Restriction map for
pF1KA1883
(Linear) (Six-base) MAPSORT of: KIAA1883.seq Check: 4486 from: 1 to: 4380
KIAA1883.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 776 1191 1491 4380
Size: 776 415 300 2889
Fragments arranged by size:
2889 776 415 300
AccI GT'mk_AC
Cuts at: 0 299 3413 4076 4380
Size: 299 3114 663 304
Fragments arranged by size:
3114 663 304 299
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 673 1147 1181 4380
Size: 245 428 474 34 3199
Fragments arranged by size:
3199 474 428 245 34
AfeI AGC'GCT
Cuts at: 0 2918 4380
Size: 2918 1462
AhdI GACnn_n'nnGTC
Cuts at: 0 3006 4380
Size: 3006 1374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 832 1831 4380
Size: 832 999 2549
Fragments arranged by size:
2549 999 832
AlwNI CAG_nnn'CTG
Cuts at: 0 288 396 588 1204 2586 3061 3214 3310
Size: 288 108 192 616 1382 475 153 96
Cuts at: 3310 4380
Size: 1070
Fragments arranged by size:
1382 1070 616 475 288 192 153 108
96
ApaI G_GGCC'C
Cuts at: 0 515 1044 2286 2362 2540 2844 3086 3540
Size: 515 529 1242 76 178 304 242 454
Cuts at: 3540 3613 3742 3907 4347 4380
Size: 73 129 165 440 33
Fragments arranged by size:
1242 529 515 454 440 304 242 178
165 129 76 73 33
ApaLI G'TGCA_C
Cuts at: 0 787 4380
Size: 787 3593
ApoI r'AATT_y
Cuts at: 0 211 4380
Size: 211 4169
AvaI C'yCGr_G
Cuts at: 0 682 710 934 943 1277 1329 1429 1834
Size: 682 28 224 9 334 52 100 405
Cuts at: 1834 2110 2188 2233 2279 2473 2514 2532 2540
Size: 276 78 45 46 194 41 18 8
Cuts at: 2540 2645 2680 2700 2854 3100 3256 3277 3325
Size: 105 35 20 154 246 156 21 48
Cuts at: 3325 3495 3520 3532 3598 3613 3729 3907 4129
Size: 170 25 12 66 15 116 178 222
Cuts at: 4129 4326 4340 4380
Size: 197 14 40
Fragments arranged by size:
682 405 334 276 246 224 222 197
194 178 170 156 154 116 105 100
78 66 52 48 46 45 41 40
35 28 25 21 20 18 15 14
12 9 8
BamHI G'GATC_C
Cuts at: 0 911 4380
Size: 911 3469
BanI G'GyrC_C
Cuts at: 0 13 930 1479 1846 2220 3291 3876 3930
Size: 13 917 549 367 374 1071 585 54
Cuts at: 3930 4380
Size: 450
Fragments arranged by size:
1071 917 585 549 450 374 367 54
13
BanII G_rGCy'C
Cuts at: 0 99 497 515 672 941 953 1044 1652
Size: 99 398 18 157 269 12 91 608
Cuts at: 1652 2146 2286 2362 2469 2540 2808 2844 3078
Size: 494 140 76 107 71 268 36 234
Cuts at: 3078 3086 3165 3276 3303 3345 3533 3540 3613
Size: 8 79 111 27 42 188 7 73
Cuts at: 3613 3742 3907 4347 4380
Size: 129 165 440 33
Fragments arranged by size:
608 494 440 398 269 268 234 188
165 157 140 129 111 107 99 91
79 76 73 71 42 36 33 27
18 12 8 7
BbeI G_GCGC'C
Cuts at: 0 17 934 1850 2224 3295 3880 4380
Size: 17 917 916 374 1071 585 500
Fragments arranged by size:
1071 917 916 585 500 374 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 1961 3100 4380
Size: 1961 1139 1280
Fragments arranged by size:
1961 1280 1139
BbvCI CC'TCA_GC
Cuts at: 0 317 1797 2935 4380
Size: 317 1480 1138 1445
Fragments arranged by size:
1480 1445 1138 317
BglI GCCn_nnn'nGGC
Cuts at: 0 1251 1510 1702 1711 3224 3307 3477 3778
Size: 1251 259 192 9 1513 83 170 301
Cuts at: 3778 3799 4380
Size: 21 581
Fragments arranged by size:
1513 1251 581 301 259 192 170 83
21 9
BlpI GC'TnA_GC
Cuts at: 0 1819 4111 4380
Size: 1819 2292 269
Fragments arranged by size:
2292 1819 269
Bme1580I G_kGCm'C
Cuts at: 0 515 791 1044 1482 1694 2286 2362 2540
Size: 515 276 253 438 212 592 76 178
Cuts at: 2540 2844 3086 3355 3540 3613 3742 3907 3935
Size: 304 242 269 185 73 129 165 28
Cuts at: 3935 4347 4380
Size: 412 33
Fragments arranged by size:
592 515 438 412 304 276 269 253
242 212 185 178 165 129 76 73
33 28
BmgBI CAC'GTC
Cuts at: 0 4068 4380
Size: 4068 312
BmrI ACTGGGnnnn_n'
Cuts at: 0 652 1677 1785 2220 2758 3071 3293 3340
Size: 652 1025 108 435 538 313 222 47
Cuts at: 3340 4380
Size: 1040
Fragments arranged by size:
1040 1025 652 538 435 313 222 108
47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 255 288 291 474 1653 1959 2136 2160
Size: 255 33 3 183 1179 306 177 24
Cuts at: 2160 4380
Size: 2220
Fragments arranged by size:
2220 1179 306 255 183 177 33 24
3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 565 763 1394 2068 2492 2806 2896
Size: 538 27 198 631 674 424 314 90
Cuts at: 2896 2941 3175 3502 4051 4142 4256 4339 4380
Size: 45 234 327 549 91 114 83 41
Fragments arranged by size:
674 631 549 538 424 327 314 234
198 114 91 90 83 45 41 27
Bpu10I CC'TnA_GC
Cuts at: 0 317 382 397 698 1646 1797 2755 2935
Size: 317 65 15 301 948 151 958 180
Cuts at: 2935 4380
Size: 1445
Fragments arranged by size:
1445 958 948 317 301 180 151 65
15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 3973 4380
Size: 1111 2862 407
Fragments arranged by size:
2862 1111 407
BsaI GGTCTCn'nnnn_
Cuts at: 0 262 327 707 1738 1849 1862 2380 2386
Size: 262 65 380 1031 111 13 518 6
Cuts at: 2386 3139 3189 4056 4315 4380
Size: 753 50 867 259 65
Fragments arranged by size:
1031 867 753 518 380 262 259 111
65 65 50 13 6
BsaAI yAC'GTr
Cuts at: 0 786 4380
Size: 786 3594
BsaHI Gr'CG_yC
Cuts at: 0 14 58 931 1498 1847 2060 2221 3097
Size: 14 44 873 567 349 213 161 876
Cuts at: 3097 3292 3770 3877 4096 4352 4380
Size: 195 478 107 219 256 28
Fragments arranged by size:
876 873 567 478 349 256 219 213
195 161 107 44 28 14
BsaWI w'CCGG_w
Cuts at: 0 3459 4380
Size: 3459 921
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1219 1249 2923 2953 4380
Size: 1219 30 1674 30 1427
Fragments arranged by size:
1674 1427 1219 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 89 98 154 157 264 267 280 931
Size: 89 9 56 3 107 3 13 651
Cuts at: 931 1084 1332 1642 1939 1942 1945 1948 2112
Size: 153 248 310 297 3 3 3 164
Cuts at: 2112 2267 2455 2833 2923 3177 3282 3489 3838
Size: 155 188 378 90 254 105 207 349
Cuts at: 3838 3868 3871 3880 4252 4380
Size: 30 3 9 372 128
Fragments arranged by size:
651 378 372 349 310 297 254 248
207 188 164 155 153 128 107 105
90 89 56 30 13 9 9 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 671 923 1043 1187 1561 1730 1785 1826
