Restriction map for
pF1KA1824
(Linear) (Six-base) MAPSORT of: KIAA1824.seq Check: 6324 from: 1 to: 2826
KIAA1824.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 934 2826
Size: 934 1892
Acc65I G'GTAC_C
Cuts at: 0 2278 2826
Size: 2278 548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2195 2826
Size: 2195 631
AfeI AGC'GCT
Cuts at: 0 1357 2826
Size: 1357 1469
AflIII A'CryG_T
Cuts at: 0 410 914 1504 2826
Size: 410 504 590 1322
Fragments arranged by size:
1322 590 504 410
AhdI GACnn_n'nnGTC
Cuts at: 0 1377 2826
Size: 1377 1449
AleI CACnn'nnGTG
Cuts at: 0 343 1503 2826
Size: 343 1160 1323
Fragments arranged by size:
1323 1160 343
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1769 2826
Size: 1769 1057
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 242 274 2826
Size: 242 32 2552
Fragments arranged by size:
2552 242 32
AlwNI CAG_nnn'CTG
Cuts at: 0 204 744 1536 2166 2599 2826
Size: 204 540 792 630 433 227
Fragments arranged by size:
792 630 540 433 227 204
ApaI G_GGCC'C
Cuts at: 0 1454 2826
Size: 1454 1372
ApoI r'AATT_y
Cuts at: 0 178 923 1976 2796 2826
Size: 178 745 1053 820 30
Fragments arranged by size:
1053 820 745 178 30
AvaI C'yCGr_G
Cuts at: 0 1781 2826
Size: 1781 1045
BanI G'GyrC_C
Cuts at: 0 1763 2268 2278 2826
Size: 1763 505 10 548
Fragments arranged by size:
1763 548 505 10
BanII G_rGCy'C
Cuts at: 0 454 539 599 895 1084 1294 1454 2826
Size: 454 85 60 296 189 210 160 1372
Fragments arranged by size:
1372 454 296 210 189 160 85 60
BbeI G_GCGC'C
Cuts at: 0 1767 2826
Size: 1767 1059
BbsI GAAGACnn'nnnn_
Cuts at: 0 1238 1373 1780 1793 1841 2826
Size: 1238 135 407 13 48 985
Fragments arranged by size:
1238 985 407 135 48 13
BbvCI CC'TCA_GC
Cuts at: 0 2366 2826
Size: 2366 460
BglI GCCn_nnn'nGGC
Cuts at: 0 23 116 158 2750 2826
Size: 23 93 42 2592 76
Fragments arranged by size:
2592 93 76 42 23
BglII A'GATC_T
Cuts at: 0 1073 1632 2826
Size: 1073 559 1194
Fragments arranged by size:
1194 1073 559
Bme1580I G_kGCm'C
Cuts at: 0 1454 1803 2826
Size: 1454 349 1023
Fragments arranged by size:
1454 1023 349
BmgBI CAC'GTC
Cuts at: 0 411 2826
Size: 411 2415
BmrI ACTGGGnnnn_n'
Cuts at: 0 448 1790 2066 2439 2826
Size: 448 1342 276 373 387
Fragments arranged by size:
1342 448 387 373 276
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 655 2298 2817 2826
Size: 655 1643 519 9
Fragments arranged by size:
1643 655 519 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 374 1098 1127 1308 2581 2826
Size: 249 125 724 29 181 1273 245
Fragments arranged by size:
1273 724 249 245 181 125 29
Bpu10I CC'TnA_GC
Cuts at: 0 1359 1898 2353 2366 2826
Size: 1359 539 455 13 460
Fragments arranged by size:
1359 539 460 455 13
BsaI GGTCTCn'nnnn_
Cuts at: 0 213 1103 2035 2665 2826
Size: 213 890 932 630 161
Fragments arranged by size:
932 890 630 213 161
BsaAI yAC'GTr
Cuts at: 0 1505 2826
Size: 1505 1321
BsaHI Gr'CG_yC
Cuts at: 0 108 759 1764 2826
Size: 108 651 1005 1062
Fragments arranged by size:
1062 1005 651 108
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 586 616 1861 1891 2826
Size: 586 30 1245 30 935
Fragments arranged by size:
1245 935 586 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 117 136 230 1615 1726 1729 1866
Size: 21 96 19 94 1385 111 3 137
