Restriction map for
pF1KSDA1874
(Linear) (Six-base) MAPSORT of: KIAA1874.seq Check: 1592 from: 1 to: 1563
KIAA1874.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 578 1563
Size: 578 985
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 638 1323 1563
Size: 353 285 685 240
Fragments arranged by size:
685 353 285 240
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 414 1563
Size: 414 1149
ApoI r'AATT_y
Cuts at: 0 540 884 953 1289 1373 1563
Size: 540 344 69 336 84 190
Fragments arranged by size:
540 344 336 190 84 69
BamHI G'GATC_C
Cuts at: 0 186 1563
Size: 186 1377
BanII G_rGCy'C
Cuts at: 0 39 1351 1563
Size: 39 1312 212
Fragments arranged by size:
1312 212 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 35 626 1563
Size: 35 591 937
Fragments arranged by size:
937 591 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 818 1067 1151 1235 1319 1563
Size: 232 586 249 84 84 84 244
Fragments arranged by size:
586 249 244 232 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1563
Size: 322 1241
BsaI GGTCTCn'nnnn_
Cuts at: 0 963 1563
Size: 963 600
BsaWI w'CCGG_w
Cuts at: 0 1465 1563
Size: 1465 98
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 1563
Size: 184 1379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1045 1533 1563
Size: 176 869 488 30
Fragments arranged by size:
869 488 176 30
BsiHKAI G_wGCw'C
Cuts at: 0 39 79 1267 1351 1563
Size: 39 40 1188 84 212
Fragments arranged by size:
1188 212 84 40 39
BsmI GAATG_Cn'
Cuts at: 0 901 910 985 1237 1563
Size: 901 9 75 252 326
Fragments arranged by size:
901 326 252 75 9
Bsp1286I G_dGCh'C
Cuts at: 0 39 79 1267 1351 1563
Size: 39 40 1188 84 212
Fragments arranged by size:
1188 212 84 40 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 823 1563
Size: 823 740
BsrDI GCAATG_nn'
Cuts at: 0 825 990 1176 1563
Size: 825 165 186 387
Fragments arranged by size:
825 387 186 165
BstAPI GCAn_nnn'nTGC
Cuts at: 0 905 1563
Size: 905 658
BstXI CCAn_nnnn'nTGG
Cuts at: 0 173 1563
Size: 173 1390
BstYI r'GATC_y
Cuts at: 0 118 186 637 1563
Size: 118 68 451 926
Fragments arranged by size:
926 451 118 68
BstZ17I GTA'TAC
Cuts at: 0 579 1563
Size: 579 984
BtgI C'CryG_G
Cuts at: 0 152 1563
Size: 152 1411
BtsI GCAGTG_nn'
Cuts at: 0 906 1242 1563
Size: 906 336 321
Fragments arranged by size:
906 336 321
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 807 842 972 1007 1563
Size: 807 35 130 35 556
Fragments arranged by size:
807 556 130 35 35
EaeI y'GGCC_r
Cuts at: 0 113 149 1563
Size: 113 36 1414
Fragments arranged by size:
1414 113 36
EarI CTCTTCn'nnn_
Cuts at: 0 67 77 757 1547 1563
Size: 67 10 680 790 16
Fragments arranged by size:
790 680 67 16 10
EcoICRI GAG'CTC
Cuts at: 0 37 1349 1563
Size: 37 1312 214
Fragments arranged by size:
1312 214 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 353 638 818 1067 1151 1235 1319
Size: 232 121 285 180 249 84 84 84
Cuts at: 1319 1323 1563
Size: 4 240
Fragments arranged by size:
285 249 240 232 180 121 84 84
84 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 29 1563
Size: 29 1534
EcoRI G'AATT_C
Cuts at: 0 540 884 953 1289 1373 1563
Size: 540 344 69 336 84 190
Fragments arranged by size:
540 344 336 190 84 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 481 933 1563
Size: 481 452 630
Fragments arranged by size:
630 481 452
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1254 1286 1563
Size: 1254 32 277
Fragments arranged by size:
1254 277 32
HindIII A'AGCT_T
Cuts at: 0 298 588 666 1082 1503 1563
Size: 298 290 78 416 421 60
Fragments arranged by size:
421 416 298 290 78 60
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 942 1563
Size: 942 621
MscI TGG'CCA
Cuts at: 0 151 1563
Size: 151 1412
MslI CAynn'nnrTG
Cuts at: 0 535 569 1230 1563
Size: 535 34 661 333
Fragments arranged by size:
661 535 333 34
NcoI C'CATG_G
Cuts at: 0 152 1563
Size: 152 1411
NdeI CA'TA_TG
Cuts at: 0 1227 1563
Size: 1227 336
PflMI CCAn_nnn'nTGG
Cuts at: 0 273 1563
Size: 273 1290
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 1563
Size: 217 32 1314
Fragments arranged by size:
1314 217 32
PsiI TTA'TAA
Cuts at: 0 710 1145 1563
Size: 710 435 418
Fragments arranged by size:
710 435 418
SacI G_AGCT'C
Cuts at: 0 39 1351 1563
Size: 39 1312 212
Fragments arranged by size:
1312 212 39
SapI GCTCTTCn'nnn_
Cuts at: 0 67 1547 1563
Size: 67 1480 16
Fragments arranged by size:
1480 67 16
SfcI C'TryA_G
Cuts at: 0 219 1563
Size: 219 1344
SmlI C'TyrA_G
Cuts at: 0 301 1563
Size: 301 1262
StyI C'CwwG_G
Cuts at: 0 152 1563
Size: 152 1411
TatI w'GTAC_w
Cuts at: 0 1012 1563
Size: 1012 551
XmnI GAAnn'nnTTC
Cuts at: 0 589 916 1336 1563
Size: 589 327 420 227
Fragments arranged by size:
589 420 327 227
Enzymes that do cut:
AccI AcuI AlfI ApoI BamHI BanII BbsI BpmI
BpuEI BsaI BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BstAPI BstXI BstYI BstZ17I BtgI BtsI
CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoRI FalI
Hin4I HindIII MmeI MscI MslI NcoI NdeI PflMI
PpiI PsiI SacI SapI SfcI SmlI StyI TatI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaXI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII Bsu36I BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoO109I EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI ZraI