Restriction map for
pF1KA1840
(Linear) (Six-base) MAPSORT of: KIAA1840.seq Check: 9707 from: 1 to: 7329
KIAA1840.orf
With 182 enzymes: SgfI *
July 14, 2018 20:54 ..
AccI GT'mk_AC
Cuts at: 0 1415 3020 3120 5973 7329
Size: 1415 1605 100 2853 1356
Fragments arranged by size:
2853 1605 1415 1356 100
Acc65I G'GTAC_C
Cuts at: 0 6589 7329
Size: 6589 740
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1907 2106 2660 2719 4167 4520 4668 4724
Size: 1907 199 554 59 1448 353 148 56
Cuts at: 4724 4990 6235 6657 7213 7303 7329
Size: 266 1245 422 556 90 26
Fragments arranged by size:
1907 1448 1245 556 554 422 353 266
199 148 90 59 56 26
AflII C'TTAA_G
Cuts at: 0 4206 6130 6796 7329
Size: 4206 1924 666 533
Fragments arranged by size:
4206 1924 666 533
AflIII A'CryG_T
Cuts at: 0 731 7329
Size: 731 6598
AleI CACnn'nnGTG
Cuts at: 0 1253 4477 7329
Size: 1253 3224 2852
Fragments arranged by size:
3224 2852 1253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2741 5363 7329
Size: 2741 2622 1966
Fragments arranged by size:
2741 2622 1966
AlwNI CAG_nnn'CTG
Cuts at: 0 1437 2447 6142 7329
Size: 1437 1010 3695 1187
Fragments arranged by size:
3695 1437 1187 1010
ApaI G_GGCC'C
Cuts at: 0 247 7329
Size: 247 7082
ApaLI G'TGCA_C
Cuts at: 0 3399 7329
Size: 3399 3930
ApoI r'AATT_y
Cuts at: 0 349 632 855 1635 2178 2321 2521 2704
Size: 349 283 223 780 543 143 200 183
Cuts at: 2704 2813 3103 3591 4328 5208 5220 7046 7096
Size: 109 290 488 737 880 12 1826 50
Cuts at: 7096 7329
Size: 233
Fragments arranged by size:
1826 880 780 737 543 488 349 290
283 233 223 200 183 143 109 50
12
AseI AT'TA_AT
Cuts at: 0 1940 3658 4967 7329
Size: 1940 1718 1309 2362
Fragments arranged by size:
2362 1940 1718 1309
AvaI C'yCGr_G
Cuts at: 0 128 7329
Size: 128 7201
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 680 713 7329
Size: 680 33 6616
Fragments arranged by size:
6616 680 33
BamHI G'GATC_C
Cuts at: 0 3333 6579 7329
Size: 3333 3246 750
Fragments arranged by size:
3333 3246 750
BanI G'GyrC_C
Cuts at: 0 52 90 4435 5364 5648 6589 6773 7329
Size: 52 38 4345 929 284 941 184 556
Fragments arranged by size:
4345 941 929 556 284 184 52 38
BanII G_rGCy'C
Cuts at: 0 247 2562 3725 5459 6105 6353 7329
Size: 247 2315 1163 1734 646 248 976
Fragments arranged by size:
2315 1734 1163 976 646 248 247
BbeI G_GCGC'C
Cuts at: 0 5652 7329
Size: 5652 1677
BbsI GAAGACnn'nnnn_
Cuts at: 0 734 2870 7329
Size: 734 2136 4459
Fragments arranged by size:
4459 2136 734
BbvCI CC'TCA_GC
Cuts at: 0 7013 7329
Size: 7013 316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6694 6728 7329
Size: 6694 34 601
Fragments arranged by size:
6694 601 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2910 4944 7329
Size: 2910 2034 2385
Fragments arranged by size:
2910 2385 2034
BclI T'GATC_A
Cuts at: 0 1695 1704 7329
Size: 1695 9 5625
Fragments arranged by size:
5625 1695 9
BfrBI ATG'CAT
Cuts at: 0 1176 3523 7329
Size: 1176 2347 3806
Fragments arranged by size:
3806 2347 1176
BglI GCCn_nnn'nGGC
Cuts at: 0 242 5272 7329
Size: 242 5030 2057
Fragments arranged by size:
5030 2057 242
BglII A'GATC_T
Cuts at: 0 909 2273 5020 5399 5474 7329
Size: 909 1364 2747 379 75 1855
Fragments arranged by size:
2747 1855 1364 909 379 75
BlpI GC'TnA_GC
Cuts at: 0 5813 7329
Size: 5813 1516
Bme1580I G_kGCm'C
Cuts at: 0 55 247 3403 4241 4282 5342 5369 5903
Size: 55 192 3156 838 41 1060 27 534
Cuts at: 5903 6776 7329
Size: 873 553
Fragments arranged by size:
3156 1060 873 838 553 534 192 55
41 27
BmgBI CAC'GTC
Cuts at: 0 5361 6386 7329
Size: 5361 1025 943
Fragments arranged by size:
5361 1025 943
BmrI ACTGGGnnnn_n'
Cuts at: 0 4183 4839 6449 7329
Size: 4183 656 1610 880
Fragments arranged by size:
4183 1610 880 656
BmtI G_CTAG'C
Cuts at: 0 3170 7324 7329
Size: 3170 4154 5
Fragments arranged by size:
4154 3170 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3689 4374 4619 4909 7329
Size: 3689 685 245 290 2420
Fragments arranged by size:
3689 2420 685 290 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1081 2670 4414 4590 4738 4813 5401
Size: 259 822 1589 1744 176 148 75 588
Cuts at: 5401 5410 5889 6656 7329
Size: 9 479 767 673
Fragments arranged by size:
1744 1589 822 767 673 588 479 259
176 148 75 9
Bpu10I CC'TnA_GC
Cuts at: 0 6038 7013 7329
Size: 6038 975 316
Fragments arranged by size:
6038 975 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 1475 2173 2254 3781 4570 5842 6748
Size: 779 696 698 81 1527 789 1272 906
Cuts at: 6748 7329
Size: 581
Fragments arranged by size:
1527 1272 906 789 779 698 696 581
81
BsaI GGTCTCn'nnnn_
Cuts at: 0 1360 1421 7329
Size: 1360 61 5908
Fragments arranged by size:
5908 1360 61
BsaAI yAC'GTr
Cuts at: 0 2305 7329
Size: 2305 5024
BsaBI GATnn'nnATC
Cuts at: 0 1901 7329
Size: 1901 5428
BsaHI Gr'CG_yC
Cuts at: 0 205 5649 7329
Size: 205 5444 1680
Fragments arranged by size:
5444 1680 205
BsaWI w'CCGG_w
Cuts at: 0 6059 7329
Size: 6059 1270
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3227 3257 7329
Size: 3227 30 4072
Fragments arranged by size:
4072 3227 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 816 2170 3395 3717 4363 5323 5398 6475
Size: 816 1354 1225 322 646 960 75 1077
Cuts at: 6475 7329
Size: 854
Fragments arranged by size:
1354 1225 1077 960 854 816 646 322
75
BseYI C'CCAG_C
Cuts at: 0 47 1019 1148 1565 2714 3338 4152 4854
Size: 47 972 129 417 1149 624 814 702
Cuts at: 4854 5420 6863 7329
Size: 566 1443 466
Fragments arranged by size:
1443 1149 972 814 702 624 566 466
417 129 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1162 1292 3867 4060 5764 7329
Size: 572 590 130 2575 193 1704 1565
Fragments arranged by size:
2575 1704 1565 590 572 193 130
BsiHKAI G_wGCw'C
Cuts at: 0 1080 1579 2996 3403 5285 5748 6353 7329
Size: 1080 499 1417 407 1882 463 605 976
Fragments arranged by size:
1882 1417 1080 976 605 499 463 407
BsmI GAATG_Cn'
Cuts at: 0 2878 3977 7329
Size: 2878 1099 3352
Fragments arranged by size:
3352 2878 1099
Bsp1286I G_dGCh'C
Cuts at: 0 55 247 1080 1579 2562 2996 3403 3725
Size: 55 192 833 499 983 434 407 322
Cuts at: 3725 4241 4282 5285 5342 5369 5459 5748 5903
Size: 516 41 1003 57 27 90 289 155
Cuts at: 5903 6105 6353 6776 7329
Size: 202 248 423 553
Fragments arranged by size:
1003 983 833 553 516 499 434 423
407 322 289 248 202 192 155 90
57 55 41 27
BspEI T'CCGG_A
Cuts at: 0 6059 7329
Size: 6059 1270
BspHI T'CATG_A
Cuts at: 0 1514 1958 7329
Size: 1514 444 5371
Fragments arranged by size:
5371 1514 444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 7315 7329
Size: 7315 14
BsrDI GCAATG_nn'
Cuts at: 0 409 3708 4511 6369 7329
Size: 409 3299 803 1858 960
Fragments arranged by size:
3299 1858 960 803 409
BsrFI r'CCGG_y
Cuts at: 0 37 5693 7329
Size: 37 5656 1636
Fragments arranged by size:
5656 1636 37
BsrGI T'GTAC_A
Cuts at: 0 2007 7329
Size: 2007 5322
BssSI C'ACGA_G
Cuts at: 0 6154 6703 7329
Size: 6154 549 626
Fragments arranged by size:
6154 626 549
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3000 4277 4934 7329
Size: 3000 1277 657 2395
Fragments arranged by size:
3000 2395 1277 657
BstBI TT'CG_AA
Cuts at: 0 1950 7329
Size: 1950 5379
BstEII G'GTnAC_C
Cuts at: 0 1123 7329
Size: 1123 6206
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1539 4361 4791 5294 6009 7329
Size: 1539 2822 430 503 715 1320
Fragments arranged by size:
2822 1539 1320 715 503 430
BstYI r'GATC_y
Cuts at: 0 662 909 2273 3144 3333 4554 5020 5399
Size: 662 247 1364 871 189 1221 466 379
Cuts at: 5399 5474 5775 5789 6579 7329
Size: 75 301 14 790 750
Fragments arranged by size:
1364 1221 871 790 750 662 466 379
301 247 189 75 14
Bsu36I CC'TnA_GG
Cuts at: 0 929 5754 5848 7329
Size: 929 4825 94 1481
Fragments arranged by size:
4825 1481 929 94
BtgI C'CryG_G
Cuts at: 0 56 62 1081 1521 5955 6324 7329
Size: 56 6 1019 440 4434 369 1005
Fragments arranged by size:
4434 1019 1005 440 369 56 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 124 7329
Size: 124 7205
BtsI GCAGTG_nn'
Cuts at: 0 602 818 1304 1387 4391 4840 5736 7329
Size: 602 216 486 83 3004 449 896 1593
Fragments arranged by size:
3004 1593 896 602 486 449 216 83
DraI TTT'AAA
Cuts at: 0 1505 2090 2198 2327 7329
Size: 1505 585 108 129 5002
Fragments arranged by size:
5002 1505 585 129 108
DraIII CAC_nnn'GTG
Cuts at: 0 1403 6266 7329
Size: 1403 4863 1063
Fragments arranged by size:
4863 1403 1063
EaeI y'GGCC_r
Cuts at: 0 59 980 2828 3619 3821 5063 6921 7329
Size: 59 921 1848 791 202 1242 1858 408
Fragments arranged by size:
1858 1848 1242 921 791 408 202 59
EarI CTCTTCn'nnn_
Cuts at: 0 1490 1642 1736 3394 4723 4900 6158 7130
Size: 1490 152 94 1658 1329 177 1258 972
Cuts at: 7130 7329
Size: 199
Fragments arranged by size:
1658 1490 1329 1258 972 199 177 152
94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 24 7329
Size: 24 7305
EcoICRI GAG'CTC
Cuts at: 0 6351 7329
Size: 6351 978
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1081 1907 2106 2660 2670 2719 4167
Size: 259 822 826 199 554 10 49 1448
Cuts at: 4167 4414 4520 4590 4668 4724 4738 4813 4990
Size: 247 106 70 78 56 14 75 177
Cuts at: 4990 5401 5410 5889 6235 6656 6657 7213 7303
Size: 411 9 479 346 421 1 556 90
Cuts at: 7303 7329
Size: 26
Fragments arranged by size:
1448 826 822 556 554 479 421 411
346 259 247 199 177 106 90 78
75 70 56 49 26 14 10 9
1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 920 3746 7295 7329
Size: 920 2826 3549 34
Fragments arranged by size:
3549 2826 920 34
EcoO109I rG'GnC_Cy
Cuts at: 0 243 244 925 4398 5979 6126 6801 7287
Size: 243 1 681 3473 1581 147 675 486
Cuts at: 7287 7329
Size: 42
Fragments arranged by size:
3473 1581 681 675 486 243 147 42
1
EcoRI G'AATT_C
Cuts at: 0 632 7329
Size: 632 6697
EcoRV GAT'ATC
Cuts at: 0 438 7329
Size: 438 6891
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1008 2295 3961 4094 4232 4729 6567 7084
Size: 1008 1287 1666 133 138 497 1838 517
Cuts at: 7084 7329
Size: 245
Fragments arranged by size:
1838 1666 1287 1008 517 497 245 138
133
FspI TGC'GCA
