Restriction map for pF1KSDA1820
 (Linear) (Six-base) MAPSORT of: KIAA1820.seq  Check: 3730  from: 1  to: 4920



KIAA1820.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2455    4680    4920 
   Size:       2455    2225     240

  Fragments arranged by size: 

                2455    2225     240
 
Acc65I G'GTAC_C

Cuts at:      0     616    2125    4920 
   Size:        616    1509    2795

  Fragments arranged by size: 

                2795    1509     616
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     862     996    1234    1901    2028    4000    4920 
   Size:        337     525     134     238     667     127    1972     920

  Fragments arranged by size: 

                1972     920     667     525     337     238     134     127
            
 
AfeI AGC'GCT

Cuts at:      0    3424    4920 
   Size:       3424    1496
 
AflIII A'CryG_T

Cuts at:      0    1688    4920 
   Size:       1688    3232
 
AgeI A'CCGG_T

Cuts at:      0    2504    4920 
   Size:       2504    2416
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1689    2108    2670    4920 
   Size:       1689     419     562    2250

  Fragments arranged by size: 

                2250    1689     562     419
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     268    4920 
   Size:        268    4652
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1390    1422    4920 
   Size:       1390      32    3498

  Fragments arranged by size: 

                3498    1390      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     132     546     576    1353    1356    1943    4544    4695 
   Size:        132     414      30     777       3     587    2601     151

Cuts at:   4695    4717    4920 
   Size:         22     203

  Fragments arranged by size: 

                2601     777     587     414     203     151     132      30
                  22       3
 
ApaI G_GGCC'C

Cuts at:      0     369     699    2788    3098    4365    4920 
   Size:        369     330    2089     310    1267     555

  Fragments arranged by size: 

                2089    1267     555     369     330     310
 
ApaLI G'TGCA_C

Cuts at:      0    3007    4869    4920 
   Size:       3007    1862      51

  Fragments arranged by size: 

                3007    1862      51
 
AvaI C'yCGr_G

Cuts at:      0     168     774    1648    2419    2485    2641    2944    3022 
   Size:        168     606     874     771      66     156     303      78

Cuts at:   3022    3917    4430    4622    4920 
   Size:        895     513     192     298

  Fragments arranged by size: 

                 895     874     771     606     513     303     298     192
                 168     156      78      66
 
BamHI G'GATC_C

Cuts at:      0    4704    4920 
   Size:       4704     216
 
BanI G'GyrC_C

Cuts at:      0     481     489     616     887    2125    2797    2949    3048 
   Size:        481       8     127     271    1238     672     152      99

Cuts at:   3048    3687    4156    4326    4920 
   Size:        639     469     170     594

  Fragments arranged by size: 

                1238     672     639     594     481     469     271     170
                 152     127      99       8
 
BanII G_rGCy'C

Cuts at:      0     369     699    1475    2630    2746    2788    2883    3098 
   Size:        369     330     776    1155     116      42      95     215

Cuts at:   3098    3129    3155    3197    3376    4075    4103    4365    4799 
   Size:         31      26      42     179     699      28     262     434

Cuts at:   4799    4920 
   Size:        121

  Fragments arranged by size: 

                1155     776     699     434     369     330     262     215
                 179     121     116      95      42      42      31      28
                  26
 
BbeI G_GCGC'C

Cuts at:      0     485    2953    3691    4330    4920 
   Size:        485    2468     738     639     590

  Fragments arranged by size: 

                2468     738     639     590     485
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2069    3812    3949    4247    4920 
   Size:       2069    1743     137     298     673

  Fragments arranged by size: 

                2069    1743     673     298     137
 
BbvCI CC'TCA_GC

Cuts at:      0     629     820    2600    4756    4920 
   Size:        629     191    1780    2156     164

  Fragments arranged by size: 

                2156    1780     629     191     164
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      26    4850    4884    4920 
   Size:         26    4824      34      36

  Fragments arranged by size: 

