Restriction map for
pF1KA1910
(Linear) (Six-base) MAPSORT of: KIAA1910.seq Check: 122 from: 1 to: 2088
KIAA1910.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AatII G_ACGT'C
Cuts at: 0 31 2088
Size: 31 2057
AccI GT'mk_AC
Cuts at: 0 119 2088
Size: 119 1969
AclI AA'CG_TT
Cuts at: 0 53 2088
Size: 53 2035
AflIII A'CryG_T
Cuts at: 0 114 515 1466 2088
Size: 114 401 951 622
Fragments arranged by size:
951 622 401 114
AhdI GACnn_n'nnGTC
Cuts at: 0 1311 2088
Size: 1311 777
AleI CACnn'nnGTG
Cuts at: 0 1609 1903 2088
Size: 1609 294 185
Fragments arranged by size:
1609 294 185
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1627 2088
Size: 1627 461
AlwNI CAG_nnn'CTG
Cuts at: 0 907 2088
Size: 907 1181
ApaI G_GGCC'C
Cuts at: 0 2049 2088
Size: 2049 39
ApoI r'AATT_y
Cuts at: 0 1324 2088
Size: 1324 764
AseI AT'TA_AT
Cuts at: 0 1982 2088
Size: 1982 106
AvaI C'yCGr_G
Cuts at: 0 438 1316 1389 1863 2088
Size: 438 878 73 474 225
Fragments arranged by size:
878 474 438 225 73
BamHI G'GATC_C
Cuts at: 0 1421 2088
Size: 1421 667
BanI G'GyrC_C
Cuts at: 0 816 2088
Size: 816 1272
BanII G_rGCy'C
Cuts at: 0 2049 2088
Size: 2049 39
BbeI G_GCGC'C
Cuts at: 0 820 2088
Size: 820 1268
BbvCI CC'TCA_GC
Cuts at: 0 1447 1736 2088
Size: 1447 289 352
Fragments arranged by size:
1447 352 289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 701 735 2088
Size: 701 34 1353
Fragments arranged by size:
1353 701 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1028 2088
Size: 1028 1060
BglII A'GATC_T
Cuts at: 0 80 1727 2088
Size: 80 1647 361
Fragments arranged by size:
1647 361 80
Bme1580I G_kGCm'C
Cuts at: 0 533 1396 2049 2088
Size: 533 863 653 39
Fragments arranged by size:
863 653 533 39
BmgBI CAC'GTC
Cuts at: 0 1467 2088
Size: 1467 621
BmrI ACTGGGnnnn_n'
Cuts at: 0 168 1799 2088
Size: 168 1631 289
Fragments arranged by size:
1631 289 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 388 487 972 2088
Size: 43 345 99 485 1116
Fragments arranged by size:
1116 485 345 99 43
Bpu10I CC'TnA_GC
Cuts at: 0 1002 1447 1736 2088
Size: 1002 445 289 352
Fragments arranged by size:
1002 445 352 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1651 2088
Size: 1651 437
BsaI GGTCTCn'nnnn_
Cuts at: 0 829 1485 1795 2088
Size: 829 656 310 293
Fragments arranged by size:
829 656 310 293
BsaAI yAC'GTr
Cuts at: 0 117 2088
Size: 117 1971
BsaBI GATnn'nnATC
Cuts at: 0 952 2088
Size: 952 1136
BsaHI Gr'CG_yC
Cuts at: 0 28 817 1678 2088
Size: 28 789 861 410
Fragments arranged by size:
861 789 410 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 828 858 1804 1834 2088
Size: 828 30 946 30 254
Fragments arranged by size:
946 828 254 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 598 1702 1956 2088
Size: 598 1104 254 132
Fragments arranged by size:
1104 598 254 132
BseYI C'CCAG_C
Cuts at: 0 1474 2088
Size: 1474 614
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 630 1150 1486 1587 2088
Size: 316 314 520 336 101 501
Fragments arranged by size:
520 501 336 316 314 101
BsiEI CG_ry'CG
Cuts at: 0 711 2088
