Restriction map for
pF1KA1839
(Linear) (Six-base) MAPSORT of: KIAA1839.seq Check: 6296 from: 1 to: 1551
KIAA1839.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 107 1551
Size: 107 1444
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 320 781 902 1079 1139 1551
Size: 160 160 461 121 177 60 412
Fragments arranged by size:
461 412 177 160 160 121 60
AflIII A'CryG_T
Cuts at: 0 1287 1551
Size: 1287 264
AleI CACnn'nnGTG
Cuts at: 0 876 1551
Size: 876 675
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 38 1551
Size: 38 1513
AlwNI CAG_nnn'CTG
Cuts at: 0 343 1551
Size: 343 1208
ApoI r'AATT_y
Cuts at: 0 292 459 579 964 1193 1262 1275 1551
Size: 292 167 120 385 229 69 13 276
Fragments arranged by size:
385 292 276 229 167 120 69 13
AseI AT'TA_AT
Cuts at: 0 778 1551
Size: 778 773
AvaI C'yCGr_G
Cuts at: 0 10 68 1551
Size: 10 58 1483
Fragments arranged by size:
1483 58 10
BanI G'GyrC_C
Cuts at: 0 95 1551
Size: 95 1456
BanII G_rGCy'C
Cuts at: 0 49 156 1551
Size: 49 107 1395
Fragments arranged by size:
1395 107 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 635 1142 1551
Size: 635 507 409
Fragments arranged by size:
635 507 409
BbvCI CC'TCA_GC
Cuts at: 0 119 671 1551
Size: 119 552 880
Fragments arranged by size:
880 552 119
BfrBI ATG'CAT
Cuts at: 0 1069 1551
Size: 1069 482
BlpI GC'TnA_GC
Cuts at: 0 107 1551
Size: 107 1444
Bpu10I CC'TnA_GC
Cuts at: 0 119 671 1551
Size: 119 552 880
Fragments arranged by size:
880 552 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1551
Size: 454 1097
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 42 136 659 1138 1551
Size: 39 3 94 523 479 413
Fragments arranged by size:
523 479 413 94 39 3
BsiEI CG_ry'CG
Cuts at: 0 78 1551
Size: 78 1473
BsiHKAI G_wGCw'C
Cuts at: 0 49 1551
Size: 49 1502
Bsp1286I G_dGCh'C
Cuts at: 0 49 156 1551
Size: 49 107 1395
Fragments arranged by size:
1395 107 49
BspHI T'CATG_A
Cuts at: 0 1041 1551
Size: 1041 510
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 1059 1551
Size: 107 952 492
Fragments arranged by size:
952 492 107
BsrDI GCAATG_nn'
Cuts at: 0 640 1551
Size: 640 911
BsrFI r'CCGG_y
Cuts at: 0 102 1551
Size: 102 1449
BsrGI T'GTAC_A
Cuts at: 0 549 1551
Size: 549 1002
BssSI C'ACGA_G
Cuts at: 0 42 1551
Size: 42 1509
BstYI r'GATC_y
Cuts at: 0 1524 1551
Size: 1524 27
Bsu36I CC'TnA_GG
Cuts at: 0 680 1529 1551
Size: 680 849 22
Fragments arranged by size:
849 680 22
BtgI C'CryG_G
Cuts at: 0 438 1484 1551
Size: 438 1046 67
Fragments arranged by size:
1046 438 67
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 576 611 1551
Size: 576 35 940
Fragments arranged by size:
940 576 35
DraI TTT'AAA
Cuts at: 0 458 1551
Size: 458 1093
EarI CTCTTCn'nnn_
Cuts at: 0 659 1551
Size: 659 892
EcoICRI GAG'CTC
Cuts at: 0 47 1551
Size: 47 1504
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 320 781 902 1079 1139 1551
Size: 160 160 461 121 177 60 412
Fragments arranged by size:
461 412 177 160 160 121 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1532 1551
Size: 1532 19
EcoO109I rG'GnC_Cy
Cuts at: 0 168 555 1302 1551
Size: 168 387 747 249
Fragments arranged by size:
747 387 249 168
EcoRI G'AATT_C
Cuts at: 0 964 1551
Size: 964 587
EcoRV GAT'ATC
Cuts at: 0 29 1551
Size: 29 1522
HaeII r_GCGC'y
Cuts at: 0 831 1551
Size: 831 720
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 700 732 1324 1356 1551
Size: 700 32 592 32 195
Fragments arranged by size:
700 592 195 32 32
HincII GTy'rAC
Cuts at: 0 1332 1551
Size: 1332 219
HindIII A'AGCT_T
Cuts at: 0 1403 1551
Size: 1403 148
MslI CAynn'nnrTG
Cuts at: 0 876 1551
Size: 876 675
MspA1I CmG'CkG
Cuts at: 0 991 1353 1551
Size: 991 362 198
Fragments arranged by size:
991 362 198
NaeI GCC'GGC
Cuts at: 0 104 1551
Size: 104 1447
NcoI C'CATG_G
Cuts at: 0 438 1484 1551
Size: 438 1046 67
Fragments arranged by size:
1046 438 67
NgoMIV G'CCGG_C
Cuts at: 0 102 1551
Size: 102 1449
NsiI A_TGCA'T
Cuts at: 0 1071 1551
Size: 1071 480
NspI r_CATG'y
Cuts at: 0 649 987 1291 1551
Size: 649 338 304 260
Fragments arranged by size:
649 338 304 260
PciI A'CATG_T
Cuts at: 0 1287 1551
Size: 1287 264
PflMI CCAn_nnn'nTGG
Cuts at: 0 439 1551
Size: 439 1112
PpuMI rG'GwC_Cy
Cuts at: 0 168 555 1302 1551
Size: 168 387 747 249
Fragments arranged by size:
747 387 249 168
PvuII CAG'CTG
Cuts at: 0 991 1551
Size: 991 560
SacI G_AGCT'C
Cuts at: 0 49 1551
Size: 49 1502
ScaI AGT'ACT
Cuts at: 0 145 1551
Size: 145 1406
SfcI C'TryA_G
Cuts at: 0 944 1257 1551
Size: 944 313 294
Fragments arranged by size:
944 313 294
SmlI C'TyrA_G
Cuts at: 0 469 1551
Size: 469 1082
SphI G_CATG'C
Cuts at: 0 649 1551
Size: 649 902
StyI C'CwwG_G
Cuts at: 0 438 524 1484 1551
Size: 438 86 960 67
Fragments arranged by size:
960 438 86 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 92 675 773 1551
Size: 92 583 98 778
Fragments arranged by size:
778 583 98 92
TatI w'GTAC_w
Cuts at: 0 143 549 1551
Size: 143 406 1002
Fragments arranged by size:
1002 406 143
Tth111I GACn'n_nGTC
Cuts at: 0 370 1551
Size: 370 1181
XbaI T'CTAG_A
Cuts at: 0 1168 1198 1551
Size: 1168 30 353
Fragments arranged by size:
1168 353 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1107 1551
Size: 1107 444
XmnI GAAnn'nnTTC
Cuts at: 0 145 1551
Size: 145 1406
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApoI AseI
AvaI BanI BanII BbsI BbvCI BfrBI BlpI Bpu10I
BpuEI BseRI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstYI Bsu36I BtgI CspCI DraI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV HaeII
Hin4I HincII HindIII MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PciI PflMI PpuMI PvuII SacI ScaI
SfcI SmlI SphI StyI TaqII TatI Tth111I XbaI
XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BglI BglII Bme1580I BmgBI
BmrI BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiWI BsmI BsmBI BspEI
BsrBI BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI
BtsI ClaI DraIII DrdI EaeI EagI EciI FalI
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NdeI NheI NotI NruI PacI
PasI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TstI XhoI
XmaI ZraI