Restriction map for
pF1KA1878
(Linear) (Six-base) MAPSORT of: KIAA1878.seq Check: 6019 from: 1 to: 1317
KIAA1878.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AfeI AGC'GCT
Cuts at: 0 607 1317
Size: 607 710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 557 1317
Size: 557 760
AlwNI CAG_nnn'CTG
Cuts at: 0 259 1317
Size: 259 1058
ApaI G_GGCC'C
Cuts at: 0 295 316 1246 1317
Size: 295 21 930 71
Fragments arranged by size:
930 295 71 21
ApoI r'AATT_y
Cuts at: 0 910 1317
Size: 910 407
AscI GG'CGCG_CC
Cuts at: 0 834 1317
Size: 834 483
AvaI C'yCGr_G
Cuts at: 0 117 239 315 397 571 635 817 1245
Size: 117 122 76 82 174 64 182 428
Cuts at: 1245 1317
Size: 72
Fragments arranged by size:
428 182 174 122 117 82 76 72
64
BamHI G'GATC_C
Cuts at: 0 518 1317
Size: 518 799
BanI G'GyrC_C
Cuts at: 0 121 561 576 1317
Size: 121 440 15 741
Fragments arranged by size:
741 440 121 15
BanII G_rGCy'C
Cuts at: 0 295 316 328 404 1246 1317
Size: 295 21 12 76 842 71
Fragments arranged by size:
842 295 76 71 21 12
BbeI G_GCGC'C
Cuts at: 0 125 565 580 1317
Size: 125 440 15 737
Fragments arranged by size:
737 440 125 15
BbvCI CC'TCA_GC
Cuts at: 0 629 1161 1304 1317
Size: 629 532 143 13
Fragments arranged by size:
629 532 143 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 211 245 1317
Size: 211 34 1072
Fragments arranged by size:
1072 211 34
BglI GCCn_nnn'nGGC
Cuts at: 0 553 631 685 1317
Size: 553 78 54 632
Fragments arranged by size:
632 553 78 54
BglII A'GATC_T
Cuts at: 0 536 1317
Size: 536 781
Bme1580I G_kGCm'C
Cuts at: 0 295 316 767 1246 1317
Size: 295 21 451 479 71
Fragments arranged by size:
479 451 295 71 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 456 957 1317
Size: 456 501 360
Fragments arranged by size:
501 456 360
Bpu10I CC'TnA_GC
Cuts at: 0 629 1161 1304 1317
Size: 629 532 143 13
Fragments arranged by size:
629 532 143 13
BsaI GGTCTCn'nnnn_
Cuts at: 0 451 991 1317
Size: 451 540 326
Fragments arranged by size:
540 451 326
BsaHI Gr'CG_yC
Cuts at: 0 122 556 562 577 688 775 848 1317
Size: 122 434 6 15 111 87 73 469
Fragments arranged by size:
469 434 122 111 87 73 15 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 418 448 1317
Size: 418 30 869
Fragments arranged by size:
869 418 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 886 1317
Size: 886 431
BseYI C'CCAG_C
Cuts at: 0 403 1317
Size: 403 914
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 553 637 905 1317
Size: 409 144 84 268 412
Fragments arranged by size:
412 409 268 144 84
BsiEI CG_ry'CG
Cuts at: 0 300 415 592 680 1317
Size: 300 115 177 88 637
Fragments arranged by size:
637 300 177 115 88
BsiHKAI G_wGCw'C
Cuts at: 0 116 1014 1317
Size: 116 898 303
Fragments arranged by size:
898 303 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 196 1317
Size: 196 1121
Bsp1286I G_dGCh'C
Cuts at: 0 116 295 316 328 404 767 1014 1246
Size: 116 179 21 12 76 363 247 232
Cuts at: 1246 1317
Size: 71
Fragments arranged by size:
363 247 232 179 116 76 71 21
12
BspHI T'CATG_A
Cuts at: 0 993 1317
Size: 993 324
BsrBI CCG'CTC
Cuts at: 0 305 664 1317
Size: 305 359 653
Fragments arranged by size:
653 359 305
BsrFI r'CCGG_y
Cuts at: 0 162 268 282 410 587 811 857 1317
Size: 162 106 14 128 177 224 46 460
Fragments arranged by size:
460 224 177 162 128 106 46 14
BssHII G'CGCG_C
Cuts at: 0 184 186 834 1317
Size: 184 2 648 483
Fragments arranged by size:
648 483 184 2
BssSI C'ACGA_G
Cuts at: 0 18 766 1179 1317
Size: 18 748 413 138
Fragments arranged by size:
748 413 138 18
BstYI r'GATC_y
Cuts at: 0 518 536 1317
Size: 518 18 781
Fragments arranged by size:
781 518 18
BtgI C'CryG_G
Cuts at: 0 347 501 514 785 839 1317
Size: 347 154 13 271 54 478
Fragments arranged by size:
478 347 271 154 54 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 18 54 1317
Size: 18 36 1263
Fragments arranged by size:
1263 36 18
BtsI GCAGTG_nn'
Cuts at: 0 893 1317
Size: 893 424
DrdI GACnn_nn'nnGTC
Cuts at: 0 1057 1317
Size: 1057 260
EaeI y'GGCC_r
Cuts at: 0 7 297 412 487 589 610 677 1317
Size: 7 290 115 75 102 21 67 640
Fragments arranged by size:
640 290 115 102 75 67 21 7
EagI C'GGCC_G
Cuts at: 0 297 412 589 677 1317
Size: 297 115 177 88 640
Fragments arranged by size:
640 297 177 115 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 674 712 859 1317
Size: 674 38 147 458
Fragments arranged by size:
674 458 147 38
EcoO109I rG'GnC_Cy
Cuts at: 0 23 292 756 1242 1317
Size: 23 269 464 486 75
Fragments arranged by size:
486 464 269 75 23
EcoRI G'AATT_C
Cuts at: 0 910 1317
Size: 910 407
HaeII r_GCGC'y
Cuts at: 0 125 169 259 565 580 588 609 1317
Size: 125 44 90 306 15 8 21 708
Fragments arranged by size:
708 306 125 90 44 21 15 8
KasI G'GCGC_C
Cuts at: 0 121 561 576 1317
Size: 121 440 15 741
Fragments arranged by size:
741 440 121 15
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 206 461 1317
Size: 35 171 255 856
Fragments arranged by size:
856 255 171 35
MscI TGG'CCA
Cuts at: 0 612 1317
Size: 612 705
MslI CAynn'nnrTG
Cuts at: 0 73 1317
Size: 73 1244
MspA1I CmG'CkG
Cuts at: 0 81 349 393 407 492 503 628 780
Size: 81 268 44 14 85 11 125 152
Cuts at: 780 787 841 1048 1317
Size: 7 54 207 269
Fragments arranged by size:
269 268 207 152 125 85 81 54
44 14 11 7
NaeI GCC'GGC
Cuts at: 0 164 284 412 589 1317
Size: 164 120 128 177 728
Fragments arranged by size:
728 177 164 128 120
NarI GG'CG_CC
Cuts at: 0 122 562 577 1317
Size: 122 440 15 740
Fragments arranged by size:
740 440 122 15
NgoMIV G'CCGG_C
Cuts at: 0 162 282 410 587 1317
Size: 162 120 128 177 730
Fragments arranged by size:
730 177 162 128 120
PasI CC'CwG_GG
Cuts at: 0 216 1317
Size: 216 1101
PflMI CCAn_nnn'nTGG
Cuts at: 0 259 1317
Size: 259 1058
PspOMI G'GGCC_C
Cuts at: 0 291 312 1242 1317
Size: 291 21 930 75
Fragments arranged by size:
930 291 75 21
PvuII CAG'CTG
Cuts at: 0 407 1048 1317
Size: 407 641 269
Fragments arranged by size:
641 407 269
SacII CC_GC'GG
Cuts at: 0 350 504 788 842 1317
Size: 350 154 284 54 475
Fragments arranged by size:
475 350 284 154 54
ScaI AGT'ACT
Cuts at: 0 97 1317
Size: 97 1220
SfcI C'TryA_G
Cuts at: 0 900 1005 1089 1317
Size: 900 105 84 228
Fragments arranged by size:
900 228 105 84
SfoI GGC'GCC
Cuts at: 0 123 563 578 1317
Size: 123 440 15 739
Fragments arranged by size:
739 440 123 15
SmaI CCC'GGG
Cuts at: 0 119 241 317 637 1317
Size: 119 122 76 320 680
Fragments arranged by size:
680 320 122 119 76
SrfI GCCC'GGGC
Cuts at: 0 241 317 1317
Size: 241 76 1000
Fragments arranged by size:
1000 241 76
TatI w'GTAC_w
Cuts at: 0 95 965 1317
Size: 95 870 352
Fragments arranged by size:
870 352 95
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 499 531 1317
Size: 499 32 786
Fragments arranged by size:
786 499 32
XmaI C'CCGG_G
Cuts at: 0 117 239 315 635 1317
Size: 117 122 76 320 682
Fragments arranged by size:
682 320 122 117 76
XmnI GAAnn'nnTTC
Cuts at: 0 475 910 1317
Size: 475 435 407
Fragments arranged by size:
475 435 407
Enzymes that do cut:
AfeI AlfI AlwNI ApaI ApoI AscI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BglI BglII Bme1580I
BmrI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrFI BssHII
BssSI BstYI BtgI BtgZI BtsI DrdI EaeI EagI
EciI EcoO109I EcoRI HaeII KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV PasI PflMI PspOMI PvuII
SacII ScaI SfcI SfoI SmaI SrfI TatI TstI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI ApaLI AseI AsiSI AvrII
BaeI BbsI BciVI BclI BfrBI BlpI BmgBI BmtI
BplI BpmI BpuEI BsaAI BsaBI BsaWI BsiWI BsmI
BspEI BspMI BsrDI BsrGI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraI DraIII EarI EcoICRI
Eco57MI EcoNI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII Tth111I XbaI XcmI
XhoI ZraI