Size: 711 1377
BsiHKAI G_wGCw'C
Cuts at: 0 476 1600 2088
Size: 476 1124 488
Fragments arranged by size:
1124 488 476
BsmI GAATG_Cn'
Cuts at: 0 702 2088
Size: 702 1386
BsmBI CGTCTCn'nnnn_
Cuts at: 0 19 35 1669 1944 2088
Size: 19 16 1634 275 144
Fragments arranged by size:
1634 275 144 19 16
Bsp1286I G_dGCh'C
Cuts at: 0 476 533 1396 1600 2049 2088
Size: 476 57 863 204 449 39
Fragments arranged by size:
863 476 449 204 57 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 516 1451 2088
Size: 516 935 637
Fragments arranged by size:
935 637 516
BsrDI GCAATG_nn'
Cuts at: 0 99 2088
Size: 99 1989
BsrFI r'CCGG_y
Cuts at: 0 1680 2088
Size: 1680 408
BssSI C'ACGA_G
Cuts at: 0 1915 2088
Size: 1915 173
BstBI TT'CG_AA
Cuts at: 0 363 2088
Size: 363 1725
BstXI CCAn_nnnn'nTGG
Cuts at: 0 941 2088
Size: 941 1147
BstYI r'GATC_y
Cuts at: 0 80 617 929 1157 1212 1421 1727 1751
Size: 80 537 312 228 55 209 306 24
Cuts at: 1751 2088
Size: 337
Fragments arranged by size:
537 337 312 306 228 209 80 55
24
Bsu36I CC'TnA_GG
Cuts at: 0 1273 1927 2088
Size: 1273 654 161
Fragments arranged by size:
1273 654 161
BtgI C'CryG_G
Cuts at: 0 1578 1901 2088
Size: 1578 323 187
Fragments arranged by size:
1578 323 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1215 2088
Size: 1215 873
BtsI GCAGTG_nn'
Cuts at: 0 158 1630 2088
Size: 158 1472 458
Fragments arranged by size:
1472 458 158
DraI TTT'AAA
Cuts at: 0 482 1064 2088
Size: 482 582 1024
Fragments arranged by size:
1024 582 482
DraIII CAC_nnn'GTG
Cuts at: 0 1185 2055 2088
Size: 1185 870 33
Fragments arranged by size:
1185 870 33
EaeI y'GGCC_r
Cuts at: 0 708 2088
Size: 708 1380
EagI C'GGCC_G
Cuts at: 0 708 2088
Size: 708 1380
EarI CTCTTCn'nnn_
Cuts at: 0 827 2013 2088
Size: 827 1186 75
Fragments arranged by size:
1186 827 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1813 1881 2088
Size: 1813 68 207
Fragments arranged by size:
1813 207 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 388 487 972 2088
Size: 43 345 99 485 1116
Fragments arranged by size:
1116 485 345 99 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 625 2088
Size: 625 1463
EcoO109I rG'GnC_Cy
Cuts at: 0 108 443 1451 2045 2088
Size: 108 335 1008 594 43
Fragments arranged by size:
1008 594 335 108 43
HaeII r_GCGC'y
Cuts at: 0 820 2088
Size: 820 1268
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 646 678 828 860 1297 1329 1719 1751
Size: 646 32 150 32 437 32 390 32
Cuts at: 1751 1948 1980 2053 2085 2088
Size: 197 32 73 32 3
Fragments arranged by size:
646 437 390 197 150 73 32 32
32 32 32 32 3
HincII GTy'rAC
Cuts at: 0 1553 1766 2088
Size: 1553 213 322
Fragments arranged by size:
1553 322 213
HpaI GTT'AAC
Cuts at: 0 1553 1766 2088
Size: 1553 213 322
Fragments arranged by size:
1553 322 213
KasI G'GCGC_C
Cuts at: 0 816 2088
Size: 816 1272
MfeI C'AATT_G
Cuts at: 0 1607 2088
Size: 1607 481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1190 1841 2088
Size: 1190 651 247
Fragments arranged by size:
1190 651 247
MslI CAynn'nnrTG
Cuts at: 0 1609 1852 1903 1915 2088