Size: 671 252 120 144 374 169 55 41
Cuts at: 1826 2072 3172 3228 3310 4207 4380
Size: 246 1100 56 82 897 173
Fragments arranged by size:
1100 897 671 374 252 246 173 169
144 120 82 56 55 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1295 2034 3152 4380
Size: 1295 739 1118 1228
Fragments arranged by size:
1295 1228 1118 739
BsiEI CG_ry'CG
Cuts at: 0 35 1120 1467 2300 2324 2438 2461 3128
Size: 35 1085 347 833 24 114 23 667
Cuts at: 3128 4380
Size: 1252
Fragments arranged by size:
1252 1085 833 667 347 114 35 24
23
BsiHKAI G_wGCw'C
Cuts at: 0 497 791 941 953 1397 1634 2575 4380
Size: 497 294 150 12 444 237 941 1805
Fragments arranged by size:
1805 941 497 444 294 237 150 12
BsmI GAATG_Cn'
Cuts at: 0 771 4380
Size: 771 3609
BsmBI CGTCTCn'nnnn_
Cuts at: 0 42 598 1274 3572 4087 4311 4380
Size: 42 556 676 2298 515 224 69
Fragments arranged by size:
2298 676 556 515 224 69 42
Bsp1286I G_dGCh'C
Cuts at: 0 99 497 515 672 791 941 953 1044
Size: 99 398 18 157 119 150 12 91
Cuts at: 1044 1397 1482 1634 1652 1694 2146 2286 2362
Size: 353 85 152 18 42 452 140 76
Cuts at: 2362 2469 2540 2575 2808 2844 3078 3086 3165
Size: 107 71 35 233 36 234 8 79
Cuts at: 3165 3276 3303 3345 3355 3533 3540 3613 3742
Size: 111 27 42 10 178 7 73 129
Cuts at: 3742 3907 3935 4347 4380
Size: 165 28 412 33
Fragments arranged by size:
452 412 398 353 234 233 178 165
157 152 150 140 129 119 111 107
99 91 85 79 76 73 71 42
42 36 35 33 28 27 18 18
12 10 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 413 725 776 1191 1491 4380
Size: 413 312 51 415 300 2889
Fragments arranged by size:
2889 415 413 312 300 51
BsrBI CCG'CTC
Cuts at: 0 1242 1823 1893 2052 2139 2254 2523 3711
Size: 1242 581 70 159 87 115 269 1188
Cuts at: 3711 4380
Size: 669
Fragments arranged by size:
1242 1188 669 581 269 159 115 87
70
BsrFI r'CCGG_y
Cuts at: 0 386 1462 1494 3553 3793 3816 4380
Size: 386 1076 32 2059 240 23 564
Fragments arranged by size:
2059 1076 564 386 240 32 23
BssSI C'ACGA_G
Cuts at: 0 3420 4380
Size: 3420 960
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1318 4380
Size: 1318 3062
BstEII G'GTnAC_C
Cuts at: 0 1011 1401 4380
Size: 1011 390 2979
Fragments arranged by size:
2979 1011 390
BstXI CCAn_nnnn'nTGG
Cuts at: 0 76 375 923 3086 3180 4380
Size: 76 299 548 2163 94 1200
Fragments arranged by size:
2163 1200 548 299 94 76
BstYI r'GATC_y
Cuts at: 0 911 4380
Size: 911 3469
Bsu36I CC'TnA_GG
Cuts at: 0 1700 3650 4380
Size: 1700 1950 730
Fragments arranged by size:
1950 1700 730
BtgI C'CryG_G
Cuts at: 0 753 954 1416 1906 2162 2288 2324 2461
Size: 753 201 462 490 256 126 36 137
Cuts at: 2461 3742 3754 3898 4059 4370 4380
Size: 1281 12 144 161 311 10
Fragments arranged by size:
1281 753 490 462 311 256 201 161
144 137 126 36 12 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 209 4380
Size: 209 4171
BtsI GCAGTG_nn'
Cuts at: 0 916 3185 4380
Size: 916 2269 1195
Fragments arranged by size:
2269 1195 916
DraIII CAC_nnn'GTG
Cuts at: 0 1318 4380
Size: 1318 3062
DrdI GACnn_nn'nnGTC
Cuts at: 0 1072 2722 4072 4380
Size: 1072 1650 1350 308
Fragments arranged by size:
1650 1350 1072 308
EaeI y'GGCC_r
Cuts at: 0 32 1117 1177 1464 2150 2297 2321 2327
Size: 32 1085 60 287 686 147 24 6
Cuts at: 2327 2435 2458 2500 4380
Size: 108 23 42 1880
Fragments arranged by size:
1880 1085 686 287 147 108 60 42
32 24 23 6
EagI C'GGCC_G
Cuts at: 0 32 1117 1464 2297 2321 2435 2458 4380
Size: 32 1085 347 833 24 114 23 1922
Fragments arranged by size:
1922 1085 833 347 114 32 24 23
EarI CTCTTCn'nnn_
Cuts at: 0 1660 1768 1916 2858 4087 4380
Size: 1660 108 148 942 1229 293
Fragments arranged by size:
1660 1229 942 293 148 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 30 1081 2845 3572 3835 4261 4380
Size: 30 1051 1764 727 263 426 119
Fragments arranged by size:
1764 1051 727 426 263 119 30
EcoICRI GAG'CTC
Cuts at: 0 495 939 951 4380
Size: 495 444 12 3429
Fragments arranged by size:
3429 495 444 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 538 565 673 763 1147 1181 1394
Size: 245 293 27 108 90 384 34 213
Cuts at: 1394 2068 2492 2806 2896 2941 3175 3502 4051
Size: 674 424 314 90 45 234 327 549
Cuts at: 4051 4142 4256 4339 4380
Size: 91 114 83 41
Fragments arranged by size:
674 549 424 384 327 314 293 245
234 213 114 108 91 90 90 83
45 41 34 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2599 4380
Size: 2599 1781
EcoO109I rG'GnC_Cy
Cuts at: 0 511 512 648 687 959 1040 1074 1334
Size: 511 1 136 39 272 81 34 260
Cuts at: 1334 1671 1704 1713 1725 1830 1993 2027 2283
Size: 337 33 9 12 105 163 34 256
Cuts at: 2283 2359 2451 2536 2537 2656 2677 2715 2840
Size: 76 92 85 1 119 21 38 125
Cuts at: 2840 2841 2849 2939 2976 3021 3082 3083 3536
Size: 1 8 90 37 45 61 1 453
Cuts at: 3536 3537 3585 3609 3610 3643 3903 3904 4122
Size: 1 48 24 1 33 260 1 218
Cuts at: 4122 4276 4344 4366 4380
Size: 154 68 22 14
Fragments arranged by size:
511 453 337 272 260 260 256 218
163 154 136 125 119 105 92 90
85 81 76 68 61 48 45 39
38 37 34 34 33 33 24 22
21 14 12 9 8 1 1 1
1 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2571 4380
Size: 2571 1809
FseI GG_CCGG'CC
Cuts at: 0 1466 4380
Size: 1466 2914
FspI TGC'GCA
Cuts at: 0 808 841 4380
Size: 808 33 3539
Fragments arranged by size:
3539 808 33
FspAI rTGC'GCAy
Cuts at: 0 841 4380
Size: 841 3539
HaeII r_GCGC'y
Cuts at: 0 17 906 934 1194 1552 1586 1850 2224
Size: 17 889 28 260 358 34 264 374
Cuts at: 2224 2497 2920 3295 3493 3703 3811 3880 3953
Size: 273 423 375 198 210 108 69 73
Cuts at: 3953 4165 4380
Size: 212 215
Fragments arranged by size:
889 423 375 374 358 273 264 260
215 212 210 198 108 73 69 34
28 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 676 708 1537 1569 2107 2139 2681 2713
Size: 676 32 829 32 538 32 542 32
Cuts at: 2713 2923 2955 2992 3024 3096 3128 4380
Size: 210 32 37 32 72 32 1252
Fragments arranged by size:
1252 829 676 542 538 210 72 37
