Restriction map for
pF1KSDA1819
(Linear) (Six-base) MAPSORT of: KIAA1819.seq Check: 6601 from: 1 to: 3459
KIAA1819.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 419 2970 3459
Size: 419 2551 489
Fragments arranged by size:
2551 489 419
AatII G_ACGT'C
Cuts at: 0 2932 3459
Size: 2932 527
AccI GT'mk_AC
Cuts at: 0 2539 3459
Size: 2539 920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1199 2032 2050 2627 3459
Size: 1199 833 18 577 832
Fragments arranged by size:
1199 833 832 577 18
AflIII A'CryG_T
Cuts at: 0 1538 3459
Size: 1538 1921
AleI CACnn'nnGTG
Cuts at: 0 3299 3459
Size: 3299 160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2447 3459
Size: 2447 1012
AlwNI CAG_nnn'CTG
Cuts at: 0 228 411 924 2322 2988 3400 3459
Size: 228 183 513 1398 666 412 59
Fragments arranged by size:
1398 666 513 412 228 183 59
ApaI G_GGCC'C
Cuts at: 0 1126 3349 3459
Size: 1126 2223 110
Fragments arranged by size:
2223 1126 110
ApaLI G'TGCA_C
Cuts at: 0 94 3459
Size: 94 3365
ApoI r'AATT_y
Cuts at: 0 614 2805 3459
Size: 614 2191 654
Fragments arranged by size:
2191 654 614
AvaI C'yCGr_G
Cuts at: 0 87 3459
Size: 87 3372
AvrII C'CTAG_G
Cuts at: 0 1057 3459
Size: 1057 2402
BamHI G'GATC_C
Cuts at: 0 906 3459
Size: 906 2553
BanI G'GyrC_C
Cuts at: 0 1571 3459
Size: 1571 1888
BanII G_rGCy'C
Cuts at: 0 65 79 126 196 1126 1483 1652 1894
Size: 65 14 47 70 930 357 169 242
Cuts at: 1894 3349 3459
Size: 1455 110
Fragments arranged by size:
1455 930 357 242 169 110 70 65
47 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 3397 3459
Size: 3397 62
BbvCI CC'TCA_GC
Cuts at: 0 1139 1144 1557 3322 3459
Size: 1139 5 413 1765 137
Fragments arranged by size:
1765 1139 413 137 5
BciVI GTATCCnnnnn_n'
Cuts at: 0 1299 2095 3459
Size: 1299 796 1364
Fragments arranged by size:
1364 1299 796
BclI T'GATC_A
Cuts at: 0 986 1088 2858 3459
Size: 986 102 1770 601
Fragments arranged by size:
1770 986 601 102
BglI GCCn_nnn'nGGC
Cuts at: 0 55 1130 1278 3459
Size: 55 1075 148 2181
Fragments arranged by size:
2181 1075 148 55
BlpI GC'TnA_GC
Cuts at: 0 1235 3151 3459
Size: 1235 1916 308
Fragments arranged by size:
1916 1235 308
Bme1580I G_kGCm'C
Cuts at: 0 98 1126 1574 3349 3459
Size: 98 1028 448 1775 110
Fragments arranged by size:
1775 1028 448 110 98
BmrI ACTGGGnnnn_n'
Cuts at: 0 2159 2488 2895 3459
Size: 2159 329 407 564
Fragments arranged by size:
2159 564 407 329
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 651 1250 3459
Size: 651 599 2209
Fragments arranged by size:
2209 651 599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 911 997 2293 2369 3459
Size: 499 412 86 1296 76 1090
Fragments arranged by size:
1296 1090 499 412 86 76
Bpu10I CC'TnA_GC
Cuts at: 0 154 229 911 1139 1144 1557 2029 3322
Size: 154 75 682 228 5 413 472 1293
Cuts at: 3322 3459
Size: 137
Fragments arranged by size:
1293 682 472 413 228 154 137 75
5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2879 3459
Size: 2879 580
BsaI GGTCTCn'nnnn_
Cuts at: 0 2793 3063 3459
Size: 2793 270 396
Fragments arranged by size:
2793 396 270
BsaHI Gr'CG_yC
Cuts at: 0 2929 3238 3459
Size: 2929 309 221
Fragments arranged by size:
2929 309 221
BsaWI w'CCGG_w
Cuts at: 0 1252 3459
Size: 1252 2207
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 297 327 738 768 1188 1218 2095 2125
Size: 297 30 411 30 420 30 877 30
Cuts at: 2125 2203 2233 2291 2321 3459
Size: 78 30 58 30 1138
Fragments arranged by size:
1138 877 420 411 297 78 58 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1062 1127 1386 3026 3459
Size: 1062 65 259 1640 433
Fragments arranged by size:
1640 1062 433 259 65
BseYI C'CCAG_C
Cuts at: 0 1298 1441 1638 2222 3241 3459
Size: 1298 143 197 584 1019 218
Fragments arranged by size:
1298 1019 584 218 197 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2861 3459
Size: 2861 598
BsiHKAI G_wGCw'C
Cuts at: 0 98 196 1054 1483 1894 3459
Size: 98 98 858 429 411 1565
Fragments arranged by size:
1565 858 429 411 98 98
BsmI GAATG_Cn'
Cuts at: 0 1168 2564 3459
Size: 1168 1396 895
Fragments arranged by size:
1396 1168 895
BsmBI CGTCTCn'nnnn_
Cuts at: 0 875 1208 3459
Size: 875 333 2251
Fragments arranged by size:
2251 875 333
Bsp1286I G_dGCh'C
Cuts at: 0 65 79 98 126 196 1054 1126 1483
Size: 65 14 19 28 70 858 72 357
Cuts at: 1483 1574 1652 1894 3349 3459
Size: 91 78 242 1455 110
Fragments arranged by size:
1455 858 357 242 110 91 78 72
70 65 28 19 14
BspEI T'CCGG_A
Cuts at: 0 1252 3459
Size: 1252 2207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 419 2970 3459
Size: 419 2551 489
Fragments arranged by size:
2551 489 419
BsrDI GCAATG_nn'
Cuts at: 0 768 2087 3459
Size: 768 1319 1372
Fragments arranged by size:
1372 1319 768
BsrFI r'CCGG_y
Cuts at: 0 1549 3459
Size: 1549 1910
BsrGI T'GTAC_A
Cuts at: 0 237 1541 3459
Size: 237 1304 1918
Fragments arranged by size:
1918 1304 237
BstAPI GCAn_nnn'nTGC
Cuts at: 0 411 2319 3459
Size: 411 1908 1140
Fragments arranged by size:
1908 1140 411
BstEII G'GTnAC_C
Cuts at: 0 2033 3459
Size: 2033 1426
BstYI r'GATC_y
Cuts at: 0 906 1605 2773 3459
Size: 906 699 1168 686
Fragments arranged by size:
1168 906 699 686
Bsu36I CC'TnA_GG
Cuts at: 0 1128 1601 2730 3121 3459
Size: 1128 473 1129 391 338
Fragments arranged by size:
1129 1128 473 391 338
BtgI C'CryG_G
Cuts at: 0 1073 1175 1643 2716 3110 3459
Size: 1073 102 468 1073 394 349
Fragments arranged by size:
1073 1073 468 394 349 102
BtsI GCAGTG_nn'
Cuts at: 0 290 444 2986 3035 3065 3306 3459
Size: 290 154 2542 49 30 241 153
Fragments arranged by size:
2542 290 241 154 153 49 30
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 414 449 3459
Size: 414 35 3010
Fragments arranged by size:
3010 414 35
DraIII CAC_nnn'GTG
Cuts at: 0 2142 3459
Size: 2142 1317
EaeI y'GGCC_r
Cuts at: 0 246 840 1178 1512 2719 3459
Size: 246 594 338 334 1207 740
Fragments arranged by size:
1207 740 594 338 334 246
EcoICRI GAG'CTC
Cuts at: 0 194 1481 1892 3459
Size: 194 1287 411 1567
Fragments arranged by size:
1567 1287 411 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 911 997 1199 2032 2050 2293 2369
Size: 499 412 86 202 833 18 243 76
Cuts at: 2369 2627 3459
Size: 258 832
Fragments arranged by size:
833 832 499 412 258 243 202 86
76 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1244 2729 3459
Size: 1244 1485 730
Fragments arranged by size:
1485 1244 730
EcoO109I rG'GnC_Cy
Cuts at: 0 24 47 692 1061 1122 1123 1148 2161
Size: 24 23 645 369 61 1 25 1013
Cuts at: 2161 3115 3346 3459
Size: 954 231 113
Fragments arranged by size:
1013 954 645 369 231 113 61 25
24 23 1
EcoRI G'AATT_C
Cuts at: 0 614 3459
Size: 614 2845
EcoRV GAT'ATC
Cuts at: 0 606 3459
Size: 606 2853
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2039 2054 3459
Size: 2039 15 1405
Fragments arranged by size:
2039 1405 15
FseI GG_CCGG'CC
Cuts at: 0 1553 3459
Size: 1553 1906
HaeII r_GCGC'y
Cuts at: 0 16 117 3459
Size: 16 101 3342
Fragments arranged by size:
3342 101 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 717 749 802 834 2093 2115 2122 2125
Size: 717 32 53 32 1259 22 7 3
Cuts at: 2125 2147 2154 3459
Size: 22 7 1305
Fragments arranged by size:
1305 1259 717 53 32 32 22 22
7 7 3
HincII GTy'rAC
Cuts at: 0 546 3050 3459
Size: 546 2504 409
Fragments arranged by size:
2504 546 409
HindIII A'AGCT_T
Cuts at: 0 2463 3459
Size: 2463 996
HpaI GTT'AAC
Cuts at: 0 546 3050 3459
Size: 546 2504 409
Fragments arranged by size:
2504 546 409
MfeI C'AATT_G
Cuts at: 0 2245 2656 2683 2794 3459
Size: 2245 411 27 111 665
Fragments arranged by size:
2245 665 411 111 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2891 3459
Size: 2891 568
MscI TGG'CCA
Cuts at: 0 248 842 1180 3459
Size: 248 594 338 2279
Fragments arranged by size:
2279 594 338 248
MslI CAynn'nnrTG
Cuts at: 0 836 949 1577 3299 3459
Size: 836 113 628 1722 160
Fragments arranged by size:
1722 836 628 160 113
MspA1I CmG'CkG
Cuts at: 0 1143 1159 1511 3459
Size: 1143 16 352 1948
Fragments arranged by size:
1948 1143 352 16
NaeI GCC'GGC
Cuts at: 0 1551 3459
Size: 1551 1908
NcoI C'CATG_G
Cuts at: 0 1073 1175 1643 3110 3459
Size: 1073 102 468 1467 349
Fragments arranged by size:
1467 1073 468 349 102
NgoMIV G'CCGG_C
Cuts at: 0 1549 3459
Size: 1549 1910
NspI r_CATG'y
Cuts at: 0 1339 1542 2832 3459
Size: 1339 203 1290 627
Fragments arranged by size:
1339 1290 627 203
PasI CC'CwG_GG
Cuts at: 0 481 1563 3459
Size: 481 1082 1896
Fragments arranged by size:
1896 1082 481
PciI A'CATG_T
Cuts at: 0 1538 3459
Size: 1538 1921
PflMI CCAn_nnn'nTGG
Cuts at: 0 1159 2200 2484 2820 3459
Size: 1159 1041 284 336 639
Fragments arranged by size:
1159 1041 639 336 284
PpuMI rG'GwC_Cy
Cuts at: 0 692 3115 3459
Size: 692 2423 344
Fragments arranged by size:
2423 692 344
PshAI GACnn'nnGTC
Cuts at: 0 1031 2370 3459
Size: 1031 1339 1089
Fragments arranged by size:
1339 1089 1031
PsiI TTA'TAA
Cuts at: 0 2393 3459
Size: 2393 1066
PspOMI G'GGCC_C
Cuts at: 0 1122 3345 3459
Size: 1122 2223 114
Fragments arranged by size:
2223 1122 114
PstI C_TGCA'G
Cuts at: 0 642 920 2937 2967 3459
Size: 642 278 2017 30 492
Fragments arranged by size:
2017 642 492 278 30
PvuII CAG'CTG
Cuts at: 0 1143 1159 3459
Size: 1143 16 2300
Fragments arranged by size:
2300 1143 16
SacI G_AGCT'C
Cuts at: 0 196 1483 1894 3459
Size: 196 1287 411 1565
Fragments arranged by size:
1565 1287 411 196
SanDI GG'GwC_CC
Cuts at: 0 3115 3459
Size: 3115 344
SexAI A'CCwGG_T
Cuts at: 0 1413 3459
Size: 1413 2046
SfcI C'TryA_G
Cuts at: 0 305 638 916 2269 2573 2933 2963 3459
Size: 305 333 278 1353 304 360 30 496
Fragments arranged by size:
1353 496 360 333 305 304 278 30
SmlI C'TyrA_G
Cuts at: 0 2894 3459
Size: 2894 565
SphI G_CATG'C
Cuts at: 0 1339 2832 3459
Size: 1339 1493 627
Fragments arranged by size:
1493 1339 627
SspI AAT'ATT
Cuts at: 0 861 3459
Size: 861 2598
StyI C'CwwG_G
Cuts at: 0 66 278 1057 1073 1175 1643 3110 3459
Size: 66 212 779 16 102 468 1467 349
Fragments arranged by size:
1467 779 468 349 212 102 66 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1652 1774 3273 3459
Size: 1652 122 1499 186
Fragments arranged by size:
1652 1499 186 122
TatI w'GTAC_w
Cuts at: 0 237 1541 3459
Size: 237 1304 1918
Fragments arranged by size:
1918 1304 237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1188 1220 3459
Size: 1188 32 2239
Fragments arranged by size:
2239 1188 32
Tth111I GACn'n_nGTC
Cuts at: 0 674 816 3459
Size: 674 142 2643
Fragments arranged by size:
2643 674 142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 582 664 2094 3097 3459
Size: 582 82 1430 1003 362
Fragments arranged by size:
1430 1003 582 362 82
ZraI GAC'GTC
Cuts at: 0 2930 3459
Size: 2930 529
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BciVI BclI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrDI BsrFI BsrGI BstAPI BstEII BstYI Bsu36I
BtgI BtsI CspCI DraIII EaeI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI HaeII Hin4I HincII
HindIII HpaI MfeI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PciI PflMI PpuMI PshAI
PsiI PspOMI PstI PvuII SacI SanDI SexAI SfcI
SmlI SphI SspI StyI TaqII TatI TstI Tth111I
XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AloI AscI
AseI AsiSI BaeI BbeI BcgI BfrBI BglII BmgBI
BmtI BsaAI BsaBI BsiEI BsiWI BspHI BsrBI BssHII
BssSI BstBI BstXI BstZ17I BtgZI ClaI DraI DrdI
EagI EarI EciI FspI FspAI KasI KpnI MluI
NarI NdeI NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI XbaI XhoI
XmaI XmnI