Size: 1609 243 51 12 173
Fragments arranged by size:
1609 243 173 51 12
MspA1I CmG'CkG
Cuts at: 0 46 318 1037 1740 2088
Size: 46 272 719 703 348
Fragments arranged by size:
719 703 348 272 46
NarI GG'CG_CC
Cuts at: 0 817 2088
Size: 817 1271
NspI r_CATG'y
Cuts at: 0 1914 2088
Size: 1914 174
PasI CC'CwG_GG
Cuts at: 0 1024 1051 1589 2088
Size: 1024 27 538 499
Fragments arranged by size:
1024 538 499 27
PfoI T'CCnGG_A
Cuts at: 0 449 846 988 2088
Size: 449 397 142 1100
Fragments arranged by size:
1100 449 397 142
PpuMI rG'GwC_Cy
Cuts at: 0 108 1451 2088
Size: 108 1343 637
Fragments arranged by size:
1343 637 108
PshAI GACnn'nnGTC
Cuts at: 0 779 2088
Size: 779 1309
PsiI TTA'TAA
Cuts at: 0 245 2088
Size: 245 1843
PspOMI G'GGCC_C
Cuts at: 0 2045 2088
Size: 2045 43
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1348 1380 2088
Size: 1348 32 708
Fragments arranged by size:
1348 708 32
PstI C_TGCA'G
Cuts at: 0 152 317 743 1036 2088
Size: 152 165 426 293 1052
Fragments arranged by size:
1052 426 293 165 152
PvuII CAG'CTG
Cuts at: 0 318 1037 1740 2088
Size: 318 719 703 348
Fragments arranged by size:
719 703 348 318
SfcI C'TryA_G
Cuts at: 0 148 313 739 1032 1238 1990 2088
Size: 148 165 426 293 206 752 98
Fragments arranged by size:
752 426 293 206 165 148 98
SfoI GGC'GCC
Cuts at: 0 818 2088
Size: 818 1270
SgrAI Cr'CCGG_yG
Cuts at: 0 1680 2088
Size: 1680 408
SmaI CCC'GGG
Cuts at: 0 440 1318 1391 1865 2088
Size: 440 878 73 474 223
Fragments arranged by size:
878 474 440 223 73
SmlI C'TyrA_G
Cuts at: 0 1666 2088
Size: 1666 422
SphI G_CATG'C
Cuts at: 0 1914 2088
Size: 1914 174
StyI C'CwwG_G
Cuts at: 0 637 2088
Size: 637 1451
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 553 2088
Size: 553 1535
TatI w'GTAC_w
Cuts at: 0 1364 2088
Size: 1364 724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1261 1293 1804 1836 2088
Size: 1261 32 511 32 252
Fragments arranged by size:
1261 511 252 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1854 2088
Size: 1854 234
XmaI C'CCGG_G
Cuts at: 0 438 1316 1389 1863 2088
Size: 438 878 73 474 225
Fragments arranged by size:
878 474 438 225 73
XmnI GAAnn'nnTTC
Cuts at: 0 290 686 2088
Size: 290 396 1402
Fragments arranged by size:
1402 396 290
ZraI GAC'GTC
Cuts at: 0 29 2088
Size: 29 2059
Enzymes that do cut:
AatII AccI AclI AflIII AhdI AleI AlfI AlwNI
ApaI ApoI AseI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrDI BsrFI BssSI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HpaI KasI
MfeI MmeI MslI MspA1I NarI NspI PasI PfoI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuII SfcI
SfoI SgrAI SmaI SmlI SphI StyI TaqII TatI
TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AcuI AfeI AflII AgeI AloI ApaLI
AscI AsiSI AvrII BaeI BbsI BciVI BclI BfrBI
BlpI BmtI BplI BsaWI BsiWI BspEI BspHI BsrBI
BsrGI BssHII BstAPI BstEII BstZ17I ClaI CspCI DrdI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII
KpnI MluI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PspXI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XhoI