Restriction map for
pF1KSDA1835
(Linear) (Six-base) MAPSORT of: KIAA1835.seq Check: 6111 from: 1 to: 1761
KIAA1835.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 783 1761
Size: 783 978
AatII G_ACGT'C
Cuts at: 0 1301 1761
Size: 1301 460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1006 1761
Size: 565 441 755
Fragments arranged by size:
755 565 441
AfeI AGC'GCT
Cuts at: 0 241 1761
Size: 241 1520
AflII C'TTAA_G
Cuts at: 0 354 1761
Size: 354 1407
AflIII A'CryG_T
Cuts at: 0 257 1385 1761
Size: 257 1128 376
Fragments arranged by size:
1128 376 257
AhdI GACnn_n'nnGTC
Cuts at: 0 503 1761
Size: 503 1258
AleI CACnn'nnGTG
Cuts at: 0 251 1761
Size: 251 1510
AlwNI CAG_nnn'CTG
Cuts at: 0 1749 1761
Size: 1749 12
ApaI G_GGCC'C
Cuts at: 0 377 1352 1761
Size: 377 975 409
Fragments arranged by size:
975 409 377
ApaLI G'TGCA_C
Cuts at: 0 1552 1761
Size: 1552 209
AseI AT'TA_AT
Cuts at: 0 806 1761
Size: 806 955
AvaI C'yCGr_G
Cuts at: 0 1396 1761
Size: 1396 365
AvrII C'CTAG_G
Cuts at: 0 1344 1761
Size: 1344 417
BanI G'GyrC_C
Cuts at: 0 586 754 1761
Size: 586 168 1007
Fragments arranged by size:
1007 586 168
BanII G_rGCy'C
Cuts at: 0 336 377 415 1352 1360 1761
Size: 336 41 38 937 8 401
Fragments arranged by size:
937 401 336 41 38 8
BbeI G_GCGC'C
Cuts at: 0 590 758 1761
Size: 590 168 1003
Fragments arranged by size:
1003 590 168
BbsI GAAGACnn'nnnn_
Cuts at: 0 17 143 787 1583 1761
Size: 17 126 644 796 178
Fragments arranged by size:
796 644 178 126 17
BbvCI CC'TCA_GC
Cuts at: 0 941 1193 1761
Size: 941 252 568
Fragments arranged by size:
941 568 252
BglI GCCn_nnn'nGGC
Cuts at: 0 25 1761
Size: 25 1736
BlpI GC'TnA_GC
Cuts at: 0 335 414 962 1761
Size: 335 79 548 799
Fragments arranged by size:
799 548 335 79
Bme1580I G_kGCm'C
Cuts at: 0 377 525 1352 1556 1761
Size: 377 148 827 204 205
Fragments arranged by size:
827 377 205 204 148
BmgBI CAC'GTC
Cuts at: 0 1388 1761
Size: 1388 373
BmrI ACTGGGnnnn_n'
Cuts at: 0 1265 1761
Size: 1265 496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 595 649 988 1054 1310 1761
Size: 270 325 54 339 66 256 451
Fragments arranged by size:
451 339 325 270 256 66 54
Bpu10I CC'TnA_GC
Cuts at: 0 941 1193 1761
Size: 941 252 568
Fragments arranged by size:
941 568 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 424 1552 1761
Size: 391 33 1128 209
Fragments arranged by size:
1128 391 209 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 766 1413 1761
Size: 766 647 348
Fragments arranged by size:
766 647 348
BsaHI Gr'CG_yC
Cuts at: 0 587 755 1063 1298 1761
Size: 587 168 308 235 463
Fragments arranged by size:
587 463 308 235 168
BsaWI w'CCGG_w
Cuts at: 0 509 1761
Size: 509 1252
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 646 1102 1105 1108 1111 1150 1153 1171
Size: 646 456 3 3 3 39 3 18
Cuts at: 1171 1183 1186 1189 1192 1500 1537 1761
Size: 12 3 3 3 308 37 224
Fragments arranged by size:
646 456 308 224 39 37 18 12
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 328 1373 1761
Size: 328 1045 388
Fragments arranged by size:
1045 388 328
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 860 1489 1657 1761
Size: 860 629 168 104
Fragments arranged by size:
860 629 168 104
BsiHKAI G_wGCw'C
Cuts at: 0 415 1360 1556 1761
Size: 415 945 196 205
Fragments arranged by size:
945 415 205 196
BsmI GAATG_Cn'
Cuts at: 0 367 1761
Size: 367 1394
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1305 1761
Size: 1305 456
Bsp1286I G_dGCh'C
Cuts at: 0 336 377 415 525 1352 1360 1556 1761
Size: 336 41 38 110 827 8 196 205
Fragments arranged by size:
827 336 205 196 110 41 38 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 783 1761
Size: 783 978
BsrDI GCAATG_nn'
Cuts at: 0 14 848 1592 1761
Size: 14 834 744 169
Fragments arranged by size:
834 744 169 14
BsrGI T'GTAC_A
Cuts at: 0 1751 1761
Size: 1751 10
BssSI C'ACGA_G
Cuts at: 0 1549 1761
Size: 1549 212
BstAPI GCAn_nnn'nTGC
Cuts at: 0 109 1483 1761
Size: 109 1374 278
Fragments arranged by size:
1374 278 109
BstXI CCAn_nnnn'nTGG
Cuts at: 0 597 1059 1311 1761
Size: 597 462 252 450
Fragments arranged by size:
597 462 450 252
BstYI r'GATC_y
Cuts at: 0 284 479 1761
Size: 284 195 1282
Fragments arranged by size:
1282 284 195
BtgI C'CryG_G
Cuts at: 0 758 764 864 947 1761
Size: 758 6 100 83 814
Fragments arranged by size:
814 758 100 83 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 189 1761
Size: 189 1572
BtsI GCAGTG_nn'
Cuts at: 0 98 590 674 1472 1761
Size: 98 492 84 798 289
Fragments arranged by size:
798 492 289 98 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 435 470 1761
Size: 435 35 1291
Fragments arranged by size:
1291 435 35
DraIII CAC_nnn'GTG
Cuts at: 0 1635 1761
Size: 1635 126
DrdI GACnn_nn'nnGTC
Cuts at: 0 1386 1761
Size: 1386 375
EaeI y'GGCC_r
Cuts at: 0 53 761 767 1049 1761
Size: 53 708 6 282 712
Fragments arranged by size:
712 708 282 53 6
EarI CTCTTCn'nnn_
Cuts at: 0 1103 1112 1127 1148 1160 1181 1761
Size: 1103 9 15 21 12 21 580
Fragments arranged by size:
1103 580 21 21 15 12 9
EcoICRI GAG'CTC
Cuts at: 0 413 1358 1761
Size: 413 945 403
Fragments arranged by size:
945 413 403
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 565 595 649 988 1006 1054 1310
Size: 270 295 30 54 339 18 48 256
Cuts at: 1310 1761
Size: 451
Fragments arranged by size:
451 339 295 270 256 54 48 30
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 631 685 1761
Size: 631 54 1076
Fragments arranged by size:
1076 631 54
EcoO109I rG'GnC_Cy
Cuts at: 0 399 623 1348 1659 1761
Size: 399 224 725 311 102
Fragments arranged by size:
725 399 311 224 102
HaeII r_GCGC'y
Cuts at: 0 243 590 758 1624 1706 1761
Size: 243 347 168 866 82 55
Fragments arranged by size:
866 347 243 168 82 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 211 243 489 521 1761
Size: 211 32 246 32 1240
Fragments arranged by size:
1240 246 211 32 32
HincII GTy'rAC
Cuts at: 0 702 1761
Size: 702 1059
HindIII A'AGCT_T
Cuts at: 0 605 1761
Size: 605 1156
KasI G'GCGC_C
Cuts at: 0 586 754 1761
Size: 586 168 1007
Fragments arranged by size:
1007 586 168
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 1550 1761
Size: 209 1341 211
Fragments arranged by size:
1341 211 209
MscI TGG'CCA
Cuts at: 0 763 1051 1761
Size: 763 288 710
Fragments arranged by size:
763 710 288
MslI CAynn'nnrTG
Cuts at: 0 251 1761
Size: 251 1510
MspA1I CmG'CkG
Cuts at: 0 66 328 339 866 1761
Size: 66 262 11 527 895
Fragments arranged by size:
895 527 262 66 11
NarI GG'CG_CC
Cuts at: 0 587 755 1761
Size: 587 168 1006
Fragments arranged by size:
1006 587 168
NcoI C'CATG_G
Cuts at: 0 764 947 1761
Size: 764 183 814
Fragments arranged by size:
814 764 183
NspI r_CATG'y
Cuts at: 0 261 645 1761
Size: 261 384 1116
Fragments arranged by size:
1116 384 261
PasI CC'CwG_GG
Cuts at: 0 996 1761
Size: 996 765
PciI A'CATG_T
Cuts at: 0 257 1761
Size: 257 1504
PflMI CCAn_nnn'nTGG
Cuts at: 0 652 1761
Size: 652 1109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 428 460 1761
Size: 428 32 1301
Fragments arranged by size:
1301 428 32
PpuMI rG'GwC_Cy
Cuts at: 0 623 1761
Size: 623 1138
PspOMI G'GGCC_C
Cuts at: 0 373 1348 1761
Size: 373 975 413
Fragments arranged by size:
975 413 373
PstI C_TGCA'G
Cuts at: 0 105 434 492 1296 1745 1761
Size: 105 329 58 804 449 16
Fragments arranged by size:
804 449 329 105 58 16
PvuII CAG'CTG
Cuts at: 0 66 328 339 1761
Size: 66 262 11 1422
Fragments arranged by size:
1422 262 66 11
RsrII CG'GwC_CG
Cuts at: 0 278 1761
Size: 278 1483
SacI G_AGCT'C
Cuts at: 0 415 1360 1761
Size: 415 945 401
Fragments arranged by size:
945 415 401
SacII CC_GC'GG
Cuts at: 0 867 1761
Size: 867 894
SanDI GG'GwC_CC
Cuts at: 0 623 1761
Size: 623 1138
SapI GCTCTTCn'nnn_
Cuts at: 0 1181 1761
Size: 1181 580
SbfI CC_TGCA'GG
Cuts at: 0 434 1761
Size: 434 1327
SfcI C'TryA_G
Cuts at: 0 101 430 488 1292 1741 1761
Size: 101 329 58 804 449 20
Fragments arranged by size:
804 449 329 101 58 20
SfoI GGC'GCC
Cuts at: 0 588 756 1761
Size: 588 168 1005
Fragments arranged by size:
1005 588 168
SmlI C'TyrA_G
Cuts at: 0 354 406 439 1567 1761
Size: 354 52 33 1128 194
Fragments arranged by size:
1128 354 194 52 33
StuI AGG'CCT
Cuts at: 0 214 1761
Size: 214 1547
StyI C'CwwG_G
Cuts at: 0 209 525 596 764 833 947 1052 1344
Size: 209 316 71 168 69 114 105 292
Cuts at: 1344 1655 1761
Size: 311 106
Fragments arranged by size:
316 311 292 209 168 114 106 105
71 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 295 514 1761
Size: 295 219 1247
Fragments arranged by size:
1247 295 219
TatI w'GTAC_w
Cuts at: 0 1751 1761
Size: 1751 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 499 504 531 536 1761
Size: 499 5 27 5 1225
Fragments arranged by size:
1225 499 27 5 5
Tth111I GACn'n_nGTC
Cuts at: 0 1582 1761
Size: 1582 179
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 48 1704 1761
Size: 48 1656 57
Fragments arranged by size:
1656 57 48
XmnI GAAnn'nnTTC
Cuts at: 0 1019 1501 1761
Size: 1019 482 260
Fragments arranged by size:
1019 482 260
ZraI GAC'GTC
Cuts at: 0 1299 1761
Size: 1299 462
Enzymes that do cut:
AarI AatII AcuI AfeI AflII AflIII AhdI AleI
AlwNI ApaI ApaLI AseI AvaI AvrII BanI BanII
BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI
BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NarI NcoI NspI PasI PciI PflMI PpiI
PpuMI PspOMI PstI PvuII RsrII SacI SacII SanDI
SapI SbfI SfcI SfoI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AgeI AlfI AloI ApoI AscI
AsiSI BaeI BamHI BcgI BciVI BclI BfrBI BglII
BmtI BplI BsaAI BsaBI BsaXI BsiEI BsiWI BspEI
BspHI BsrBI BsrFI BssHII BstBI BstEII BstZ17I Bsu36I
ClaI DraI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HpaI KpnI MfeI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI SalI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmaI