Restriction map for
pF1KSDA1857
(Linear) (Six-base) MAPSORT of: KIAA1857.seq Check: 9884 from: 1 to: 1590
KIAA1857.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 845 1411 1590
Size: 845 566 179
Fragments arranged by size:
845 566 179
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1590
Size: 157 1433
AfeI AGC'GCT
Cuts at: 0 1420 1590
Size: 1420 170
AflIII A'CryG_T
Cuts at: 0 178 551 1361 1590
Size: 178 373 810 229
Fragments arranged by size:
810 373 229 178
AgeI A'CCGG_T
Cuts at: 0 1256 1590
Size: 1256 334
AleI CACnn'nnGTG
Cuts at: 0 793 1447 1590
Size: 793 654 143
Fragments arranged by size:
793 654 143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 781 811 1423 1590
Size: 781 30 612 167
Fragments arranged by size:
781 612 167 30
AlwNI CAG_nnn'CTG
Cuts at: 0 1383 1590
Size: 1383 207
ApaI G_GGCC'C
Cuts at: 0 94 546 1306 1590
Size: 94 452 760 284
Fragments arranged by size:
760 452 284 94
ApaLI G'TGCA_C
Cuts at: 0 1249 1590
Size: 1249 341
ApoI r'AATT_y
Cuts at: 0 963 1590
Size: 963 627
AvaI C'yCGr_G
Cuts at: 0 1516 1590
Size: 1516 74
BanI G'GyrC_C
Cuts at: 0 787 1340 1399 1590
Size: 787 553 59 191
Fragments arranged by size:
787 553 191 59
BanII G_rGCy'C
Cuts at: 0 94 167 546 1306 1590
Size: 94 73 379 760 284
Fragments arranged by size:
760 379 284 94 73
BbeI G_GCGC'C
Cuts at: 0 1344 1590
Size: 1344 246
BglI GCCn_nnn'nGGC
Cuts at: 0 541 1169 1292 1436 1590
Size: 541 628 123 144 154
Fragments arranged by size:
628 541 154 144 123
Bme1580I G_kGCm'C
Cuts at: 0 94 546 1253 1306 1590
Size: 94 452 707 53 284
Fragments arranged by size:
707 452 284 94 53
BmgBI CAC'GTC
Cuts at: 0 408 1590
Size: 408 1182
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 612 1590
Size: 612 978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 472 546 730 1590
Size: 354 118 74 184 860
Fragments arranged by size:
860 354 184 118 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 1590
Size: 712 878
BsaI GGTCTCn'nnnn_
Cuts at: 0 179 1261 1590
Size: 179 1082 329
Fragments arranged by size:
1082 329 179
BsaAI yAC'GTr
Cuts at: 0 630 1590
Size: 630 960
BsaHI Gr'CG_yC
Cuts at: 0 245 1341 1590
Size: 245 1096 249
Fragments arranged by size:
1096 249 245
BsaWI w'CCGG_w
Cuts at: 0 1256 1590
Size: 1256 334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 817 847 871 901 1057 1087 1590
Size: 817 30 24 30 156 30 503
Fragments arranged by size:
817 503 156 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 313 604 1590
Size: 310 3 291 986
Fragments arranged by size:
986 310 291 3
BseYI C'CCAG_C
Cuts at: 0 346 602 780 1167 1563 1590
Size: 346 256 178 387 396 27
Fragments arranged by size:
396 387 346 256 178 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 567 817 1590
Size: 10 557 250 773
Fragments arranged by size:
773 557 250 10
BsiEI CG_ry'CG
Cuts at: 0 432 1257 1509 1590
Size: 432 825 252 81
Fragments arranged by size:
825 432 252 81
BsiHKAI G_wGCw'C
Cuts at: 0 581 889 926 1253 1590
Size: 581 308 37 327 337
Fragments arranged by size:
581 337 327 308 37
BsmI GAATG_Cn'
Cuts at: 0 1066 1590
Size: 1066 524
Bsp1286I G_dGCh'C
Cuts at: 0 94 167 546 581 889 926 1253 1306
Size: 94 73 379 35 308 37 327 53
Cuts at: 1306 1590
Size: 284
Fragments arranged by size:
379 327 308 284 94 73 53 37
35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 686 761 845 878 1007 1030 1123 1411
Size: 686 75 84 33 129 23 93 288
Cuts at: 1411 1590
Size: 179
Fragments arranged by size:
686 288 179 129 93 84 75 33
23
BsrFI r'CCGG_y
Cuts at: 0 431 667 934 988 1163 1256 1270 1444
Size: 431 236 267 54 175 93 14 174
Cuts at: 1444 1590
Size: 146
Fragments arranged by size:
431 267 236 175 174 146 93 54
14
BssHII G'CGCG_C
Cuts at: 0 566 1511 1590
Size: 566 945 79
Fragments arranged by size:
945 566 79
BstAPI GCAn_nnn'nTGC
Cuts at: 0 576 1590
Size: 576 1014
BstEII G'GTnAC_C
Cuts at: 0 75 1110 1590
Size: 75 1035 480
Fragments arranged by size:
1035 480 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 354 444 1590
Size: 354 90 1146
Fragments arranged by size:
1146 354 90
Bsu36I CC'TnA_GG
Cuts at: 0 168 1590
Size: 168 1422
BtgI C'CryG_G
Cuts at: 0 389 437 1011 1590
Size: 389 48 574 579
Fragments arranged by size:
579 574 389 48
BtsI GCAGTG_nn'
Cuts at: 0 27 916 1149 1158 1590
Size: 27 889 233 9 432
Fragments arranged by size:
889 432 233 27 9
DraIII CAC_nnn'GTG
Cuts at: 0 576 1590
Size: 576 1014
EaeI y'GGCC_r
Cuts at: 0 308 429 1571 1590
Size: 308 121 1142 19
Fragments arranged by size:
1142 308 121 19
EagI C'GGCC_G
Cuts at: 0 429 1590
Size: 429 1161
EarI CTCTTCn'nnn_
Cuts at: 0 25 1590
Size: 25 1565
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 522 1590
Size: 522 1068
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 354 472 546 730 1590
Size: 157 197 118 74 184 860
Fragments arranged by size:
860 197 184 157 118 74
EcoO109I rG'GnC_Cy
Cuts at: 0 90 91 303 723 1303 1590
Size: 90 1 212 420 580 287
Fragments arranged by size:
580 420 287 212 90 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 753 1590
Size: 753 837
FseI GG_CCGG'CC
Cuts at: 0 435 1590
Size: 435 1155
FspI TGC'GCA
Cuts at: 0 682 757 1590
Size: 682 75 833
Fragments arranged by size:
833 682 75
HaeII r_GCGC'y
Cuts at: 0 19 719 781 1344 1422 1590
Size: 19 700 62 563 78 168
Fragments arranged by size:
700 563 168 78 62 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 541 573 1590
Size: 541 32 1017
Fragments arranged by size:
1017 541 32
KasI G'GCGC_C
Cuts at: 0 1340 1590
Size: 1340 250
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 860 1100 1590
Size: 275 585 240 490
Fragments arranged by size:
585 490 275 240
MscI TGG'CCA
Cuts at: 0 310 1590
Size: 310 1280
MslI CAynn'nnrTG
Cuts at: 0 442 793 1447 1590
Size: 442 351 654 143
Fragments arranged by size:
654 442 351 143
MspA1I CmG'CkG
Cuts at: 0 354 801 1005 1036 1082 1124 1380 1469
Size: 354 447 204 31 46 42 256 89
Cuts at: 1469 1590
Size: 121
Fragments arranged by size:
447 354 256 204 121 89 46 42
31
NaeI GCC'GGC
Cuts at: 0 433 669 990 1165 1590
Size: 433 236 321 175 425
Fragments arranged by size:
433 425 321 236 175
NarI GG'CG_CC
Cuts at: 0 1341 1590
Size: 1341 249
NcoI C'CATG_G
Cuts at: 0 1011 1590
Size: 1011 579
NgoMIV G'CCGG_C
Cuts at: 0 431 667 988 1163 1590
Size: 431 236 321 175 427
Fragments arranged by size:
431 427 321 236 175
NspI r_CATG'y
Cuts at: 0 182 555 762 877 1590
Size: 182 373 207 115 713
Fragments arranged by size:
713 373 207 182 115
PciI A'CATG_T
Cuts at: 0 178 551 1590
Size: 178 373 1039
Fragments arranged by size:
1039 373 178
PflMI CCAn_nnn'nTGG
Cuts at: 0 317 1590
Size: 317 1273
PfoI T'CCnGG_A
Cuts at: 0 449 1590
Size: 449 1141
PmlI CAC'GTG
Cuts at: 0 630 1590
Size: 630 960
PshAI GACnn'nnGTC
Cuts at: 0 1117 1493 1590
Size: 1117 376 97
Fragments arranged by size:
1117 376 97
PspOMI G'GGCC_C
Cuts at: 0 90 542 1302 1590
Size: 90 452 760 288
Fragments arranged by size:
760 452 288 90
PstI C_TGCA'G
Cuts at: 0 911 1041 1087 1412 1590
Size: 911 130 46 325 178
Fragments arranged by size:
911 325 178 130 46
PvuII CAG'CTG
Cuts at: 0 1124 1380 1590
Size: 1124 256 210
Fragments arranged by size:
1124 256 210
SapI GCTCTTCn'nnn_
Cuts at: 0 25 1590
Size: 25 1565
ScaI AGT'ACT
Cuts at: 0 595 820 1590
Size: 595 225 770
Fragments arranged by size:
770 595 225
SfcI C'TryA_G
Cuts at: 0 907 1037 1083 1408 1590
Size: 907 130 46 325 182
Fragments arranged by size:
907 325 182 130 46
SfoI GGC'GCC
Cuts at: 0 1342 1590
Size: 1342 248
SgrAI Cr'CCGG_yG
Cuts at: 0 1444 1590
Size: 1444 146
SmaI CCC'GGG
Cuts at: 0 1518 1590
Size: 1518 72
SmlI C'TyrA_G
Cuts at: 0 727 1590
Size: 727 863
SphI G_CATG'C
Cuts at: 0 762 877 1590
Size: 762 115 713
Fragments arranged by size:
762 713 115
StuI AGG'CCT
Cuts at: 0 520 1590
Size: 520 1070
StyI C'CwwG_G
Cuts at: 0 122 719 1011 1590
Size: 122 597 292 579
Fragments arranged by size:
597 579 292 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 63 415 1590
Size: 63 352 1175
Fragments arranged by size:
1175 352 63
TatI w'GTAC_w
Cuts at: 0 593 818 1590
Size: 593 225 772
Fragments arranged by size:
772 593 225
Tth111I GACn'n_nGTC
Cuts at: 0 145 1590
Size: 145 1445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 1590
Size: 258 1332
XmaI C'CCGG_G
Cuts at: 0 1516 1590
Size: 1516 74
XmnI GAAnn'nnTTC
Cuts at: 0 964 1590
Size: 964 626
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BglI
Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspMI BsrFI BssHII BstAPI BstEII BstXI Bsu36I
BtgI BtsI DraIII EaeI EagI EarI EciI Eco57MI
EcoO109I FalI FseI FspI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PfoI PmlI PshAI PspOMI PstI PvuII
SapI ScaI SfcI SfoI SgrAI SmaI SmlI SphI
StuI StyI TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AhdI AloI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmrI BmtI Bpu10I
BsaBI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI
BssSI BstBI BstYI BstZ17I BtgZI ClaI CspCI DraI
DrdI EcoICRI EcoNI EcoRI EcoRV FspAI HincII HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PasI PmeI PpiI PpuMI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SgfI SnaBI SpeI SrfI SspI SwaI
TstI XbaI XhoI ZraI