Restriction map for
pF1KA1885
(Linear) (Six-base) MAPSORT of: KIAA1885.seq Check: 8447 from: 1 to: 3189
KIAA1885.orf
With 182 enzymes: SgfI *
November 13, 2006 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 213 2025 2721 3189
Size: 213 1812 696 468
Fragments arranged by size:
1812 696 468 213
AatII G_ACGT'C
Cuts at: 0 2459 3189
Size: 2459 730
AccI GT'mk_AC
Cuts at: 0 2459 2945 3189
Size: 2459 486 244
Fragments arranged by size:
2459 486 244
Acc65I G'GTAC_C
Cuts at: 0 338 3189
Size: 338 2851
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 1447 1908 2603 3189
Size: 794 653 461 695 586
Fragments arranged by size:
794 695 653 586 461
AflIII A'CryG_T
Cuts at: 0 1152 3189
Size: 1152 2037
AgeI A'CCGG_T
Cuts at: 0 1301 3189
Size: 1301 1888
AleI CACnn'nnGTG
Cuts at: 0 931 3189
Size: 931 2258
AlwNI CAG_nnn'CTG
Cuts at: 0 766 1669 2040 2209 2523 2601 2663 2806
Size: 766 903 371 169 314 78 62 143
Cuts at: 2806 2964 3150 3189
Size: 158 186 39
Fragments arranged by size:
903 766 371 314 186 169 158 143
78 62 39
ApaI G_GGCC'C
Cuts at: 0 296 1218 1467 1497 2513 2916 3189
Size: 296 922 249 30 1016 403 273
Fragments arranged by size:
1016 922 403 296 273 249 30
ApoI r'AATT_y
Cuts at: 0 2064 2287 3189
Size: 2064 223 902
Fragments arranged by size:
2064 902 223
AvaI C'yCGr_G
Cuts at: 0 1313 1466 2396 2711 2780 3189
Size: 1313 153 930 315 69 409
Fragments arranged by size:
1313 930 409 315 153 69
BamHI G'GATC_C
Cuts at: 0 1734 2126 2342 3189
Size: 1734 392 216 847
Fragments arranged by size:
1734 847 392 216
BanI G'GyrC_C
Cuts at: 0 338 506 709 2681 2848 3189
Size: 338 168 203 1972 167 341
Fragments arranged by size:
1972 341 338 203 168 167
BanII G_rGCy'C
Cuts at: 0 101 296 705 1218 1234 1467 1497 1505
Size: 101 195 409 513 16 233 30 8
Cuts at: 1505 2405 2513 2591 2745 2800 2843 2916 3181
Size: 900 108 78 154 55 43 73 265
Cuts at: 3181 3188 3189
Size: 7 1
Fragments arranged by size:
900 513 409 265 233 195 154 108
101 78 73 55 43 30 16 8
7 1
BbeI G_GCGC'C
Cuts at: 0 2685 3189
Size: 2685 504
BbsI GAAGACnn'nnnn_
Cuts at: 0 1288 3189
Size: 1288 1901
BbvCI CC'TCA_GC
Cuts at: 0 46 1670 2306 3189
Size: 46 1624 636 883
Fragments arranged by size:
1624 883 636 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2216 2250 3189
Size: 2216 34 939
Fragments arranged by size:
2216 939 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 436 3189
Size: 436 2753
BglI GCCn_nnn'nGGC
Cuts at: 0 2092 2610 3189
Size: 2092 518 579
Fragments arranged by size:
2092 579 518
BglII A'GATC_T
Cuts at: 0 683 2243 3189
Size: 683 1560 946
Fragments arranged by size:
1560 946 683
BlpI GC'TnA_GC
Cuts at: 0 625 3189
Size: 625 2564
Bme1580I G_kGCm'C
Cuts at: 0 296 1162 1218 1467 1497 2513 2851 2916
Size: 296 866 56 249 30 1016 338 65
Cuts at: 2916 3189
Size: 273
Fragments arranged by size:
1016 866 338 296 273 249 65 56
30
BmrI ACTGGGnnnn_n'
Cuts at: 0 732 3189
Size: 732 2457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 246 1049 1166 1348 1519 1747 2485
Size: 193 53 803 117 182 171 228 738
Cuts at: 2485 2716 3085 3127 3149 3189
Size: 231 369 42 22 40
Fragments arranged by size:
803 738 369 231 228 193 182 171
117 53 42 40 22
Bpu10I CC'TnA_GC
Cuts at: 0 46 1670 2306 2347 2737 3189
Size: 46 1624 636 41 390 452
Fragments arranged by size:
1624 636 452 390 46 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1248 2924 3189
Size: 1248 1676 265
Fragments arranged by size:
1676 1248 265
BsaI GGTCTCn'nnnn_
Cuts at: 0 403 1798 3189
Size: 403 1395 1391
Fragments arranged by size:
1395 1391 403
BsaAI yAC'GTr
Cuts at: 0 2756 3189
Size: 2756 433
BsaHI Gr'CG_yC
Cuts at: 0 2456 2682 3189
Size: 2456 226 507
Fragments arranged by size:
2456 507 226
BsaWI w'CCGG_w
Cuts at: 0 509 812 1301 3189
Size: 509 303 489 1888
Fragments arranged by size:
1888 509 489 303
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 47 77 114 144 184 214 3189
Size: 47 30 37 30 40 30 2975
Fragments arranged by size:
2975 47 40 37 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 627 747 2313 2323 3189
Size: 627 120 1566 10 866
Fragments arranged by size:
1566 866 627 120 10
BseYI C'CCAG_C
Cuts at: 0 1901 2632 2656 2909 3189
Size: 1901 731 24 253 280
Fragments arranged by size:
1901 731 280 253 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 2410 2749 3189
Size: 439 1971 339 440
Fragments arranged by size:
1971 440 439 339
BsiHKAI G_wGCw'C
Cuts at: 0 705 1505 2405 2591 2678 2800 3188 3189
Size: 705 800 900 186 87 122 388 1
Fragments arranged by size:
900 800 705 388 186 122 87 1
BsmI GAATG_Cn'
Cuts at: 0 2256 3189
Size: 2256 933
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1012 3189
Size: 1012 2177
Bsp1286I G_dGCh'C
Cuts at: 0 101 296 705 1162 1218 1234 1467 1497
Size: 101 195 409 457 56 16 233 30
Cuts at: 1497 1505 2405 2513 2591 2678 2745 2800 2843
Size: 8 900 108 78 87 67 55 43
Cuts at: 2843 2851 2916 3181 3188 3189
Size: 8 65 265 7 1
Fragments arranged by size:
900 457 409 265 233 195 108 101
87 78 67 65 56 55 43 30
16 8 8 7 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 213 1430 2025 2721 3189
Size: 213 1217 595 696 468
Fragments arranged by size:
1217 696 595 468 213
BsrBI CCG'CTC
Cuts at: 0 2382 2700 3189
Size: 2382 318 489
Fragments arranged by size:
2382 489 318
BsrDI GCAATG_nn'
Cuts at: 0 1244 3189
Size: 1244 1945
BsrFI r'CCGG_y
Cuts at: 0 881 1301 3189
Size: 881 420 1888
Fragments arranged by size:
1888 881 420
BssSI C'ACGA_G
Cuts at: 0 496 3189
Size: 496 2693
BstAPI GCAn_nnn'nTGC
Cuts at: 0 513 562 2040 2601 3189
Size: 513 49 1478 561 588
Fragments arranged by size:
1478 588 561 513 49
BstXI CCAn_nnnn'nTGG
Cuts at: 0 38 1482 3189
Size: 38 1444 1707
Fragments arranged by size:
1707 1444 38
BstYI r'GATC_y
Cuts at: 0 106 683 1734 2126 2243 2342 2978 3189
Size: 106 577 1051 392 117 99 636 211
Fragments arranged by size:
1051 636 577 392 211 117 106 99
Bsu36I CC'TnA_GG
Cuts at: 0 233 643 2516 3189
Size: 233 410 1873 673
Fragments arranged by size:
1873 673 410 233
BtgI C'CryG_G
Cuts at: 0 102 343 743 968 1376 1946 2172 3189
Size: 102 241 400 225 408 570 226 1017
Fragments arranged by size:
1017 570 408 400 241 226 225 102
BtsI GCAGTG_nn'
Cuts at: 0 664 1175 3189
Size: 664 511 2014
Fragments arranged by size:
2014 664 511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 514 549 3189
Size: 514 35 2640
Fragments arranged by size:
2640 514 35
DraIII CAC_nnn'GTG
Cuts at: 0 2929 3189
Size: 2929 260
DrdI GACnn_nn'nnGTC
Cuts at: 0 1094 1564 3189
Size: 1094 470 1625
Fragments arranged by size:
1625 1094 470
EaeI y'GGCC_r
Cuts at: 0 462 479 894 1043 2084 3189
Size: 462 17 415 149 1041 1105
Fragments arranged by size:
1105 1041 462 415 149 17
EarI CTCTTCn'nnn_
Cuts at: 0 1124 1631 2261 2579 2827 3189
Size: 1124 507 630 318 248 362
Fragments arranged by size:
1124 630 507 362 318 248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2539 3189
Size: 2539 650
EcoICRI GAG'CTC
Cuts at: 0 703 1503 2403 2589 2798 3186 3189
Size: 703 800 900 186 209 388 3
Fragments arranged by size:
900 800 703 388 209 186 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 246 794 1049 1166 1348 1447 1519
Size: 193 53 548 255 117 182 99 72
Cuts at: 1519 1747 1908 2485 2603 2716 3085 3127 3149
Size: 228 161 577 118 113 369 42 22
Cuts at: 3149 3189
Size: 40
Fragments arranged by size:
577 548 369 255 228 193 182 161
118 117 113 99 72 53 42 40
22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 642 646 2824 3189
Size: 642 4 2178 365
Fragments arranged by size:
2178 642 365 4
EcoO109I rG'GnC_Cy
Cuts at: 0 60 293 537 1214 1347 1359 1463 1493
Size: 60 233 244 677 133 12 104 30
Cuts at: 1493 1494 1898 2509 2510 2520 2817 2961 3047
Size: 1 404 611 1 10 297 144 86
Cuts at: 3047 3189
Size: 142
Fragments arranged by size:
677 611 404 297 244 233 144 142
133 104 86 60 30 12 10 1
1
FspI TGC'GCA
Cuts at: 0 1628 3189
Size: 1628 1561
HaeII r_GCGC'y
Cuts at: 0 150 2663 2685 2706 3189
Size: 150 2513 22 21 483
Fragments arranged by size:
2513 483 150 22 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 98 130 184 216 739 771 842 874
Size: 98 32 54 32 523 32 71 32
Cuts at: 874 1170 1202 2000 2032 2147 2179 2299 2331
Size: 296 32 798 32 115 32 120 32
Cuts at: 2331 3189
Size: 858
Fragments arranged by size:
858 798 523 296 120 115 98 71
54 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 831 3189
Size: 831 2358
HindIII A'AGCT_T
Cuts at: 0 776 3088 3189
Size: 776 2312 101
Fragments arranged by size:
2312 776 101
KasI G'GCGC_C
Cuts at: 0 2681 3189
Size: 2681 508
KpnI G_GTAC'C
Cuts at: 0 342 3189
Size: 342 2847
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 3189
Size: 74 3115
MscI TGG'CCA
Cuts at: 0 464 481 896 2086 3189
Size: 464 17 415 1190 1103
Fragments arranged by size:
1190 1103 464 415 17
MslI CAynn'nnrTG
Cuts at: 0 871 931 1888 3189
Size: 871 60 957 1301
Fragments arranged by size:
1301 957 871 60
MspA1I CmG'CkG
Cuts at: 0 696 1448 1578 1690 1917 3105 3150 3189
Size: 696 752 130 112 227 1188 45 39
Fragments arranged by size:
1188 752 696 227 130 112 45 39
NarI GG'CG_CC
Cuts at: 0 2682 3189
Size: 2682 507
NcoI C'CATG_G
Cuts at: 0 102 743 1376 1946 2172 3189
Size: 102 641 633 570 226 1017
Fragments arranged by size:
1017 641 633 570 226 102
NruI TCG'CGA
Cuts at: 0 1065 3189
Size: 1065 2124
NspI r_CATG'y
Cuts at: 0 1156 3189
Size: 1156 2033
PasI CC'CwG_GG
Cuts at: 0 1788 1986 2505 2844 2853 3172 3189
Size: 1788 198 519 339 9 319 17
Fragments arranged by size:
1788 519 339 319 198 17 9
PciI A'CATG_T
Cuts at: 0 1152 3189
Size: 1152 2037
PflMI CCAn_nnn'nTGG
Cuts at: 0 1377 1458 3189
Size: 1377 81 1731
Fragments arranged by size:
1731 1377 81
PfoI T'CCnGG_A
Cuts at: 0 118 3189
Size: 118 3071
PpuMI rG'GwC_Cy
Cuts at: 0 537 1898 2520 3047 3189
Size: 537 1361 622 527 142
Fragments arranged by size:
1361 622 537 527 142
PspOMI G'GGCC_C
Cuts at: 0 292 1214 1463 1493 2509 2912 3189
Size: 292 922 249 30 1016 403 277
Fragments arranged by size:
1016 922 403 292 277 249 30
PstI C_TGCA'G
Cuts at: 0 210 1292 1396 2045 2079 2100 2668 2795
Size: 210 1082 104 649 34 21 568 127
Cuts at: 2795 2960 3189
Size: 165 229
Fragments arranged by size:
1082 649 568 229 210 165 127 104
34 21
PvuII CAG'CTG
Cuts at: 0 696 1578 1917 3105 3150 3189
Size: 696 882 339 1188 45 39
Fragments arranged by size:
1188 882 696 339 45 39
SacI G_AGCT'C
Cuts at: 0 705 1505 2405 2591 2800 3188 3189
Size: 705 800 900 186 209 388 1
Fragments arranged by size:
900 800 705 388 209 186 1
SanDI GG'GwC_CC
Cuts at: 0 537 1898 3189
Size: 537 1361 1291
Fragments arranged by size:
1361 1291 537
SapI GCTCTTCn'nnn_
Cuts at: 0 2261 2579 3189
Size: 2261 318 610
Fragments arranged by size:
2261 610 318
SexAI A'CCwGG_T
Cuts at: 0 1607 3189
Size: 1607 1582
SfcI C'TryA_G
Cuts at: 0 206 401 1288 1392 2041 2075 2096 2664
Size: 206 195 887 104 649 34 21 568
Cuts at: 2664 2791 2956 3189
Size: 127 165 233
Fragments arranged by size:
887 649 568 233 206 195 165 127
104 34 21
SfoI GGC'GCC
Cuts at: 0 2683 3189
Size: 2683 506
SmaI CCC'GGG
Cuts at: 0 1315 2713 3189
Size: 1315 1398 476
Fragments arranged by size:
1398 1315 476
SmlI C'TyrA_G
Cuts at: 0 1227 2939 3189
Size: 1227 1712 250
Fragments arranged by size:
1712 1227 250
StuI AGG'CCT
Cuts at: 0 223 231 637 2613 2830 3189
Size: 223 8 406 1976 217 359
Fragments arranged by size:
1976 406 359 223 217 8
StyI C'CwwG_G
Cuts at: 0 102 743 1376 1475 1946 2172 3189
Size: 102 641 633 99 471 226 1017
Fragments arranged by size:
1017 641 633 471 226 102 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 697 1638 1863 3189
Size: 697 941 225 1326
Fragments arranged by size:
1326 941 697 225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 47 79 114 146 718 750 1528 1560
Size: 47 32 35 32 572 32 778 32
Cuts at: 1560 3189
Size: 1629
Fragments arranged by size:
1629 778 572 47 35 32 32 32
32
XmaI C'CCGG_G
Cuts at: 0 1313 2711 3189
Size: 1313 1398 478
Fragments arranged by size:
1398 1313 478
ZraI GAC'GTC
Cuts at: 0 2457 3189
Size: 2457 732
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AgeI AleI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HincII HindIII KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NruI NspI PasI PciI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SanDI SapI SexAI
SfcI SfoI SmaI SmlI StuI StyI TaqII TstI
XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AhdI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BclI BfrBI BmgBI BmtI
BplI BsaBI BsiEI BsiWI BspEI BspHI BsrGI BssHII
BstBI BstEII BstZ17I BtgZI ClaI DraI EagI EcoRI
EcoRV FalI FseI FspAI HpaI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NsiI PacI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XcmI
XhoI XmnI