Restriction map for
pF1KSDA1904
(Linear) (Six-base) MAPSORT of: KIAA1904.seq Check: 1127 from: 1 to: 2460
KIAA1904.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1126 2460
Size: 1126 1334
AatII G_ACGT'C
Cuts at: 0 1007 2460
Size: 1007 1453
AfeI AGC'GCT
Cuts at: 0 2314 2460
Size: 2314 146
AflIII A'CryG_T
Cuts at: 0 98 2460
Size: 98 2362
AleI CACnn'nnGTG
Cuts at: 0 931 2460
Size: 931 1529
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 256 288 2460
Size: 256 32 2172
Fragments arranged by size:
2172 256 32
AlwNI CAG_nnn'CTG
Cuts at: 0 321 1374 1753 1771 2457 2460
Size: 321 1053 379 18 686 3
Fragments arranged by size:
1053 686 379 321 18 3
ApaI G_GGCC'C
Cuts at: 0 1785 2460
Size: 1785 675
ApaLI G'TGCA_C
Cuts at: 0 169 1357 2460
Size: 169 1188 1103
Fragments arranged by size:
1188 1103 169
AvaI C'yCGr_G
Cuts at: 0 450 816 1549 1570 1714 1777 2275 2460
Size: 450 366 733 21 144 63 498 185
Fragments arranged by size:
733 498 450 366 185 144 63 21
BanI G'GyrC_C
Cuts at: 0 52 289 502 681 1161 1522 2037 2158
Size: 52 237 213 179 480 361 515 121
Cuts at: 2158 2460
Size: 302
Fragments arranged by size:
515 480 361 302 237 213 179 121
52
BanII G_rGCy'C
Cuts at: 0 1588 1785 1828 2460
Size: 1588 197 43 632
Fragments arranged by size:
1588 632 197 43
BbeI G_GCGC'C
Cuts at: 0 1165 1526 2460
Size: 1165 361 934
Fragments arranged by size:
1165 934 361
BbsI GAAGACnn'nnnn_
Cuts at: 0 597 1312 2460
Size: 597 715 1148
Fragments arranged by size:
1148 715 597
BbvCI CC'TCA_GC
Cuts at: 0 487 1764 2460
Size: 487 1277 696
Fragments arranged by size:
1277 696 487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 271 305 1209 1243 1488 1522 2140 2174
Size: 271 34 904 34 245 34 618 34
Cuts at: 2174 2460
Size: 286
Fragments arranged by size:
904 618 286 271 245 34 34 34
34
BglI GCCn_nnn'nGGC
Cuts at: 0 756 1217 1864 2460
Size: 756 461 647 596
Fragments arranged by size:
756 647 596 461
BglII A'GATC_T
Cuts at: 0 269 1598 2303 2460
Size: 269 1329 705 157
Fragments arranged by size:
1329 705 269 157
BlpI GC'TnA_GC
Cuts at: 0 343 1852 2460
Size: 343 1509 608
Fragments arranged by size:
1509 608 343
Bme1580I G_kGCm'C
Cuts at: 0 173 451 686 1361 1785 1943 2460
Size: 173 278 235 675 424 158 517
Fragments arranged by size:
675 517 424 278 235 173 158
BmgBI CAC'GTC
Cuts at: 0 909 1938 2460
Size: 909 1029 522
Fragments arranged by size:
1029 909 522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1336 1807 2110 2253 2460
Size: 706 630 471 303 143 207
Fragments arranged by size:
706 630 471 303 207 143
Bpu10I CC'TnA_GC
Cuts at: 0 487 1764 1970 2170 2460
Size: 487 1277 206 200 290
Fragments arranged by size:
1277 487 290 206 200
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1648 2176 2460
Size: 1000 648 528 284
Fragments arranged by size:
1000 648 528 284
BsaI GGTCTCn'nnnn_
Cuts at: 0 133 2445 2460
Size: 133 2312 15
Fragments arranged by size:
2312 133 15
BsaAI yAC'GTr
Cuts at: 0 99 2460
Size: 99 2361
BsaBI GATnn'nnATC
Cuts at: 0 967 2460
Size: 967 1493
BsaHI Gr'CG_yC
Cuts at: 0 427 791 1004 1162 1523 2460
Size: 427 364 213 158 361 937
Fragments arranged by size:
937 427 364 361 213 158
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 256 286 460 490 2335 2365 2460
Size: 256 30 174 30 1845 30 95
Fragments arranged by size:
1845 256 174 95 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 207 1291 1752 2167 2256 2359 2460
Size: 207 1084 461 415 89 103 101
Fragments arranged by size:
1084 461 415 207 103 101 89
BseYI C'CCAG_C
Cuts at: 0 327 1165 1233 1374 1795 2447 2460
Size: 327 838 68 141 421 652 13
Fragments arranged by size:
838 652 421 327 141 68 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 997 2460
Size: 886 111 1463
Fragments arranged by size:
1463 886 111
BsiEI CG_ry'CG
Cuts at: 0 49 633 1868 1955 2460
Size: 49 584 1235 87 505
Fragments arranged by size:
1235 584 505 87 49
BsiHKAI G_wGCw'C
Cuts at: 0 173 212 1361 1828 2123 2296 2460
Size: 173 39 1149 467 295 173 164
Fragments arranged by size:
1149 467 295 173 173 164 39
Bsp1286I G_dGCh'C
Cuts at: 0 173 212 451 686 1361 1588 1785 1828
Size: 173 39 239 235 675 227 197 43
Cuts at: 1828 1943 2123 2296 2460
Size: 115 180 173 164
Fragments arranged by size:
675 239 235 227 197 180 173 173
164 115 43 39
BspHI T'CATG_A
Cuts at: 0 1007 1193 2460
Size: 1007 186 1267
Fragments arranged by size:
1267 1007 186
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 185 306 1126 1891 2460
Size: 185 121 820 765 569
Fragments arranged by size:
820 765 569 185 121
BsrBI CCG'CTC
Cuts at: 0 1791 2207 2460
Size: 1791 416 253
Fragments arranged by size:
1791 416 253
BsrDI GCAATG_nn'
Cuts at: 0 1607 2460
Size: 1607 853
BsrFI r'CCGG_y
Cuts at: 0 44 547 664 855 1345 1525 2024 2040
Size: 44 503 117 191 490 180 499 16
Cuts at: 2040 2100 2123 2362 2460
Size: 60 23 239 98
Fragments arranged by size:
503 499 490 239 191 180 117 98
60 44 23 16
BsrGI T'GTAC_A
Cuts at: 0 965 2351 2460
Size: 965 1386 109
Fragments arranged by size:
1386 965 109
BssSI C'ACGA_G
Cuts at: 0 973 2460
Size: 973 1487
BstAPI GCAn_nnn'nTGC
Cuts at: 0 177 321 1753 1883 2460
Size: 177 144 1432 130 577
Fragments arranged by size:
1432 577 177 144 130
BstXI CCAn_nnnn'nTGG
Cuts at: 0 531 933 1458 1620 1965 2460
Size: 531 402 525 162 345 495
Fragments arranged by size:
531 525 495 402 345 162
BstYI r'GATC_y
Cuts at: 0 269 836 1598 2303 2406 2460
Size: 269 567 762 705 103 54
Fragments arranged by size:
762 705 567 269 103 54
BtgI C'CryG_G
Cuts at: 0 652 869 2460
Size: 652 217 1591
Fragments arranged by size:
1591 652 217
BtsI GCAGTG_nn'
Cuts at: 0 646 1710 2109 2460
Size: 646 1064 399 351
Fragments arranged by size:
1064 646 399 351
ClaI AT'CG_AT
Cuts at: 0 1655 2460
Size: 1655 805
EaeI y'GGCC_r
Cuts at: 0 46 107 545 923 1578 1865 1952 2357
Size: 46 61 438 378 655 287 87 405
Cuts at: 2357 2460
Size: 103
Fragments arranged by size:
655 438 405 378 287 103 87 61
46
EagI C'GGCC_G
Cuts at: 0 46 1865 1952 2460
Size: 46 1819 87 508
Fragments arranged by size:
1819 508 87 46
EarI CTCTTCn'nnn_
Cuts at: 0 583 2460
Size: 583 1877
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2433 2460
Size: 2433 27
EcoICRI GAG'CTC
Cuts at: 0 1826 2460
Size: 1826 634
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1336 1807 2110 2253 2460
Size: 706 630 471 303 143 207
Fragments arranged by size:
706 630 471 303 207 143
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 415 2460
Size: 355 60 2045
Fragments arranged by size:
2045 355 60
EcoO109I rG'GnC_Cy
Cuts at: 0 318 1140 1710 1781 1782 2460
Size: 318 822 570 71 1 678
Fragments arranged by size:
822 678 570 318 71 1
FspI TGC'GCA
Cuts at: 0 1093 1258 1516 2377 2460
Size: 1093 165 258 861 83
Fragments arranged by size:
1093 861 258 165 83
FspAI rTGC'GCAy
Cuts at: 0 1516 2460
Size: 1516 944
HaeII r_GCGC'y
Cuts at: 0 31 1165 1526 1848 1859 1919 2316 2460
Size: 31 1134 361 322 11 60 397 144
Fragments arranged by size:
1134 397 361 322 144 60 31 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 460 492 799 831 1333 1365 1663 1695
Size: 460 32 307 32 502 32 298 32
Cuts at: 1695 1720 1752 2269 2301 2460
Size: 25 32 517 32 159
Fragments arranged by size:
517 502 460 307 298 159 32 32
32 32 32 32 25
HincII GTy'rAC
Cuts at: 0 1296 1632 2460
Size: 1296 336 828
Fragments arranged by size:
1296 828 336
KasI G'GCGC_C
Cuts at: 0 1161 1522 2460
Size: 1161 361 938
Fragments arranged by size:
1161 938 361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 715 1025 1439 2460
Size: 503 212 310 414 1021
Fragments arranged by size:
1021 503 414 310 212
MscI TGG'CCA
Cuts at: 0 109 2460
Size: 109 2351
MslI CAynn'nnrTG
Cuts at: 0 418 931 973 2460
Size: 418 513 42 1487
Fragments arranged by size:
1487 513 418 42
MspA1I CmG'CkG
Cuts at: 0 327 654 675 1750 1851 2019 2034 2051
Size: 327 327 21 1075 101 168 15 17
Cuts at: 2051 2063 2199 2460
Size: 12 136 261
Fragments arranged by size:
1075 327 327 261 168 136 101 21
17 15 12
NaeI GCC'GGC
Cuts at: 0 46 549 666 857 1347 1527 2042 2102
Size: 46 503 117 191 490 180 515 60
Cuts at: 2102 2460
Size: 358
Fragments arranged by size:
515 503 490 358 191 180 117 60
46
NarI GG'CG_CC
Cuts at: 0 1162 1523 2460
Size: 1162 361 937
Fragments arranged by size:
1162 937 361
NgoMIV G'CCGG_C
Cuts at: 0 44 547 664 855 1345 1525 2040 2100
Size: 44 503 117 191 490 180 515 60
Cuts at: 2100 2460
Size: 360
Fragments arranged by size:
515 503 490 360 191 180 117 60
44
NspI r_CATG'y
Cuts at: 0 366 1272 2460
Size: 366 906 1188
Fragments arranged by size:
1188 906 366
PflMI CCAn_nnn'nTGG
Cuts at: 0 1374 2088 2460
Size: 1374 714 372
Fragments arranged by size:
1374 714 372
PfoI T'CCnGG_A
Cuts at: 0 1313 1562 2460
Size: 1313 249 898
Fragments arranged by size:
1313 898 249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 2460
Size: 256 32 2172
Fragments arranged by size:
2172 256 32
PpuMI rG'GwC_Cy
Cuts at: 0 318 1140 1710 2460
Size: 318 822 570 750
Fragments arranged by size:
822 750 570 318
PspOMI G'GGCC_C
Cuts at: 0 1781 2460
Size: 1781 679
PstI C_TGCA'G
Cuts at: 0 118 317 326 389 641 1596 1749 1888
Size: 118 199 9 63 252 955 153 139
Cuts at: 1888 2116 2460
Size: 228 344
Fragments arranged by size:
955 344 252 228 199 153 139 118
63 9
PvuII CAG'CTG
Cuts at: 0 327 1750 1851 2460
Size: 327 1423 101 609
Fragments arranged by size:
1423 609 327 101
SacI G_AGCT'C
Cuts at: 0 1828 2460
Size: 1828 632
SacII CC_GC'GG
Cuts at: 0 655 2460
Size: 655 1805
SanDI GG'GwC_CC
Cuts at: 0 1140 1710 2460
Size: 1140 570 750
Fragments arranged by size:
1140 750 570
SbfI CC_TGCA'GG
Cuts at: 0 317 2460
Size: 317 2143
ScaI AGT'ACT
Cuts at: 0 2281 2460
Size: 2281 179
SfcI C'TryA_G
Cuts at: 0 114 313 322 385 637 954 1592 1745
Size: 114 199 9 63 252 317 638 153
Cuts at: 1745 1840 1884 2112 2460
Size: 95 44 228 348
Fragments arranged by size:
638 348 317 252 228 199 153 114
95 63 44 9
SfoI GGC'GCC
Cuts at: 0 1163 1524 2460
Size: 1163 361 936
Fragments arranged by size:
1163 936 361
SgrAI Cr'CCGG_yG
Cuts at: 0 1525 2460
Size: 1525 935
SmaI CCC'GGG
Cuts at: 0 1779 2460
Size: 1779 681
SmlI C'TyrA_G
Cuts at: 0 1015 1570 1663 2191 2460
Size: 1015 555 93 528 269
Fragments arranged by size:
1015 555 528 269 93
SphI G_CATG'C
Cuts at: 0 366 1272 2460
Size: 366 906 1188
Fragments arranged by size:
1188 906 366
StyI C'CwwG_G
Cuts at: 0 1451 1481 1613 1919 2396 2460
Size: 1451 30 132 306 477 64
Fragments arranged by size:
1451 477 306 132 64 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1198 1494 1850 2460
Size: 1198 296 356 610
Fragments arranged by size:
1198 610 356 296
TatI w'GTAC_w
Cuts at: 0 965 980 1067 1244 1985 2144 2279 2351
Size: 965 15 87 177 741 159 135 72
Cuts at: 2351 2460
Size: 109
Fragments arranged by size:
965 741 177 159 135 109 87 72
15
Tth111I GACn'n_nGTC
Cuts at: 0 1144 1627 2460
Size: 1144 483 833
Fragments arranged by size:
1144 833 483
XhoI C'TCGA_G
Cuts at: 0 1570 2460
Size: 1570 890
XmaI C'CCGG_G
Cuts at: 0 1777 2460
Size: 1777 683
ZraI GAC'GTC
Cuts at: 0 1005 2460
Size: 1005 1455
Enzymes that do cut:
AarI AatII AfeI AflIII AleI AloI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstXI BstYI BtgI BtsI ClaI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FspI FspAI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PflMI PfoI
PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI
SbfI ScaI SfcI SfoI SgrAI SmaI SmlI SphI
StyI TaqII TatI Tth111I XhoI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AflII AgeI AhdI AlfI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BfrBI BmrI BmtI BplI BsaWI BsiWI BsmI
BsmBI BspEI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI
CspCI DraI DraIII DrdI EcoRI EcoRV FalI FseI
HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI
SexAI SfiI SgfI SnaBI SpeI SrfI SspI StuI
SwaI TstI XbaI XcmI XmnI