Restriction map for
pF1KSDA1862
(Linear) (Six-base) MAPSORT of: KIAA1862.seq Check: 2802 from: 1 to: 3090
KIAA1862.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 847 1192 1926 1973 3090
Size: 664 183 345 734 47 1117
Fragments arranged by size:
1117 734 664 345 183 47
AfeI AGC'GCT
Cuts at: 0 2459 3090
Size: 2459 631
AhdI GACnn_n'nnGTC
Cuts at: 0 1254 3090
Size: 1254 1836
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 146 3090
Size: 146 2944
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 903 935 3090
Size: 903 32 2155
Fragments arranged by size:
2155 903 32
AlwNI CAG_nnn'CTG
Cuts at: 0 82 813 1030 1075 1506 1607 1978 2126
Size: 82 731 217 45 431 101 371 148
Cuts at: 2126 2406 2799 2802 3090
Size: 280 393 3 288
Fragments arranged by size:
731 431 393 371 288 280 217 148
101 82 45 3
ApaI G_GGCC'C
Cuts at: 0 668 1114 2284 3036 3090
Size: 668 446 1170 752 54
Fragments arranged by size:
1170 752 668 446 54
ApoI r'AATT_y
Cuts at: 0 599 3090
Size: 599 2491
AvaI C'yCGr_G
Cuts at: 0 256 304 676 838 1376 1660 1935 1965
Size: 256 48 372 162 538 284 275 30
Cuts at: 1965 3072 3090
Size: 1107 18
Fragments arranged by size:
1107 538 372 284 275 256 162 48
30 18
BamHI G'GATC_C
Cuts at: 0 2157 2981 3090
Size: 2157 824 109
Fragments arranged by size:
2157 824 109
BanI G'GyrC_C
Cuts at: 0 671 2519 2970 3090
Size: 671 1848 451 120
Fragments arranged by size:
1848 671 451 120
BanII G_rGCy'C
Cuts at: 0 304 329 513 527 668 760 836 1021
Size: 304 25 184 14 141 92 76 185
Cuts at: 1021 1114 1601 1985 2120 2284 2592 2777 2878
Size: 93 487 384 135 164 308 185 101
Cuts at: 2878 3036 3090
Size: 158 54
Fragments arranged by size:
487 384 308 304 185 185 184 164
158 141 135 101 93 92 76 54
25 14
BbeI G_GCGC'C
Cuts at: 0 2523 3090
Size: 2523 567
BbsI GAAGACnn'nnnn_
Cuts at: 0 781 1967 2105 2241 3090
Size: 781 1186 138 136 849
Fragments arranged by size:
1186 849 781 138 136
BbvCI CC'TCA_GC
Cuts at: 0 1068 1566 1637 3090
Size: 1068 498 71 1453
Fragments arranged by size:
1453 1068 498 71
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 329 2118 2152 2994 3028 3090
Size: 295 34 1789 34 842 34 62
Fragments arranged by size:
1789 842 295 62 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 928 3090
Size: 928 2162
BglI GCCn_nnn'nGGC
Cuts at: 0 1050 1554 1875 2528 3090
Size: 1050 504 321 653 562
Fragments arranged by size:
1050 653 562 504 321
BlpI GC'TnA_GC
Cuts at: 0 41 349 3090
Size: 41 308 2741
Fragments arranged by size:
2741 308 41
Bme1580I G_kGCm'C
Cuts at: 0 668 721 1114 2284 2733 3036 3090
Size: 668 53 393 1170 449 303 54
Fragments arranged by size:
1170 668 449 393 303 54 53
BmgBI CAC'GTC
Cuts at: 0 722 2734 2952 3090
Size: 722 2012 218 138
Fragments arranged by size:
2012 722 218 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 854 1428 2291 3090
Size: 854 574 863 799
Fragments arranged by size:
863 854 799 574
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 364 3090
Size: 364 2726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 944 1097 1528 1710 1864 1914 2200
Size: 790 154 153 431 182 154 50 286
Cuts at: 2200 2239 2643 2694 2729 3022 3090
Size: 39 404 51 35 293 68
Fragments arranged by size:
790 431 404 293 286 182 154 154
153 68 51 50 39 35
Bpu10I CC'TnA_GC
Cuts at: 0 1068 1566 1637 1979 2153 2288 2474 3090
Size: 1068 498 71 342 174 135 186 616
Fragments arranged by size:
1068 616 498 342 186 174 135 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1106 1122 1504 3090
Size: 1106 16 382 1586
Fragments arranged by size:
1586 1106 382 16
BsaI GGTCTCn'nnnn_
Cuts at: 0 30 1262 3090
Size: 30 1232 1828
Fragments arranged by size:
1828 1232 30
BsaHI Gr'CG_yC
Cuts at: 0 2520 3090
Size: 2520 570
BsaWI w'CCGG_w
Cuts at: 0 2313 2328 3090
Size: 2313 15 762
Fragments arranged by size:
2313 762 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 936 966 2831 2861 3090
Size: 936 30 1865 30 229
Fragments arranged by size:
1865 936 229 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 941 1389 1554 1602 2349 2839 2915 3035
Size: 941 448 165 48 747 490 76 120
Cuts at: 3035 3090
Size: 55
Fragments arranged by size:
941 747 490 448 165 120 76 55
48
BseYI C'CCAG_C
Cuts at: 0 526 547 1007 1102 1106 1205 1238 1357
Size: 526 21 460 95 4 99 33 119
Cuts at: 1357 1906 1912 1918 2020 2511 2585 2792 2815
Size: 549 6 6 102 491 74 207 23
Cuts at: 2815 3015 3090
Size: 200 75
Fragments arranged by size:
549 526 491 460 207 200 119 102
99 95 75 74 33 23 21 6
6 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 214 1819 2162 2392 2822 2960 3046
Size: 160 54 1605 343 230 430 138 86
Cuts at: 3046 3090
Size: 44
Fragments arranged by size:
1605 430 343 230 160 138 86 54
44
BsiHKAI G_wGCw'C
Cuts at: 0 836 1985 3065 3090
Size: 836 1149 1080 25
Fragments arranged by size:
1149 1080 836 25
BsmI GAATG_Cn'
Cuts at: 0 1178 3090
Size: 1178 1912
BsmBI CGTCTCn'nnnn_
Cuts at: 0 10 1663 3090
Size: 10 1653 1427
Fragments arranged by size:
1653 1427 10
Bsp1286I G_dGCh'C
Cuts at: 0 304 329 513 527 668 721 760 836
Size: 304 25 184 14 141 53 39 76
Cuts at: 836 1021 1114 1601 1985 2120 2284 2592 2733
Size: 185 93 487 384 135 164 308 141
Cuts at: 2733 2777 2878 3036 3065 3090
Size: 44 101 158 29 25
Fragments arranged by size:
487 384 308 304 185 184 164 158
141 141 135 101 93 76 53 44
39 29 25 25 14
BspEI T'CCGG_A
Cuts at: 0 2313 2328 3090
Size: 2313 15 762
Fragments arranged by size:
2313 762 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1038 2134 2705 3090
Size: 1038 1096 571 385
Fragments arranged by size:
1096 1038 571 385
BsrBI CCG'CTC
Cuts at: 0 1995 2722 2991 3090
Size: 1995 727 269 99
Fragments arranged by size:
1995 727 269 99
BssSI C'ACGA_G
Cuts at: 0 411 2640 3090
Size: 411 2229 450
Fragments arranged by size:
2229 450 411
BstAPI GCAn_nnn'nTGC
Cuts at: 0 174 2406 2938 3090
Size: 174 2232 532 152
Fragments arranged by size:
2232 532 174 152
BstEII G'GTnAC_C
Cuts at: 0 2692 3090
Size: 2692 398
BstXI CCAn_nnnn'nTGG
Cuts at: 0 908 1790 2217 2460 2533 3090
Size: 908 882 427 243 73 557
Fragments arranged by size:
908 882 557 427 243 73
BstYI r'GATC_y
Cuts at: 0 144 209 1862 2157 2657 2981 3090
Size: 144 65 1653 295 500 324 109
Fragments arranged by size:
1653 500 324 295 144 109 65
Bsu36I CC'TnA_GG
Cuts at: 0 867 1115 1855 2961 3090
Size: 867 248 740 1106 129
Fragments arranged by size:
1106 867 740 248 129
BtgI C'CryG_G
Cuts at: 0 481 2572 3090
Size: 481 2091 518
Fragments arranged by size:
2091 518 481
BtsI GCAGTG_nn'
Cuts at: 0 776 1495 1836 2085 2778 3090
Size: 776 719 341 249 693 312
Fragments arranged by size:
776 719 693 341 312 249
DraIII CAC_nnn'GTG
Cuts at: 0 1294 3090
Size: 1294 1796
EaeI y'GGCC_r
Cuts at: 0 389 2483 2667 3090
Size: 389 2094 184 423
Fragments arranged by size:
2094 423 389 184
EarI CTCTTCn'nnn_
Cuts at: 0 81 553 2793 3090
Size: 81 472 2240 297
Fragments arranged by size:
2240 472 297 81
EcoICRI GAG'CTC
Cuts at: 0 834 1983 3090
Size: 834 1149 1107
Fragments arranged by size:
1149 1107 834
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 790 847 944 1097 1192 1528 1710
Size: 664 126 57 97 153 95 336 182
Cuts at: 1710 1864 1914 1926 1973 2200 2239 2643 2694
Size: 154 50 12 47 227 39 404 51
Cuts at: 2694 2729 3022 3090
Size: 35 293 68
Fragments arranged by size:
664 404 336 293 227 182 154 153
126 97 95 68 57 51 50 47
39 35 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 338 912 1469 2398 2473 2622 3090
Size: 338 574 557 929 75 149 468
Fragments arranged by size:
929 574 557 468 338 149 75
EcoO109I rG'GnC_Cy
Cuts at: 0 584 665 748 928 1027 1092 1110 1111
Size: 584 81 83 180 99 65 18 1
Cuts at: 1111 1364 1422 1877 1903 2063 2280 2495 2530
Size: 253 58 455 26 160 217 215 35
Cuts at: 2530 2578 2635 3032 3033 3069 3090
Size: 48 57 397 1 36 21
Fragments arranged by size:
584 455 397 253 217 215 180 160
99 83 81 65 58 57 48 36
35 26 21 18 1 1
HaeII r_GCGC'y
Cuts at: 0 245 1684 1853 2461 2523 3090
Size: 245 1439 169 608 62 567
Fragments arranged by size:
1439 608 567 245 169 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 936 968 1240 1272 1683 1715 3090
Size: 936 32 272 32 411 32 1375
Fragments arranged by size:
1375 936 411 272 32 32 32
HindIII A'AGCT_T
Cuts at: 0 690 2549 3090
Size: 690 1859 541
Fragments arranged by size:
1859 690 541
KasI G'GCGC_C
Cuts at: 0 2519 3090
Size: 2519 571
MscI TGG'CCA
Cuts at: 0 391 2485 2669 3090
Size: 391 2094 184 421
Fragments arranged by size:
2094 421 391 184
MslI CAynn'nnrTG
Cuts at: 0 113 3090
Size: 113 2977
MspA1I CmG'CkG
Cuts at: 0 40 198 313 353 1007 1106 1149 1209
Size: 40 158 115 40 654 99 43 60
Cuts at: 1209 1730 1752 1833 1946 2024 2132 2178 2739
Size: 521 22 81 113 78 108 46 561
Cuts at: 2739 2799 2819 3090
Size: 60 20 271
Fragments arranged by size:
654 561 521 271 158 115 113 108
99 81 78 60 60 46 43 40
40 22 20
NarI GG'CG_CC
Cuts at: 0 2520 3090
Size: 2520 570
PasI CC'CwG_GG
Cuts at: 0 150 513 764 1768 2192 2277 2533 3090
Size: 150 363 251 1004 424 85 256 557
Fragments arranged by size:
1004 557 424 363 256 251 150 85
PflMI CCAn_nnn'nTGG
Cuts at: 0 1607 2056 3090
Size: 1607 449 1034
Fragments arranged by size:
1607 1034 449
PfoI T'CCnGG_A
Cuts at: 0 900 1464 2554 3038 3090
Size: 900 564 1090 484 52
Fragments arranged by size:
1090 900 564 484 52
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1301 1333 3090
Size: 1301 32 1757
Fragments arranged by size:
1757 1301 32
PpuMI rG'GwC_Cy
Cuts at: 0 584 1027 1092 1422 2063 2495 2578 2635
Size: 584 443 65 330 641 432 83 57
Cuts at: 2635 3069 3090
Size: 434 21
Fragments arranged by size:
641 584 443 434 432 330 83 65
57 21
PshAI GACnn'nnGTC
Cuts at: 0 22 3090
Size: 22 3068
PspOMI G'GGCC_C
Cuts at: 0 664 1110 2280 3032 3090
Size: 664 446 1170 752 58
Fragments arranged by size:
1170 752 664 446 58
PspXI vC'TCGA_Gb
Cuts at: 0 1935 3090
Size: 1935 1155
PstI C_TGCA'G
Cuts at: 0 1337 1502 2131 2369 2399 2686 2785 2915
Size: 1337 165 629 238 30 287 99 130
Cuts at: 2915 2934 3090
Size: 19 156
Fragments arranged by size:
1337 629 287 238 165 156 130 99
30 19
PvuII CAG'CTG
Cuts at: 0 40 198 313 353 1007 1106 1209 1730
Size: 40 158 115 40 654 99 103 521
Cuts at: 1730 2024 2178 2799 2819 3090
Size: 294 154 621 20 271
Fragments arranged by size:
654 621 521 294 271 158 154 115
103 99 40 40 20
SacI G_AGCT'C
Cuts at: 0 836 1985 3090
Size: 836 1149 1105
Fragments arranged by size:
1149 1105 836
SanDI GG'GwC_CC
Cuts at: 0 1092 2063 2635 3069 3090
Size: 1092 971 572 434 21
Fragments arranged by size:
1092 971 572 434 21
SapI GCTCTTCn'nnn_
Cuts at: 0 81 553 2793 3090
Size: 81 472 2240 297
Fragments arranged by size:
2240 472 297 81
SbfI CC_TGCA'GG
Cuts at: 0 2915 3090
Size: 2915 175
SfcI C'TryA_G
Cuts at: 0 889 1333 1498 2127 2365 2395 2682 2781
Size: 889 444 165 629 238 30 287 99
Cuts at: 2781 2911 2930 3090
Size: 130 19 160
Fragments arranged by size:
889 629 444 287 238 165 160 130
99 30 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1554 3090
Size: 1554 1536
SfoI GGC'GCC
Cuts at: 0 2521 3090
Size: 2521 569
SmlI C'TyrA_G
Cuts at: 0 1121 1137 1519 1935 3090
Size: 1121 16 382 416 1155
Fragments arranged by size:
1155 1121 416 382 16
StuI AGG'CCT
Cuts at: 0 344 629 1642 2216 2468 2646 3090
Size: 344 285 1013 574 252 178 444
Fragments arranged by size:
1013 574 444 344 285 252 178
StyI C'CwwG_G
Cuts at: 0 2526 2996 3090
Size: 2526 470 94
Fragments arranged by size:
2526 470 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 553 793 1230 1599 2829 3090
Size: 553 240 437 369 1230 261
Fragments arranged by size:
1230 553 437 369 261 240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2831 2863 3034 3066 3090
Size: 2831 32 171 32 24
Fragments arranged by size:
2831 171 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 0 439 3090
Size: 439 2651
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 741 964 1365 3090
Size: 741 223 401 1725
Fragments arranged by size:
1725 741 401 223
XhoI C'TCGA_G
Cuts at: 0 1935 3090
Size: 1935 1155
Enzymes that do cut:
AcuI AfeI AhdI AlfI AloI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HindIII KasI MscI MslI MspA1I NarI PasI
PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SanDI SapI SbfI SfcI SfiI SfoI
SmlI StuI StyI TaqII TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI ApaLI AscI AseI AsiSI AvrII BaeI BclI
BfrBI BglII BmtI BsaAI BsaBI BsiEI BsiWI BspHI
BsrDI BsrFI BsrGI BssHII BstBI BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PsiI PsrI
PvuI RsrII SacII SalI ScaI SexAI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
XbaI XmaI XmnI ZraI