Restriction map for
pF1KSDA1852
(Linear) (Six-base) MAPSORT of: KIAA1852.seq Check: 6271 from: 1 to: 2376
KIAA1852.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 299 2376
Size: 299 2077
AatII G_ACGT'C
Cuts at: 0 1222 2376
Size: 1222 1154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1377 1713 1965 2376
Size: 1377 336 252 411
Fragments arranged by size:
1377 411 336 252
AhdI GACnn_n'nnGTC
Cuts at: 0 284 2376
Size: 284 2092
AleI CACnn'nnGTG
Cuts at: 0 2024 2376
Size: 2024 352
ApoI r'AATT_y
Cuts at: 0 631 926 1401 1427 2015 2241 2376
Size: 631 295 475 26 588 226 135
Fragments arranged by size:
631 588 475 295 226 135 26
BanI G'GyrC_C
Cuts at: 0 31 2376
Size: 31 2345
BanII G_rGCy'C
Cuts at: 0 90 158 988 1237 1573 1741 2376
Size: 90 68 830 249 336 168 635
Fragments arranged by size:
830 635 336 249 168 90 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 585 2376
Size: 585 1791
BciVI GTATCCnnnnn_n'
Cuts at: 0 113 2376
Size: 113 2263
BclI T'GATC_A
Cuts at: 0 915 2376
Size: 915 1461
BfrBI ATG'CAT
Cuts at: 0 1599 2271 2376
Size: 1599 672 105
Fragments arranged by size:
1599 672 105
BglI GCCn_nnn'nGGC
Cuts at: 0 85 2376
Size: 85 2291
BlpI GC'TnA_GC
Cuts at: 0 1320 1824 2376
Size: 1320 504 552
Fragments arranged by size:
1320 552 504
Bme1580I G_kGCm'C
Cuts at: 0 36 2376
Size: 36 2340
BmgBI CAC'GTC
Cuts at: 0 198 2376
Size: 198 2178
BmrI ACTGGGnnnn_n'
Cuts at: 0 161 2376
Size: 161 2215
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 348 710 961 2376
Size: 348 362 251 1415
Fragments arranged by size:
1415 362 348 251
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 295 498 872 1251 1289 1587 1625
Size: 151 144 203 374 379 38 298 38
Cuts at: 1625 1709 1755 1961 2129 2213 2376
Size: 84 46 206 168 84 163
Fragments arranged by size:
379 374 298 206 203 168 163 151
144 84 84 46 38 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 271 2376
Size: 271 2105
BsaAI yAC'GTr
Cuts at: 0 117 2376
Size: 117 2259
BsaHI Gr'CG_yC
Cuts at: 0 1219 2376
Size: 1219 1157
BsaWI w'CCGG_w
Cuts at: 0 2093 2376
Size: 2093 283
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 156 2376
Size: 148 8 2220
Fragments arranged by size:
2220 148 8
BseYI C'CCAG_C
Cuts at: 0 159 435 2376
Size: 159 276 1941
Fragments arranged by size:
1941 276 159
BsiHKAI G_wGCw'C
Cuts at: 0 1237 1573 1741 2376
Size: 1237 336 168 635
Fragments arranged by size:
1237 635 336 168
BsmI GAATG_Cn'
Cuts at: 0 967 1601 1810 1963 2273 2376
Size: 967 634 209 153 310 103
Fragments arranged by size:
967 634 310 209 153 103
Bsp1286I G_dGCh'C
Cuts at: 0 36 90 158 988 1237 1573 1741 2376
Size: 36 54 68 830 249 336 168 635
Fragments arranged by size:
830 635 336 249 168 68 54 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 299 2376
Size: 299 2077
BsrDI GCAATG_nn'
Cuts at: 0 2084 2376
Size: 2084 292
BsrGI T'GTAC_A
Cuts at: 0 1416 2172 2376
Size: 1416 756 204
Fragments arranged by size:
1416 756 204
BstEII G'GTnAC_C
Cuts at: 0 286 774 2376
Size: 286 488 1602
Fragments arranged by size:
1602 488 286
BstYI r'GATC_y
Cuts at: 0 2059 2376
Size: 2059 317
Bsu36I CC'TnA_GG
Cuts at: 0 323 2376
Size: 323 2053
BtgI C'CryG_G
Cuts at: 0 16 73 203 358 468 2376
Size: 16 57 130 155 110 1908
Fragments arranged by size:
1908 155 130 110 57 16
BtsI GCAGTG_nn'
Cuts at: 0 1181 2304 2376
Size: 1181 1123 72
Fragments arranged by size:
1181 1123 72
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 205 240 1194 1229 1950 1985 2376
Size: 205 35 954 35 721 35 391
Fragments arranged by size:
954 721 391 205 35 35 35
DraI TTT'AAA
Cuts at: 0 1620 2376
Size: 1620 756
EaeI y'GGCC_r
Cuts at: 0 200 2376
Size: 200 2176
EarI CTCTTCn'nnn_
Cuts at: 0 215 347 2357 2376
Size: 215 132 2010 19
Fragments arranged by size:
2010 215 132 19
EcoICRI GAG'CTC
Cuts at: 0 1235 1571 1739 2376
Size: 1235 336 168 637
Fragments arranged by size:
1235 637 336 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 295 498 872 1251 1289 1377 1587
Size: 151 144 203 374 379 38 88 210
Cuts at: 1587 1625 1709 1713 1755 1961 1965 2129 2213
Size: 38 84 4 42 206 4 164 84
Cuts at: 2213 2376
Size: 163
Fragments arranged by size:
379 374 210 206 203 164 163 151
144 88 84 84 42 38 38 4
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 2376
Size: 133 2243
EcoO109I rG'GnC_Cy
Cuts at: 0 251 2376
Size: 251 2125
EcoRI G'AATT_C
Cuts at: 0 631 926 1401 1427 2015 2241 2376
Size: 631 295 475 26 588 226 135
Fragments arranged by size:
631 588 475 295 226 135 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 270 302 346 378 1560 1592 1728 1760
Size: 270 32 44 32 1182 32 136 32
Cuts at: 1760 2146 2178 2376
Size: 386 32 198
Fragments arranged by size:
1182 386 270 198 136 44 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 69 2376
Size: 69 2307
HindIII A'AGCT_T
Cuts at: 0 1640 2376
Size: 1640 736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 461 718 999 2376
Size: 320 141 257 281 1377
Fragments arranged by size:
1377 320 281 257 141
MslI CAynn'nnrTG
Cuts at: 0 911 1704 2024 2124 2376
Size: 911 793 320 100 252
Fragments arranged by size:
911 793 320 252 100
MspA1I CmG'CkG
Cuts at: 0 64 234 1397 1565 1733 2376
Size: 64 170 1163 168 168 643
Fragments arranged by size:
1163 643 170 168 168 64
NcoI C'CATG_G
Cuts at: 0 73 468 2376
Size: 73 395 1908
Fragments arranged by size:
1908 395 73
NdeI CA'TA_TG
Cuts at: 0 1589 2376
Size: 1589 787
NsiI A_TGCA'T
Cuts at: 0 1601 2273 2376
Size: 1601 672 103
Fragments arranged by size:
1601 672 103
NspI r_CATG'y
Cuts at: 0 380 730 2376
Size: 380 350 1646
Fragments arranged by size:
1646 380 350
PmlI CAC'GTG
Cuts at: 0 117 2376
Size: 117 2259
PpuMI rG'GwC_Cy
Cuts at: 0 251 2376
Size: 251 2125
PstI C_TGCA'G
Cuts at: 0 1188 2376
Size: 1188 1188
PvuII CAG'CTG
Cuts at: 0 64 234 1397 1565 1733 2376
Size: 64 170 1163 168 168 643
Fragments arranged by size:
1163 643 170 168 168 64
SacI G_AGCT'C
Cuts at: 0 1237 1573 1741 2376
Size: 1237 336 168 635
Fragments arranged by size:
1237 635 336 168
SapI GCTCTTCn'nnn_
Cuts at: 0 347 2376
Size: 347 2029
ScaI AGT'ACT
Cuts at: 0 611 2376
Size: 611 1765
SfcI C'TryA_G
Cuts at: 0 1100 1184 1896 2070 2376
Size: 1100 84 712 174 306
Fragments arranged by size:
1100 712 306 174 84
StyI C'CwwG_G
Cuts at: 0 73 468 505 1006 2376
Size: 73 395 37 501 1370
Fragments arranged by size:
1370 501 395 73 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 229 393 1019 2376
Size: 229 164 626 1357
Fragments arranged by size:
1357 626 229 164
TatI w'GTAC_w
Cuts at: 0 571 609 871 1416 1540 1708 2172 2376
Size: 571 38 262 545 124 168 464 204
Fragments arranged by size:
571 545 464 262 204 168 124 38
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 80 2376
Size: 80 2296
XmnI GAAnn'nnTTC
Cuts at: 0 1474 1558 1683 2376
Size: 1474 84 125 693
Fragments arranged by size:
1474 693 125 84
ZraI GAC'GTC
Cuts at: 0 1220 2376
Size: 1220 1156
Enzymes that do cut:
AarI AatII AcuI AhdI AleI ApoI BanI BanII
BbsI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI BsaI BsaAI BsaHI BsaWI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstEII
BstYI Bsu36I BtgI BtsI CspCI DraI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII
MmeI MslI MspA1I NcoI NdeI NsiI NspI PmlI
PpuMI PstI PvuII SacI SapI ScaI SfcI StyI
TaqII TatI XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BglII BmtI
Bpu10I BpuEI BsaBI BsaXI BsgI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstZ17I BtgZI ClaI DraIII DrdI EagI EciI
EcoRV FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XhoI XmaI