Restriction map for
pF1KSDA1831
(Linear) (Six-base) MAPSORT of: KIAA1831.seq Check: 4753 from: 1 to: 1884
KIAA1831.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AatII G_ACGT'C
Cuts at: 0 699 1884
Size: 699 1185
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1884
Size: 292 1592
AfeI AGC'GCT
Cuts at: 0 895 1884
Size: 895 989
AhdI GACnn_n'nnGTC
Cuts at: 0 615 1884
Size: 615 1269
AleI CACnn'nnGTG
Cuts at: 0 838 1884
Size: 838 1046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1054 1884
Size: 1054 830
AlwNI CAG_nnn'CTG
Cuts at: 0 122 1245 1884
Size: 122 1123 639
Fragments arranged by size:
1123 639 122
ApaI G_GGCC'C
Cuts at: 0 97 167 599 794 1574 1674 1884
Size: 97 70 432 195 780 100 210
Fragments arranged by size:
780 432 210 195 100 97 70
ApaLI G'TGCA_C
Cuts at: 0 87 1012 1884
Size: 87 925 872
Fragments arranged by size:
925 872 87
AvaI C'yCGr_G
Cuts at: 0 7 564 572 598 1728 1765 1884
Size: 7 557 8 26 1130 37 119
Fragments arranged by size:
1130 557 119 37 26 8 7
BamHI G'GATC_C
Cuts at: 0 1366 1884
Size: 1366 518
BanI G'GyrC_C
Cuts at: 0 100 379 1096 1748 1884
Size: 100 279 717 652 136
Fragments arranged by size:
717 652 279 136 100
BanII G_rGCy'C
Cuts at: 0 97 167 326 571 599 683 794 829
Size: 97 70 159 245 28 84 111 35
Cuts at: 829 1313 1435 1574 1655 1674 1697 1723 1766
Size: 484 122 139 81 19 23 26 43
Cuts at: 1766 1775 1884
Size: 9 109
Fragments arranged by size:
484 245 159 139 122 111 109 97
84 81 70 43 35 28 26 23
19 9
BbeI G_GCGC'C
Cuts at: 0 104 1100 1884
Size: 104 996 784
Fragments arranged by size:
996 784 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 147 1615 1884
Size: 147 1468 269
Fragments arranged by size:
1468 269 147
BbvCI CC'TCA_GC
Cuts at: 0 1541 1666 1849 1884
Size: 1541 125 183 35
Fragments arranged by size:
1541 183 125 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 861 895 1774 1808 1884
Size: 861 34 879 34 76
Fragments arranged by size:
879 861 76 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 971 1210 1884
Size: 971 239 674
Fragments arranged by size:
971 674 239
BlpI GC'TnA_GC
Cuts at: 0 673 1884
Size: 673 1211
Bme1580I G_kGCm'C
Cuts at: 0 72 91 97 132 167 443 599 794
Size: 72 19 6 35 35 276 156 195
Cuts at: 794 1016 1574 1674 1884
Size: 222 558 100 210
Fragments arranged by size:
558 276 222 210 195 156 100 72
35 35 19 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 157 1884
Size: 157 1727
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 384 1884
Size: 384 1500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1452 1466 1884
Size: 1452 14 418
Fragments arranged by size:
1452 418 14
Bpu10I CC'TnA_GC
Cuts at: 0 1541 1666 1691 1849 1884
Size: 1541 125 25 158 35
Fragments arranged by size:
1541 158 125 35 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 355 1884
Size: 355 1529
BsaAI yAC'GTr
Cuts at: 0 45 1884
Size: 45 1839
BsaHI Gr'CG_yC
Cuts at: 0 101 152 514 605 696 884 1097 1160
Size: 101 51 362 91 91 188 213 63
Cuts at: 1160 1884
Size: 724
Fragments arranged by size:
724 362 213 188 101 91 91 63
51
BsaWI w'CCGG_w
Cuts at: 0 763 1884
Size: 763 1121
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 976 1006 1884
Size: 976 30 878
Fragments arranged by size:
976 878 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 997 1386 1504 1582 1645 1648 1867 1884
Size: 997 389 118 78 63 3 219 17
Fragments arranged by size:
997 389 219 118 78 63 17 3
BseYI C'CCAG_C
Cuts at: 0 53 125 216 261 927 1381 1552 1674
Size: 53 72 91 45 666 454 171 122
Cuts at: 1674 1744 1796 1871 1884
Size: 70 52 75 13
Fragments arranged by size:
666 454 171 122 91 75 72 70
53 52 45 13
BsiEI CG_ry'CG
Cuts at: 0 644 800 1884
Size: 644 156 1084
Fragments arranged by size:
1084 644 156
BsiHKAI G_wGCw'C
Cuts at: 0 91 1016 1766 1884
Size: 91 925 750 118
Fragments arranged by size:
925 750 118 91
BsmI GAATG_Cn'
Cuts at: 0 1858 1884
Size: 1858 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1571 1884
Size: 1571 313
Bsp1286I G_dGCh'C
Cuts at: 0 72 91 97 132 167 326 443 571
Size: 72 19 6 35 35 159 117 128
Cuts at: 571 599 683 794 829 1016 1313 1435 1574
Size: 28 84 111 35 187 297 122 139
Cuts at: 1574 1655 1674 1697 1723 1766 1775 1884
Size: 81 19 23 26 43 9 109
Fragments arranged by size:
297 187 159 139 128 122 117 111
109 84 81 72 43 35 35 35
28 26 23 19 19 9 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1253 1884
Size: 1253 631
BsrBI CCG'CTC
Cuts at: 0 688 1884
Size: 688 1196
BsrFI r'CCGG_y
Cuts at: 0 643 935 1004 1884
Size: 643 292 69 880
Fragments arranged by size:
880 643 292 69
BssHII G'CGCG_C
Cuts at: 0 858 1884
Size: 858 1026
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1254 1884
Size: 1254 630
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1818 1884
Size: 1818 66
BstYI r'GATC_y
Cuts at: 0 1366 1884
Size: 1366 518
Bsu36I CC'TnA_GG
Cuts at: 0 1487 1714 1884
Size: 1487 227 170
Fragments arranged by size:
1487 227 170
BtgI C'CryG_G
Cuts at: 0 249 414 1205 1832 1884
Size: 249 165 791 627 52
Fragments arranged by size:
791 627 249 165 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1683 1884
Size: 1683 201
BtsI GCAGTG_nn'
Cuts at: 0 1181 1194 1435 1884
Size: 1181 13 241 449
Fragments arranged by size:
1181 449 241 13
DrdI GACnn_nn'nnGTC
Cuts at: 0 1087 1884
Size: 1087 797
EaeI y'GGCC_r
Cuts at: 0 495 590 641 797 830 1223 1884
Size: 495 95 51 156 33 393 661
Fragments arranged by size:
661 495 393 156 95 51 33
EagI C'GGCC_G
Cuts at: 0 641 797 1884
Size: 641 156 1087
Fragments arranged by size:
1087 641 156
EarI CTCTTCn'nnn_
Cuts at: 0 1643 1754 1884
Size: 1643 111 130
Fragments arranged by size:
1643 130 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 185 662 707 1884
Size: 185 477 45 1177
Fragments arranged by size:
1177 477 185 45
EcoICRI GAG'CTC
Cuts at: 0 1764 1884
Size: 1764 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1452 1466 1884
Size: 292 1160 14 418
Fragments arranged by size:
1160 418 292 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 820 1168 1318 1445 1884
Size: 820 348 150 127 439
Fragments arranged by size:
820 439 348 150 127
EcoO109I rG'GnC_Cy
Cuts at: 0 94 163 791 1242 1263 1459 1570 1670
Size: 94 69 628 451 21 196 111 100
Cuts at: 1670 1671 1735 1884
Size: 1 64 149
Fragments arranged by size:
628 451 196 149 111 100 94 69
64 21 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 39 1884
Size: 39 1845
FspI TGC'GCA
Cuts at: 0 733 1884
Size: 733 1151
HaeII r_GCGC'y
Cuts at: 0 104 414 460 808 897 1100 1131 1884
Size: 104 310 46 348 89 203 31 753
Fragments arranged by size:
753 348 310 203 104 89 46 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 601 633 1884
Size: 601 32 1251
Fragments arranged by size:
1251 601 32
HindIII A'AGCT_T
Cuts at: 0 1478 1884
Size: 1478 406