                4824      36      34      26
 
BglI GCCn_nnn'nGGC

Cuts at:      0     217    1727    2914    3371    3685    3919    4349    4920 
   Size:        217    1510    1187     457     314     234     430     571

  Fragments arranged by size: 

                1510    1187     571     457     430     314     234     217
            
 
BglII A'GATC_T

Cuts at:      0    3461    4920 
   Size:       3461    1459
 
BlpI GC'TnA_GC

Cuts at:      0     112    1129    1795    2208    4920 
   Size:        112    1017     666     413    2712

  Fragments arranged by size: 

                2712    1017     666     413     112
 
Bme1580I G_kGCm'C

Cuts at:      0     369     455     494     699    2485    2788    2800    2945 
   Size:        369      86      39     205    1786     303      12     145

Cuts at:   2945    3011    3051    3098    4159    4365    4566    4873    4920 
   Size:         66      40      47    1061     206     201     307      47

  Fragments arranged by size: 

                1786    1061     369     307     303     206     205     201
                 145      86      66      47      47      40      39      12
            
 
BmgBI CAC'GTC

Cuts at:      0     516    4920 
   Size:        516    4404
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     633    3259    4117    4920 
   Size:        633    2626     858     803

  Fragments arranged by size: 

                2626     858     803     633
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1350    2628    2631    3411    4488    4920 
   Size:       1350    1278       3     780    1077     432

  Fragments arranged by size: 

                1350    1278    1077     780     432       3
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     747    1008    1120    1594    1948    2138    2326    2533 
   Size:        747     261     112     474     354     190     188     207

Cuts at:   2533    2867    3143    3303    3552    4408    4813    4920 
   Size:        334     276     160     249     856     405     107

  Fragments arranged by size: 

                 856     747     474     405     354     334     276     261
                 249     207     190     188     160     112     107
 
Bpu10I CC'TnA_GC

Cuts at:      0     629     820    1552    2600    4756    4920 
   Size:        629     191     732    1048    2156     164

  Fragments arranged by size: 

                2156    1048     732     629     191     164
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3889    4006    4087    4714    4920 
   Size:       3889     117      81     627     206

  Fragments arranged by size: 

                3889     627     206     117      81
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3570    3880    4920 
   Size:       3570     310    1040

  Fragments arranged by size: 

                3570    1040     310
 
BsaHI Gr'CG_yC

Cuts at:      0     302     482    2906    2950    3688    4327    4920 
   Size:        302     180    2424      44     738     639     593

  Fragments arranged by size: 

                2424     738     639     593     302     180      44
 
BsaWI w'CCGG_w

Cuts at:      0     983    2504    4920 
   Size:        983    1521    2416

  Fragments arranged by size: 

                2416    1521     983
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     577     607    1735    1765    1807    1837    3409    3439 
   Size:        577      30    1128      30      42      30    1572      30

Cuts at:   3439    4920 
   Size:       1481

  Fragments arranged by size: 

                1572    1481    1128     577      42      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     358     645     657     660    2398    2452    2473    2604 
   Size:        358     287      12       3    1738      54      21     131

Cuts at:   2604    2617    2620    2719    2728    3520    3793    4353    4496 
   Size:         13       3      99       9     792     273     560     143

Cuts at:   4496    4519    4920 
   Size:         23     401

  Fragments arranged by size: 

                1738     792     560     401     358     287     273     143
                 131      99      54      23      21      13      12       9
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0     457     536     731    1018    1888    2738    2781    3325 
   Size:        457      79     195     287     870     850      43     544

Cuts at:   3325    3400    4015    4111    4509    4523    4798    4920 
   Size:         75     615      96     398      14     275     122

  Fragments arranged by size: 

                 870     850     615     544     457     398     287     275
                 195     122      96      79      75      43      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496    2679    3072    3218    3647    4018    4883    4920 
   Size:        496    2183     393     146     429     371     865      37

  Fragments arranged by size: 

                2183     865     496     429     393     371     146      37
            
 
BsiEI CG_ry'CG

Cuts at:      0     123    3338    4864    4920 
   Size:        123    3215    1526      56

  Fragments arranged by size: 

                3215    1526     123      56
 
BsiHKAI G_wGCw'C

Cuts at:      0    2630    2883    3011    4873    4920 
   Size:       2630     253     128    1862      47

  Fragments arranged by size: 

                2630    1862     253     128      47
 
BsmI GAATG_Cn'

Cuts at:      0     633    4920 
   Size:        633    4287
 
Bsp1286I G_dGCh'C

Cuts at:      0     369     455     494     699    1475    2485    2630    2746 
   Size:        369      86      39     205     776    1010     145     116

Cuts at:   2746    2788    2800    2883    2945    3011    3051    3098    3129 
   Size:         42      12      83      62      66      40      47      31

Cuts at:   3129    3155    3197    3376    4075    4103    4159    4365    4566 
   Size:         26      42     179     699      28      56     206     201

Cuts at:   4566    4799    4873    4920 
   Size:        233      74      47

  Fragments arranged by size: 

                1010     776     699     369     233     206     205     201
                 179     145     116      86      83      74      66      62
                  56      47      47      42      42      40      39      31
                  28      26      12
 
BspEI T'CCGG_A

Cuts at:      0     983    4920 
   Size:        983    3937
 
BspHI T'CATG_A

Cuts at:      0    4235    4920 
   Size:       4235     685
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     411    1283    2455    4680    4920 
   Size:        411     872    1172    2225     240

  Fragments arranged by size: 

                2225    1172     872     411     240
 
BsrBI CCG'CTC

Cuts at:      0    1043    1055    1128    1457    1556    1740    1773    4920 
   Size:       1043      12      73     329      99     184      33    3147

  Fragments arranged by size: 

                3147    1043     329     184      99      73      33      12
            
 
BsrDI GCAATG_nn'

Cuts at:      0     587    2037    3765    3858    4920 
   Size:        587    1450    1728      93    1062

  Fragments arranged by size: 

                1728    1450    1062     587      93
 
BsrFI r'CCGG_y

Cuts at:      0     122     282     884    2504    2522    2734    3497    3647 
   Size:        122     160     602    1620      18     212     763     150

Cuts at:   3647    3690    4920 
   Size:         43    1230

  Fragments arranged by size: 

                1620    1230     763     602     212     160     150     122
                  43      18
 
BssSI C'ACGA_G

Cuts at:      0    1402    4920 
   Size:       1402    3518
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     132     270     576     727    1356    4544    4692    4695 
   Size:        132     138     306     151     629    3188     148       3

Cuts at:   4695    4920 
   Size:        225

  Fragments arranged by size: 

                3188     629     306     225     151     148     138     132
                   3
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     685    1218    1606    1761    2304    4920 
   Size:        685     533     388     155     543    2616

  Fragments arranged by size: 

                2616     685     543     533     388     155
 
BstYI r'GATC_y

Cuts at:      0    1284    1829    3461    4216    4704    4920 
   Size:       1284     545    1632     755     488     216

  Fragments arranged by size: 

                1632    1284     755     545     488     216
 
Bsu36I CC'TnA_GG

Cuts at:      0    3788    4920 
   Size:       3788    1132
 
BtgI C'CryG_G

Cuts at:      0      30     212    1046    1605    1783    2072    2977    4095 
   Size:         30     182     834     559     178     289     905    1118

Cuts at:   4095    4365    4920 
   Size:        270     555

  Fragments arranged by size: 

                1118     905     834     559     555     289     270     182
                 178      30
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2940    4920 
   Size:       2940    1980
 
BtsI GCAGTG_nn'

Cuts at:      0     203     455    1437    2485    2526    2560    4920 
   Size:        203     252     982    1048      41      34    2360

  Fragments arranged by size: 

                2360    1048     982     252     203      41      34
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    3735    3770    4077    4112    4458    4493    4920 
   Size:       3735      35     307      35     346      35     427

  Fragments arranged by size: 

                3735     427     346     307      35      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     979    4920 
   Size:        979    3941
 
