Restriction map for
pF1KSDA1869
(Linear) (Six-base) MAPSORT of: KIAA1869.seq Check: 8402 from: 1 to: 2790
KIAA1869.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2468 2790
Size: 2468 322
AatII G_ACGT'C
Cuts at: 0 1905 2705 2790
Size: 1905 800 85
Fragments arranged by size:
1905 800 85
AccI GT'mk_AC
Cuts at: 0 707 1535 2790
Size: 707 828 1255
Fragments arranged by size:
1255 828 707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1372 2746 2790
Size: 862 510 1374 44
Fragments arranged by size:
1374 862 510 44
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1672 1704 2790
Size: 1672 32 1086
Fragments arranged by size:
1672 1086 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1631 1861 2790
Size: 1631 230 929
Fragments arranged by size:
1631 929 230
ApaI G_GGCC'C
Cuts at: 0 620 1263 1984 2231 2577 2747 2790
Size: 620 643 721 247 346 170 43
Fragments arranged by size:
721 643 620 346 247 170 43
AvaI C'yCGr_G
Cuts at: 0 230 776 809 1154 2101 2272 2470 2594
Size: 230 546 33 345 947 171 198 124
Cuts at: 2594 2604 2635 2665 2790
Size: 10 31 30 125
Fragments arranged by size:
947 546 345 230 198 171 125 124
33 31 30 10
BamHI G'GATC_C
Cuts at: 0 1111 2790
Size: 1111 1679
BanI G'GyrC_C
Cuts at: 0 285 1849 2790
Size: 285 1564 941
Fragments arranged by size:
1564 941 285
BanII G_rGCy'C
Cuts at: 0 620 1263 1394 1934 1984 1991 2231 2357
Size: 620 643 131 540 50 7 240 126
Cuts at: 2357 2396 2577 2747 2790
Size: 39 181 170 43
Fragments arranged by size:
643 620 540 240 181 170 131 126
50 43 39 7
BbeI G_GCGC'C
Cuts at: 0 1853 2790
Size: 1853 937
BbsI GAAGACnn'nnnn_
Cuts at: 0 945 2790
Size: 945 1845
BbvCI CC'TCA_GC
Cuts at: 0 2452 2790
Size: 2452 338
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1899 1933 2790
Size: 1899 34 857
Fragments arranged by size:
1899 857 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1828 2454 2790
Size: 1828 626 336
Fragments arranged by size:
1828 626 336
Bme1580I G_kGCm'C
Cuts at: 0 12 290 620 1263 1560 1984 2072 2216
Size: 12 278 330 643 297 424 88 144
Cuts at: 2216 2231 2468 2577 2747 2790
Size: 15 237 109 170 43
Fragments arranged by size:
643 424 330 297 278 237 170 144
109 88 43 15 12
BmgBI CAC'GTC
Cuts at: 0 2463 2790
Size: 2463 327
BmrI ACTGGGnnnn_n'
Cuts at: 0 516 920 1733 2709 2790
Size: 516 404 813 976 81
Fragments arranged by size:
976 813 516 404 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2460 2790
Size: 2460 330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1501 1608 2320 2601 2790
Size: 169 1332 107 712 281 189
Fragments arranged by size:
1332 712 281 189 169 107
Bpu10I CC'TnA_GC
Cuts at: 0 1388 2452 2790
Size: 1388 1064 338
Fragments arranged by size:
1388 1064 338
BsaI GGTCTCn'nnnn_
Cuts at: 0 692 732 1151 1878 2790
Size: 692 40 419 727 912
Fragments arranged by size:
912 727 692 419 40
BsaBI GATnn'nnATC
Cuts at: 0 1667 2790
Size: 1667 1123
BsaHI Gr'CG_yC
Cuts at: 0 1850 1902 2026 2534 2702 2790
Size: 1850 52 124 508 168 88
Fragments arranged by size:
1850 508 168 124 88 52
BsaWI w'CCGG_w
Cuts at: 0 209 2058 2790
Size: 209 1849 732
Fragments arranged by size:
1849 732 209
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 229 259 689 719 2790
Size: 229 30 430 30 2071
Fragments arranged by size:
2071 430 229 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1065 1137 1593 1804 2739 2790
Size: 1065 72 456 211 935 51
Fragments arranged by size:
1065 935 456 211 72 51
BseYI C'CCAG_C
Cuts at: 0 620 747 1675 1779 2419 2529 2790
Size: 620 127 928 104 640 110 261
Fragments arranged by size:
928 640 620 261 127 110 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1139 1455 2041 2305 2790
Size: 778 361 316 586 264 485
Fragments arranged by size:
778 586 485 361 316 264
BsiEI CG_ry'CG
Cuts at: 0 2778 2790
Size: 2778 12
Bsp1286I G_dGCh'C
Cuts at: 0 12 290 620 1263 1394 1560 1934 1984
Size: 12 278 330 643 131 166 374 50
Cuts at: 1984 1991 2072 2216 2231 2357 2396 2468 2577
Size: 7 81 144 15 126 39 72 109
Cuts at: 2577 2747 2790
Size: 170 43
Fragments arranged by size:
643 374 330 278 170 166 144 131
126 109 81 72 50 43 39 15
12 7
BspEI T'CCGG_A
Cuts at: 0 2058 2790
Size: 2058 732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 932 1781 2327 2468 2790
Size: 932 849 546 141 322
Fragments arranged by size:
932 849 546 322 141
BsrBI CCG'CTC
Cuts at: 0 635 935 1796 2201 2521 2790
Size: 635 300 861 405 320 269
Fragments arranged by size:
861 635 405 320 300 269
BsrFI r'CCGG_y
Cuts at: 0 1550 2121 2660 2790
Size: 1550 571 539 130
Fragments arranged by size:
1550 571 539 130
BssSI C'ACGA_G
Cuts at: 0 2434 2790
Size: 2434 356
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1840 2790
Size: 1840 950
BstXI CCAn_nnnn'nTGG
Cuts at: 0 134 1401 2790
Size: 134 1267 1389
Fragments arranged by size:
1389 1267 134
BstYI r'GATC_y
Cuts at: 0 1111 1648 2790
Size: 1111 537 1142
Fragments arranged by size:
1142 1111 537
BtgI C'CryG_G
Cuts at: 0 1691 1971 2282 2790
Size: 1691 280 311 508
Fragments arranged by size:
1691 508 311 280
BtsI GCAGTG_nn'
Cuts at: 0 1443 2790
Size: 1443 1347
DrdI GACnn_nn'nnGTC
Cuts at: 0 1077 2790
Size: 1077 1713
EaeI y'GGCC_r
Cuts at: 0 533 930 1820 1829 2565 2775 2790
Size: 533 397 890 9 736 210 15
Fragments arranged by size:
890 736 533 397 210 15 9
EagI C'GGCC_G
Cuts at: 0 2775 2790
Size: 2775 15
EarI CTCTTCn'nnn_
Cuts at: 0 1155 1400 2790
Size: 1155 245 1390
Fragments arranged by size:
1390 1155 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 627 2502 2555 2790
Size: 627 1875 53 235
Fragments arranged by size:
1875 627 235 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 862 1372 1501 1608 2320 2601 2746
Size: 169 693 510 129 107 712 281 145
Cuts at: 2746 2790
Size: 44
Fragments arranged by size:
712 693 510 281 169 145 129 107
44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1101 1575 2790
Size: 1101 474 1215
Fragments arranged by size:
1215 1101 474
EcoO109I rG'GnC_Cy
Cuts at: 0 84 144 617 1064 1259 1260 1384 1568
Size: 84 60 473 447 195 1 124 184
Cuts at: 1568 1599 1770 1980 2148 2429 2574 2611 2632
Size: 31 171 210 168 281 145 37 21
Cuts at: 2632 2744 2790
Size: 112 46
Fragments arranged by size:
473 447 281 210 195 184 171 168
145 124 112 84 60 46 37 31
21 1
EcoRV GAT'ATC
Cuts at: 0 1641 2790
Size: 1641 1149
FseI GG_CCGG'CC
Cuts at: 0 2664 2790
Size: 2664 126
HaeII r_GCGC'y
Cuts at: 0 1853 2383 2790
Size: 1853 530 407
Fragments arranged by size:
1853 530 407
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 689 721 2790
Size: 689 32 2069
Fragments arranged by size:
2069 689 32
HindIII A'AGCT_T
Cuts at: 0 844 2790
Size: 844 1946
KasI G'GCGC_C
Cuts at: 0 1849 2790
Size: 1849 941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 2790
Size: 551 2239
MscI TGG'CCA
Cuts at: 0 535 1822 2790
Size: 535 1287 968
Fragments arranged by size:
1287 968 535
MslI CAynn'nnrTG
Cuts at: 0 1318 2790
Size: 1318 1472
MspA1I CmG'CkG
Cuts at: 0 571 1200 1213 2451 2790
Size: 571 629 13 1238 339
Fragments arranged by size:
1238 629 571 339 13
NaeI GCC'GGC
Cuts at: 0 2662 2790
Size: 2662 128
NarI GG'CG_CC
Cuts at: 0 1850 2790
Size: 1850 940
NcoI C'CATG_G
Cuts at: 0 1691 2790
Size: 1691 1099
NgoMIV G'CCGG_C
Cuts at: 0 2660 2790
Size: 2660 130
PasI CC'CwG_GG
Cuts at: 0 389 868 1741 1845 2153 2790
Size: 389 479 873 104 308 637
Fragments arranged by size:
873 637 479 389 308 104
PflMI CCAn_nnn'nTGG
Cuts at: 0 321 687 1609 2790
Size: 321 366 922 1181
Fragments arranged by size:
1181 922 366 321
PfoI T'CCnGG_A
Cuts at: 0 1400 2790
Size: 1400 1390
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 2790
Size: 1021 32 1737
Fragments arranged by size:
1737 1021 32
PpuMI rG'GwC_Cy
Cuts at: 0 84 1384 1599 1770 2148 2611 2632 2790
Size: 84 1300 215 171 378 463 21 158
Fragments arranged by size:
1300 463 378 215 171 158 84 21
PspOMI G'GGCC_C
Cuts at: 0 616 1259 1980 2227 2573 2743 2790
Size: 616 643 721 247 346 170 47
Fragments arranged by size:
721 643 616 346 247 170 47
PspXI vC'TCGA_Gb
Cuts at: 0 2594 2790
Size: 2594 196
PstI C_TGCA'G
Cuts at: 0 171 470 2790
Size: 171 299 2320
Fragments arranged by size:
2320 299 171
PvuII CAG'CTG
Cuts at: 0 571 2790
Size: 571 2219
SanDI GG'GwC_CC
Cuts at: 0 2148 2632 2790
Size: 2148 484 158
Fragments arranged by size:
2148 484 158
SfcI C'TryA_G
Cuts at: 0 167 466 596 1419 2790
Size: 167 299 130 823 1371
Fragments arranged by size:
1371 823 299 167 130
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1828 2790
Size: 1828 962
SfoI GGC'GCC
Cuts at: 0 1851 2790
Size: 1851 939
SmaI CCC'GGG
Cuts at: 0 232 2274 2637 2790
Size: 232 2042 363 153
Fragments arranged by size:
2042 363 232 153
SmlI C'TyrA_G
Cuts at: 0 2594 2790
Size: 2594 196
StuI AGG'CCT
Cuts at: 0 685 904 2790
Size: 685 219 1886
Fragments arranged by size:
1886 685 219
StyI C'CwwG_G
Cuts at: 0 612 686 815 1326 1691 2003 2183 2790
Size: 612 74 129 511 365 312 180 607
Fragments arranged by size:
612 607 511 365 312 180 129 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 535 976 1116 1243 2790
Size: 535 441 140 127 1547
Fragments arranged by size:
1547 535 441 140 127
TatI w'GTAC_w
Cuts at: 0 557 2790
Size: 557 2233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 662 694 817 849 2790
Size: 229 32 401 32 123 32 1941
Fragments arranged by size:
1941 401 229 123 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 703 2790
Size: 703 2087
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1610 1632 2790
Size: 1610 22 1158
Fragments arranged by size:
1610 1158 22
XhoI C'TCGA_G
Cuts at: 0 2594 2790
Size: 2594 196
XmaI C'CCGG_G
Cuts at: 0 230 2272 2635 2790
Size: 230 2042 363 155
Fragments arranged by size:
2042 363 230 155
XmnI GAAnn'nnTTC
Cuts at: 0 427 2790
Size: 427 2363
ZraI GAC'GTC
Cuts at: 0 1903 2703 2790
Size: 1903 800 87
Fragments arranged by size:
1903 800 87
Enzymes that do cut:
AarI AatII AccI AcuI AloI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I
BspEI BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI
BtgI BtsI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRV FseI HaeII Hin4I HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
PasI PflMI PfoI PpiI PpuMI PspOMI PspXI PstI
PvuII SanDI SfcI SfiI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BciVI BclI BfrBI BglII BlpI BmtI BpuEI BsaAI
BsiHKAI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EcoICRI EcoRI FalI FspI FspAI HincII HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PshAI PsiI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XbaI