Restriction map for
pF1KA1880
(Linear) (Six-base) MAPSORT of: KIAA1880.seq Check: 7168 from: 1 to: 1836
KIAA1880.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AatII G_ACGT'C
Cuts at: 0 113 1836
Size: 113 1723
AclI AA'CG_TT
Cuts at: 0 135 1836
Size: 135 1701
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 707 1836
Size: 242 465 1129
Fragments arranged by size:
1129 465 242
AflIII A'CryG_T
Cuts at: 0 1384 1836
Size: 1384 452
AleI CACnn'nnGTG
Cuts at: 0 118 1836
Size: 118 1718
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 186 1836
Size: 186 1650
ApaLI G'TGCA_C
Cuts at: 0 55 1836
Size: 55 1781
ApoI r'AATT_y
Cuts at: 0 230 1836
Size: 230 1606
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1000 1033 1836
Size: 1000 33 803
Fragments arranged by size:
1000 803 33
BanI G'GyrC_C
Cuts at: 0 79 170 932 1767 1836
Size: 79 91 762 835 69
Fragments arranged by size:
835 762 91 79 69
BanII G_rGCy'C
Cuts at: 0 852 1836
Size: 852 984
BbeI G_GCGC'C
Cuts at: 0 1771 1836
Size: 1771 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1738 1772 1836
Size: 1738 34 64
Fragments arranged by size:
1738 64 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1001 1836
Size: 1001 835
BclI T'GATC_A
Cuts at: 0 48 1836
Size: 48 1788
BglI GCCn_nnn'nGGC
Cuts at: 0 959 1836
Size: 959 877
Bme1580I G_kGCm'C
Cuts at: 0 59 1836
Size: 59 1777
BmrI ACTGGGnnnn_n'
Cuts at: 0 299 1377 1836
Size: 299 1078 459
Fragments arranged by size:
1078 459 299
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 365 826 1836
Size: 365 461 1010
Fragments arranged by size:
1010 461 365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1776 1836
Size: 1776 60
Bpu10I CC'TnA_GC
Cuts at: 0 506 1836
Size: 506 1330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 1267 1836
Size: 504 763 569
Fragments arranged by size:
763 569 504
BsaBI GATnn'nnATC
Cuts at: 0 914 1836
Size: 914 922
BsaHI Gr'CG_yC
Cuts at: 0 110 1768 1836
Size: 110 1658 68
Fragments arranged by size:
1658 110 68
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 839 869 1798 1828 1836
Size: 839 30 929 30 8
Fragments arranged by size:
929 839 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 334 1836
Size: 334 1502
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 1836
Size: 384 1452
BsiHKAI G_wGCw'C
Cuts at: 0 59 1836
Size: 59 1777
BsmI GAATG_Cn'
Cuts at: 0 209 259 507 1836
Size: 209 50 248 1329
Fragments arranged by size:
1329 248 209 50
Bsp1286I G_dGCh'C
Cuts at: 0 59 852 1836
Size: 59 793 984
Fragments arranged by size:
984 793 59
BspHI T'CATG_A
Cuts at: 0 686 1836
Size: 686 1150
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1142 1836
Size: 1142 694
BsrDI GCAATG_nn'
Cuts at: 0 827 1836
Size: 827 1009
BsrFI r'CCGG_y
Cuts at: 0 1031 1735 1823 1836
Size: 1031 704 88 13
Fragments arranged by size:
1031 704 88 13
BstAPI GCAn_nnn'nTGC
Cuts at: 0 672 1288 1836
Size: 672 616 548
Fragments arranged by size:
672 616 548
BstBI TT'CG_AA
Cuts at: 0 1161 1836
Size: 1161 675
BtgI C'CryG_G
Cuts at: 0 1268 1529 1836
Size: 1268 261 307
Fragments arranged by size:
1268 307 261
BtsI GCAGTG_nn'
Cuts at: 0 41 248 1836
Size: 41 207 1588
Fragments arranged by size:
1588 207 41
DraI TTT'AAA
Cuts at: 0 560 1836
Size: 560 1276
DraIII CAC_nnn'GTG
Cuts at: 0 1206 1836
Size: 1206 630
EaeI y'GGCC_r
Cuts at: 0 1733 1836
Size: 1733 103
EarI CTCTTCn'nnn_
Cuts at: 0 23 1836
Size: 23 1813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 707 1776 1836
Size: 242 465 1069 60
Fragments arranged by size:
1069 465 242 60
HaeII r_GCGC'y
Cuts at: 0 1771 1836
Size: 1771 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 859 891 1018 1050 1836
Size: 370 32 457 32 127 32 786
Fragments arranged by size:
786 457 370 127 32 32 32
HincII GTy'rAC
Cuts at: 0 470 1836
Size: 470 1366
HpaI GTT'AAC
Cuts at: 0 470 1836
Size: 470 1366
KasI G'GCGC_C
Cuts at: 0 1767 1836
Size: 1767 69
MfeI C'AATT_G
Cuts at: 0 938 1286 1836
Size: 938 348 550
Fragments arranged by size:
938 550 348
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1303 1829 1836
Size: 1303 526 7
Fragments arranged by size:
1303 526 7
MslI CAynn'nnrTG
Cuts at: 0 77 118 356 490 763 1054 1836
Size: 77 41 238 134 273 291 782
Fragments arranged by size:
782 291 273 238 134 77 41
NarI GG'CG_CC
Cuts at: 0 1768 1836
Size: 1768 68
NcoI C'CATG_G
Cuts at: 0 1268 1529 1836
Size: 1268 261 307
Fragments arranged by size:
1268 307 261
NspI r_CATG'y
Cuts at: 0 489 810 1388 1836
Size: 489 321 578 448
Fragments arranged by size:
578 489 448 321
PciI A'CATG_T
Cuts at: 0 1384 1836
Size: 1384 452
PfoI T'CCnGG_A
Cuts at: 0 749 1836
Size: 749 1087
PshAI GACnn'nnGTC
Cuts at: 0 732 1836
Size: 732 1104
PstI C_TGCA'G
Cuts at: 0 36 255 1153 1836
Size: 36 219 898 683
Fragments arranged by size:
898 683 219 36
SfcI C'TryA_G
Cuts at: 0 32 251 1149 1485 1836
Size: 32 219 898 336 351
Fragments arranged by size:
898 351 336 219 32
SfoI GGC'GCC
Cuts at: 0 1769 1836
Size: 1769 67
SmlI C'TyrA_G
Cuts at: 0 519 1246 1836
Size: 519 727 590
Fragments arranged by size:
727 590 519
SphI G_CATG'C
Cuts at: 0 810 1836
Size: 810 1026
SspI AAT'ATT
Cuts at: 0 178 1836
Size: 178 1658
StuI AGG'CCT
Cuts at: 0 720 1836
Size: 720 1116
StyI C'CwwG_G
Cuts at: 0 1268 1529 1836
Size: 1268 261 307
Fragments arranged by size:
1268 307 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 950 1037 1836
Size: 950 87 799
Fragments arranged by size:
950 799 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1399 1836
Size: 1399 437
ZraI GAC'GTC
Cuts at: 0 111 1836
Size: 111 1725
Enzymes that do cut:
AatII AclI AcuI AflIII AleI AlfI ApaLI ApoI
BaeI BanI BanII BbeI BcgI BciVI BclI BglI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrFI BstAPI BstBI BtgI BtsI DraI DraIII
EaeI EarI Eco57MI HaeII Hin4I HincII HpaI KasI
MfeI MmeI MslI NarI NcoI NspI PciI PfoI
PshAI PstI SfcI SfoI SmlI SphI SspI StuI
StyI TaqII XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflII AgeI AhdI AloI
AlwNI ApaI AscI AseI AsiSI AvaI AvrII BamHI
BbsI BbvCI BfrBI BglII BlpI BmgBI BmtI BsaI
BsaAI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BsrBI
BsrGI BssHII BssSI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HindIII
KpnI MluI MscI MspA1I NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI