(Linear) (Six-base) MAPSORT of: KIAA1820.seq Check: 3730 from: 1 to: 4920 KIAA1820.orf With 182 enzymes: SgfI * February 28, 2007 11:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2455 4680 4920 Size: 2455 2225 240 Fragments arranged by size: 2455 2225 240 Acc65I G'GTAC_C Cuts at: 0 616 2125 4920 Size: 616 1509 2795 Fragments arranged by size: 2795 1509 616 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 862 996 1234 1901 2028 4000 4920 Size: 337 525 134 238 667 127 1972 920 Fragments arranged by size: 1972 920 667 525 337 238 134 127 AfeI AGC'GCT Cuts at: 0 3424 4920 Size: 3424 1496 AflIII A'CryG_T Cuts at: 0 1688 4920 Size: 1688 3232 AgeI A'CCGG_T Cuts at: 0 2504 4920 Size: 2504 2416 AhdI GACnn_n'nnGTC Cuts at: 0 1689 2108 2670 4920 Size: 1689 419 562 2250 Fragments arranged by size: 2250 1689 562 419 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 268 4920 Size: 268 4652 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1390 1422 4920 Size: 1390 32 3498 Fragments arranged by size: 3498 1390 32 AlwNI CAG_nnn'CTG Cuts at: 0 132 546 576 1353 1356 1943 4544 4695 Size: 132 414 30 777 3 587 2601 151 Cuts at: 4695 4717 4920 Size: 22 203 Fragments arranged by size: 2601 777 587 414 203 151 132 30 22 3 ApaI G_GGCC'C Cuts at: 0 369 699 2788 3098 4365 4920 Size: 369 330 2089 310 1267 555 Fragments arranged by size: 2089 1267 555 369 330 310 ApaLI G'TGCA_C Cuts at: 0 3007 4869 4920 Size: 3007 1862 51 Fragments arranged by size: 3007 1862 51 AvaI C'yCGr_G Cuts at: 0 168 774 1648 2419 2485 2641 2944 3022 Size: 168 606 874 771 66 156 303 78 Cuts at: 3022 3917 4430 4622 4920 Size: 895 513 192 298 Fragments arranged by size: 895 874 771 606 513 303 298 192 168 156 78 66 BamHI G'GATC_C Cuts at: 0 4704 4920 Size: 4704 216 BanI G'GyrC_C Cuts at: 0 481 489 616 887 2125 2797 2949 3048 Size: 481 8 127 271 1238 672 152 99 Cuts at: 3048 3687 4156 4326 4920 Size: 639 469 170 594 Fragments arranged by size: 1238 672 639 594 481 469 271 170 152 127 99 8 BanII G_rGCy'C Cuts at: 0 369 699 1475 2630 2746 2788 2883 3098 Size: 369 330 776 1155 116 42 95 215 Cuts at: 3098 3129 3155 3197 3376 4075 4103 4365 4799 Size: 31 26 42 179 699 28 262 434 Cuts at: 4799 4920 Size: 121 Fragments arranged by size: 1155 776 699 434 369 330 262 215 179 121 116 95 42 42 31 28 26 BbeI G_GCGC'C Cuts at: 0 485 2953 3691 4330 4920 Size: 485 2468 738 639 590 Fragments arranged by size: 2468 738 639 590 485 BbsI GAAGACnn'nnnn_ Cuts at: 0 2069 3812 3949 4247 4920 Size: 2069 1743 137 298 673 Fragments arranged by size: 2069 1743 673 298 137 BbvCI CC'TCA_GC Cuts at: 0 629 820 2600 4756 4920 Size: 629 191 1780 2156 164 Fragments arranged by size: 2156 1780 629 191 164 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 26 4850 4884 4920 Size: 26 4824 34 36 Fragments arranged by size: 4824 36 34 26 BglI GCCn_nnn'nGGC Cuts at: 0 217 1727 2914 3371 3685 3919 4349 4920 Size: 217 1510 1187 457 314 234 430 571 Fragments arranged by size: 1510 1187 571 457 430 314 234 217 BglII A'GATC_T Cuts at: 0 3461 4920 Size: 3461 1459 BlpI GC'TnA_GC Cuts at: 0 112 1129 1795 2208 4920 Size: 112 1017 666 413 2712 Fragments arranged by size: 2712 1017 666 413 112 Bme1580I G_kGCm'C Cuts at: 0 369 455 494 699 2485 2788 2800 2945 Size: 369 86 39 205 1786 303 12 145 Cuts at: 2945 3011 3051 3098 4159 4365 4566 4873 4920 Size: 66 40 47 1061 206 