KasI G'GCGC_C
Cuts at: 0 100 1096 1884
Size: 100 996 788
Fragments arranged by size:
996 788 100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 923 1325 1884
Size: 395 528 402 559
Fragments arranged by size:
559 528 402 395
MscI TGG'CCA
Cuts at: 0 1225 1884
Size: 1225 659
MslI CAynn'nnrTG
Cuts at: 0 838 1702 1884
Size: 838 864 182
Fragments arranged by size:
864 838 182
MspA1I CmG'CkG
Cuts at: 0 119 239 251 284 677 788 875 1144
Size: 119 120 12 33 393 111 87 269
Cuts at: 1144 1665 1777 1884
Size: 521 112 107
Fragments arranged by size:
521 393 269 120 119 112 111 107
87 33 12
NaeI GCC'GGC
Cuts at: 0 645 1884
Size: 645 1239
NarI GG'CG_CC
Cuts at: 0 101 1097 1884
Size: 101 996 787
Fragments arranged by size:
996 787 101
NgoMIV G'CCGG_C
Cuts at: 0 643 1884
Size: 643 1241
PasI CC'CwG_GG
Cuts at: 0 105 708 1330 1884
Size: 105 603 622 554
Fragments arranged by size:
622 603 554 105
PflMI CCAn_nnn'nTGG
Cuts at: 0 1481 1884
Size: 1481 403
PfoI T'CCnGG_A
Cuts at: 0 341 1443 1841 1884
Size: 341 1102 398 43
Fragments arranged by size:
1102 398 341 43
PpuMI rG'GwC_Cy
Cuts at: 0 1242 1459 1735 1884
Size: 1242 217 276 149
Fragments arranged by size:
1242 276 217 149
PshAI GACnn'nnGTC
Cuts at: 0 1457 1884
Size: 1457 427
PspOMI G'GGCC_C
Cuts at: 0 93 163 595 790 1570 1670 1884
Size: 93 70 432 195 780 100 214
Fragments arranged by size:
780 432 214 195 100 93 70
PspXI vC'TCGA_Gb
Cuts at: 0 1728 1884
Size: 1728 156
PstI C_TGCA'G
Cuts at: 0 1250 1606 1884
Size: 1250 356 278
Fragments arranged by size:
1250 356 278
RsrII CG'GwC_CG
Cuts at: 0 766 1884
Size: 766 1118
SacI G_AGCT'C
Cuts at: 0 1766 1884
Size: 1766 118
SacII CC_GC'GG
Cuts at: 0 252 1884
Size: 252 1632
SanDI GG'GwC_CC
Cuts at: 0 1459 1884
Size: 1459 425
SapI GCTCTTCn'nnn_
Cuts at: 0 1643 1754 1884
Size: 1643 111 130
Fragments arranged by size:
1643 130 111
SfcI C'TryA_G
Cuts at: 0 1246 1602 1884
Size: 1246 356 282
Fragments arranged by size:
1246 356 282
SfoI GGC'GCC
Cuts at: 0 102 1098 1884
Size: 102 996 786
Fragments arranged by size:
996 786 102
SmaI CCC'GGG
Cuts at: 0 9 574 600 1884
Size: 9 565 26 1284
Fragments arranged by size:
1284 565 26 9
SmlI C'TyrA_G
Cuts at: 0 1728 1884
Size: 1728 156
StuI AGG'CCT
Cuts at: 0 1791 1884
Size: 1791 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1736 1884
Size: 1736 148
TatI w'GTAC_w
Cuts at: 0 113 992 1884
Size: 113 879 892
Fragments arranged by size:
892 879 113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1704 1818 1884
Size: 1704 114 66
Fragments arranged by size:
1704 114 66
XhoI C'TCGA_G
Cuts at: 0 1728 1884
Size: 1728 156
XmaI C'CCGG_G
Cuts at: 0 7 572 598 1884
Size: 7 565 26 1286
Fragments arranged by size:
1286 565 26 7
ZraI GAC'GTC
Cuts at: 0 697 1884
Size: 697 1187
Enzymes that do cut:
AatII AcuI AfeI AhdI AleI AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV PasI PflMI PfoI
PpuMI PshAI PspOMI PspXI PstI RsrII SacI SacII
SanDI SapI SfcI SfoI SmaI SmlI StuI TaqII
TatI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AloI
ApoI AscI AseI AsiSI AvrII BaeI BciVI BclI
BfrBI BglII BmgBI BmtI BpuEI BsaBI BsgI BsiWI
BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I
ClaI CspCI DraI DraIII EcoRI EcoRV FseI FspAI
HincII HpaI KpnI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PsiI PsrI PvuI PvuII SalI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StyI SwaI TstI Tth111I XbaI XmnI