Restriction map for
pF1KSDA1895
(Linear) (Six-base) MAPSORT of: KIAA1895.seq Check: 6961 from: 1 to: 1833
KIAA1895.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1120 1833
Size: 1120 713
AccI GT'mk_AC
Cuts at: 0 1543 1833
Size: 1543 290
AclI AA'CG_TT
Cuts at: 0 997 1833
Size: 997 836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1354 1833
Size: 464 890 479
Fragments arranged by size:
890 479 464
AflII C'TTAA_G
Cuts at: 0 280 1833
Size: 280 1553
AflIII A'CryG_T
Cuts at: 0 1497 1833
Size: 1497 336
AleI CACnn'nnGTG
Cuts at: 0 868 1833
Size: 868 965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1463 1833
Size: 1463 370
ApoI r'AATT_y
Cuts at: 0 547 1017 1603 1833
Size: 547 470 586 230
Fragments arranged by size:
586 547 470 230
BbsI GAAGACnn'nnnn_
Cuts at: 0 209 1833
Size: 209 1624
BfrBI ATG'CAT
Cuts at: 0 1665 1833
Size: 1665 168
BmrI ACTGGGnnnn_n'
Cuts at: 0 815 896 1833
Size: 815 81 937
Fragments arranged by size:
937 815 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1740 1833
Size: 1740 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 724 1833
Size: 316 408 1109
Fragments arranged by size:
1109 408 316
BsaI GGTCTCn'nnnn_
Cuts at: 0 87 134 1833
Size: 87 47 1699
Fragments arranged by size:
1699 87 47
BsaWI w'CCGG_w
Cuts at: 0 78 1833
Size: 78 1755
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 152 1833
Size: 152 1681
BseYI C'CCAG_C
Cuts at: 0 758 1833
Size: 758 1075
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 845 1833
Size: 845 988
BsiHKAI G_wGCw'C
Cuts at: 0 68 1833
Size: 68 1765
BsmI GAATG_Cn'
Cuts at: 0 428 1833
Size: 428 1405
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1060 1169 1833
Size: 1060 109 664
Fragments arranged by size:
1060 664 109
Bsp1286I G_dGCh'C
Cuts at: 0 68 1833
Size: 68 1765
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1120 1833
Size: 1120 713
BstXI CCAn_nnnn'nTGG
Cuts at: 0 511 1196 1426 1833
Size: 511 685 230 407
Fragments arranged by size:
685 511 407 230
BstZ17I GTA'TAC
Cuts at: 0 1544 1833
Size: 1544 289
Bsu36I CC'TnA_GG
Cuts at: 0 1466 1833
Size: 1466 367
BtgI C'CryG_G
Cuts at: 0 1730 1771 1833
Size: 1730 41 62
Fragments arranged by size:
1730 62 41
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1098 1133 1833
Size: 1098 35 700
Fragments arranged by size:
1098 700 35
DraI TTT'AAA
Cuts at: 0 1125 1833
Size: 1125 708
DraIII CAC_nnn'GTG
Cuts at: 0 295 1396 1833
Size: 295 1101 437
Fragments arranged by size:
1101 437 295
DrdI GACnn_nn'nnGTC
Cuts at: 0 1321 1833
Size: 1321 512
EaeI y'GGCC_r
Cuts at: 0 450 1416 1658 1833
Size: 450 966 242 175
Fragments arranged by size:
966 450 242 175
EarI CTCTTCn'nnn_
Cuts at: 0 1238 1301 1833
Size: 1238 63 532
Fragments arranged by size:
1238 532 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 464 724 1354 1833
Size: 316 148 260 630 479
Fragments arranged by size:
630 479 316 260 148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 394 1833
Size: 394 1439
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 342 374 514 546 644 676 869 901
Size: 342 32 140 32 98 32 193 32
Cuts at: 901 1833
Size: 932
Fragments arranged by size:
932 342 193 140 98 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 607 1833
Size: 607 1226
MfeI C'AATT_G
Cuts at: 0 563 1833
Size: 563 1270
MscI TGG'CCA
Cuts at: 0 452 1418 1660 1833
Size: 452 966 242 173
Fragments arranged by size:
966 452 242 173
MslI CAynn'nnrTG
Cuts at: 0 868 1424 1833
Size: 868 556 409
Fragments arranged by size:
868 556 409
NcoI C'CATG_G
Cuts at: 0 1730 1771 1833
Size: 1730 41 62
Fragments arranged by size:
1730 62 41
NdeI CA'TA_TG
Cuts at: 0 1425 1538 1833
Size: 1425 113 295
Fragments arranged by size:
1425 295 113
NsiI A_TGCA'T
Cuts at: 0 1667 1833
Size: 1667 166
NspI r_CATG'y
Cuts at: 0 1156 1669 1833
Size: 1156 513 164
Fragments arranged by size:
1156 513 164
PflMI CCAn_nnn'nTGG
Cuts at: 0 1396 1833
Size: 1396 437
SapI GCTCTTCn'nnn_
Cuts at: 0 1238 1301 1833
Size: 1238 63 532
Fragments arranged by size:
1238 532 63
ScaI AGT'ACT
Cuts at: 0 111 1833
Size: 111 1722
SmlI C'TyrA_G
Cuts at: 0 280 1833
Size: 280 1553
SphI G_CATG'C
Cuts at: 0 1669 1833
Size: 1669 164
StyI C'CwwG_G
Cuts at: 0 363 417 1285 1730 1771 1833
Size: 363 54 868 445 41 62
Fragments arranged by size:
868 445 363 62 54 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 772 1833
Size: 772 1061
TatI w'GTAC_w
Cuts at: 0 109 407 1833
Size: 109 298 1426
Fragments arranged by size:
1426 298 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 658 690 1833
Size: 658 32 1143
Fragments arranged by size:
1143 658 32
XbaI T'CTAG_A
Cuts at: 0 133 1833
Size: 133 1700
XmnI GAAnn'nnTTC
Cuts at: 0 1499 1833
Size: 1499 334
Enzymes that do cut:
AarI AccI AclI AcuI AflII AflIII AleI AlfI
ApoI BbsI BfrBI BmrI BplI BpmI BsaI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BstXI BstZ17I Bsu36I BtgI CspCI DraI DraIII DrdI
EaeI EarI Eco57MI FalI Hin4I HindIII MfeI MscI
MslI NcoI NdeI NsiI NspI PflMI SapI ScaI
SmlI SphI StyI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AgeI AhdI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaXI BsiEI BsiWI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgZI BtsI ClaI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MluI MmeI MspA1I NaeI NarI
NgoMIV NheI NotI NruI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XcmI XhoI XmaI ZraI