Restriction map for
pF1KA1851
(Linear) (Six-base) MAPSORT of: KIAA1851.seq Check: 2733 from: 1 to: 1512
KIAA1851.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 536 560 695 1512
Size: 536 24 135 817
Fragments arranged by size:
817 536 135 24
Acc65I G'GTAC_C
Cuts at: 0 765 1512
Size: 765 747
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1477 1512
Size: 1477 35
AfeI AGC'GCT
Cuts at: 0 697 1512
Size: 697 815
AflIII A'CryG_T
Cuts at: 0 356 1512
Size: 356 1156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1006 1512
Size: 1006 506
AlwNI CAG_nnn'CTG
Cuts at: 0 225 1417 1512
Size: 225 1192 95
Fragments arranged by size:
1192 225 95
ApaI G_GGCC'C
Cuts at: 0 1070 1512
Size: 1070 442
ApaLI G'TGCA_C
Cuts at: 0 436 1111 1512
Size: 436 675 401
Fragments arranged by size:
675 436 401
ApoI r'AATT_y
Cuts at: 0 1423 1512
Size: 1423 89
AvaI C'yCGr_G
Cuts at: 0 1132 1512
Size: 1132 380
AvrII C'CTAG_G
Cuts at: 0 147 1512
Size: 147 1365
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 96 129 757 790 1512
Size: 96 33 628 33 722
Fragments arranged by size:
722 628 96 33 33
BamHI G'GATC_C
Cuts at: 0 967 1512
Size: 967 545
BanI G'GyrC_C
Cuts at: 0 55 243 701 765 960 1512
Size: 55 188 458 64 195 552
Fragments arranged by size:
552 458 195 188 64 55
BanII G_rGCy'C
Cuts at: 0 1070 1133 1512
Size: 1070 63 379
Fragments arranged by size:
1070 379 63
BbeI G_GCGC'C
Cuts at: 0 247 964 1512
Size: 247 717 548
Fragments arranged by size:
717 548 247
BbsI GAAGACnn'nnnn_
Cuts at: 0 303 1512
Size: 303 1209
BbvCI CC'TCA_GC
Cuts at: 0 1418 1512
Size: 1418 94
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 160 194 274 308 486 520 1512
Size: 160 34 80 34 178 34 992
Fragments arranged by size:
992 178 160 80 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1385 1394 1512
Size: 1385 9 118
Fragments arranged by size:
1385 118 9
BlpI GC'TnA_GC
Cuts at: 0 133 475 1285 1512
Size: 133 342 810 227
Fragments arranged by size:
810 342 227 133
Bme1580I G_kGCm'C
Cuts at: 0 440 1070 1115 1512
Size: 440 630 45 397
Fragments arranged by size:
630 440 397 45
BmgBI CAC'GTC
Cuts at: 0 162 1512
Size: 162 1350
BmrI ACTGGGnnnn_n'
Cuts at: 0 1073 1512
Size: 1073 439
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 834 1512
Size: 834 678
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 424 1390 1463 1512
Size: 202 222 966 73 49
Fragments arranged by size:
966 222 202 73 49
Bpu10I CC'TnA_GC
Cuts at: 0 202 602 715 1418 1512
Size: 202 400 113 703 94
Fragments arranged by size:
703 400 202 113 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1512
Size: 613 899
BsaAI yAC'GTr
Cuts at: 0 747 1359 1512
Size: 747 612 153
Fragments arranged by size:
747 612 153
BsaHI Gr'CG_yC
Cuts at: 0 244 308 961 1512
Size: 244 64 653 551
Fragments arranged by size:
653 551 244 64
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 556 586 1512
Size: 556 30 926
Fragments arranged by size:
926 556 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 561 1512
Size: 561 951
BseYI C'CCAG_C
Cuts at: 0 18 297 393 582 828 1164 1267 1330
Size: 18 279 96 189 246 336 103 63
Cuts at: 1330 1512
Size: 182
Fragments arranged by size:
336 279 246 189 182 103 96 63
18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 253 514 1060 1423 1512
Size: 123 130 261 546 363 89
Fragments arranged by size:
546 363 261 130 123 89
BsiEI CG_ry'CG
Cuts at: 0 850 1512
Size: 850 662
BsiHKAI G_wGCw'C
Cuts at: 0 440 860 1115 1190 1512
Size: 440 420 255 75 322
Fragments arranged by size:
440 420 322 255 75
Bsp1286I G_dGCh'C
Cuts at: 0 440 860 1070 1115 1133 1190 1512
Size: 440 420 210 45 18 57 322
Fragments arranged by size:
440 420 322 210 57 45 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 137 186 536 560 695 1512
Size: 137 49 350 24 135 817
Fragments arranged by size:
817 350 137 135 49 24
BsrBI CCG'CTC
Cuts at: 0 846 1275 1512
Size: 846 429 237
Fragments arranged by size:
846 429 237
BsrDI GCAATG_nn'
Cuts at: 0 923 1512
Size: 923 589
BsrFI r'CCGG_y
Cuts at: 0 578 618 1512
Size: 578 40 894
Fragments arranged by size:
894 578 40
BssHII G'CGCG_C
Cuts at: 0 261 468 470 736 1512
Size: 261 207 2 266 776
Fragments arranged by size:
776 266 261 207 2
BssSI C'ACGA_G
Cuts at: 0 1093 1189 1512
Size: 1093 96 323
Fragments arranged by size:
1093 323 96
BstAPI GCAn_nnn'nTGC
Cuts at: 0 162 1512
Size: 162 1350
BstXI CCAn_nnnn'nTGG
Cuts at: 0 582 1164 1512
Size: 582 582 348
Fragments arranged by size:
582 582 348
BstYI r'GATC_y
Cuts at: 0 967 1512
Size: 967 545
Bsu36I CC'TnA_GG
Cuts at: 0 331 886 1512
Size: 331 555 626
Fragments arranged by size:
626 555 331
BtgI C'CryG_G
Cuts at: 0 459 1175 1512
Size: 459 716 337
Fragments arranged by size:
716 459 337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 969 989 1512
Size: 969 20 523
Fragments arranged by size:
969 523 20
BtsI GCAGTG_nn'
Cuts at: 0 25 1159 1512
Size: 25 1134 353
Fragments arranged by size:
1134 353 25
EaeI y'GGCC_r
Cuts at: 0 372 608 847 914 995 1010 1058 1395
Size: 372 236 239 67 81 15 48 337
Cuts at: 1395 1512
Size: 117
Fragments arranged by size:
372 337 239 236 117 81 67 48
15
EagI C'GGCC_G
Cuts at: 0 847 1512
Size: 847 665
EarI CTCTTCn'nnn_
Cuts at: 0 256 848 1051 1512
Size: 256 592 203 461
Fragments arranged by size:
592 461 256 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1464 1512
Size: 1464 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 424 1390 1463 1477 1512
Size: 202 222 966 73 14 35
Fragments arranged by size:
966 222 202 73 35 14
EcoO109I rG'GnC_Cy
Cuts at: 0 711 1067 1386 1498 1512
Size: 711 356 319 112 14
Fragments arranged by size:
711 356 319 112 14
EcoRI G'AATT_C
Cuts at: 0 1423 1512
Size: 1423 89
FspI TGC'GCA
Cuts at: 0 922 1512
Size: 922 590
FspAI rTGC'GCAy
Cuts at: 0 922 1512
Size: 922 590
HaeII r_GCGC'y
Cuts at: 0 225 247 370 403 545 699 722 964
Size: 225 22 123 33 142 154 23 242
Cuts at: 964 1512
Size: 548
Fragments arranged by size:
548 242 225 154 142 123 33 23
22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 556 588 1512
Size: 334 32 190 32 924
Fragments arranged by size:
924 334 190 32 32
HincII GTy'rAC
Cuts at: 0 318 1512
Size: 318 1194
KasI G'GCGC_C
Cuts at: 0 243 960 1512
Size: 243 717 552
Fragments arranged by size:
717 552 243
KpnI G_GTAC'C
Cuts at: 0 769 1512
Size: 769 743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 1512
Size: 599 913
MscI TGG'CCA
Cuts at: 0 1012 1060 1512
Size: 1012 48 452
Fragments arranged by size:
1012 452 48
MslI CAynn'nnrTG
Cuts at: 0 988 1512
Size: 988 524
MspA1I CmG'CkG
Cuts at: 0 258 326 708 1223 1244 1253 1407 1455
Size: 258 68 382 515 21 9 154 48
Cuts at: 1455 1512
Size: 57
Fragments arranged by size:
515 382 258 154 68 57 48 21
9
NaeI GCC'GGC
Cuts at: 0 620 1512
Size: 620 892
NarI GG'CG_CC
Cuts at: 0 244 961 1512
Size: 244 717 551
Fragments arranged by size:
717 551 244
NgoMIV G'CCGG_C
Cuts at: 0 618 1512
Size: 618 894
NspI r_CATG'y
Cuts at: 0 39 454 1039 1512
Size: 39 415 585 473
Fragments arranged by size:
585 473 415 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 835 1512
Size: 835 677
PpuMI rG'GwC_Cy
Cuts at: 0 1498 1512
Size: 1498 14
PspOMI G'GGCC_C
Cuts at: 0 1066 1512
Size: 1066 446
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 625 657 1512
Size: 625 32 855
Fragments arranged by size:
855 625 32
PstI C_TGCA'G
Cuts at: 0 158 1454 1512
Size: 158 1296 58
Fragments arranged by size:
1296 158 58
PvuII CAG'CTG
Cuts at: 0 258 1407 1455 1512
Size: 258 1149 48 57
Fragments arranged by size:
1149 258 57 48
SanDI GG'GwC_CC
Cuts at: 0 1498 1512
Size: 1498 14
SapI GCTCTTCn'nnn_
Cuts at: 0 848 1512
Size: 848 664
ScaI AGT'ACT
Cuts at: 0 1304 1512
Size: 1304 208
SexAI A'CCwGG_T
Cuts at: 0 4 1512
Size: 4 1508
SfcI C'TryA_G
Cuts at: 0 154 691 1450 1512
Size: 154 537 759 62
Fragments arranged by size:
759 537 154 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1385 1394 1512
Size: 1385 9 118
Fragments arranged by size:
1385 118 9
SfoI GGC'GCC
Cuts at: 0 245 962 1512
Size: 245 717 550
Fragments arranged by size:
717 550 245
SmlI C'TyrA_G
Cuts at: 0 628 1512
Size: 628 884
SnaBI TAC'GTA
Cuts at: 0 747 1512
Size: 747 765
SphI G_CATG'C
Cuts at: 0 39 454 1039 1512
Size: 39 415 585 473
Fragments arranged by size:
585 473 415 39
StuI AGG'CCT
Cuts at: 0 1448 1512
Size: 1448 64
StyI C'CwwG_G
Cuts at: 0 147 1512
Size: 147 1365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 562 869 1281 1344 1512
Size: 562 307 412 63 168
Fragments arranged by size:
562 412 307 168 63
TatI w'GTAC_w
Cuts at: 0 1097 1302 1512
Size: 1097 205 210
Fragments arranged by size:
1097 210 205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1034 1066 1512
Size: 1034 32 446
Fragments arranged by size:
1034 446 32
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AlfI AlwNI ApaI
ApaLI ApoI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PflMI PpuMI PspOMI PsrI PstI PvuII SanDI
SapI ScaI SexAI SfcI SfiI SfoI SmlI SnaBI
SphI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI AleI AloI
AscI AseI AsiSI BciVI BclI BfrBI BglII BmtI
BsaI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrGI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoRV FalI FseI HindIII HpaI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PvuI RsrII SacI SacII SalI SbfI
SgfI SgrAI SmaI SpeI SrfI SspI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI