(Linear) (Six-base) MAPSORT of: KIAA1848.seq Check: 3795 from: 1 to: 1212 KIAA1848.orf With 182 enzymes: SgfI * October 24, 2006 15:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 767 1212 Size: 767 445 AccI GT'mk_AC Cuts at: 0 1118 1212 Size: 1118 94 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 547 1212 Size: 547 665 AflIII A'CryG_T Cuts at: 0 559 1212 Size: 559 653 AleI CACnn'nnGTG Cuts at: 0 748 880 1212 Size: 748 132 332 Fragments arranged by size: 748 332 132 AlwNI CAG_nnn'CTG Cuts at: 0 361 1212 Size: 361 851 ApoI r'AATT_y Cuts at: 0 76 1212 Size: 76 1136 AvaI C'yCGr_G Cuts at: 0 50 131 209 1046 1212 Size: 50 81 78 837 166 Fragments arranged by size: 837 166 81 78 50 BanI G'GyrC_C Cuts at: 0 576 859 874 886 957 1212 Size: 576 283 15 12 71 255 Fragments arranged by size: 576 283 255 71 15 12 BanII G_rGCy'C Cuts at: 0 674 899 1109 1212 Size: 674 225 210 103 Fragments arranged by size: 674 225 210 103 BbsI GAAGACnn'nnnn_ Cuts at: 0 100 343 463 1212 Size: 100 243 120 749 Fragments arranged by size: 749 243 120 100 BclI T'GATC_A Cuts at: 0 344 1212 Size: 344 868 BglI GCCn_nnn'nGGC Cuts at: 0 976 1096 1130 1212 Size: 976 120 34 82 Fragments arranged by size: 976 120 82 34 BglII A'GATC_T Cuts at: 0 164 1212 Size: 164 1048 BlpI GC'TnA_GC Cuts at: 0 1207 1212 Size: 1207 5 Bme1580I G_kGCm'C Cuts at: 0 210 889 962 1212 Size: 210 679 73 250 Fragments arranged by size: 679 250 210 73 BmrI ACTGGGnnnn_n' Cuts at: 0 197 382 1212 Size: 197 185 830 Fragments arranged by size: 830 197 185 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 918 1212 Size: 918 294 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 463 748 1212 Size: 463 285 464 Fragments arranged by size: 464 463 285 Bpu10I CC'TnA_GC Cuts at: 0 913 1212 Size: 913 299 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 189 240 1212 Size: 189 51 972 Fragments arranged by size: 972 189 51 BsaAI yAC'GTr Cuts at: 0 560 750 1212 Size: 560 190 462 Fragments arranged by size: 560 462 190 BsaHI Gr'CG_yC Cuts at: 0 25 495 1212 Size: 25 470 717 Fragments arranged by size: 717 470 25 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 211 241 391 421 560 590 1212 Size: 211 30 150 30 139 30 622 Fragments arranged by size: 622 211 150 139 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 85 232 472 1212 Size: 85 147 240 740 Fragments arranged by size: 740 240 147 85 BseYI C'CCAG_C Cuts at: 0 312 843 1212 Size: 312 531 369 Fragments arranged by size: 531 369 312 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 1212 Size: 79 1133 BsiHKAI G_wGCw'C Cuts at: 0 674 1055 1109 1212 Size: 674 381 54 103 Fragments arranged by size: 674 381 103 54 BsmBI CGTCTCn'nnnn_ Cuts at: 0 724 1212 Size: 724 488 Bsp1286I G_dGCh'C Cuts at: 0 210 674 889 899 962 1055 1109 1212 Size: 210 464 215 10 63 93 54 103 Fragments arranged by size: 464 215 210 103 93 63 54 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 767 1212 Size: 767 445 BsrFI r'CCGG_y Cuts at: 0 491 698 1212 Size: 491 207 514 Fragments arranged by size: 514 491 207 BssSI C'ACGA_G Cuts at: 0 772 1212 Size: 772 440 BstEII G'GTnAC_C Cuts at: 0 261 1212 Size: 261 951 BstXI CCAn_nnnn'nTGG Cuts at: 0 312 1212 Size: 312 900 BstYI r'GATC_y Cuts at: 0 164 1212 Size: 164 1048 BtgI C'CryG_G Cuts at: 0 380 1212 Size: 380 832 BtsI GCAGTG_nn' Cuts at: 0 932 1086 1212 Size: 932 154 126 Fragments arranged by size: 932 154 126 DrdI GACnn_nn'nnGTC Cuts at: 0 247 1212 Size: 247 965 EaeI y'GGCC_r Cuts at: 0 81 302 620 941 968 1212 Size: 81 221 318 321 27 244 Fragments arranged by size: 321 318 244 221 81 27 EarI CTCTTCn'nnn_ Cuts at: 0 1004 1212 Size: 1004 208 EcoICRI GAG'CTC Cuts at: 0 672 1107 1212 Size: 672 435 105 Fragments arranged by size: 672 435 105 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 463 547 748 1212 Size: 463 84 201 464 Fragments arranged by size: 464 463 201 84 EcoNI CCTnn'n_nnAGG Cuts at: 0 1009 1212 Size: 1009 203 EcoO109I rG'GnC_Cy Cuts at: 0 249 1034 1182 1212 Size: 249 785 148 30 Fragments arranged by size: 785 249 148 30 FspI TGC'GCA Cuts at: 0 433 1212 Size: 433 779 HaeII r_GCGC'y Cuts at: 0 629 1212 Size: 629 583 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 585 617 1212 Size: 585 32 595 Fragments arranged by size: 595 585 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 8 1212 Size: 8 1204 MscI TGG'CCA Cuts at: 0 83 970 1212 Size: 83 887 242 Fragments arranged by size: 887 242 83 MslI CAynn'nnrTG Cuts at: 0 748 880 1212 Size: 748 132 332 Fragments arranged by size: 748 332 132 MspA1I CmG'CkG Cuts at: 0 382 843 1212 Size: 382 461 369 Fragments arranged by size: 461 382 369 NspI r_CATG'y Cuts at: 0 825 1212 Size: 825 387 PasI CC'CwG_GG Cuts at: 0 855 1212 Size: 855 357 PflMI CCAn_nnn'nTGG Cuts at: 0 152 825 1212 Size: 152 673 387 Fragments arranged by size: 673 387 152 PfoI T'CCnGG_A Cuts at: 0 440 1212 Size: 440 772 PmlI CAC'GTG Cuts at: 0 560 750 1212 Size: 560 190 462 Fragments arranged by size: 560 462 190 PpuMI rG'GwC_Cy Cuts at: 0 249 1034 1182 1212 Size: 249 785 148 30 Fragments arranged by size: 785 249 148 30 PstI C_TGCA'G Cuts at: 0 194 546 1212 Size: 194 352 666 Fragments arranged by size: 666 352 194 PvuII CAG'CTG Cuts at: 0 843 1212 Size: 843 369 SacI G_AGCT'C Cuts at: 0 674 1109 1212 Size: 674 435 103 Fragments arranged by size: 674 435 103 SacII CC_GC'GG Cuts at: 0 383 1212 Size: 383 829 SanDI GG'GwC_CC Cuts at: 0 1034 1212 Size: 1034 178 SfcI C'TryA_G Cuts at: 0 190 542 1119 1212 Size: 190 352 577 93 Fragments arranged by size: 577 352 190 93 SfiI GGCCn_nnn'nGGCC Cuts at: 0 976 1212 Size: 976 236 SmaI CCC'GGG Cuts at: 0 52 133 1212 Size: 52 81 1079 Fragments arranged by size: 1079 81 52 SmlI C'TyrA_G Cuts at: 0 168 255 1212 Size: 168 87 957 Fragments arranged by size: 957 168 87 SrfI GCCC'GGGC Cuts at: 0 133 1212 Size: 133 1079 StuI AGG'CCT Cuts at: 0 994 1212 Size: 994 218 StyI C'CwwG_G Cuts at: 0 536 1212 Size: 536 676 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 942 1212 Size: 942 270 TatI w'GTAC_w Cuts at: 0 287 1212 Size: 287 925 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 391 423 560 592 1212 Size: 391 32 137 32 620 Fragments arranged by size: 620 391 137 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 210 951 1212 Size: 210 741 261 Fragments arranged by size: 741 261 210 XmaI C'CCGG_G Cuts at: 0 50 131 1212 Size: 50 81 1081 Fragments arranged by size: 1081 81 50 XmnI GAAnn'nnTTC Cuts at: 0 122 1212 Size: 122 1090 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlwNI ApoI AvaI BanI BanII BbsI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstEII BstXI BstYI BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I MmeI MscI MslI MspA1I NspI PasI PflMI PfoI PmlI PpuMI PstI PvuII SacI SacII SanDI SfcI SfiI SmaI SmlI SrfI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BmgBI BmtI BsaI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EciI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI ZraI