Restriction map for
pF1KA1877
(Linear) (Six-base) MAPSORT of: KIAA1877.seq Check: 2928 from: 1 to: 1587
KIAA1877.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AatII G_ACGT'C
Cuts at: 0 1094 1587
Size: 1094 493
AflIII A'CryG_T
Cuts at: 0 551 982 1355 1587
Size: 551 431 373 232
Fragments arranged by size:
551 431 373 232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1366 1574 1587
Size: 1366 208 13
Fragments arranged by size:
1366 208 13
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 622 654 1587
Size: 622 32 933
Fragments arranged by size:
933 622 32
AlwNI CAG_nnn'CTG
Cuts at: 0 237 753 1044 1482 1587
Size: 237 516 291 438 105
Fragments arranged by size:
516 438 291 237 105
ApaI G_GGCC'C
Cuts at: 0 300 1587
Size: 300 1287
ApaLI G'TGCA_C
Cuts at: 0 1351 1549 1587
Size: 1351 198 38
Fragments arranged by size:
1351 198 38
ApoI r'AATT_y
Cuts at: 0 1234 1587
Size: 1234 353
AseI AT'TA_AT
Cuts at: 0 1031 1587
Size: 1031 556
AvaI C'yCGr_G
Cuts at: 0 457 1435 1587
Size: 457 978 152
Fragments arranged by size:
978 457 152
BanI G'GyrC_C
Cuts at: 0 187 527 796 804 830 837 1587
Size: 187 340 269 8 26 7 750
Fragments arranged by size:
750 340 269 187 26 8 7
BanII G_rGCy'C
Cuts at: 0 62 203 300 1587
Size: 62 141 97 1287
Fragments arranged by size:
1287 141 97 62
BbeI G_GCGC'C
Cuts at: 0 191 808 834 1587
Size: 191 617 26 753
Fragments arranged by size:
753 617 191 26
BglI GCCn_nnn'nGGC
Cuts at: 0 417 1587
Size: 417 1170
BlpI GC'TnA_GC
Cuts at: 0 107 736 1587
Size: 107 629 851
Fragments arranged by size:
851 629 107
Bme1580I G_kGCm'C
Cuts at: 0 300 747 842 851 1355 1495 1553 1587
Size: 300 447 95 9 504 140 58 34
Fragments arranged by size:
504 447 300 140 95 58 34 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 294 1587
Size: 294 1293
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 374 1587
Size: 374 1213
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 299 1587
Size: 157 142 1288
Fragments arranged by size:
1288 157 142
Bpu10I CC'TnA_GC
Cuts at: 0 853 1587
Size: 853 734
BsaI GGTCTCn'nnnn_
Cuts at: 0 133 275 1587
Size: 133 142 1312
Fragments arranged by size:
1312 142 133
BsaAI yAC'GTr
Cuts at: 0 552 985 1356 1587
Size: 552 433 371 231
Fragments arranged by size:
552 433 371 231
BsaBI GATnn'nnATC
Cuts at: 0 79 1508 1587
Size: 79 1429 79
Fragments arranged by size:
1429 79 79
BsaHI Gr'CG_yC
Cuts at: 0 188 805 831 1091 1587
Size: 188 617 26 260 496
Fragments arranged by size:
617 496 260 188 26
BsaWI w'CCGG_w
Cuts at: 0 530 1155 1587
Size: 530 625 432
Fragments arranged by size:
625 530 432
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 522 574 579 1587
Size: 52 470 52 5 1008
Fragments arranged by size:
1008 470 52 52 5
BseYI C'CCAG_C
Cuts at: 0 118 252 819 1054 1587
Size: 118 134 567 235 533
Fragments arranged by size:
567 533 235 134 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 479 847 1362 1413 1495 1544 1587
Size: 181 298 368 515 51 82 49 43
Fragments arranged by size:
515 368 298 181 82 51 49 43
BsiHKAI G_wGCw'C
Cuts at: 0 1355 1553 1587
Size: 1355 198 34
Fragments arranged by size:
1355 198 34
BsmI GAATG_Cn'
Cuts at: 0 38 1587
Size: 38 1549
BsmBI CGTCTCn'nnnn_
Cuts at: 0 639 1587
Size: 639 948
Bsp1286I G_dGCh'C
Cuts at: 0 62 203 300 747 842 851 1355 1495
Size: 62 141 97 447 95 9 504 140
Cuts at: 1495 1553 1587
Size: 58 34
Fragments arranged by size:
504 447 141 140 97 95 62 58
34 9
BspHI T'CATG_A
Cuts at: 0 948 1328 1587
Size: 948 380 259
Fragments arranged by size:
948 380 259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 1587
Size: 293 1294
BsrDI GCAATG_nn'
Cuts at: 0 25 1587
Size: 25 1562
BsrFI r'CCGG_y
Cuts at: 0 156 256 614 1587
Size: 156 100 358 973
Fragments arranged by size:
973 358 156 100
BssHII G'CGCG_C
Cuts at: 0 773 775 1587
Size: 773 2 812
Fragments arranged by size:
812 773 2
BssSI C'ACGA_G
Cuts at: 0 1405 1587
Size: 1405 182
BstAPI GCAn_nnn'nTGC
Cuts at: 0 705 1587
Size: 705 882
BtgI C'CryG_G
Cuts at: 0 472 714 870 1587
Size: 472 242 156 717
Fragments arranged by size:
717 472 242 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 685 1495 1587
Size: 685 810 92
Fragments arranged by size:
810 685 92
BtsI GCAGTG_nn'
Cuts at: 0 1551 1587
Size: 1551 36
DraIII CAC_nnn'GTG
Cuts at: 0 705 1587
Size: 705 882
EarI CTCTTCn'nnn_
Cuts at: 0 517 642 927 1587
Size: 517 125 285 660
Fragments arranged by size:
660 517 285 125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 299 1587
Size: 157 142 1288
Fragments arranged by size:
1288 157 142
EcoNI CCTnn'n_nnAGG
Cuts at: 0 664 1587
Size: 664 923
EcoO109I rG'GnC_Cy
Cuts at: 0 216 274 602 1110 1587
Size: 216 58 328 508 477
Fragments arranged by size:
508 477 328 216 58
FspI TGC'GCA
Cuts at: 0 880 1587
Size: 880 707
HaeII r_GCGC'y
Cuts at: 0 191 238 808 834 1482 1587
Size: 191 47 570 26 648 105
Fragments arranged by size:
648 570 191 105 47 26
KasI G'GCGC_C
Cuts at: 0 187 804 830 1587
Size: 187 617 26 757
Fragments arranged by size:
757 617 187 26
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 1587
Size: 536 1051
MslI CAynn'nnrTG
Cuts at: 0 1099 1330 1358 1587
Size: 1099 231 28 229
Fragments arranged by size:
1099 231 229 28
MspA1I CmG'CkG
Cuts at: 0 106 174 716 750 762 861 872 884
Size: 106 68 542 34 12 99 11 12
Cuts at: 884 1587
Size: 703
Fragments arranged by size:
703 542 106 99 68 34 12 12
11
NarI GG'CG_CC
Cuts at: 0 188 805 831 1587
Size: 188 617 26 756
Fragments arranged by size:
756 617 188 26
PflMI CCAn_nnn'nTGG
Cuts at: 0 555 1306 1587
Size: 555 751 281
Fragments arranged by size:
751 555 281
PfoI T'CCnGG_A
Cuts at: 0 134 1262 1587
Size: 134 1128 325
Fragments arranged by size:
1128 325 134
PmlI CAC'GTG
Cuts at: 0 552 985 1356 1587
Size: 552 433 371 231
Fragments arranged by size:
552 433 371 231
PpuMI rG'GwC_Cy
Cuts at: 0 1110 1587
Size: 1110 477
PspOMI G'GGCC_C
Cuts at: 0 296 1587
Size: 296 1291
PstI C_TGCA'G
Cuts at: 0 290 665 906 1587
Size: 290 375 241 681
Fragments arranged by size:
681 375 290 241
PvuII CAG'CTG
Cuts at: 0 750 762 861 884 1587
Size: 750 12 99 23 703
Fragments arranged by size:
750 703 99 23 12
SacII CC_GC'GG
Cuts at: 0 717 873 1587
Size: 717 156 714
Fragments arranged by size:
717 714 156
SanDI GG'GwC_CC
Cuts at: 0 1110 1587
Size: 1110 477
SfcI C'TryA_G
Cuts at: 0 286 661 902 1587
Size: 286 375 241 685
Fragments arranged by size:
685 375 286 241
SfoI GGC'GCC
Cuts at: 0 189 806 832 1587
Size: 189 617 26 755
Fragments arranged by size:
755 617 189 26
SmaI CCC'GGG
Cuts at: 0 459 1587
Size: 459 1128
SspI AAT'ATT
Cuts at: 0 47 1587
Size: 47 1540
StyI C'CwwG_G
Cuts at: 0 926 1587
Size: 926 661
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 266 1463 1587
Size: 266 1197 124
Fragments arranged by size:
1197 266 124
TatI w'GTAC_w
Cuts at: 0 584 1412 1587
Size: 584 828 175
Fragments arranged by size:
828 584 175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 307 1587
Size: 307 1280
XmaI C'CCGG_G
Cuts at: 0 457 1587
Size: 457 1130
ZraI GAC'GTC
Cuts at: 0 1092 1587
Size: 1092 495
Enzymes that do cut:
AatII AflIII AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AvaI BanI BanII BbeI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BssHII BssSI BstAPI BtgI
BtgZI BtsI DraIII EarI Eco57MI EcoNI EcoO109I FspI
HaeII KasI MmeI MslI MspA1I NarI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuII SacII SanDI SfcI
SfoI SmaI SspI StyI TaqII TatI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AscI AsiSI AvrII BaeI BamHI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI
BpuEI BsaXI BsiEI BsiWI BspEI BsrBI BsrGI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI
DrdI EaeI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I
XbaI XhoI XmnI