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 640 4151 4380
Size: 640 3511 229
Fragments arranged by size:
3511 640 229
KasI G'GCGC_C
Cuts at: 0 13 930 1846 2220 3291 3876 4380
Size: 13 917 916 374 1071 585 504
Fragments arranged by size:
1071 917 916 585 504 374 13
MfeI C'AATT_G
Cuts at: 0 1213 4380
Size: 1213 3167
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1556 4380
Size: 485 1071 2824
Fragments arranged by size:
2824 1071 485
MscI TGG'CCA
Cuts at: 0 2329 4380
Size: 2329 2051
MslI CAynn'nnrTG
Cuts at: 0 74 572 970 4380
Size: 74 498 398 3410
Fragments arranged by size:
3410 498 398 74
MspA1I CmG'CkG
Cuts at: 0 312 675 755 1418 1734 2290 2378 2463
Size: 312 363 80 663 316 556 88 85
Cuts at: 2463 2583 3447 3575 3744 3900 3975 4220 4284
Size: 120 864 128 169 156 75 245 64
Cuts at: 4284 4380
Size: 96
Fragments arranged by size:
864 663 556 363 316 312 245 169
156 128 120 96 88 85 80 75
64
NaeI GCC'GGC
Cuts at: 0 388 1464 1496 3555 3795 3818 4380
Size: 388 1076 32 2059 240 23 562
Fragments arranged by size:
2059 1076 562 388 240 32 23
NarI GG'CG_CC
Cuts at: 0 14 931 1847 2221 3292 3877 4380
Size: 14 917 916 374 1071 585 503
Fragments arranged by size:
1071 917 916 585 503 374 14
NcoI C'CATG_G
Cuts at: 0 2162 4380
Size: 2162 2218
NgoMIV G'CCGG_C
Cuts at: 0 386 1462 1494 3553 3793 3816 4380
Size: 386 1076 32 2059 240 23 564
Fragments arranged by size:
2059 1076 564 386 240 32 23
NspI r_CATG'y
Cuts at: 0 1105 4380
Size: 1105 3275
PasI CC'CwG_GG
Cuts at: 0 87 375 1005 1948 2844 3079 3086 3304
Size: 87 288 630 943 896 235 7 218
Cuts at: 3304 4380
Size: 1076
Fragments arranged by size:
1076 943 896 630 288 235 218 87
7
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 588 1667 3061 3310 4380
Size: 483 105 1079 1394 249 1070
Fragments arranged by size:
1394 1079 1070 483 249 105
PfoI T'CCnGG_A
Cuts at: 0 3190 4380
Size: 3190 1190
PpuMI rG'GwC_Cy
Cuts at: 0 648 959 1074 1334 1671 1725 1830 1993
Size: 648 311 115 260 337 54 105 163
Cuts at: 1993 2451 2656 2715 2849 2939 3585 3643 4380
Size: 458 205 59 134 90 646 58 737
Fragments arranged by size:
737 648 646 458 337 311 260 205
163 134 115 105 90 59 58 54
PshAI GACnn'nnGTC
Cuts at: 0 2516 4380
Size: 2516 1864
PspOMI G'GGCC_C
Cuts at: 0 511 1040 2282 2358 2536 2840 3082 3536
Size: 511 529 1242 76 178 304 242 454
Cuts at: 3536 3609 3738 3903 4343 4380
Size: 73 129 165 440 37
Fragments arranged by size:
1242 529 511 454 440 304 242 178
165 129 76 73 37
PspXI vC'TCGA_Gb
Cuts at: 0 1429 4380
Size: 1429 2951
PstI C_TGCA'G
Cuts at: 0 410 566 731 4380
Size: 410 156 165 3649
Fragments arranged by size:
3649 410 165 156
PvuI CG_AT'CG
Cuts at: 0 3128 4380
Size: 3128 1252
PvuII CAG'CTG
Cuts at: 0 1734 2583 4380
Size: 1734 849 1797
Fragments arranged by size:
1797 1734 849
SacI G_AGCT'C
Cuts at: 0 497 941 953 4380
Size: 497 444 12 3427
Fragments arranged by size:
3427 497 444 12
SacII CC_GC'GG
Cuts at: 0 756 1419 2291 2464 3745 3901 4380