EaeI y'GGCC_r

Cuts at:      0     218     413    1958    2236    2492    3335    4827    4920 
   Size:        218     195    1545     278     256     843    1492      93

  Fragments arranged by size: 

                1545    1492     843     278     256     218     195      93
            
 
EagI C'GGCC_G

Cuts at:      0    3335    4920 
   Size:       3335    1585
 
EarI CTCTTCn'nnn_

Cuts at:      0    2852    3811    4330    4653    4920 
   Size:       2852     959     519     323     267

  Fragments arranged by size: 

                2852     959     519     323     267
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3495    4714    4867    4920 
   Size:       3495    1219     153      53

  Fragments arranged by size: 

                3495    1219     153      53
 
EcoICRI GAG'CTC

Cuts at:      0    2628    2881    4920 
   Size:       2628     253    2039

  Fragments arranged by size: 

                2628    2039     253
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     747     862     996    1008    1120    1234    1594 
   Size:        337     410     115     134      12     112     114     360

Cuts at:   1594    1901    1948    2028    2138    2326    2533    2867    3143 
   Size:        307      47      80     110     188     207     334     276

Cuts at:   3143    3303    3552    4000    4408    4813    4920 
   Size:        160     249     448     408     405     107

  Fragments arranged by size: 

                 448     410     408     405     360     337     334     307
                 276     249     207     188     160     134     115     114
                 112     110     107      80      47      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1252    1807    1897    2308    3626    4920 
   Size:       1252     555      90     411    1318    1294

  Fragments arranged by size: 

                1318    1294    1252     555     411      90
 
EcoO109I rG'GnC_Cy

Cuts at:      0     249     365     366     695    1139    1412    1566    1815 
   Size:        249     116       1     329     444     273     154     249

Cuts at:   1815    1995    2301    2388    2553    2589    2664    2682    2785 
   Size:        180     306      87     165      36      75      18     103

Cuts at:   2785    2863    3094    3095    3140    3485    3612    3975    4049 
   Size:         78     231       1      45     345     127     363      74

Cuts at:   4049    4299    4361    4541    4920 
   Size:        250      62     180     379

  Fragments arranged by size: 

                 444     379     363     345     329     306     273     250
                 249     249     231     180     180     165     154     127
                 116     103      87      78      75      74      62      45
                  36      18       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1935    3696    4920 
   Size:       1935    1761    1224

  Fragments arranged by size: 

                1935    1761    1224
 
HaeII r_GCGC'y

Cuts at:      0     188     485    1312    1802    1943    2222    2953    3426 
   Size:        188     297     827     490     141     279     731     473

Cuts at:   3426    3691    4045    4330    4617    4920 
   Size:        265     354     285     287     303

  Fragments arranged by size: 

                 827     731     490     473     354     303     297     287
                 285     279     265     188     141
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     577     609    1642    1674    1675    1707    1735    1767 
   Size:        577      32    1033      32       1      32      28      32

Cuts at:   1767    1807    1839    2094    2126    2656    2688    3153    3185 
   Size:         40      32     255      32     530      32     465      32

Cuts at:   3185    3409    3441    3807    3839    4920 
   Size:        224      32     366      32    1081

  Fragments arranged by size: 

                1081    1033     577     530     465     366     255     224
                  40      32      32      32      32      32      32      32
                  32      32      32      28       1
 
HincII GTy'rAC

Cuts at:      0    1626    4920 
   Size:       1626    3294
 
HindIII A'AGCT_T

Cuts at:      0    1871    2988    4920 
   Size:       1871    1117    1932

  Fragments arranged by size: 

                1932    1871    1117
 
KasI G'GCGC_C

Cuts at:      0     481    2949    3687    4326    4920 
   Size:        481    2468     738     639     594

  Fragments arranged by size: 

                2468     738     639     594     481
 
KpnI G_GTAC'C

Cuts at:      0     620    2129    4920 
   Size:        620    1509    2791

  Fragments arranged by size: 

                2791    1509     620
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1262    1361    3380    4109    4231    4920 
   Size:       1262      99    2019     729     122     689

  Fragments arranged by size: 

                2019    1262     729     689     122      99
 
MscI TGG'CCA

Cuts at:      0     415    1960    2238    2494    4920 
   Size:        415    1545     278     256    2426

  Fragments arranged by size: 

                2426    1545     415     278     256
 
MspA1I CmG'CkG

Cuts at:      0     129     214     750     783    1086    1353    1488    1785 
   Size:        129      85     536      33     303     267     135     297

Cuts at:   1785    2016    2200    2212    2670    3603    4386    4920 
   Size:        231     184      12     458     933     783     534

  Fragments arranged by size: 

                 933     783     536     534     458     303     297     267
                 231     184     135     129      85      33      12
 
NaeI GCC'GGC

Cuts at:      0     284     886    2524    2736    3499    4920 
   Size:        284     602    1638     212     763    1421

  Fragments arranged by size: 

                1638    1421     763     602     284     212
 
NarI GG'CG_CC

Cuts at:      0     482    2950    3688    4327    4920 
   Size:        482    2468     738     639     593

  Fragments arranged by size: 

                2468     738     639     593     482
 
NcoI C'CATG_G

Cuts at:      0      30    4365    4920 
   Size:         30    4335     555

  Fragments arranged by size: 

                4335     555      30
 
NgoMIV G'CCGG_C

Cuts at:      0     282     884    2522    2734    3497    4920 
   Size:        282     602    1638     212     763    1423

  Fragments arranged by size: 

                1638    1423     763     602     282     212
 
NspI r_CATG'y

Cuts at:      0    1528    1692    2661    4037    4920 
   Size:       1528     164     969    1376     883

  Fragments arranged by size: 

                1528    1376     969     883     164
 
PasI CC'CwG_GG

Cuts at:      0     612    2050    2395    3102    4920 
   Size:        612    1438     345     707    1818

  Fragments arranged by size: 

                1818    1438     707     612     345
 
PciI A'CATG_T

Cuts at:      0    1688    4920 
   Size:       1688    3232
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    4180    4920 
   Size:       4180     740
 
PfoI T'CCnGG_A

Cuts at:      0     305    1408    2186    2378    4920 
   Size:        305    1103     778     192    2542

  Fragments arranged by size: 

                2542    1103     778     305     192
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    4630    4662    4920 
   Size:       4630      32     258

  Fragments arranged by size: 

                4630     258      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1412    1566    1815    2388    2553    2589    2664    3612 
   Size:       1412     154     249     573     165      36      75     948

Cuts at:   3612    4920 
   Size:       1308

  Fragments arranged by size: 

                1412    1308     948     573     249     165     154      75
                  36
 
PsiI TTA'TAA

Cuts at:      0     152    4920 
   Size:        152    4768
 
PspOMI G'GGCC_C

Cuts at:      0     365     695    2784    3094    4361    4920 
   Size:        365     330    2089     310    1267     559

  Fragments arranged by size: 

                2089    1267     559     365     330     310
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1096    1128    4920 
   Size:       1096      32    3792

  Fragments arranged by size: 

                3792    1096      32
 
PstI C_TGCA'G

Cuts at:      0     210     275     408     581    1298    1444    1844    2480 
   Size:        210      65     133     173     717     146     400     636

Cuts at:   2480    4307    4920 
   Size:       1827     613

  Fragments arranged by size: 

                1827     717     636     613     400     210     173     146
                 133      65
 
PvuII CAG'CTG

Cuts at:      0    1353    2016    4920 
   Size:       1353     663    2904

  Fragments arranged by size: 

                2904    1353     663
 
SacI G_AGCT'C

Cuts at:      0    2630    2883    4920 
   Size:       2630     253    2037

  Fragments arranged by size: 

                2630    2037     253
 
SacII CC_GC'GG

Cuts at:      0     215    1786    4920 
   Size:        215    1571    3134

  Fragments arranged by size: 

                3134    1571     215
 
SanDI GG'GwC_CC

Cuts at:      0    3612    4920 
   Size:       3612    1308
 
SbfI CC_TGCA'GG

Cuts at:      0     275     408    4920 
   Size:        275     133    4512

  Fragments arranged by size: 

                4512     275     133
 
ScaI AGT'ACT

Cuts at:      0    1000    2611    4920 
   Size:       1000    1611    2309

  Fragments arranged by size: 

                2309    1611    1000
 
SexAI A'CCwGG_T

Cuts at:      0    3621    3713    4920 
   Size:       3621      92    1207

  Fragments arranged by size: 

                3621    1207      92
 
SfcI C'TryA_G

Cuts at:      0     206     271     404     577    1065    1113    1294    1440 
   Size:        206      65     133     173     488      48     181     146

Cuts at:   1440    1840    2165    2476    2789    4303    4590    4920 
   Size:        400     325     311     313    1514     287     330

  Fragments arranged by size: 

                1514     488     400     330     325     313     311     287
                 206     181     173     146     133      65      48
 
SfoI GGC'GCC

Cuts at:      0     483    2951    3689    4328    4920 
   Size:        483    2468     738     639     592

  Fragments arranged by size: 

                2468     738     639     592     483
 
SmaI CCC'GGG

Cuts at:      0     776    2421    4920 
   Size:        776    1645    2499

  Fragments arranged by size: 

                2499    1645     776
 
SmlI C'TyrA_G

Cuts at:      0    3904    4021    4102    4729    4920 
   Size:       3904     117      81     627     191

  Fragments arranged by size: 

                3904     627     191     117      81
 
SphI G_CATG'C

Cuts at:      0    1528    2661    4920 
   Size:       1528    1133    2259

  Fragments arranged by size: 

                2259    1528    1133
 
StuI AGG'CCT

Cuts at:      0     269    2323    2652    3218    3786    4142    4282    4920 
   Size:        269    2054     329     566     568     356     140     638

  Fragments arranged by size: 

                2054     638     568     566     356     329     269     140
            
 
StyI C'CwwG_G

Cuts at:      0      30     140    1364    2036    2139    2297    2495    2678 
   Size:         30     110    1224     672     103     158     198     183

Cuts at:   2678    2699    2729    3308    3383    3845    3947    4365    4920 
   Size:         21      30     579      75     462     102     418     555

  Fragments arranged by size: 

                1224     672     579     555     462     418     198     183
                 158     110     103     102      75      30      30      21
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1240    1902    2365    2434    2586    2691    3321    3414 
   Size:       1240     662     463      69     152     105     630      93

Cuts at:   3414    3428    4920 
   Size:         14    1492

  Fragments arranged by size: 

                1492    1240     662     630     463     152     105      93
                  69      14
 
TatI w'GTAC_w

Cuts at:      0     998    2609    3166    4733    4920 
   Size:        998    1611     557    1567     187

  Fragments arranged by size: 

                1611    1567     998     557     187
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2518    2550    4920 
   Size:       2518      32    2370

  Fragments arranged by size: 

                2518    2370      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     765    1215    2706    4383    4920 
   Size:        765     450    1491    1677     537

  Fragments arranged by size: 

                1677    1491     765     537     450
 
XmaI C'CCGG_G

Cuts at:      0     774    2419    4920 
   Size:        774    1645    2501

  Fragments arranged by size: 

                2501    1645     774
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI     AlfI
     AloI    AlwNI     ApaI    ApaLI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PpiI    PpuMI     PsiI   PspOMI
     PsrI     PstI    PvuII     SacI    SacII    SanDI     SbfI     ScaI
    SexAI     SfcI     SfoI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII     AleI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BciVI     BclI    BfrBI     BmtI    BsaAI
    BsaBI    BsiWI    BsmBI    BsrGI   BssHII    BstBI   BstEII  BstZ17I
     ClaI     DraI   DraIII    EcoRI    EcoRV     FseI     FspI    FspAI
     HpaI     MfeI     MluI     MslI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PmeI     PmlI    PshAI    PspXI     PvuI    RsrII
     SalI     SapI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI  Tth111I     XbaI     XhoI     XmnI     ZraI
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