Cuts at: 0 142 7329
Size: 142 7187
HaeII r_GCGC'y
Cuts at: 0 2084 5316 5652 7329
Size: 2084 3232 336 1677
Fragments arranged by size:
3232 2084 1677 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1206 1238 1502 1534 1599 1631 1696 1728
Size: 1206 32 264 32 65 32 65 32
Cuts at: 1728 2118 2150 3227 3259 4150 4182 5611 5643
Size: 390 32 1077 32 891 32 1429 32
Cuts at: 5643 5781 5813 5966 5998 6726 6758 7329
Size: 138 32 153 32 728 32 571
Fragments arranged by size:
1429 1206 1077 891 728 571 390 264
153 138 65 65 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 957 3121 5974 7329
Size: 957 2164 2853 1355
Fragments arranged by size:
2853 2164 1355 957
HindIII A'AGCT_T
Cuts at: 0 2392 4819 4861 4921 7329
Size: 2392 2427 42 60 2408
Fragments arranged by size:
2427 2408 2392 60 42
HpaI GTT'AAC
Cuts at: 0 957 7329
Size: 957 6372
KasI G'GCGC_C
Cuts at: 0 5648 7329
Size: 5648 1681
KpnI G_GTAC'C
Cuts at: 0 6593 7329
Size: 6593 736
MfeI C'AATT_G
Cuts at: 0 1825 2263 7329
Size: 1825 438 5066
Fragments arranged by size:
5066 1825 438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 6281 6557 6747 6797 7329
Size: 854 5427 276 190 50 532
Fragments arranged by size:
5427 854 532 276 190 50
MscI TGG'CCA
Cuts at: 0 982 2830 3823 5065 6923 7329
Size: 982 1848 993 1242 1858 406
Fragments arranged by size:
1858 1848 1242 993 982 406
MslI CAynn'nnrTG
Cuts at: 0 1253 1526 1559 2015 2723 4477 7180 7329
Size: 1253 273 33 456 708 1754 2703 149
Fragments arranged by size:
2703 1754 1253 708 456 273 149 33
MspA1I CmG'CkG
Cuts at: 0 58 138 1437 1623 3684 6255 6712 6780
Size: 58 80 1299 186 2061 2571 457 68
Cuts at: 6780 6876 7329
Size: 96 453
Fragments arranged by size:
2571 2061 1299 457 453 186 96 80
68 58
NaeI GCC'GGC
Cuts at: 0 39 7329
Size: 39 7290
NarI GG'CG_CC
Cuts at: 0 5649 7329
Size: 5649 1680
NcoI C'CATG_G
Cuts at: 0 62 1081 1521 5955 6324 7329
Size: 62 1019 440 4434 369 1005
Fragments arranged by size:
4434 1019 1005 440 369 62
NdeI CA'TA_TG
Cuts at: 0 693 7329
Size: 693 6636
NgoMIV G'CCGG_C
Cuts at: 0 37 7329
Size: 37 7292
NheI G'CTAG_C
Cuts at: 0 3166 7320 7329
Size: 3166 4154 9
Fragments arranged by size:
4154 3166 9
NruI TCG'CGA
Cuts at: 0 22 7329
Size: 22 7307
NsiI A_TGCA'T
Cuts at: 0 1178 3525 7329
Size: 1178 2347 3804
Fragments arranged by size:
3804 2347 1178
NspI r_CATG'y
Cuts at: 0 735 1380 1594 3761 5279 5597 6432 6681
Size: 735 645 214 2167 1518 318 835 249
Cuts at: 6681 7320 7329
Size: 639 9
Fragments arranged by size:
2167 1518 835 735 645 639 318 249
214 9
PasI CC'CwG_GG
Cuts at: 0 1371 6749 7329
Size: 1371 5378 580
Fragments arranged by size:
5378 1371 580
PciI A'CATG_T
Cuts at: 0 731 7329
Size: 731 6598
PflMI CCAn_nnn'nTGG
Cuts at: 0 2447 7329
Size: 2447 4882
PfoI T'CCnGG_A
Cuts at: 0 1088 1224 3650 6633 7329
Size: 1088 136 2426 2983 696
Fragments arranged by size:
2983 2426 1088 696 136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4216 4248 7329
Size: 4216 32 3081
Fragments arranged by size:
4216 3081 32
PpuMI rG'GwC_Cy
Cuts at: 0 925 5979 7287 7329
Size: 925 5054 1308 42
Fragments arranged by size:
5054 1308 925 42
PsiI TTA'TAA
Cuts at: 0 4694 5711 7329
Size: 4694 1017 1618
Fragments arranged by size:
4694 1618 1017
PspOMI G'GGCC_C
Cuts at: 0 243 7329
Size: 243 7086
PstI C_TGCA'G
Cuts at: 0 9 628 3005 4051 4118 5042 5743 6902
Size: 9 619 2377 1046 67 924 701 1159
Cuts at: 6902 7329
Size: 427
Fragments arranged by size:
2377 1159 1046 924 701 619 427 67
9
PvuII CAG'CTG
Cuts at: 0 138 1437 1623 3684 6255 6712 6780 7329
Size: 138 1299 186 2061 2571 457 68 549
Fragments arranged by size:
2571 2061 1299 549 457 186 138 68
SacI G_AGCT'C
Cuts at: 0 6353 7329
Size: 6353 976
SacII CC_GC'GG
Cuts at: 0 59 7329
Size: 59 7270
SalI G'TCGA_C
Cuts at: 0 3119 5972 7329
Size: 3119 2853 1357
Fragments arranged by size:
3119 2853 1357
SapI GCTCTTCn'nnn_
Cuts at: 0 1642 1736 4723 7329
Size: 1642 94 2987 2606
Fragments arranged by size:
2987 2606 1642 94
ScaI AGT'ACT
Cuts at: 0 560 4180 7329
Size: 560 3620 3149
Fragments arranged by size:
3620 3149 560
SexAI A'CCwGG_T
Cuts at: 0 3557 7329
Size: 3557 3772
SfcI C'TryA_G
Cuts at: 0 5 396 624 1195 1412 1991 2366 3001
Size: 5 391 228 571 217 579 375 635
Cuts at: 3001 3512 3575 3887 4047 4114 4828 4989 5038
Size: 511 63 312 160 67 714 161 49
Cuts at: 5038 5739 6415 6898 7329
Size: 701 676 483 431
Fragments arranged by size:
714 701 676 635 579 571 511 483
431 391 375 312 228 217 161 160
67 63 49 5
SfoI GGC'GCC
Cuts at: 0 5650 7329
Size: 5650 1679
SgrAI Cr'CCGG_yG
Cuts at: 0 37 7329
Size: 37 7292
SmaI CCC'GGG
Cuts at: 0 130 7329
Size: 130 7199
SmlI C'TyrA_G
Cuts at: 0 794 1490 2152 2269 3796 4206 4549 5821
Size: 794 696 662 117 1527 410 343 1272
Cuts at: 5821 6130 6763 6796 7329
Size: 309 633 33 533
Fragments arranged by size:
1527 1272 794 696 662 633 533 410
343 309 117 33
SpeI A'CTAG_T
Cuts at: 0 5482 7329
Size: 5482 1847
SphI G_CATG'C
Cuts at: 0 1380 7329
Size: 1380 5949
StuI AGG'CCT
Cuts at: 0 2174 6109 7018 7329
Size: 2174 3935 909 311
Fragments arranged by size:
3935 2174 909 311
StyI C'CwwG_G
Cuts at: 0 62 1081 1392 1521 1972 3094 5330 5369
Size: 62 1019 311 129 451 1122 2236 39
Cuts at: 5369 5726 5955 6002 6324 6839 7329
Size: 357 229 47 322 515 490
Fragments arranged by size:
2236 1122 1019 515 490 451 357 322
311 229 129 62 47 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 890 4166 4330 5300 5434 5798 6104 7329
Size: 890 3276 164 970 134 364 306 1225
Fragments arranged by size:
3276 1225 970 890 364 306 164 134
TatI w'GTAC_w
Cuts at: 0 558 1132 2007 3866 4178 7329
Size: 558 574 875 1859 312 3151
Fragments arranged by size:
3151 1859 875 574 558 312
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1699 1731 1832 1864 4789 4821 7329
Size: 1699 32 101 32 2925 32 2508
Fragments arranged by size:
2925 2508 1699 101 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 7177 7329
Size: 7177 152
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1232 4248 5428 5913 7329
Size: 1232 3016 1180 485 1416
Fragments arranged by size:
3016 1416 1232 1180 485
XmaI C'CCGG_G
Cuts at: 0 128 7329
Size: 128 7201
XmnI GAAnn'nnTTC
Cuts at: 0 911 7329
Size: 911 6418
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AseI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI
NspI PasI PciI PflMI PfoI PpiI PpuMI PsiI
PspOMI PstI PvuII SacI SacII SalI SapI ScaI
SexAI SfcI SfoI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AloI AscI
AsiSI AvrII BsiEI BsiWI BsmBI BsrBI BssHII BstZ17I
ClaI CspCI DrdI EagI FseI FspAI MluI NotI
PacI PmeI PmlI PshAI PspXI PsrI PvuI RsrII
SanDI SbfI SfiI SgfI SnaBI SrfI SspI SwaI
XbaI XhoI ZraI