by size:
983 414 296 258 256 247 61 49
34
Bpu10I CC'TnA_GC
Cuts at: 0 52 1294 2387 2598
Size: 52 1242 1093 211
Fragments arranged by size:
1242 1093 211 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1036 2149 2173 2598
Size: 1036 1113 24 425
Fragments arranged by size:
1113 1036 425 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 48 310 360 619 794 1595 1607 1636
Size: 48 262 50 259 175 801 12 29
Cuts at: 1636 2272 2598
Size: 636 326
Fragments arranged by size:
801 636 326 262 259 175 50 48
29 12
BsaAI yAC'GTr
Cuts at: 0 1391 2598
Size: 1391 1207
BsaWI w'CCGG_w
Cuts at: 0 1694 2598
Size: 1694 904
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 64 94 2598
Size: 64 30 2504
Fragments arranged by size:
2504 64 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1699 2404 2598
Size: 1699 705 194
Fragments arranged by size:
1699 705 194
BseYI C'CCAG_C
Cuts at: 0 205 342 673 700 727 754 1219 1608
Size: 205 137 331 27 27 27 465 389
Cuts at: 1608 2318 2598
Size: 710 280
Fragments arranged by size:
710 465 389 331 280 205 137 27
27 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 385 1141 2402 2598
Size: 368 17 756 1261 196
Fragments arranged by size:
1261 756 368 196 17
BsiEI CG_ry'CG
Cuts at: 0 140 1357 2598
Size: 140 1217 1241
Fragments arranged by size:
1241 1217 140
BsiHKAI G_wGCw'C
Cuts at: 0 312 375 601 817 1422 1814 2180 2566
Size: 312 63 226 216 605 392 366 386
Cuts at: 2566 2598
Size: 32
Fragments arranged by size:
605 392 386 366 312 226 216 63
32
BsmI GAATG_Cn'
Cuts at: 0 1319 2598
Size: 1319 1279
Bsp1286I G_dGCh'C
Cuts at: 0 88 312 375 562 601 673 754 781
Size: 88 224 63 187 39 72 81 27
Cuts at: 781 817 858 1220 1422 1814 2013 2115 2180
Size: 36 41 362 202 392 199 102 65
Cuts at: 2180 2221 2566 2598
Size: 41 345 32
Fragments arranged by size:
392 362 345 224 202 199 187 102
88 81 72 65 63 41 41 39
36 32 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 30 71 798 1076 1577 1854 1884 1921
Size: 30 41 727 278 501 277 30 37
Cuts at: 1921 2484 2598
Size: 563 114
Fragments arranged by size:
727 563 501 278 277 114 41 37
30 30
BsrBI CCG'CTC
Cuts at: 0 969 2598
Size: 969 1629
BsrFI r'CCGG_y
Cuts at: 0 803 1190 2598
Size: 803 387 1408
Fragments arranged by size:
1408 803 387
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1736 1739 1789 1869 2506 2598
Size: 1736 3 50 80 637 92
Fragments arranged by size:
1736 637 92 80 50 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 715 1572 1662 2020 2598
Size: 715 857 90 358 578
Fragments arranged by size:
857 715 578 358 90
BstYI r'GATC_y
Cuts at: 0 1835 2135 2598
Size: 1835 300 463
Fragments arranged by size:
1835 463 300
Bsu36I CC'TnA_GG
Cuts at: 0 1931 2598
Size: 1931 667
BtgI C'CryG_G
Cuts at: 0 656 2598
Size: 656 1942
BtsI GCAGTG_nn'
Cuts at: 0 120 176 276 1675 2092 2598
Size: 120 56 100 1399 417 506
Fragments arranged by size:
1399 506 417 120 100 56
EaeI y'GGCC_r
Cuts at: 0 137 717 1086 1192 1664 1988 2093 2201
Size: 137 580 369 106 472 324 105 108
Cuts at: 2201 2336 2598
Size: 135 262
Fragments arranged by size:
580 472 369 324 262 137 135 108
106 105
EagI C'GGCC_G
Cuts at: 0 137 2598
Size: 137 2461
EarI CTCTTCn'nnn_
Cuts at: 0 29 363 617 2342 2598
Size: 29 334 254 1725 256
Fragments arranged by size:
1725 334 256 254 29
EcoICRI GAG'CTC
Cuts at: 0 1420 1812 2178 2598
Size: 1420 392 366 420
Fragments arranged by size:
1420 420 392 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 296 307 552 613 890 1027 1061
Size: 247 49 11 245 61 277 137 34
Cuts at: 1061 1357 1615 2598
Size: 296 258 983
Fragments arranged by size:
983 296 277 258 247 245 137 61
49 34 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 67 2598
Size: 67 2531
EcoO109I rG'GnC_Cy
Cuts at: 0 874 901 1129 1481 1576 1651 1886 2598
Size: 874 27 228 352 95 75 235 712
Fragments arranged by size:
874 712 352 235 228 95 75 27
EcoRI G'AATT_C
Cuts at: 0 327 2598
Size: 327 2271
FseI GG_CCGG'CC
Cuts at: 0 1194 2598
Size: 1194 1404
HaeII r_GCGC'y
Cuts at: 0 2325 2598
Size: 2325 273
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1339 1371 1468 1500 1798 1830 2598
Size: 1339 32 97 32 298 32 768
Fragments arranged by size:
1339 768 298 97 32 32 32
HindIII A'AGCT_T
Cuts at: 0 91 832 2149 2598
Size: 91 741 1317 449
Fragments arranged by size:
1317 741 449 91
MscI TGG'CCA
Cuts at: 0 719 1990 2095 2203 2338 2598
Size: 719 1271 105 108 135 260
Fragments arranged by size:
1271 719 260 135 108 105
MslI CAynn'nnrTG
Cuts at: 0 2018 2598
Size: 2018 580
MspA1I CmG'CkG
Cuts at: 0 342 591 677 704 758 1091 1097 1160
Size: 342 249 86 27 54 333 6 63
Cuts at: 1160 2386 2506 2598
Size: 1226 120 92
Fragments arranged by size:
1226 342 333 249 120 92 86 63
54 27 6
NaeI GCC'GGC
Cuts at: 0 805 1192 2598
Size: 805 387 1406
Fragments arranged by size:
1406 805 387
NcoI C'CATG_G
Cuts at: 0 656 2598
Size: 656 1942
NgoMIV G'CCGG_C
Cuts at: 0 803 1190 2598
Size: 803 387 1408
Fragments arranged by size:
1408 803 387
NheI G'CTAG_C
Cuts at: 0 2157 2598
Size: 2157 441
NspI r_CATG'y
Cuts at: 0 1140 1527 2232 2598
Size: 1140 387 705 366
Fragments arranged by size:
1140 705 387 366
PasI CC'CwG_GG
Cuts at: 0 1518 1532 2598
Size: 1518 14 1066
Fragments arranged by size:
1518 1066 14
PflMI CCAn_nnn'nTGG
Cuts at: 0 532 1084 1276 2598
Size: 532 552 192 1322
Fragments arranged by size:
1322 552 532 192
PmlI CAC'GTG
Cuts at: 0 1391 2598
Size: 1391 1207
PpuMI rG'GwC_Cy
Cuts at: 0 901 1576 1886 2598
Size: 901 675 310 712
Fragments arranged by size:
901 712 675 310
PspOMI G'GGCC_C
Cuts at: 0 2009 2598
Size: 2009 589
PspXI vC'TCGA_Gb
Cuts at: 0 767 2598
Size: 767 1831
PstI C_TGCA'G
Cuts at: 0 551 668 986 1096 1895 1918 2511 2598
Size: 551 117 318 110 799 23 593 87
Fragments arranged by size:
799 593 551 318 117 110 87 23
PvuI CG_AT'CG
Cuts at: 0 1357 2598
Size: 1357 1241
PvuII CAG'CTG
Cuts at: 0 342 591 677 704 758 1097 1160 2386
Size: 342 249 86 27 54 339 63 1226
Cuts at: 2386 2506 2598
Size: 120 92
Fragments arranged by size:
1226 342 339 249 120 92 86 63
54 27
SacI G_AGCT'C
Cuts at: 0 1422 1814 2180 2598
Size: 1422 392 366 418
Fragments arranged by size:
1422 418 392 366
SanDI GG'GwC_CC
Cuts at: 0 1576 2598
Size: 1576 1022
SapI GCTCTTCn'nnn_
Cuts at: 0 29 2598
Size: 29 2569
SbfI CC_TGCA'GG
Cuts at: 0 1895 2598
Size: 1895 703
SfcI C'TryA_G
Cuts at: 0 145 497 547 664 982 1092 1449 1891
Size: 145 352 50 117 318 110 357 442
Cuts at: 1891 1914 2507 2598
Size: 23 593 91
Fragments arranged by size:
593 442 357 352 318 145 117 110
91 50 23
SmlI C'TyrA_G
Cuts at: 0 740 767 1015 2152 2164 2598
Size: 740 27 248 1137 12 434
Fragments arranged by size:
1137 740 434 248 27 12
SphI G_CATG'C
Cuts at: 0 1527 2598
Size: 1527 1071
StuI AGG'CCT
Cuts at: 0 1936 2598
Size: 1936 662
StyI C'CwwG_G
Cuts at: 0 68 573 656 1541 1571 2339 2598
Size: 68 505 83 885 30 768 259
Fragments arranged by size:
885 768 505 259 83 68 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 334 390 520 618 1219 2587 2598
Size: 334 56 130 98 601 1368 11
Fragments arranged by size:
1368 601 334 130 98 56 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2511 2543 2598
Size: 2511 32 55
Fragments arranged by size:
2511 55 32
Tth111I GACn'n_nGTC
Cuts at: 0 1246 2598
Size: 1246 1352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 320 2598
Size: 320 2278
XhoI C'TCGA_G
Cuts at: 0 740 767 2598
Size: 740 27 1831
Fragments arranged by size:
1831 740 27
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AloI AlwNI ApaI
ApoI AvaI BanI BanII BbvCI BcgI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI
BstYI Bsu36I BtgI BtsI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FseI HaeII Hin4I HindIII
MscI MslI MspA1I NaeI NcoI NgoMIV NheI NspI
PasI PflMI PmlI PpuMI PspOMI PspXI PstI PvuI
PvuII SacI SanDI SapI SbfI SfcI SmlI SphI
StuI StyI TaqII TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbsI BciVI BclI BfrBI BmgBI BsaBI BsaHI BsiWI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EciI EcoRV FalI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MmeI NarI NdeI NotI NruI
NsiI PacI PciI PfoI PmeI PpiI PshAI PsiI
PsrI RsrII SacII SalI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TatI XbaI XmaI XmnI ZraI