Restriction map for
pF1KA1902
(Linear) (Six-base) MAPSORT of: KIAA1902.seq Check: 4069 from: 1 to: 3279
KIAA1902.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1025 3279
Size: 1025 2254
AccI GT'mk_AC
Cuts at: 0 1396 3279
Size: 1396 1883
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1924 2521 2800 2833 3279
Size: 1924 597 279 33 446
Fragments arranged by size:
1924 597 446 279 33
AfeI AGC'GCT
Cuts at: 0 895 3279
Size: 895 2384
AflIII A'CryG_T
Cuts at: 0 3126 3279
Size: 3126 153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2407 2846 3279
Size: 2407 439 433
Fragments arranged by size:
2407 439 433
AlwNI CAG_nnn'CTG
Cuts at: 0 1732 2394 2428 3279
Size: 1732 662 34 851
Fragments arranged by size:
1732 851 662 34
ApoI r'AATT_y
Cuts at: 0 283 332 370 630 1002 1027 1445 1955
Size: 283 49 38 260 372 25 418 510
Cuts at: 1955 1981 2462 3279
Size: 26 481 817
Fragments arranged by size:
817 510 481 418 372 283 260 49
38 26 25
AseI AT'TA_AT
Cuts at: 0 971 3279
Size: 971 2308
BamHI G'GATC_C
Cuts at: 0 261 3039 3279
Size: 261 2778 240
Fragments arranged by size:
2778 261 240
BanI G'GyrC_C
Cuts at: 0 3148 3262 3279
Size: 3148 114 17
Fragments arranged by size:
3148 114 17
BanII G_rGCy'C
Cuts at: 0 481 1140 2720 3279
Size: 481 659 1580 559
Fragments arranged by size:
1580 659 559 481
BbsI GAAGACnn'nnnn_
Cuts at: 0 585 935 1923 2185 2901 3108 3279
Size: 585 350 988 262 716 207 171
Fragments arranged by size:
988 716 585 350 262 207 171
BbvCI CC'TCA_GC
Cuts at: 0 2971 3279
Size: 2971 308
BclI T'GATC_A
Cuts at: 0 195 3237 3279
Size: 195 3042 42
Fragments arranged by size:
3042 195 42
BfrBI ATG'CAT
Cuts at: 0 2760 3279
Size: 2760 519
BglII A'GATC_T
Cuts at: 0 2547 3171 3279
Size: 2547 624 108
Fragments arranged by size:
2547 624 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 1902 3279
Size: 1902 1377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 522 1981 2488 2704 3279
Size: 112 410 1459 507 216 575
Fragments arranged by size:
1459 575 507 410 216 112
Bpu10I CC'TnA_GC
Cuts at: 0 1761 2971 3279
Size: 1761 1210 308
Fragments arranged by size:
1761 1210 308
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1322 2440 2710 3279
Size: 1322 1118 270 569
Fragments arranged by size:
1322 1118 569 270
BsaAI yAC'GTr
Cuts at: 0 685 3279
Size: 685 2594
BsaBI GATnn'nnATC
Cuts at: 0 2297 2350 3279
Size: 2297 53 929
Fragments arranged by size:
2297 929 53
BsaWI w'CCGG_w
Cuts at: 0 2927 3279
Size: 2927 352
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 580 3279
Size: 550 30 2699
Fragments arranged by size:
2699 550 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 1574 1670 1679 1682 1706 1709 1745
Size: 109 1465 96 9 3 24 3 36
Cuts at: 1745 2390 2528 3225 3279
Size: 645 138 697 54
Fragments arranged by size:
1465 697 645 138 109 96 54 36
24 9 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 572 1766 3279
Size: 508 64 1194 1513
Fragments arranged by size:
1513 1194 508 64
BsiHKAI G_wGCw'C
Cuts at: 0 481 1140 2720 3279
Size: 481 659 1580 559
Fragments arranged by size:
1580 659 559 481
BsmI GAATG_Cn'
Cuts at: 0 1170 1883 2209 2384 3279
Size: 1170 713 326 175 895
Fragments arranged by size:
1170 895 713 326 175
Bsp1286I G_dGCh'C
Cuts at: 0 481 1140 2720 3279
Size: 481 659 1580 559
Fragments arranged by size:
1580 659 559 481
BspHI T'CATG_A
Cuts at: 0 692 1416 2306 3279
Size: 692 724 890 973
Fragments arranged by size:
973 890 724 692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 530 548 1025 1519 2025 3279
Size: 530 18 477 494 506 1254
Fragments arranged by size:
1254 530 506 494 477 18
BsrBI CCG'CTC
Cuts at: 0 572 2262 3279
Size: 572 1690 1017
Fragments arranged by size:
1690 1017 572
BsrDI GCAATG_nn'
Cuts at: 0 604 3279
Size: 604 2675
BstAPI GCAn_nnn'nTGC
Cuts at: 0 54 1281 2848 3279
Size: 54 1227 1567 431
Fragments arranged by size:
1567 1227 431 54
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1260 3279
Size: 1260 2019
BstYI r'GATC_y
Cuts at: 0 261 1100 2547 3039 3171 3279
Size: 261 839 1447 492 132 108
Fragments arranged by size:
1447 839 492 261 132 108
Bsu36I CC'TnA_GG
Cuts at: 0 1566 3279
Size: 1566 1713
BtgI C'CryG_G
Cuts at: 0 1508 3279
Size: 1508 1771
BtsI GCAGTG_nn'
Cuts at: 0 589 1594 2182 3279
Size: 589 1005 588 1097
Fragments arranged by size:
1097 1005 589 588
DraI TTT'AAA
Cuts at: 0 870 1970 2532 3279
Size: 870 1100 562 747
Fragments arranged by size:
1100 870 747 562
DraIII CAC_nnn'GTG
Cuts at: 0 54 2634 3279
Size: 54 2580 645
Fragments arranged by size:
2580 645 54
EarI CTCTTCn'nnn_
Cuts at: 0 2951 3279
Size: 2951 328
EcoICRI GAG'CTC
Cuts at: 0 479 1138 2718 3279
Size: 479 659 1580 561
Fragments arranged by size:
1580 659 561 479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 522 1924 1981 2488 2521 2704 2800
Size: 112 410 1402 57 507 33 183 96
Cuts at: 2800 2833 3279
Size: 33 446
Fragments arranged by size:
1402 507 446 410 183 112 96 57
33 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 504 1176 1724 3279
Size: 504 672 548 1555
Fragments arranged by size:
1555 672 548 504
EcoO109I rG'GnC_Cy
Cuts at: 0 519 1543 1570 1729 2014 3279
Size: 519 1024 27 159 285 1265
Fragments arranged by size:
1265 1024 519 285 159 27
EcoRI G'AATT_C
Cuts at: 0 1955 3279
Size: 1955 1324
EcoRV GAT'ATC
Cuts at: 0 1470 3279
Size: 1470 1809
HaeII r_GCGC'y
Cuts at: 0 897 3231 3279
Size: 897 2334 48
Fragments arranged by size:
2334 897 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 92 124 180 212 2382 2414 2532 2551
Size: 92 32 56 32 2170 32 118 19
Cuts at: 2551 2564 2583 3031 3063 3279
Size: 13 19 448 32 216
Fragments arranged by size:
2170 448 216 118 92 56 32 32
32 32 19 19 13
HincII GTy'rAC
Cuts at: 0 2570 3279
Size: 2570 709
HindIII A'AGCT_T
Cuts at: 0 1087 3279
Size: 1087 2192
MslI CAynn'nnrTG
Cuts at: 0 721 2389 3131 3279
Size: 721 1668 742 148
Fragments arranged by size:
1668 742 721 148
MspA1I CmG'CkG
Cuts at: 0 268 1791 1840 3279
Size: 268 1523 49 1439
Fragments arranged by size:
1523 1439 268 49
NcoI C'CATG_G
Cuts at: 0 1508 3279
Size: 1508 1771
NsiI A_TGCA'T
Cuts at: 0 2762 3279
Size: 2762 517
NspI r_CATG'y
Cuts at: 0 667 2413 3130 3279
Size: 667 1746 717 149
Fragments arranged by size:
1746 717 667 149
PciI A'CATG_T
Cuts at: 0 3126 3279
Size: 3126 153
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2978 3010 3279
Size: 2978 32 269
Fragments arranged by size:
2978 269 32
PpuMI rG'GwC_Cy
Cuts at: 0 519 1543 1570 2014 3279
Size: 519 1024 27 444 1265
Fragments arranged by size:
1265 1024 519 444 27
PsiI TTA'TAA
Cuts at: 0 2648 3279
Size: 2648 631
PstI C_TGCA'G
Cuts at: 0 1022 2682 2825 3279
Size: 1022 1660 143 454
Fragments arranged by size:
1660 1022 454 143
PvuII CAG'CTG
Cuts at: 0 268 1840 3279
Size: 268 1572 1439
Fragments arranged by size:
1572 1439 268
SacI G_AGCT'C
Cuts at: 0 481 1140 2720 3279
Size: 481 659 1580 559
Fragments arranged by size:
1580 659 559 481
SanDI GG'GwC_CC
Cuts at: 0 1543 3279
Size: 1543 1736
ScaI AGT'ACT
Cuts at: 0 468 3279
Size: 468 2811
SfcI C'TryA_G
Cuts at: 0 1018 2678 2821 3279
Size: 1018 1660 143 458
Fragments arranged by size:
1660 1018 458 143
SmlI C'TyrA_G
Cuts at: 0 1337 2455 2689 3279
Size: 1337 1118 234 590
Fragments arranged by size:
1337 1118 590 234
SspI AAT'ATT
Cuts at: 0 975 1994 3279
Size: 975 1019 1285
Fragments arranged by size:
1285 1019 975
StuI AGG'CCT
Cuts at: 0 1043 3279
Size: 1043 2236
StyI C'CwwG_G
Cuts at: 0 1172 1508 2010 2484 3279
Size: 1172 336 502 474 795
Fragments arranged by size:
1172 795 502 474 336
SwaI ATTT'AAAT
Cuts at: 0 1970 3279
Size: 1970 1309
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 49 1824 3279
Size: 49 1775 1455
Fragments arranged by size:
1775 1455 49
TatI w'GTAC_w
Cuts at: 0 466 2750 3279
Size: 466 2284 529
Fragments arranged by size:
2284 529 466
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2587 2619 2620 2652 3279
Size: 2587 32 1 32 627
Fragments arranged by size:
2587 627 32 32 1
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1041 1889 3279
Size: 1041 848 1390
Fragments arranged by size:
1390 1041 848
XmnI GAAnn'nnTTC
Cuts at: 0 283 582 1382 1803 2384 2785 3279
Size: 283 299 800 421 581 401 494
Fragments arranged by size:
800 581 494 421 401 299 283
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlfI AlwNI ApoI
AseI BamHI BanI BanII BbsI BbvCI BclI BfrBI
BglII BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraI DraIII EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV HaeII Hin4I HincII HindIII MslI MspA1I NcoI
NsiI NspI PciI PpiI PpuMI PsiI PstI PvuII
SacI SanDI ScaI SfcI SmlI SspI StuI StyI
SwaI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI
BcgI BciVI BglI BlpI Bme1580I BmgBI BmtI BplI
BsaI BsaHI BseYI BsiEI BsiWI BsmBI BspEI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DrdI EaeI EagI EciI FalI FseI FspI
FspAI HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PflMI PfoI PmeI PmlI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI Tth111I XbaI XhoI XmaI ZraI