Size: 756 663 872 173 1281 156 479
Fragments arranged by size:
1281 872 756 663 479 173 156
SanDI GG'GwC_CC
Cuts at: 0 1334 1671 1830 1993 2451 2715 2849 3585
Size: 1334 337 159 163 458 264 134 736
Cuts at: 3585 3643 4380
Size: 58 737
Fragments arranged by size:
1334 737 736 458 337 264 163 159
134 58
SbfI CC_TGCA'GG
Cuts at: 0 410 4380
Size: 410 3970
ScaI AGT'ACT
Cuts at: 0 1612 4380
Size: 1612 2768
SfcI C'TryA_G
Cuts at: 0 406 562 727 4380
Size: 406 156 165 3653
Fragments arranged by size:
3653 406 165 156
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1251 1510 1711 3224 4380
Size: 1251 259 201 1513 1156
Fragments arranged by size:
1513 1251 1156 259 201
SfoI GGC'GCC
Cuts at: 0 15 932 1848 2222 3293 3878 4380
Size: 15 917 916 374 1071 585 502
Fragments arranged by size:
1071 917 916 585 502 374 15
SmaI CCC'GGG
Cuts at: 0 1331 2235 2516 2534 2542 2647 2682 2702
Size: 1331 904 281 18 8 105 35 20
Cuts at: 2702 3258 3534 3731 3909 4328 4342 4380
Size: 556 276 197 178 419 14 38
Fragments arranged by size:
1331 904 556 419 281 276 197 178
105 38 35 20 18 14 8
SmlI C'TyrA_G
Cuts at: 0 710 1126 1429 3988 4380
Size: 710 416 303 2559 392
Fragments arranged by size:
2559 710 416 392 303
SrfI GCCC'GGGC
Cuts at: 0 4342 4380
Size: 4342 38
StuI AGG'CCT
Cuts at: 0 2345 4380
Size: 2345 2035
StyI C'CwwG_G
Cuts at: 0 1968 2162 2899 3067 3222 4380
Size: 1968 194 737 168 155 1158
Fragments arranged by size:
1968 1158 737 194 168 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1899 2404 3186 3277 4380
Size: 1899 505 782 91 1103
Fragments arranged by size:
1899 1103 782 505 91
TatI w'GTAC_w
Cuts at: 0 248 1610 4380
Size: 248 1362 2770
Fragments arranged by size:
2770 1362 248
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 757 789 3374 3406 4380
Size: 757 32 2585 32 974
Fragments arranged by size:
2585 974 757 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 905 2089 3510 4380
Size: 905 1184 1421 870
Fragments arranged by size:
1421 1184 905 870
XhoI C'TCGA_G
Cuts at: 0 710 1429 4380
Size: 710 719 2951
Fragments arranged by size:
2951 719 710
XmaI C'CCGG_G
Cuts at: 0 1329 2233 2514 2532 2540 2645 2680 2700
Size: 1329 904 281 18 8 105 35 20
Cuts at: 2700 3256 3532 3729 3907 4326 4340 4380
Size: 556 276 197 178 419 14 40
Fragments arranged by size:
1329 904 556 419 281 276 197 178
105 40 35 20 18 14 8
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI
FspAI HaeII Hin4I HincII KasI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII SacI SacII SanDI SbfI ScaI SfcI SfiI
SfoI SmaI SmlI SrfI StuI StyI TaqII TatI
TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AleI AloI
AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI
BfrBI BglII BmtI BsaBI BsiWI BspEI BspHI BsrDI
BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI EcoRI
EcoRV HindIII HpaI KpnI MluI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PsrI RsrII SalI SapI SexAI SgfI SgrAI SnaBI
SpeI SphI SspI SwaI Tth111I XbaI XmnI ZraI