Restriction map for
pF1KA1861
(Linear) (Six-base) MAPSORT of: KIAA1861.seq Check: 6243 from: 1 to: 2871
KIAA1861.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AccI GT'mk_AC
Cuts at: 0 1989 2532 2871
Size: 1989 543 339
Fragments arranged by size:
1989 543 339
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1028 1073 1097 1301 2200 2248 2871
Size: 1028 45 24 204 899 48 623
Fragments arranged by size:
1028 899 623 204 48 45 24
AflII C'TTAA_G
Cuts at: 0 1861 2871
Size: 1861 1010
AflIII A'CryG_T
Cuts at: 0 921 2871
Size: 921 1950
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 978 1261 2515 2871
Size: 978 283 1254 356
Fragments arranged by size:
1254 978 356 283
AlwNI CAG_nnn'CTG
Cuts at: 0 15 477 1051 2361 2871
Size: 15 462 574 1310 510
Fragments arranged by size:
1310 574 510 462 15
ApoI r'AATT_y
Cuts at: 0 85 645 717 886 1261 1407 1432 1438
Size: 85 560 72 169 375 146 25 6
Cuts at: 1438 1553 2443 2680 2871
Size: 115 890 237 191
Fragments arranged by size:
890 560 375 237 191 169 146 115
85 72 25 6
AseI AT'TA_AT
Cuts at: 0 140 2871
Size: 140 2731
AvaI C'yCGr_G
Cuts at: 0 1733 2871
Size: 1733 1138
BamHI G'GATC_C
Cuts at: 0 2800 2871
Size: 2800 71
BanI G'GyrC_C
Cuts at: 0 49 2094 2871
Size: 49 2045 777
Fragments arranged by size:
2045 777 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 355 405 2871
Size: 355 50 2466
Fragments arranged by size:
2466 355 50
BciVI GTATCCnnnnn_n'
Cuts at: 0 1029 2133 2871
Size: 1029 1104 738
Fragments arranged by size:
1104 1029 738
BlpI GC'TnA_GC
Cuts at: 0 988 2871
Size: 988 1883
Bme1580I G_kGCm'C
Cuts at: 0 2604 2871
Size: 2604 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 571 2871
Size: 220 351 2300
Fragments arranged by size:
2300 351 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 104 416 2871
Size: 11 93 312 2455
Fragments arranged by size:
2455 312 93 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 568 2871
Size: 568 2303
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 2871
Size: 887 1984
BsiHKAI G_wGCw'C
Cuts at: 0 2750 2871
Size: 2750 121
BsmI GAATG_Cn'
Cuts at: 0 1791 2871
Size: 1791 1080
Bsp1286I G_dGCh'C
Cuts at: 0 2604 2750 2871
Size: 2604 146 121
Fragments arranged by size:
2604 146 121
BspHI T'CATG_A
Cuts at: 0 1428 2871
Size: 1428 1443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1229 2871
Size: 1229 1642
BsrDI GCAATG_nn'
Cuts at: 0 349 514 791 2871
Size: 349 165 277 2080
Fragments arranged by size:
2080 349 277 165
BsrGI T'GTAC_A
Cuts at: 0 385 916 2871
Size: 385 531 1955
Fragments arranged by size:
1955 531 385
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2321 2871
Size: 2321 550
BstYI r'GATC_y
Cuts at: 0 909 2800 2871
Size: 909 1891 71
Fragments arranged by size:
1891 909 71
BstZ17I GTA'TAC
Cuts at: 0 2533 2871
Size: 2533 338
Bsu36I CC'TnA_GG
Cuts at: 0 1670 2871
Size: 1670 1201
BtgI C'CryG_G
Cuts at: 0 2174 2871
Size: 2174 697
DraI TTT'AAA
Cuts at: 0 618 1437 2871
Size: 618 819 1434
Fragments arranged by size:
1434 819 618
DraIII CAC_nnn'GTG
Cuts at: 0 2121 2871
Size: 2121 750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 571 1028 1073 1097 1301 2200 2248
Size: 220 351 457 45 24 204 899 48
Cuts at: 2248 2871
Size: 623
Fragments arranged by size:
899 623 457 351 220 204 48 45
24
EcoO109I rG'GnC_Cy
Cuts at: 0 69 2871
Size: 69 2802
EcoRI G'AATT_C
Cuts at: 0 85 645 2871
Size: 85 560 2226
Fragments arranged by size:
2226 560 85
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 862 2041 2871
Size: 862 1179 830
Fragments arranged by size:
1179 862 830
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1623 1655 2792 2824 2871
Size: 1623 32 1137 32 47
Fragments arranged by size:
1623 1137 47 32 32
HindIII A'AGCT_T
Cuts at: 0 205 752 2702 2871
Size: 205 547 1950 169
Fragments arranged by size:
1950 547 205 169
MfeI C'AATT_G
Cuts at: 0 1868 2611 2871
Size: 1868 743 260
Fragments arranged by size:
1868 743 260
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1233 1812 1924 2871
Size: 1233 579 112 947
Fragments arranged by size:
1233 947 579 112
MslI CAynn'nnrTG
Cuts at: 0 1028 1137 1571 2228 2871
Size: 1028 109 434 657 643
Fragments arranged by size:
1028 657 643 434 109
MspA1I CmG'CkG
Cuts at: 0 687 2047 2772 2871
Size: 687 1360 725 99
Fragments arranged by size:
1360 725 687 99
NdeI CA'TA_TG
Cuts at: 0 800 2871
Size: 800 2071
NspI r_CATG'y
Cuts at: 0 925 2871
Size: 925 1946
PciI A'CATG_T
Cuts at: 0 921 2871
Size: 921 1950
PfoI T'CCnGG_A
Cuts at: 0 567 2871
Size: 567 2304
PpuMI rG'GwC_Cy
Cuts at: 0 69 2871
Size: 69 2802
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2516 2548 2871
Size: 2516 32 323
Fragments arranged by size:
2516 323 32
PstI C_TGCA'G
Cuts at: 0 276 2247 2871
Size: 276 1971 624
Fragments arranged by size:
1971 624 276
PvuII CAG'CTG
Cuts at: 0 687 2047 2772 2871
Size: 687 1360 725 99
Fragments arranged by size:
1360 725 687 99
SanDI GG'GwC_CC
Cuts at: 0 69 2871
Size: 69 2802
ScaI AGT'ACT
Cuts at: 0 1528 2871
Size: 1528 1343
SfcI C'TryA_G
Cuts at: 0 272 1530 2243 2843 2871
Size: 272 1258 713 600 28
Fragments arranged by size:
1258 713 600 272 28
SmlI C'TyrA_G
Cuts at: 0 26 119 431 1861 2871
Size: 26 93 312 1430 1010
Fragments arranged by size:
1430 1010 312 93 26
SspI AAT'ATT
Cuts at: 0 411 2758 2871
Size: 411 2347 113
Fragments arranged by size:
2347 411 113
StuI AGG'CCT
Cuts at: 0 446 2871
Size: 446 2425
StyI C'CwwG_G
Cuts at: 0 740 2183 2871
Size: 740 1443 688
Fragments arranged by size:
1443 740 688
SwaI ATTT'AAAT
Cuts at: 0 1437 2871
Size: 1437 1434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 826 2871
Size: 826 2045
TatI w'GTAC_w
Cuts at: 0 385 916 1526 2871
Size: 385 531 610 1345
Fragments arranged by size:
1345 610 531 385
Tth111I GACn'n_nGTC
Cuts at: 0 11 2871
Size: 11 2860
XbaI T'CTAG_A
Cuts at: 0 2034 2871
Size: 2034 837
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2181 2871
Size: 2181 690
Enzymes that do cut:
AccI AcuI AflII AflIII AlfI AlwNI ApoI AseI
AvaI BamHI BanI BbsI BciVI BlpI Bme1580I BpmI
BpuEI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrGI BstXI BstYI BstZ17I Bsu36I BtgI DraI
DraIII Eco57MI EcoO109I EcoRI FalI Hin4I HindIII MfeI
MmeI MslI MspA1I NdeI NspI PciI PfoI PpuMI
PsrI PstI PvuII SanDI ScaI SfcI SmlI SspI
StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BanII
BbeI BbvCI BcgI BclI BfrBI BglI BglII BmgBI
BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BtgZI BtsI
ClaI CspCI DrdI EaeI EagI EarI EciI EcoICRI
EcoNI EcoRV FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI TstI
XhoI XmaI XmnI ZraI