Restriction map for
pF1KA1847
(Linear) (Six-base) MAPSORT of: KIAA1847.seq Check: 1112 from: 1 to: 1533
KIAA1847.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 45 334 588 1533
Size: 45 289 254 945
Fragments arranged by size:
945 289 254 45
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 550 1533
Size: 148 402 983
Fragments arranged by size:
983 402 148
AflIII A'CryG_T
Cuts at: 0 1268 1533
Size: 1268 265
AleI CACnn'nnGTG
Cuts at: 0 718 1533
Size: 718 815
AlwNI CAG_nnn'CTG
Cuts at: 0 337 422 886 1533
Size: 337 85 464 647
Fragments arranged by size:
647 464 337 85
ApaI G_GGCC'C
Cuts at: 0 1227 1302 1533
Size: 1227 75 231
Fragments arranged by size:
1227 231 75
ApaLI G'TGCA_C
Cuts at: 0 931 1533
Size: 931 602
AvaI C'yCGr_G
Cuts at: 0 210 1046 1533
Size: 210 836 487
Fragments arranged by size:
836 487 210
BanI G'GyrC_C
Cuts at: 0 73 273 1111 1130 1533
Size: 73 200 838 19 403
Fragments arranged by size:
838 403 200 73 19
BanII G_rGCy'C
Cuts at: 0 137 146 649 1025 1227 1302 1443 1470
Size: 137 9 503 376 202 75 141 27
Cuts at: 1470 1533
Size: 63
Fragments arranged by size:
503 376 202 141 137 75 63 27
9
BbeI G_GCGC'C
Cuts at: 0 77 1533
Size: 77 1456
BbvCI CC'TCA_GC
Cuts at: 0 763 1533
Size: 763 770
BglI GCCn_nnn'nGGC
Cuts at: 0 1240 1297 1533
Size: 1240 57 236
Fragments arranged by size:
1240 236 57
Bme1580I G_kGCm'C
Cuts at: 0 430 541 935 1227 1302 1533
Size: 430 111 394 292 75 231
Fragments arranged by size:
430 394 292 231 111 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 498 846 1533
Size: 498 348 687
Fragments arranged by size:
687 498 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 454 571 840 936 1533
Size: 37 417 117 269 96 597
Fragments arranged by size:
597 417 269 117 96 37
Bpu10I CC'TnA_GC
Cuts at: 0 643 763 1303 1469 1533
Size: 643 120 540 166 64
Fragments arranged by size:
643 540 166 120 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 298 609 1069 1533
Size: 298 311 460 464
Fragments arranged by size:
464 460 311 298
BsaHI Gr'CG_yC
Cuts at: 0 74 1408 1533
Size: 74 1334 125
Fragments arranged by size:
1334 125 74
BsaWI w'CCGG_w
Cuts at: 0 295 1533
Size: 295 1238
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 403 433 544 574 631 661 1533
Size: 403 30 111 30 57 30 872
Fragments arranged by size:
872 403 111 57 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 370 949 1160 1269 1272 1342 1533
Size: 22 348 579 211 109 3 70 191
Fragments arranged by size:
579 348 211 191 109 70 22 3
BseYI C'CCAG_C
Cuts at: 0 923 1164 1245 1346 1415 1442 1452 1533
Size: 923 241 81 101 69 27 10 81
Fragments arranged by size:
923 241 101 81 81 69 27 10
BsiHKAI G_wGCw'C
Cuts at: 0 137 146 649 935 1533
Size: 137 9 503 286 598
Fragments arranged by size:
598 503 286 137 9
Bsp1286I G_dGCh'C
Cuts at: 0 137 146 430 541 649 935 1025 1227
Size: 137 9 284 111 108 286 90 202
Cuts at: 1227 1302 1443 1470 1533
Size: 75 141 27 63
Fragments arranged by size:
286 284 202 141 137 111 108 90
75 63 27 9
BspEI T'CCGG_A
Cuts at: 0 295 1533
Size: 295 1238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 45 113 170 334 572 588 910 1533
Size: 45 68 57 164 238 16 322 623
Fragments arranged by size:
623 322 238 164 68 57 45 16
BsrFI r'CCGG_y
Cuts at: 0 76 658 1394 1477 1533
Size: 76 582 736 83 56
Fragments arranged by size:
736 582 83 76 56
BssHII G'CGCG_C
Cuts at: 0 404 1533
Size: 404 1129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 456 873 1533
Size: 456 417 660
Fragments arranged by size:
660 456 417
BtgI C'CryG_G
Cuts at: 0 288 449 591 773 1533
Size: 288 161 142 182 760
Fragments arranged by size:
760 288 182 161 142
BtsI GCAGTG_nn'
Cuts at: 0 802 1533
Size: 802 731
EaeI y'GGCC_r
Cuts at: 0 188 674 1392 1533
Size: 188 486 718 141
Fragments arranged by size:
718 486 188 141
EarI CTCTTCn'nnn_
Cuts at: 0 169 197 356 374 461 1134 1321 1533
Size: 169 28 159 18 87 673 187 212
Fragments arranged by size:
673 212 187 169 159 87 28 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 487 1533
Size: 487 1046
EcoICRI GAG'CTC
Cuts at: 0 135 144 647 1533
Size: 135 9 503 886
Fragments arranged by size:
886 503 135 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 148 454 550 571 840 936 1533
Size: 37 111 306 96 21 269 96 597
Fragments arranged by size:
597 306 269 111 96 96 37 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1486 1533
Size: 1486 47
EcoO109I rG'GnC_Cy
Cuts at: 0 123 633 899 1223 1224 1299 1533
Size: 123 510 266 324 1 75 234
Fragments arranged by size:
510 324 266 234 123 75 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 582 1533
Size: 582 951
FspI TGC'GCA
Cuts at: 0 44 808 1533
Size: 44 764 725
Fragments arranged by size:
764 725 44
HaeII r_GCGC'y
Cuts at: 0 77 210 1449 1533
Size: 77 133 1239 84
Fragments arranged by size:
1239 133 84 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 631 663 1533
Size: 631 32 870
Fragments arranged by size:
870 631 32
KasI G'GCGC_C
Cuts at: 0 73 1533
Size: 73 1460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 851 1533
Size: 851 682
MscI TGG'CCA
Cuts at: 0 676 1533
Size: 676 857
MslI CAynn'nnrTG
Cuts at: 0 454 686 718 967 1533
Size: 454 232 32 249 566
Fragments arranged by size:
566 454 249 232 32
MspA1I CmG'CkG
Cuts at: 0 48 114 283 1249 1350 1419 1452 1533
Size: 48 66 169 966 101 69 33 81
Fragments arranged by size:
966 169 101 81 69 66 48 33
NaeI GCC'GGC
Cuts at: 0 78 660 1396 1479 1533
Size: 78 582 736 83 54
Fragments arranged by size:
736 582 83 78 54
NarI GG'CG_CC
Cuts at: 0 74 1533
Size: 74 1459
NcoI C'CATG_G
Cuts at: 0 449 591 1533
Size: 449 142 942
Fragments arranged by size:
942 449 142
NgoMIV G'CCGG_C
Cuts at: 0 76 658 1394 1477 1533
Size: 76 582 736 83 56
Fragments arranged by size:
736 582 83 76 56
NspI r_CATG'y
Cuts at: 0 1272 1533
Size: 1272 261
PciI A'CATG_T
Cuts at: 0 1268 1533
Size: 1268 265
PflMI CCAn_nnn'nTGG
Cuts at: 0 1035 1071 1533
Size: 1035 36 462
Fragments arranged by size:
1035 462 36
PfoI T'CCnGG_A
Cuts at: 0 245 548 629 1367 1533
Size: 245 303 81 738 166
Fragments arranged by size:
738 303 245 166 81
PpuMI rG'GwC_Cy
Cuts at: 0 123 633 899 1533
Size: 123 510 266 634
Fragments arranged by size:
634 510 266 123
PspOMI G'GGCC_C
Cuts at: 0 1223 1298 1533
Size: 1223 75 235
Fragments arranged by size:
1223 235 75
PspXI vC'TCGA_Gb
Cuts at: 0 1046 1533
Size: 1046 487
PstI C_TGCA'G
Cuts at: 0 53 119 583 656 907 1085 1424 1487
Size: 53 66 464 73 251 178 339 63
Cuts at: 1487 1533
Size: 46
Fragments arranged by size:
464 339 251 178 73 66 63 53
46
PvuII CAG'CTG
Cuts at: 0 48 114 1419 1452 1533
Size: 48 66 1305 33 81
Fragments arranged by size:
1305 81 66 48 33
SacI G_AGCT'C
Cuts at: 0 137 146 649 1533
Size: 137 9 503 884
Fragments arranged by size:
884 503 137 9
SapI GCTCTTCn'nnn_
Cuts at: 0 169 197 374 1321 1533
Size: 169 28 177 947 212
Fragments arranged by size:
947 212 177 169 28
SbfI CC_TGCA'GG
Cuts at: 0 656 1533
Size: 656 877
SfcI C'TryA_G
Cuts at: 0 49 115 414 579 652 903 1081 1420
Size: 49 66 299 165 73 251 178 339
Cuts at: 1420 1483 1533
Size: 63 50
Fragments arranged by size:
339 299 251 178 165 73 66 63
50 49
SfoI GGC'GCC
Cuts at: 0 75 1533
Size: 75 1458
SmaI CCC'GGG
Cuts at: 0 212 1533
Size: 212 1321
SmlI C'TyrA_G
Cuts at: 0 1046 1533
Size: 1046 487
SrfI GCCC'GGGC
Cuts at: 0 212 1533
Size: 212 1321
StuI AGG'CCT
Cuts at: 0 1152 1533
Size: 1152 381
StyI C'CwwG_G
Cuts at: 0 68 449 591 1533
Size: 68 381 142 942
Fragments arranged by size:
942 381 142 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1525 1533
Size: 1525 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 544 576 1533
Size: 544 32 957
Fragments arranged by size:
957 544 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1032 1533
Size: 1032 501
XhoI C'TCGA_G
Cuts at: 0 1046 1533
Size: 1046 487
XmaI C'CCGG_G
Cuts at: 0 210 1533
Size: 210 1323
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbvCI BglI Bme1580I BplI BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspEI BspMI BsrFI BssHII BstXI BtgI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PpuMI PspOMI PspXI PstI PvuII SacI SapI SbfI
SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII
TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsgI BsiEI
BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI
SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SspI SwaI TatI Tth111I XbaI XmnI ZraI