201 307 47 Fragments arranged by size: 1786 1061 369 307 303 206 205 201 145 86 66 47 47 40 39 12 BmgBI CAC'GTC Cuts at: 0 516 4920 Size: 516 4404 BmrI ACTGGGnnnn_n' Cuts at: 0 633 3259 4117 4920 Size: 633 2626 858 803 Fragments arranged by size: 2626 858 803 633 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1350 2628 2631 3411 4488 4920 Size: 1350 1278 3 780 1077 432 Fragments arranged by size: 1350 1278 1077 780 432 3 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 747 1008 1120 1594 1948 2138 2326 2533 Size: 747 261 112 474 354 190 188 207 Cuts at: 2533 2867 3143 3303 3552 4408 4813 4920 Size: 334 276 160 249 856 405 107 Fragments arranged by size: 856 747 474 405 354 334 276 261 249 207 190 188 160 112 107 Bpu10I CC'TnA_GC Cuts at: 0 629 820 1552 2600 4756 4920 Size: 629 191 732 1048 2156 164 Fragments arranged by size: 2156 1048 732 629 191 164 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3889 4006 4087 4714 4920 Size: 3889 117 81 627 206 Fragments arranged by size: 3889 627 206 117 81 BsaI GGTCTCn'nnnn_ Cuts at: 0 3570 3880 4920 Size: 3570 310 1040 Fragments arranged by size: 3570 1040 310 BsaHI Gr'CG_yC Cuts at: 0 302 482 2906 2950 3688 4327 4920 Size: 302 180 2424 44 738 639 593 Fragments arranged by size: 2424 738 639 593 302 180 44 BsaWI w'CCGG_w Cuts at: 0 983 2504 4920 Size: 983 1521 2416 Fragments arranged by size: 2416 1521 983 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 577 607 1735 1765 1807 1837 3409 3439 Size: 577 30 1128 30 42 30 1572 30 Cuts at: 3439 4920 Size: 1481 Fragments arranged by size: 1572 1481 1128 577 42 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 358 645 657 660 2398 2452 2473 2604 Size: 358 287 12 3 1738 54 21 131 Cuts at: 2604 2617 2620 2719 2728 3520 3793 4353 4496 Size: 13 3 99 9 792 273 560 143 Cuts at: 4496 4519 4920 Size: 23 401 Fragments arranged by size: 1738 792 560 401 358 287 273 143 131 99 54 23 21 13 12 9 3 3 BseYI C'CCAG_C Cuts at: 0 457 536 731 1018 1888 2738 2781 3325 Size: 457 79 195 287 870 850 43 544 Cuts at: 3325 3400 4015 4111 4509 4523 4798 4920 Size: 75 615 96 398 14 275 122 Fragments arranged by size: 870 850 615 544 457 398 287 275 195 122 96 79 75 43 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 496 2679 3072 3218 3647 4018 4883 4920 Size: 496 2183 393 146 429 371 865 37 Fragments arranged by size: 2183 865 496 429 393 371 146 37 BsiEI CG_ry'CG Cuts at: 0 123 3338 4864 4920 Size: 123 3215 1526 56 Fragments arranged by size: 3215 1526 123 56 BsiHKAI G_wGCw'C Cuts at: 0 2630 2883 3011 4873 4920 Size: 2630 253 128 1862 47 Fragments arranged by size: 2630 1862 253 128 47 BsmI GAATG_Cn' Cuts at: 0 633 4920 Size: 633 4287 Bsp1286I G_dGCh'C Cuts at: 0 369 455 494 699 1475 2485 2630 2746 Size: 369 86 39 205 776 1010 145 116 Cuts at: 2746 2788 2800 2883 2945 3011 3051 3098 3129 Size: 42 12 83 62 66 40 47 31 Cuts at: 3129 3155 3197 3376 4075 4103 4159 4365 4566 Size: 26 42 179 699 28 56 206 201 Cuts at: 4566 4799 4873 4920 Size: 233 74 47 Fragments arranged by size: 1010 776 699 369 233 206 205 201 179 145 116 86 83 74 66 62 56 47 47 42 42 40 39 31 28 26 12 BspEI T'CCGG_A Cuts at: 0 983 4920 Size: 983 3937 BspHI T'CATG_A Cuts at: 0 4235 4920 Size: 4235 685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 411 1283 2455 4680 4920 Size: 411 872 1172 2225 240 Fragments arranged by size: 2225 1172 872 411 240 BsrBI CCG'CTC Cuts at: 0 1043 1055 1128 1457 1556 1740 1773 4920 Size: 1043 12 73 329 99 184 33 3147 Fragments arranged by size: 3147 1043 329 184 99 73 33 12 BsrDI GCAATG_nn' Cuts at: 0 587 2037 3765 3858 4920 Size: 587 1450 1728 93 1062 Fragments arranged by size: 1728 1450 1062 587 93 BsrFI r'CCGG_y Cuts at: 0 122 282 884 2504 2522 2734 3497 3647 Size: 122 160 602 1620 18 212 763 150 Cuts at: 3647 3690 4920 Size: 43 1230 Fragments arranged by size: 1620 1230 763 602 212 160 150 122 43 18 BssSI C'ACGA_G Cuts at: 0 1402 4920 Size: 1402 3518 BstAPI GCAn_nnn'nTGC Cuts at: 0 132 270 576 727 1356 4544 4692 4695 Size: 132 138 306 151 629 3188 148 3 Cuts at: 4695 4920 Size: 225 Fragments arranged by size: 3188 629 306 225 151 148 138 132 3 BstXI CCAn_nnnn'nTGG Cuts at: 0 685 1218 1606 1761 2304 4920 Size: 685 533 388 155 543 2616 Fragments arranged by size: 2616 685 543 533 388 155 BstYI r'GATC_y Cuts at: 0 1284 1829 3461 4216 4704 4920 Size: 1284 545 1632 755 488 216 Fragments arranged by size: 1632 1284 755 545 488 216 Bsu36I CC'TnA_GG Cuts at: 0 3788 4920 Size: 3788 1132 BtgI C'CryG_G Cuts at: 0 30 212 1046 1605 1783 2072 2977 4095 Size: 30 182 834 559 178 289 905 1118 Cuts at: 4095 4365 4920 Size: 270 555 Fragments arranged by size: 1118 905 834 559 555 289 270 182 178 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2940 4920 Size: 2940 1980 BtsI GCAGTG_nn' Cuts at: 0 203 455 1437 2485 2526 2560 4920 Size: 203 252 982 1048 41 34 2360 Fragments arranged by size: 2360 1048 982 252 203 41 34 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 3735 3770 4077 4112 4458 4493 4920 Size: 3735 35 307 35 346 35 427 Fragments arranged by size: 3735 427 346 307 35 35 35 DrdI GACnn_nn'nnGTC Cuts at: 0 979 4920 Size: 979 3941 EaeI y'GGCC_r Cuts at: 0 218 413 1958 2236 2492 3335 4827 4920 Size: 218 195 1545 278 256 843 1492 93 Fragments arranged by size: 1545 1492 843 278 256 218 195 93 EagI C'GGCC_G Cuts at: 0 3335 4920 Size: 3335 1585 EarI CTCTTCn'nnn_ Cuts at: 0 2852 3811 4330 4653 4920 Size: 2852 959 519 323 267 Fragments arranged by size: 2852 959 519 323 267 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 3495 4714 4867 4920 Size: 3495 1219 153 53 Fragments arranged by size: 3495 1219 153 53 EcoICRI GAG'CTC Cuts at: 0 2628 2881 4920 Size: 2628 253 2039 Fragments arranged by size: 2628 2039 253 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 747 862 996 1008 1120 1234 1594 Size: 337 410 115 134 12 112 114 360 Cuts at: 1594 1901 1948 2028 2138 2326 2533 2867 3143 Size: 307 47 80 110 188 207 334 276 Cuts at: 3143 3303 3552 4000 4408 4813 4920 Size: 160 249 448 408 405 107 Fragments arranged by size: 448 410 408 405 360 337 334 307 276 249 207 188 160 134 115 114 112 110 107 80 47 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 1252 1807 1897 2308 3626 4920 Size: 1252 555 90 411 1318 1294 Fragments arranged by size: 1318 1294 1252 555 411 90 EcoO109I rG'GnC_Cy Cuts at: 0 249 365 366 695 1139 1412 1566 1815 Size: 249 116 1 329 444 273 154 249 Cuts at: 1815 1995 2301 2388 2553 2589 2664 2682 2785 Size: 180 306 87 165 36 75 18 103 Cuts at: 2785 2863 3094 3095 3140 3485 3612 3975 4049 Size: 78 231 1 45 345 127 363 74 Cuts at: 4049 4299 4361 4541 4920 Size: 250 62 180 379 Fragments arranged by size: 444 379 363 345 329 306 273 250 249 249 231 180 180 165 154 127 116 103 87 78 75 74 62 45 36 18 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1935 3696 4920 Size: 1935 1761 1224 Fragments arranged by size: 1935 1761 1224 HaeII r_GCGC'y Cuts at: 0 188 485 1312 1802 1943 2222 2953 3426 Size: 188 297 827 490 141 279 731 473 Cuts at: 3426 3691 4045 4330 4617 4920 Size: 265 354 285 287 303 Fragments arranged by size: 827 731 490 473 354 303 297 287 285 279 265 188 141 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 577 609 1642 1674 1675 1707 1735 1767 Size: 577 32 1033 32 1 32 28 32 Cuts at: 1767 1807 1839 2094 2126 2656 2688 3153 3185 Size: 40 32 255 32 530 32 465 32 Cuts at: 3185 3409 3441 3807 3839 4920 Size: 224 32 366 32 1081 Fragments arranged by size: 1081 1033 577 530 465 366 255 224 40 32 32 32 32 32 32 32 32 32 32 28 1 HincII GTy'rAC Cuts at: 0 1626 4920 Size: 1626 3294 HindIII A'AGCT_T Cuts at: 0 1871 2988 4920 Size: 1871 1117 1932 Fragments arranged by size: 1932 1871 1117 KasI G'GCGC_C Cuts at: 0 481 2949 3687 4326 4920 Size: 481 2468 738 639 594 Fragments arranged by size: 2468 738 639 594 481 KpnI G_GTAC'C Cuts at: 0 620 2129 4920 Size: 620 1509 2791 Fragments arranged by size: 2791 1509 620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1262 1361 3380 4109 4231 4920 Size: 1262 99 2019 729 122 689 Fragments arranged by size: 2019 1262 729 689 122 99 MscI TGG'CCA Cuts at: 0 415 1960 2238 2494 4920 Size: 415 1545 278 256 2426 Fragments arranged by size: 2426 1545 415 278 256 MspA1I CmG'CkG Cuts at: 0 129 214 750 783 1086 1353 1488 1785 Size: 129 85 536 33 303 267 135 297 Cuts at: 1785 2016 2200 2212 2670 3603 4386 4920 Size: 231 184 12 458 933 783 534 Fragments arranged by size: 933 783 536 534 458 303 297 267 231 184 135 129 85 33 12 NaeI GCC'GGC Cuts at: 0 284 886 2524 2736 3499 4920 Size: 284 602 1638 212 763 1421 Fragments arranged by size: 1638 1421 763 602 284 212 NarI GG'CG_CC Cuts at: 0 482 2950 3688 4327 4920 Size: 482 2468 738 639 593 Fragments arranged by size: 2468 738 639 593 482 NcoI C'CATG_G Cuts at: 0 30 4365 4920 Size: 30 4335 555 Fragments arranged by size: 4335 555 30 NgoMIV G'CCGG_C Cuts at: 0 282 884 2522 2734 3497 4920 Size: 282 602 1638 212 763 1423 Fragments arranged by size: 1638 1423 763 602 282 212 NspI r_CATG'y Cuts at: 0 1528 1692 2661 4037 4920 Size: 1528 164 969 1376 883 Fragments arranged by size: 1528 1376 969 883 164 PasI CC'CwG_GG Cuts at: 0 612 2050 2395 3102 4920 Size: 612 1438 345 707 1818 Fragments arranged by size: 1818 1438 707 612 345 PciI A'CATG_T Cuts at: 0 1688 4920 Size: 1688 3232 PflMI CCAn_nnn'nTGG Cuts at: 0 4180 4920 Size: 4180 740 PfoI T'CCnGG_A Cuts at: 0 305 1408 2186 2378 4920 Size: 305 1103 778 192 2542 Fragments arranged by size: 2542 1103 778 305 192 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 4630 4662 4920 Size: 4630 32 258 Fragments arranged by size: 4630 258 32 PpuMI rG'GwC_Cy Cuts at: 0 1412 1566 1815 2388 2553 2589 2664 3612 Size: 1412 154 249 573 165 36 75 948 Cuts at: 3612 4920 Size: 1308 Fragments arranged by size: 1412 1308 948 573 249 165 154 75 36 PsiI TTA'TAA Cuts at: 0 152 4920 Size: 152 4768 PspOMI G'GGCC_C Cuts at: 0 365 695 2784 3094 4361 4920 Size: 365 330 2089 310 1267 559 Fragments arranged by size: 2089 1267 559 365 330 310 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1096 1128 4920 Size: 1096 32 3792 Fragments arranged