Restriction map for pF1KSDA1848
 (Linear) (Six-base) MAPSORT of: KIAA1848.seq  Check: 3795  from: 1  to: 1212



KIAA1848.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     767    1212 
   Size:        767     445
 
AccI GT'mk_AC

Cuts at:      0    1118    1212 
   Size:       1118      94
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547    1212 
   Size:        547     665
 
AflIII A'CryG_T

Cuts at:      0     559    1212 
   Size:        559     653
 
AleI CACnn'nnGTG

Cuts at:      0     748     880    1212 
   Size:        748     132     332

  Fragments arranged by size: 

                 748     332     132
 
AlwNI CAG_nnn'CTG

Cuts at:      0     361    1212 
   Size:        361     851
 
ApoI r'AATT_y

Cuts at:      0      76    1212 
   Size:         76    1136
 
AvaI C'yCGr_G

Cuts at:      0      50     131     209    1046    1212 
   Size:         50      81      78     837     166

  Fragments arranged by size: 

                 837     166      81      78      50
 
BanI G'GyrC_C

Cuts at:      0     576     859     874     886     957    1212 
   Size:        576     283      15      12      71     255

  Fragments arranged by size: 

                 576     283     255      71      15      12
 
BanII G_rGCy'C

Cuts at:      0     674     899    1109    1212 
   Size:        674     225     210     103

  Fragments arranged by size: 

                 674     225     210     103
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     100     343     463    1212 
   Size:        100     243     120     749

  Fragments arranged by size: 

                 749     243     120     100
 
BclI T'GATC_A

Cuts at:      0     344    1212 
   Size:        344     868
 
BglI GCCn_nnn'nGGC

Cuts at:      0     976    1096    1130    1212 
   Size:        976     120      34      82

  Fragments arranged by size: 

                 976     120      82      34
 
BglII A'GATC_T

Cuts at:      0     164    1212 
   Size:        164    1048
 
BlpI GC'TnA_GC

Cuts at:      0    1207    1212 
   Size:       1207       5
 
Bme1580I G_kGCm'C

Cuts at:      0     210     889     962    1212 
   Size:        210     679      73     250

  Fragments arranged by size: 

                 679     250     210      73
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     197     382    1212 
   Size:        197     185     830

  Fragments arranged by size: 

                 830     197     185
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     918    1212 
   Size:        918     294
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     748    1212 
   Size:        463     285     464

  Fragments arranged by size: 

                 464     463     285
 
Bpu10I CC'TnA_GC

Cuts at:      0     913    1212 
   Size:        913     299
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     240    1212 
   Size:        189      51     972

  Fragments arranged by size: 

                 972     189      51
 
BsaAI yAC'GTr

Cuts at:      0     560     750    1212 
   Size:        560     190     462

  Fragments arranged by size: 

                 560     462     190
 
BsaHI Gr'CG_yC

Cuts at:      0      25     495    1212 
   Size:         25     470     717

  Fragments arranged by size: 

                 717     470      25
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     211     241     391     421     560     590    1212 
   Size:        211      30     150      30     139      30     622

  Fragments arranged by size: 

                 622     211     150     139      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     232     472    1212 
   Size:         85     147     240     740

  Fragments arranged by size: 

                 740     240     147      85
 
BseYI C'CCAG_C

Cuts at:      0     312     843    1212 
   Size:        312     531     369

  Fragments arranged by size: 

                 531     369     312
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79    1212 
   Size:         79    1133
 
BsiHKAI G_wGCw'C

Cuts at:      0     674    1055    1109    1212 
   Size:        674     381      54     103

  Fragments arranged by size: 

                 674     381     103      54
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     724    1212 
   Size:        724     488
 
Bsp1286I G_dGCh'C

Cuts at:      0     210     674     889     899     962    1055    1109    1212 
   Size:        210     464     215      10      63      93      54     103

  Fragments arranged by size: 

                 464     215     210     103      93      63      54      10
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     767    1212 
   Size:        767     445
 
BsrFI r'CCGG_y

Cuts at:      0     491     698    1212 
   Size:        491     207     514

  Fragments arranged by size: 

                 514     491     207
 
BssSI C'ACGA_G

Cuts at:      0     772    1212 
   Size:        772     440
 
BstEII G'GTnAC_C

Cuts at:      0     261    1212 
   Size:        261     951
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     312    1212 
   Size:        312     900
 
BstYI r'GATC_y

Cuts at:      0     164    1212 
   Size:        164    1048
 
BtgI C'CryG_G

Cuts at:      0     380    1212 
   Size:        380     832
 
BtsI GCAGTG_nn'

Cuts at:      0     932    1086    1212 
   Size:        932     154     126

  Fragments arranged by size: 

                 932     154     126
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     247    1212 
   Size:        247     965
 
EaeI y'GGCC_r

Cuts at:      0      81     302     620     941     968    1212 
   Size:         81     221     318     321      27     244

  Fragments arranged by size: 

                 321     318     244     221      81      27
 
EarI CTCTTCn'nnn_

Cuts at:      0    1004    1212 
   Size:       1004     208
 
EcoICRI GAG'CTC

Cuts at:      0     672    1107    1212 
   Size:        672     435     105

  Fragments arranged by size: 

                 672     435     105
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     547     748    1212 
   Size:        463      84     201     464

  Fragments arranged by size: 

                 464     463     201      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1009    1212 
   Size:       1009     203
 
EcoO109I rG'GnC_Cy

Cuts at:      0     249    1034    1182    1212 
   Size:        249     785     148      30

  Fragments arranged by size: 

                 785     249     148      30
 
FspI TGC'GCA

Cuts at:      0     433    1212 
   Size:        433     779
 
HaeII r_GCGC'y

Cuts at:      0     629    1212 
   Size:        629     583
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     585     617    1212 
   Size:        585      32     595

  Fragments arranged by size: 

                 595     585      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0       8    1212 
   Size:          8    1204
 
MscI TGG'CCA

Cuts at:      0      83     970    1212 
   Size:         83     887     242

  Fragments arranged by size: 

                 887     242      83
 
MslI CAynn'nnrTG

Cuts at:      0     748     880    1212 
   Size:        748     132     332

  Fragments arranged by size: 

                 748     332     132
 
MspA1I CmG'CkG

Cuts at:      0     382     843    1212 
   Size:        382     461     369

  Fragments arranged by size: 

                 461     382     369
 
NspI r_CATG'y

Cuts at:      0     825    1212 
   Size:        825     387
 
PasI CC'CwG_GG

Cuts at:      0     855    1212 
   Size:        855     357
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     152     825    1212 
   Size:        152     673     387

  Fragments arranged by size: 

                 673     387     152
 
PfoI T'CCnGG_A

Cuts at:      0     440    1212 
   Size:        440     772
 
PmlI CAC'GTG

Cuts at:      0     560     750    1212 
   Size:        560     190     462

  Fragments arranged by size: 

                 560     462     190
 
PpuMI rG'GwC_Cy

Cuts at:      0     249    1034    1182    1212 
   Size:        249     785     148      30

  Fragments arranged by size: 

                 785     249     148      30
 
PstI C_TGCA'G

Cuts at:      0     194     546    1212 
   Size:        194     352     666

  Fragments arranged by size: 

                 666     352     194
 
PvuII CAG'CTG

Cuts at:      0     843    1212 
   Size:        843     369
 
SacI G_AGCT'C

Cuts at:      0     674    1109    1212 
   Size:        674     435     103

  Fragments arranged by size: 

                 674     435     103
 
SacII CC_GC'GG

Cuts at:      0     383    1212 
   Size:        383     829
 
SanDI GG'GwC_CC

Cuts at:      0    1034    1212 
   Size:       1034     178
 
SfcI C'TryA_G

Cuts at:      0     190     542    1119    1212 
   Size:        190     352     577      93

  Fragments arranged by size: 

                 577     352     190      93
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     976    1212 
   Size:        976     236
 
SmaI CCC'GGG

Cuts at:      0      52     133    1212 
   Size:         52      81    1079

  Fragments arranged by size: 

                1079      81      52
 
SmlI C'TyrA_G

Cuts at:      0     168     255    1212 
   Size:        168      87     957

  Fragments arranged by size: 

                 957     168      87
 
SrfI GCCC'GGGC

Cuts at:      0     133    1212 
   Size:        133    1079
 
StuI AGG'CCT

Cuts at:      0     994    1212 
   Size:        994     218
 
StyI C'CwwG_G

Cuts at:      0     536    1212 
   Size:        536     676
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     942    1212 
   Size:        942     270
 
TatI w'GTAC_w

Cuts at:      0     287    1212 
   Size:        287     925
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     391     423     560     592    1212 
   Size:        391      32     137      32     620

  Fragments arranged by size: 

                 620     391     137      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     210     951    1212 
   Size:        210     741     261

  Fragments arranged by size: 

                 741     261     210
 
XmaI C'CCGG_G

Cuts at:      0      50     131    1212 
   Size:         50      81    1081

  Fragments arranged by size: 

                1081      81      50
 
XmnI GAAnn'nnTTC

Cuts at:      0     122    1212 
   Size:        122    1090
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI    AlwNI     ApoI     AvaI
     BanI    BanII     BbsI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI
    BssSI   BstEII    BstXI    BstYI     BtgI     BtsI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I
     MmeI     MscI     MslI   MspA1I     NspI     PasI    PflMI     PfoI
     PmlI    PpuMI     PstI    PvuII     SacI    SacII    SanDI     SfcI
     SfiI     SmaI     SmlI     SrfI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI    BfrBI    BmgBI     BmtI
     BsaI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoRI
    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SalI
     SapI     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SspI     SwaI  Tth111I     XbaI     XhoI     ZraI
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