Restriction map for
pF1KSDA1848
(Linear) (Six-base) MAPSORT of: KIAA1848.seq Check: 3795 from: 1 to: 1212
KIAA1848.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 767 1212
Size: 767 445
AccI GT'mk_AC
Cuts at: 0 1118 1212
Size: 1118 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1212
Size: 547 665
AflIII A'CryG_T
Cuts at: 0 559 1212
Size: 559 653
AleI CACnn'nnGTG
Cuts at: 0 748 880 1212
Size: 748 132 332
Fragments arranged by size:
748 332 132
AlwNI CAG_nnn'CTG
Cuts at: 0 361 1212
Size: 361 851
ApoI r'AATT_y
Cuts at: 0 76 1212
Size: 76 1136
AvaI C'yCGr_G
Cuts at: 0 50 131 209 1046 1212
Size: 50 81 78 837 166
Fragments arranged by size:
837 166 81 78 50
BanI G'GyrC_C
Cuts at: 0 576 859 874 886 957 1212
Size: 576 283 15 12 71 255
Fragments arranged by size:
576 283 255 71 15 12
BanII G_rGCy'C
Cuts at: 0 674 899 1109 1212
Size: 674 225 210 103
Fragments arranged by size:
674 225 210 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 100 343 463 1212
Size: 100 243 120 749
Fragments arranged by size:
749 243 120 100
BclI T'GATC_A
Cuts at: 0 344 1212
Size: 344 868
BglI GCCn_nnn'nGGC
Cuts at: 0 976 1096 1130 1212
Size: 976 120 34 82
Fragments arranged by size:
976 120 82 34
BglII A'GATC_T
Cuts at: 0 164 1212
Size: 164 1048
BlpI GC'TnA_GC
Cuts at: 0 1207 1212
Size: 1207 5
Bme1580I G_kGCm'C
Cuts at: 0 210 889 962 1212
Size: 210 679 73 250
Fragments arranged by size:
679 250 210 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 197 382 1212
Size: 197 185 830
Fragments arranged by size:
830 197 185
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 918 1212
Size: 918 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 748 1212
Size: 463 285 464
Fragments arranged by size:
464 463 285
Bpu10I CC'TnA_GC
Cuts at: 0 913 1212
Size: 913 299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 240 1212
Size: 189 51 972
Fragments arranged by size:
972 189 51
BsaAI yAC'GTr
Cuts at: 0 560 750 1212
Size: 560 190 462
Fragments arranged by size:
560 462 190
BsaHI Gr'CG_yC
Cuts at: 0 25 495 1212
Size: 25 470 717
Fragments arranged by size:
717 470 25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 211 241 391 421 560 590 1212
Size: 211 30 150 30 139 30 622
Fragments arranged by size:
622 211 150 139 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 232 472 1212
Size: 85 147 240 740
Fragments arranged by size:
740 240 147 85
BseYI C'CCAG_C
Cuts at: 0 312 843 1212
Size: 312 531 369
Fragments arranged by size:
531 369 312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 1212
Size: 79 1133
BsiHKAI G_wGCw'C
Cuts at: 0 674 1055 1109 1212
Size: 674 381 54 103
Fragments arranged by size:
674 381 103 54
BsmBI CGTCTCn'nnnn_
Cuts at: 0 724 1212
Size: 724 488
Bsp1286I G_dGCh'C
Cuts at: 0 210 674 889 899 962 1055 1109 1212
Size: 210 464 215 10 63 93 54 103
Fragments arranged by size:
464 215 210 103 93 63 54 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 767 1212
Size: 767 445
BsrFI r'CCGG_y
Cuts at: 0 491 698 1212
Size: 491 207 514
Fragments arranged by size:
514 491 207
BssSI C'ACGA_G
Cuts at: 0 772 1212
Size: 772 440
BstEII G'GTnAC_C
Cuts at: 0 261 1212
Size: 261 951
BstXI CCAn_nnnn'nTGG
Cuts at: 0 312 1212
Size: 312 900
BstYI r'GATC_y
Cuts at: 0 164 1212
Size: 164 1048
BtgI C'CryG_G
Cuts at: 0 380 1212
Size: 380 832
BtsI GCAGTG_nn'
Cuts at: 0 932 1086 1212
Size: 932 154 126
Fragments arranged by size:
932 154 126
DrdI GACnn_nn'nnGTC
Cuts at: 0 247 1212
Size: 247 965
EaeI y'GGCC_r
Cuts at: 0 81 302 620 941 968 1212
Size: 81 221 318 321 27 244
Fragments arranged by size:
321 318 244 221 81 27
EarI CTCTTCn'nnn_
Cuts at: 0 1004 1212
Size: 1004 208
EcoICRI GAG'CTC
Cuts at: 0 672 1107 1212
Size: 672 435 105
Fragments arranged by size:
672 435 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 547 748 1212
Size: 463 84 201 464
Fragments arranged by size:
464 463 201 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1009 1212
Size: 1009 203
EcoO109I rG'GnC_Cy
Cuts at: 0 249 1034 1182 1212
Size: 249 785 148 30
Fragments arranged by size:
785 249 148 30
FspI TGC'GCA
Cuts at: 0 433 1212
Size: 433 779
HaeII r_GCGC'y
Cuts at: 0 629 1212
Size: 629 583
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 585 617 1212
Size: 585 32 595
Fragments arranged by size:
595 585 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 1212
Size: 8 1204
MscI TGG'CCA
Cuts at: 0 83 970 1212
Size: 83 887 242
Fragments arranged by size:
887 242 83
MslI CAynn'nnrTG
Cuts at: 0 748 880 1212
Size: 748 132 332
Fragments arranged by size:
748 332 132
MspA1I CmG'CkG
Cuts at: 0 382 843 1212
Size: 382 461 369
Fragments arranged by size:
461 382 369
NspI r_CATG'y
Cuts at: 0 825 1212
Size: 825 387
PasI CC'CwG_GG
Cuts at: 0 855 1212
Size: 855 357
PflMI CCAn_nnn'nTGG
Cuts at: 0 152 825 1212
Size: 152 673 387
Fragments arranged by size:
673 387 152
PfoI T'CCnGG_A
Cuts at: 0 440 1212
Size: 440 772
PmlI CAC'GTG
Cuts at: 0 560 750 1212
Size: 560 190 462
Fragments arranged by size:
560 462 190
PpuMI rG'GwC_Cy
Cuts at: 0 249 1034 1182 1212
Size: 249 785 148 30
Fragments arranged by size:
785 249 148 30
PstI C_TGCA'G
Cuts at: 0 194 546 1212
Size: 194 352 666
Fragments arranged by size:
666 352 194
PvuII CAG'CTG
Cuts at: 0 843 1212
Size: 843 369
SacI G_AGCT'C
Cuts at: 0 674 1109 1212
Size: 674 435 103
Fragments arranged by size:
674 435 103
SacII CC_GC'GG
Cuts at: 0 383 1212
Size: 383 829
SanDI GG'GwC_CC
Cuts at: 0 1034 1212
Size: 1034 178
SfcI C'TryA_G
Cuts at: 0 190 542 1119 1212
Size: 190 352 577 93
Fragments arranged by size:
577 352 190 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 976 1212
Size: 976 236
SmaI CCC'GGG
Cuts at: 0 52 133 1212
Size: 52 81 1079
Fragments arranged by size:
1079 81 52
SmlI C'TyrA_G
Cuts at: 0 168 255 1212
Size: 168 87 957
Fragments arranged by size:
957 168 87
SrfI GCCC'GGGC
Cuts at: 0 133 1212
Size: 133 1079
StuI AGG'CCT
Cuts at: 0 994 1212
Size: 994 218
StyI C'CwwG_G
Cuts at: 0 536 1212
Size: 536 676
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 942 1212
Size: 942 270
TatI w'GTAC_w
Cuts at: 0 287 1212
Size: 287 925
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 560 592 1212
Size: 391 32 137 32 620
Fragments arranged by size:
620 391 137 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 210 951 1212
Size: 210 741 261
Fragments arranged by size:
741 261 210
XmaI C'CCGG_G
Cuts at: 0 50 131 1212
Size: 50 81 1081
Fragments arranged by size:
1081 81 50
XmnI GAAnn'nnTTC
Cuts at: 0 122 1212
Size: 122 1090
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI ApoI AvaI
BanI BanII BbsI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI
BssSI BstEII BstXI BstYI BtgI BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
MmeI MscI MslI MspA1I NspI PasI PflMI PfoI
PmlI PpuMI PstI PvuII SacI SacII SanDI SfcI
SfiI SmaI SmlI SrfI StuI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BfrBI BmgBI BmtI
BsaI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EciI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI
SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI
SpeI SphI SspI SwaI Tth111I XbaI XhoI ZraI