by size: 3792 1096 32 PstI C_TGCA'G Cuts at: 0 210 275 408 581 1298 1444 1844 2480 Size: 210 65 133 173 717 146 400 636 Cuts at: 2480 4307 4920 Size: 1827 613 Fragments arranged by size: 1827 717 636 613 400 210 173 146 133 65 PvuII CAG'CTG Cuts at: 0 1353 2016 4920 Size: 1353 663 2904 Fragments arranged by size: 2904 1353 663 SacI G_AGCT'C Cuts at: 0 2630 2883 4920 Size: 2630 253 2037 Fragments arranged by size: 2630 2037 253 SacII CC_GC'GG Cuts at: 0 215 1786 4920 Size: 215 1571 3134 Fragments arranged by size: 3134 1571 215 SanDI GG'GwC_CC Cuts at: 0 3612 4920 Size: 3612 1308 SbfI CC_TGCA'GG Cuts at: 0 275 408 4920 Size: 275 133 4512 Fragments arranged by size: 4512 275 133 ScaI AGT'ACT Cuts at: 0 1000 2611 4920 Size: 1000 1611 2309 Fragments arranged by size: 2309 1611 1000 SexAI A'CCwGG_T Cuts at: 0 3621 3713 4920 Size: 3621 92 1207 Fragments arranged by size: 3621 1207 92 SfcI C'TryA_G Cuts at: 0 206 271 404 577 1065 1113 1294 1440 Size: 206 65 133 173 488 48 181 146 Cuts at: 1440 1840 2165 2476 2789 4303 4590 4920 Size: 400 325 311 313 1514 287 330 Fragments arranged by size: 1514 488 400 330 325 313 311 287 206 181 173 146 133 65 48 SfoI GGC'GCC Cuts at: 0 483 2951 3689 4328 4920 Size: 483 2468 738 639 592 Fragments arranged by size: 2468 738 639 592 483 SmaI CCC'GGG Cuts at: 0 776 2421 4920 Size: 776 1645 2499 Fragments arranged by size: 2499 1645 776 SmlI C'TyrA_G Cuts at: 0 3904 4021 4102 4729 4920 Size: 3904 117 81 627 191 Fragments arranged by size: 3904 627 191 117 81 SphI G_CATG'C Cuts at: 0 1528 2661 4920 Size: 1528 1133 2259 Fragments arranged by size: 2259 1528 1133 StuI AGG'CCT Cuts at: 0 269 2323 2652 3218 3786 4142 4282 4920 Size: 269 2054 329 566 568 356 140 638 Fragments arranged by size: 2054 638 568 566 356 329 269 140 StyI C'CwwG_G Cuts at: 0 30 140 1364 2036 2139 2297 2495 2678 Size: 30 110 1224 672 103 158 198 183 Cuts at: 2678 2699 2729 3308 3383 3845 3947 4365 4920 Size: 21 30 579 75 462 102 418 555 Fragments arranged by size: 1224 672 579 555 462 418 198 183 158 110 103 102 75 30 30 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1240 1902 2365 2434 2586 2691 3321 3414 Size: 1240 662 463 69 152 105 630 93 Cuts at: 3414 3428 4920 Size: 14 1492 Fragments arranged by size: 1492 1240 662 630 463 152 105 93 69 14 TatI w'GTAC_w Cuts at: 0 998 2609 3166 4733 4920 Size: 998 1611 557 1567 187 Fragments arranged by size: 1611 1567 998 557 187 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2518 2550 4920 Size: 2518 32 2370 Fragments arranged by size: 2518 2370 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 765 1215 2706 4383 4920 Size: 765 450 1491 1677 537 Fragments arranged by size: 1677 1491 765 537 450 XmaI C'CCGG_G Cuts at: 0 774 2419 4920 Size: 774 1645 2501 Fragments arranged by size: 2501 1645 774 Enzymes that do cut: AarI Acc65I AcuI AfeI AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI PsiI PspOMI PsrI PstI PvuII SacI SacII SanDI SbfI ScaI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII AccI AclI AflII AleI ApoI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BmtI BsaAI BsaBI BsiWI BsmBI BsrGI BssHII BstBI BstEII BstZ17I ClaI DraI DraIII EcoRI EcoRV FseI FspI FspAI HpaI MfeI MluI MslI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PshAI PspXI PvuI RsrII SalI SapI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI