Restriction map for
pF1KSDA1859
(Linear) (Six-base) MAPSORT of: KIAA1859.seq Check: 4027 from: 1 to: 2223
KIAA1859.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 715 2223
Size: 715 1508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 1339 1429 2223
Size: 366 973 90 794
Fragments arranged by size:
973 794 366 90
AfeI AGC'GCT
Cuts at: 0 1925 2223
Size: 1925 298
AlwNI CAG_nnn'CTG
Cuts at: 0 252 928 931 982 2223
Size: 252 676 3 51 1241
Fragments arranged by size:
1241 676 252 51 3
ApaI G_GGCC'C
Cuts at: 0 476 613 796 1890 1902 2223
Size: 476 137 183 1094 12 321
Fragments arranged by size:
1094 476 321 183 137 12
ApoI r'AATT_y
Cuts at: 0 112 1717 2223
Size: 112 1605 506
Fragments arranged by size:
1605 506 112
AvaI C'yCGr_G
Cuts at: 0 350 544 1176 1802 2223
Size: 350 194 632 626 421
Fragments arranged by size:
632 626 421 350 194
BamHI G'GATC_C
Cuts at: 0 273 1400 2012 2210 2223
Size: 273 1127 612 198 13
Fragments arranged by size:
1127 612 273 198 13
BanI G'GyrC_C
Cuts at: 0 136 1613 2107 2191 2223
Size: 136 1477 494 84 32
Fragments arranged by size:
1477 494 136 84 32
BanII G_rGCy'C
Cuts at: 0 218 476 613 796 1010 1149 1740 1890
Size: 218 258 137 183 214 139 591 150
Cuts at: 1890 1902 1967 2223
Size: 12 65 256
Fragments arranged by size:
591 258 256 218 214 183 150 139
137 65 12
BbeI G_GCGC'C
Cuts at: 0 2195 2223
Size: 2195 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 53 696 2223
Size: 53 643 1527
Fragments arranged by size:
1527 643 53
BbvCI CC'TCA_GC
Cuts at: 0 5 1464 2223
Size: 5 1459 759
Fragments arranged by size:
1459 759 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1861 1895 2223
Size: 1861 34 328
Fragments arranged by size:
1861 328 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 429 2223
Size: 429 1794
BglI GCCn_nnn'nGGC
Cuts at: 0 463 547 595 778 946 1561 2223
Size: 463 84 48 183 168 615 662
Fragments arranged by size:
662 615 463 183 168 84 48
BlpI GC'TnA_GC
Cuts at: 0 992 1004 2223
Size: 992 12 1219
Fragments arranged by size:
1219 992 12
Bme1580I G_kGCm'C
Cuts at: 0 476 613 796 818 1890 1902 2223
Size: 476 137 183 22 1072 12 321
Fragments arranged by size:
1072 476 321 183 137 22 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 791 2223
Size: 791 1432
BmtI G_CTAG'C
Cuts at: 0 401 2223
Size: 401 1822
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1109 2223
Size: 1109 1114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1134 2223
Size: 1134 1089
Bpu10I CC'TnA_GC
Cuts at: 0 5 467 482 962 1464 2223
Size: 5 462 15 480 502 759
Fragments arranged by size:
759 502 480 462 15 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1179 2223
Size: 1179 1044
BsaI GGTCTCn'nnnn_
Cuts at: 0 429 753 1741 1790 2223
Size: 429 324 988 49 433
Fragments arranged by size:
988 433 429 324 49
BsaAI yAC'GTr
Cuts at: 0 1203 2084 2223
Size: 1203 881 139
Fragments arranged by size:
1203 881 139
BsaBI GATnn'nnATC
Cuts at: 0 1297 1909 2223
Size: 1297 612 314
Fragments arranged by size:
1297 612 314
BsaHI Gr'CG_yC
Cuts at: 0 662 1529 2096 2192 2223
Size: 662 867 567 96 31
Fragments arranged by size:
867 662 567 96 31
BsaWI w'CCGG_w
Cuts at: 0 276 2165 2223
Size: 276 1889 58
Fragments arranged by size:
1889 276 58
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1093 1096 1452 1501 2223
Size: 1093 3 356 49 722
Fragments arranged by size:
1093 722 356 49 3
BseYI C'CCAG_C
Cuts at: 0 440 673 768 1943 2146 2223
Size: 440 233 95 1175 203 77
Fragments arranged by size:
1175 440 233 203 95 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 995 1434 2223
Size: 995 439 789
Fragments arranged by size:
995 789 439
BsiEI CG_ry'CG
Cuts at: 0 302 2223
Size: 302 1921
BsiHKAI G_wGCw'C
Cuts at: 0 1967 2223
Size: 1967 256
BsmBI CGTCTCn'nnnn_
Cuts at: 0 289 2223
Size: 289 1934
Bsp1286I G_dGCh'C
Cuts at: 0 218 476 613 796 818 1010 1149 1740
Size: 218 258 137 183 22 192 139 591
Cuts at: 1740 1890 1902 1967 2223
Size: 150 12 65 256
Fragments arranged by size:
591 258 256 218 192 183 150 139
137 65 22 12
BspEI T'CCGG_A
Cuts at: 0 276 2165 2223
Size: 276 1889 58
Fragments arranged by size:
1889 276 58
BspHI T'CATG_A
Cuts at: 0 1538 2223
Size: 1538 685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 411 715 771 2223
Size: 411 304 56 1452
Fragments arranged by size:
1452 411 304 56
BsrDI GCAATG_nn'
Cuts at: 0 262 2223
Size: 262 1961
BsrFI r'CCGG_y
Cuts at: 0 668 720 2223
Size: 668 52 1503
Fragments arranged by size:
1503 668 52
BssHII G'CGCG_C
Cuts at: 0 1305 2223
Size: 1305 918
BssSI C'ACGA_G
Cuts at: 0 951 2223
Size: 951 1272
BstAPI GCAn_nnn'nTGC
Cuts at: 0 928 931 982 2223
Size: 928 3 51 1241
Fragments arranged by size:
1241 928 51 3
BstXI CCAn_nnnn'nTGG
Cuts at: 0 534 657 1053 1112 2223
Size: 534 123 396 59 1111
Fragments arranged by size:
1111 534 396 123 59
BstYI r'GATC_y
Cuts at: 0 273 1292 1400 1688 2012 2210 2223
Size: 273 1019 108 288 324 198 13
Fragments arranged by size:
1019 324 288 273 198 108 13
BtgI C'CryG_G
Cuts at: 0 1097 2223
Size: 1097 1126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1761 2012 2223
Size: 1761 251 211
Fragments arranged by size:
1761 251 211
BtsI GCAGTG_nn'
Cuts at: 0 986 2109 2223
Size: 986 1123 114
Fragments arranged by size:
1123 986 114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1841 1876 2223
Size: 1841 35 347
Fragments arranged by size:
1841 347 35
DraIII CAC_nnn'GTG
Cuts at: 0 408 2223
Size: 408 1815
DrdI GACnn_nn'nnGTC
Cuts at: 0 867 1041 1957 2223
Size: 867 174 916 266
Fragments arranged by size:
916 867 266 174
EaeI y'GGCC_r
Cuts at: 0 299 410 884 947 1868 2223
Size: 299 111 474 63 921 355
Fragments arranged by size:
921 474 355 299 111 63
EagI C'GGCC_G
Cuts at: 0 299 2223
Size: 299 1924
EarI CTCTTCn'nnn_
Cuts at: 0 77 2223
Size: 77 2146
EcoICRI GAG'CTC
Cuts at: 0 1965 2223
Size: 1965 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 1134 1339 1429 2223
Size: 366 768 205 90 794
Fragments arranged by size:
794 768 366 205 90
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 1014 1237 1573 2007 2223
Size: 256 758 223 336 434 216
Fragments arranged by size:
758 434 336 256 223 216
EcoO109I rG'GnC_Cy
Cuts at: 0 472 609 610 792 793 826 1181 1728
Size: 472 137 1 182 1 33 355 547
Cuts at: 1728 1764 1886 1898 2223
Size: 36 122 12 325
Fragments arranged by size:
547 472 355 325 182 137 122 36
33 12 1 1
EcoRI G'AATT_C
Cuts at: 0 112 1717 2223
Size: 112 1605 506
Fragments arranged by size:
1605 506 112
HaeII r_GCGC'y
Cuts at: 0 623 883 1927 2195 2223
Size: 623 260 1044 268 28
Fragments arranged by size:
1044 623 268 260 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2081 2113 2223
Size: 2081 32 110
Fragments arranged by size:
2081 110 32
HindIII A'AGCT_T
Cuts at: 0 12 180 2223
Size: 12 168 2043
Fragments arranged by size:
2043 168 12
KasI G'GCGC_C
Cuts at: 0 2191 2223
Size: 2191 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 2223
Size: 362 1861
MscI TGG'CCA
Cuts at: 0 412 886 949 2223
Size: 412 474 63 1274
Fragments arranged by size:
1274 474 412 63
MslI CAynn'nnrTG
Cuts at: 0 1055 1537 2223
Size: 1055 482 686
Fragments arranged by size:
1055 686 482
MspA1I CmG'CkG
Cuts at: 0 270 358 367 928 979 1099 2223
Size: 270 88 9 561 51 120 1124
Fragments arranged by size:
1124 561 270 120 88 51 9
NaeI GCC'GGC
Cuts at: 0 722 2223
Size: 722 1501
NarI GG'CG_CC
Cuts at: 0 2192 2223
Size: 2192 31
NgoMIV G'CCGG_C
Cuts at: 0 720 2223
Size: 720 1503
NheI G'CTAG_C
Cuts at: 0 397 2223
Size: 397 1826
NspI r_CATG'y
Cuts at: 0 1317 1882 2223
Size: 1317 565 341
Fragments arranged by size:
1317 565 341
PasI CC'CwG_GG
Cuts at: 0 218 1010 1603 2223
Size: 218 792 593 620
Fragments arranged by size:
792 620 593 218
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 408 2223
Size: 252 156 1815
Fragments arranged by size:
1815 252 156
PfoI T'CCnGG_A
Cuts at: 0 2206 2223
Size: 2206 17
PmlI CAC'GTG
Cuts at: 0 2084 2223
Size: 2084 139
PpuMI rG'GwC_Cy
Cuts at: 0 826 1181 1764 2223
Size: 826 355 583 459
Fragments arranged by size:
826 583 459 355
PspOMI G'GGCC_C
Cuts at: 0 472 609 792 1886 1898 2223
Size: 472 137 183 1094 12 325
Fragments arranged by size:
1094 472 325 183 137 12
PstI C_TGCA'G
Cuts at: 0 1241 2223
Size: 1241 982
PvuII CAG'CTG
Cuts at: 0 270 928 979 2223
Size: 270 658 51 1244
Fragments arranged by size:
1244 658 270 51
SacI G_AGCT'C
Cuts at: 0 1967 2223
Size: 1967 256
SacII CC_GC'GG
Cuts at: 0 1100 2223
Size: 1100 1123
SfcI C'TryA_G
Cuts at: 0 36 1237 2223
Size: 36 1201 986
Fragments arranged by size:
1201 986 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 946 2223
Size: 946 1277
SfoI GGC'GCC
Cuts at: 0 2193 2223
Size: 2193 30
SmaI CCC'GGG
Cuts at: 0 1804 2223
Size: 1804 419
SmlI C'TyrA_G
Cuts at: 0 1194 2223
Size: 1194 1029
StuI AGG'CCT
Cuts at: 0 194 2223
Size: 194 2029
StyI C'CwwG_G
Cuts at: 0 935 1476 1487 2223
Size: 935 541 11 736
Fragments arranged by size:
935 736 541 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 888 1122 1631 2223
Size: 888 234 509 592
Fragments arranged by size:
888 592 509 234
TatI w'GTAC_w
Cuts at: 0 2045 2223
Size: 2045 178
Tth111I GACn'n_nGTC
Cuts at: 0 70 2223
Size: 70 2153
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 826 942 2223
Size: 826 116 1281
Fragments arranged by size:
1281 826 116
XmaI C'CCGG_G
Cuts at: 0 1802 2223
Size: 1802 421
Enzymes that do cut:
AarI AcuI AfeI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PasI PflMI PfoI PmlI PpuMI PspOMI PstI
PvuII SacI SacII SfcI SfiI SfoI SmaI SmlI
StuI StyI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BclI BfrBI BglII BmgBI BsaXI BsiWI BsmI
BsrBI BsrGI BstBI BstEII BstZ17I Bsu36I ClaI DraI
EciI EcoRV FalI FseI FspI FspAI HincII HpaI
KpnI MfeI MluI NcoI NdeI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TstI XbaI XhoI XmnI ZraI