Cuts at: 1866 1869 2372 2383 2476 2481 2793 2826
Size: 3 503 11 93 5 312 33
Fragments arranged by size:
1385 503 312 137 111 96 94 93
33 21 19 11 5 3 3
BseYI C'CCAG_C
Cuts at: 0 56 737 1342 1657 2103 2221 2712 2826
Size: 56 681 605 315 446 118 491 114
Fragments arranged by size:
681 605 491 446 315 118 114 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1321 2826
Size: 1321 1505
BsiEI CG_ry'CG
Cuts at: 0 1122 2826
Size: 1122 1704
BsiHKAI G_wGCw'C
Cuts at: 0 299 776 1294 1909 2246 2318 2826
Size: 299 477 518 615 337 72 508
Fragments arranged by size:
615 518 508 477 337 299 72
BsmI GAATG_Cn'
Cuts at: 0 1758 2826
Size: 1758 1068
BsmBI CGTCTCn'nnnn_
Cuts at: 0 99 2826
Size: 99 2727
Bsp1286I G_dGCh'C
Cuts at: 0 299 454 539 599 776 895 1084 1294
Size: 299 155 85 60 177 119 189 210
Cuts at: 1294 1454 1803 1909 2246 2318 2826
Size: 160 349 106 337 72 508
Fragments arranged by size:
508 349 337 299 210 189 177 160
155 119 106 85 72 60
BspHI T'CATG_A
Cuts at: 0 420 2826
Size: 420 2406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 934 2174 2826
Size: 934 1240 652
Fragments arranged by size:
1240 934 652
BsrBI CCG'CTC
Cuts at: 0 1902 2826
Size: 1902 924
BsrDI GCAATG_nn'
Cuts at: 0 366 1390 2150 2232 2826
Size: 366 1024 760 82 594
Fragments arranged by size:
1024 760 594 366 82
BsrGI T'GTAC_A
Cuts at: 0 911 2826
Size: 911 1915
BstBI TT'CG_AA
Cuts at: 0 651 2826
Size: 651 2175
BstEII G'GTnAC_C
Cuts at: 0 1461 1652 2826
Size: 1461 191 1174
Fragments arranged by size:
1461 1174 191
BstXI CCAn_nnnn'nTGG
Cuts at: 0 606 2500 2826
Size: 606 1894 326
Fragments arranged by size:
1894 606 326
BstYI r'GATC_y
Cuts at: 0 933 1073 1632 2826
Size: 933 140 559 1194
Fragments arranged by size:
1194 933 559 140
Bsu36I CC'TnA_GG
Cuts at: 0 751 2826
Size: 751 2075
BtgI C'CryG_G
Cuts at: 0 528 1648 2826
Size: 528 1120 1178
Fragments arranged by size:
1178 1120 528
BtsI GCAGTG_nn'
Cuts at: 0 402 2826
Size: 402 2424
ClaI AT'CG_AT
Cuts at: 0 996 2826
Size: 996 1830
DraI TTT'AAA
Cuts at: 0 2199 2826
Size: 2199 627
EaeI y'GGCC_r
Cuts at: 0 150 492 608 1645 2742 2826
Size: 150 342 116 1037 1097 84
Fragments arranged by size:
1097 1037 342 150 116 84
EarI CTCTTCn'nnn_
Cuts at: 0 1053 1154 2198 2583 2826
Size: 1053 101 1044 385 243
Fragments arranged by size:
1053 1044 385 243 101
EcoICRI GAG'CTC
Cuts at: 0 1292 2826
Size: 1292 1534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 374 1098 1127 1308 2195 2581 2826
Size: 249 125 724 29 181 887 386 245
Fragments arranged by size:
887 724 386 249 245 181 125 29
EcoO109I rG'GnC_Cy
Cuts at: 0 75 141 734 1450 1451 2163 2349 2441
Size: 75 66 593 716 1 712 186 92
Cuts at: 2441 2697 2826
Size: 256 129
Fragments arranged by size:
716 712 593 256 186 129 92 75
66 1
EcoRI G'AATT_C
Cuts at: 0 178 923 2796 2826
Size: 178 745 1873 30
Fragments arranged by size:
1873 745 178 30
HaeII r_GCGC'y
Cuts at: 0 1359 1554 1767 2826
Size: 1359 195 213 1059
Fragments arranged by size:
1359 1059 213 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1363 1395 1504 1536 1858 1861 1890 1893
Size: 1363 32 109 32 322 3 29 3
Cuts at: 1893 1945 1977 2599 2631 2826
Size: 52 32 622 32 195
Fragments arranged by size:
1363 622 322 195 109 52 32 32
32 32 29 3 3
HincII GTy'rAC
Cuts at: 0 966 1008 2826
Size: 966 42 1818
Fragments arranged by size:
1818 966 42
HindIII A'AGCT_T
Cuts at: 0 1985 2826
Size: 1985 841
HpaI GTT'AAC
Cuts at: 0 1008 2826
Size: 1008 1818
KasI G'GCGC_C
Cuts at: 0 1763 2826
Size: 1763 1063
KpnI G_GTAC'C
Cuts at: 0 2282 2826
Size: 2282 544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 329 1102 1356 2826
Size: 209 120 773 254 1470
Fragments arranged by size:
1470 773 254 209 120
MscI TGG'CCA
Cuts at: 0 610 2744 2826
Size: 610 2134 82
Fragments arranged by size:
2134 610 82
MslI CAynn'nnrTG
Cuts at: 0 343 425 1036 1503 2143 2498 2826
Size: 343 82 611 467 640 355 328
Fragments arranged by size:
640 611 467 355 343 328 82
MspA1I CmG'CkG
Cuts at: 0 60 285 1142 1749 2420 2826
Size: 60 225 857 607 671 406
Fragments arranged by size:
857 671 607 406 225 60
NarI GG'CG_CC
Cuts at: 0 1764 2826
Size: 1764 1062
NcoI C'CATG_G
Cuts at: 0 528 2826
Size: 528 2298
NspI r_CATG'y
Cuts at: 0 918 2826
Size: 918 1908
PciI A'CATG_T
Cuts at: 0 914 2826
Size: 914 1912
PflMI CCAn_nnn'nTGG
Cuts at: 0 204 744 2826
Size: 204 540 2082
Fragments arranged by size:
2082 540 204
PfoI T'CCnGG_A
Cuts at: 0 1226 2826
Size: 1226 1600
PmlI CAC'GTG
Cuts at: 0 1505 2826
Size: 1505 1321
PpuMI rG'GwC_Cy
Cuts at: 0 75 141 734 2163 2441 2697 2826
Size: 75 66 593 1429 278 256 129
Fragments arranged by size:
1429 593 278 256 129 75 66
PsiI TTA'TAA
Cuts at: 0 1982 2826
Size: 1982 844
PspOMI G'GGCC_C
Cuts at: 0 1450 2826
Size: 1450 1376
PstI C_TGCA'G
Cuts at: 0 124 933 2185 2826
Size: 124 809 1252 641
Fragments arranged by size:
1252 809 641 124
PvuII CAG'CTG
Cuts at: 0 285 2826
Size: 285 2541
SacI G_AGCT'C
Cuts at: 0 1294 2826
Size: 1294 1532
SanDI GG'GwC_CC
Cuts at: 0 75 734 2826
Size: 75 659 2092
Fragments arranged by size:
2092 659 75
SapI GCTCTTCn'nnn_
Cuts at: 0 1053 2826
Size: 1053 1773
ScaI AGT'ACT
Cuts at: 0 1323 2826
Size: 1323 1503
SfcI C'TryA_G
Cuts at: 0 120 929 2181 2309 2826
Size: 120 809 1252 128 517
Fragments arranged by size:
1252 809 517 128 120
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2750 2826
Size: 2750 76
SfoI GGC'GCC
Cuts at: 0 1765 2826
Size: 1765 1061
StyI C'CwwG_G
Cuts at: 0 528 2048 2393 2826
Size: 528 1520 345 433
Fragments arranged by size:
1520 528 433 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1212 1515 1671 2826
Size: 1212 303 156 1155
Fragments arranged by size:
1212 1155 303 156
TatI w'GTAC_w
Cuts at: 0 911 1102 1163 1321 2826
Size: 911 191 61 158 1505
Fragments arranged by size:
1505 911 191 158 61
Tth111I GACn'n_nGTC
Cuts at: 0 2445 2826
Size: 2445 381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1339 2826
Size: 1339 1487
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrGI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtsI ClaI DraI EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI NspI
PciI PflMI PfoI PmlI PpuMI PsiI PspOMI PstI
PvuII SacI SanDI SapI ScaI SfcI SfiI SfoI
StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BlpI BmtI BpuEI BsaBI BsaWI BsiWI BspEI BsrFI
BssHII BssSI BstAPI BstZ17I BtgZI CspCI DraIII DrdI
EagI EciI EcoNI EcoRV FalI FseI FspI FspAI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PpiI PshAI PspXI PsrI
PvuI RsrII SacII SalI SbfI SexAI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI XbaI XhoI XmaI XmnI ZraI