Restriction map for
pF1KA1867
(Linear) (Six-base) MAPSORT of: KIAA1867.seq Check: 3855 from: 1 to: 2334
KIAA1867.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 514 2334
Size: 514 1820
AatII G_ACGT'C
Cuts at: 0 721 992 2334
Size: 721 271 1342
Fragments arranged by size:
1342 721 271
AccI GT'mk_AC
Cuts at: 0 992 2301 2334
Size: 992 1309 33
Fragments arranged by size:
1309 992 33
AclI AA'CG_TT
Cuts at: 0 1373 2334
Size: 1373 961
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 132 355 1044 1909 2334
Size: 30 102 223 689 865 425
Fragments arranged by size:
865 689 425 223 102 30
AflIII A'CryG_T
Cuts at: 0 914 1187 2334
Size: 914 273 1147
Fragments arranged by size:
1147 914 273
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 380 1480 2334
Size: 380 1100 854
Fragments arranged by size:
1100 854 380
AlwNI CAG_nnn'CTG
Cuts at: 0 297 1591 1762 1887 2334
Size: 297 1294 171 125 447
Fragments arranged by size:
1294 447 297 171 125
ApaI G_GGCC'C
Cuts at: 0 466 1004 2334
Size: 466 538 1330
Fragments arranged by size:
1330 538 466
ApoI r'AATT_y
Cuts at: 0 1819 2334
Size: 1819 515
BanI G'GyrC_C
Cuts at: 0 432 1203 2334
Size: 432 771 1131
Fragments arranged by size:
1131 771 432
BanII G_rGCy'C
Cuts at: 0 466 932 1004 1048 1117 2334
Size: 466 466 72 44 69 1217
Fragments arranged by size:
1217 466 466 72 69 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 1147 2334
Size: 1147 1187
BbvCI CC'TCA_GC
Cuts at: 0 973 2146 2334
Size: 973 1173 188
Fragments arranged by size:
1173 973 188
BclI T'GATC_A
Cuts at: 0 470 2334
Size: 470 1864
BglI GCCn_nnn'nGGC
Cuts at: 0 391 2242 2334
Size: 391 1851 92
Fragments arranged by size:
1851 391 92
Bme1580I G_kGCm'C
Cuts at: 0 466 1004 1208 1390 1683 2009 2334
Size: 466 538 204 182 293 326 325
Fragments arranged by size:
538 466 326 325 293 204 182
BmgBI CAC'GTC
Cuts at: 0 654 2331 2334
Size: 654 1677 3
Fragments arranged by size:
1677 654 3
BmrI ACTGGGnnnn_n'
Cuts at: 0 834 2265 2291 2334
Size: 834 1431 26 43
Fragments arranged by size:
1431 834 43 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 951 1800 1956 2161 2334
Size: 951 849 156 205 173
Fragments arranged by size:
951 849 205 173 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 805 905 1251 1399 1870 1947 2334
Size: 49 756 100 346 148 471 77 387
Fragments arranged by size:
756 471 387 346 148 100 77 49
Bpu10I CC'TnA_GC
Cuts at: 0 973 1129 2044 2146 2334
Size: 973 156 915 102 188
Fragments arranged by size:
973 915 188 156 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 1525 2334
Size: 264 1261 809
Fragments arranged by size:
1261 809 264
BsaI GGTCTCn'nnnn_
Cuts at: 0 1405 2334
Size: 1405 929
BsaAI yAC'GTr
Cuts at: 0 917 1188 2334
Size: 917 271 1146
Fragments arranged by size:
1146 917 271
BsaHI Gr'CG_yC
Cuts at: 0 718 989 1433 2334
Size: 718 271 444 901
Fragments arranged by size:
901 718 444 271
BsaWI w'CCGG_w
Cuts at: 0 1117 1325 2334
Size: 1117 208 1009
Fragments arranged by size:
1117 1009 208
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1619 1649 1861 1891 2143 2173 2334
Size: 1619 30 212 30 252 30 161
Fragments arranged by size:
1619 252 212 161 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1441 1792 1882 2247 2334
Size: 1441 351 90 365 87
Fragments arranged by size:
1441 365 351 90 87
BseYI C'CCAG_C
Cuts at: 0 57 90 828 1280 2088 2334
Size: 57 33 738 452 808 246
Fragments arranged by size:
808 738 452 246 57 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1479 2242 2334
Size: 1479 763 92
Fragments arranged by size:
1479 763 92
BsiHKAI G_wGCw'C
Cuts at: 0 329 1333 1784 1937 2334
Size: 329 1004 451 153 397
Fragments arranged by size:
1004 451 397 329 153
BsmBI CGTCTCn'nnnn_
Cuts at: 0 709 939 2334
Size: 709 230 1395
Fragments arranged by size:
1395 709 230
Bsp1286I G_dGCh'C
Cuts at: 0 329 466 932 1004 1048 1117 1208 1333
Size: 329 137 466 72 44 69 91 125
Cuts at: 1333 1390 1683 1784 1937 2009 2334
Size: 57 293 101 153 72 325
Fragments arranged by size:
466 329 325 293 153 137 125 101
91 72 72 69 57 44
BspEI T'CCGG_A
Cuts at: 0 1117 1325 2334
Size: 1117 208 1009
Fragments arranged by size:
1117 1009 208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 514 1988 2334
Size: 514 1474 346
Fragments arranged by size:
1474 514 346
BsrBI CCG'CTC
Cuts at: 0 404 2048 2124 2334
Size: 404 1644 76 210
Fragments arranged by size:
1644 404 210 76
BsrDI GCAATG_nn'
Cuts at: 0 1140 2334
Size: 1140 1194
BsrFI r'CCGG_y
Cuts at: 0 1075 2334
Size: 1075 1259
BsrGI T'GTAC_A
Cuts at: 0 893 2334
Size: 893 1441
BssSI C'ACGA_G
Cuts at: 0 1033 2334
Size: 1033 1301
BstAPI GCAn_nnn'nTGC
Cuts at: 0 520 2004 2334
Size: 520 1484 330
Fragments arranged by size:
1484 520 330
BstEII G'GTnAC_C
Cuts at: 0 723 945 1233 2334
Size: 723 222 288 1101
Fragments arranged by size:
1101 723 288 222
BstXI CCAn_nnnn'nTGG
Cuts at: 0 957 2334
Size: 957 1377
BstYI r'GATC_y
Cuts at: 0 338 1038 2334
Size: 338 700 1296
Fragments arranged by size:
1296 700 338
Bsu36I CC'TnA_GG
Cuts at: 0 343 2334
Size: 343 1991
BtgI C'CryG_G
Cuts at: 0 1293 2334
Size: 1293 1041
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1335 2334
Size: 1335 999
BtsI GCAGTG_nn'
Cuts at: 0 807 2182 2334
Size: 807 1375 152
Fragments arranged by size:
1375 807 152
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1646 1681 2334
Size: 1646 35 653
Fragments arranged by size:
1646 653 35
DraIII CAC_nnn'GTG
Cuts at: 0 2177 2334
Size: 2177 157
DrdI GACnn_nn'nnGTC
Cuts at: 0 796 2188 2334
Size: 796 1392 146
Fragments arranged by size:
1392 796 146
EaeI y'GGCC_r
Cuts at: 0 101 663 1601 1619 2049 2334
Size: 101 562 938 18 430 285
Fragments arranged by size:
938 562 430 285 101 18
EarI CTCTTCn'nnn_
Cuts at: 0 31 49 64 640 1859 2334
Size: 31 18 15 576 1219 475
Fragments arranged by size:
1219 576 475 31 18 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2331 2334
Size: 2331 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 49 132 355 805 905 1044 1251
Size: 30 19 83 223 450 100 139 207
Cuts at: 1251 1399 1870 1909 1947 2334
Size: 148 471 39 38 387
Fragments arranged by size:
471 450 387 223 207 148 139 100
83 39 38 30 19
EcoO109I rG'GnC_Cy
Cuts at: 0 273 462 463 489 1001 1854 1893 2334
Size: 273 189 1 26 512 853 39 441
Fragments arranged by size:
853 512 441 273 189 39 26 1
EcoRI G'AATT_C
Cuts at: 0 1819 2334
Size: 1819 515
EcoRV GAT'ATC
Cuts at: 0 1728 2334
Size: 1728 606
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 267 2334
Size: 267 2067
HaeII r_GCGC'y
Cuts at: 0 1401 2334
Size: 1401 933
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 551 583 1030 1062 1244 1276 2143 2175
Size: 551 32 447 32 182 32 867 32
Cuts at: 2175 2310 2334
Size: 135 24
Fragments arranged by size:
867 551 447 182 135 32 32 32
32 24
HincII GTy'rAC
Cuts at: 0 756 987 1920 2302 2334
Size: 756 231 933 382 32
Fragments arranged by size:
933 756 382 231 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1544 2334
Size: 1544 790
MscI TGG'CCA
Cuts at: 0 103 665 2334
Size: 103 562 1669
Fragments arranged by size:
1669 562 103
MslI CAynn'nnrTG
Cuts at: 0 1615 2014 2334
Size: 1615 399 320
Fragments arranged by size:
1615 399 320
MspA1I CmG'CkG
Cuts at: 0 832 1064 1800 1848 1967 2079 2201 2313
Size: 832 232 736 48 119 112 122 112
Cuts at: 2313 2334
Size: 21
Fragments arranged by size:
832 736 232 122 119 112 112 48
21
NspI r_CATG'y
Cuts at: 0 2019 2334
Size: 2019 315
PasI CC'CwG_GG
Cuts at: 0 957 2334
Size: 957 1377
PflMI CCAn_nnn'nTGG
Cuts at: 0 174 545 1100 1762 2334
Size: 174 371 555 662 572
Fragments arranged by size:
662 572 555 371 174
PfoI T'CCnGG_A
Cuts at: 0 1360 2165 2334
Size: 1360 805 169
Fragments arranged by size:
1360 805 169
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2113 2145 2334
Size: 2113 32 189
Fragments arranged by size:
2113 189 32
PpuMI rG'GwC_Cy
Cuts at: 0 273 489 1854 1893 2334
Size: 273 216 1365 39 441
Fragments arranged by size:
1365 441 273 216 39
PshAI GACnn'nnGTC
Cuts at: 0 721 1096 1982 2297 2329 2334
Size: 721 375 886 315 32 5
Fragments arranged by size:
886 721 375 315 32 5
PspOMI G'GGCC_C
Cuts at: 0 462 1000 2334
Size: 462 538 1334
Fragments arranged by size:
1334 538 462
PstI C_TGCA'G
Cuts at: 0 534 2312 2334
Size: 534 1778 22
Fragments arranged by size:
1778 534 22
PvuII CAG'CTG
Cuts at: 0 832 1064 1800 1848 1967 2334
Size: 832 232 736 48 119 367
Fragments arranged by size:
832 736 367 232 119 48
RsrII CG'GwC_CG
Cuts at: 0 1344 2334
Size: 1344 990
SalI G'TCGA_C
Cuts at: 0 2300 2334
Size: 2300 34
SanDI GG'GwC_CC
Cuts at: 0 489 2334
Size: 489 1845
SapI GCTCTTCn'nnn_
Cuts at: 0 31 2334
Size: 31 2303
SexAI A'CCwGG_T
Cuts at: 0 167 296 2334
Size: 167 129 2038
Fragments arranged by size:
2038 167 129
SfcI C'TryA_G
Cuts at: 0 530 1911 2034 2308 2334
Size: 530 1381 123 274 26
Fragments arranged by size:
1381 530 274 123 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 391 2334
Size: 391 1943
SmlI C'TyrA_G
Cuts at: 0 279 1540 2334
Size: 279 1261 794
Fragments arranged by size:
1261 794 279
StyI C'CwwG_G
Cuts at: 0 104 2334
Size: 104 2230
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 250 711 853 1288 2334
Size: 250 461 142 435 1046
Fragments arranged by size:
1046 461 435 250 142
TatI w'GTAC_w
Cuts at: 0 842 893 2334
Size: 842 51 1441
Fragments arranged by size:
1441 842 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 176 208 1619 1651 2161 2193 2257 2289
Size: 176 32 1411 32 510 32 64 32
Cuts at: 2289 2334
Size: 45
Fragments arranged by size:
1411 510 176 64 45 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 448 793 1834 2334
Size: 448 345 1041 500
Fragments arranged by size:
1041 500 448 345
XmnI GAAnn'nnTTC
Cuts at: 0 7 1510 2334
Size: 7 1503 824
Fragments arranged by size:
1503 824 7
ZraI GAC'GTC
Cuts at: 0 719 990 2334
Size: 719 271 1344
Fragments arranged by size:
1344 719 271
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflIII AlfI AlwNI
ApaI ApoI BanI BanII BbsI BbvCI BclI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NspI PasI PflMI PfoI PpiI
PpuMI PshAI PspOMI PstI PvuII RsrII SalI SanDI
SapI SexAI SfcI SfiI SmlI StyI TaqII TatI
TstI Tth111I XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AgeI AhdI AleI AloI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BcgI BciVI BfrBI BglII BlpI BmtI BsaBI BsiEI
BsiWI BsmI BspHI BssHII BstBI BstZ17I ClaI DraI
EagI EcoICRI EcoNI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PsiI PspXI PsrI PvuI SacI SacII SbfI
ScaI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XcmI XhoI XmaI