Restriction map for
pF1KSDA1912
(Linear) (Six-base) MAPSORT of: KIAA1912.seq Check: 6388 from: 1 to: 1659
KIAA1912.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 716 1659
Size: 716 943
AccI GT'mk_AC
Cuts at: 0 1223 1659
Size: 1223 436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1500 1659
Size: 1000 500 159
Fragments arranged by size:
1000 500 159
AflII C'TTAA_G
Cuts at: 0 1567 1659
Size: 1567 92
AgeI A'CCGG_T
Cuts at: 0 959 1659
Size: 959 700
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1476 1508 1659
Size: 1476 32 151
Fragments arranged by size:
1476 151 32
AlwNI CAG_nnn'CTG
Cuts at: 0 312 384 441 551 791 1477 1659
Size: 312 72 57 110 240 686 182
Fragments arranged by size:
686 312 240 182 110 72 57
AscI GG'CGCG_CC
Cuts at: 0 171 1659
Size: 171 1488
AvaI C'yCGr_G
Cuts at: 0 368 766 1449 1659
Size: 368 398 683 210
Fragments arranged by size:
683 398 368 210
BanI G'GyrC_C
Cuts at: 0 881 1066 1171 1415 1659
Size: 881 185 105 244 244
Fragments arranged by size:
881 244 244 185 105
BanII G_rGCy'C
Cuts at: 0 494 503 940 1006 1108 1389 1659
Size: 494 9 437 66 102 281 270
Fragments arranged by size:
494 437 281 270 102 66 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 305 1659
Size: 305 1354
BbvCI CC'TCA_GC
Cuts at: 0 57 1659
Size: 57 1602
BclI T'GATC_A
Cuts at: 0 1110 1659
Size: 1110 549
Bme1580I G_kGCm'C
Cuts at: 0 1069 1174 1659
Size: 1069 105 485
Fragments arranged by size:
1069 485 105
BmrI ACTGGGnnnn_n'
Cuts at: 0 394 1659
Size: 394 1265
BmtI G_CTAG'C
Cuts at: 0 1514 1659
Size: 1514 145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 383 1659
Size: 383 1276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 472 624 1288 1309 1659
Size: 277 195 152 664 21 350
Fragments arranged by size:
664 350 277 195 152 21
Bpu10I CC'TnA_GC
Cuts at: 0 57 1659
Size: 57 1602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 478 1659
Size: 256 222 1181
Fragments arranged by size:
1181 256 222
BsaAI yAC'GTr
Cuts at: 0 609 924 1659
Size: 609 315 735
Fragments arranged by size:
735 609 315
BsaWI w'CCGG_w
Cuts at: 0 959 1214 1659
Size: 959 255 445
Fragments arranged by size:
959 445 255
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 1186 1216 1476 1506 1659
Size: 91 30 1065 30 260 30 153
Fragments arranged by size:
1065 260 153 91 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 139 163 535 1139 1659
Size: 139 24 372 604 520
Fragments arranged by size:
604 520 372 139 24
BseYI C'CCAG_C
Cuts at: 0 1373 1538 1659
Size: 1373 165 121
Fragments arranged by size:
1373 165 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 551 1659
Size: 232 319 1108
Fragments arranged by size:
1108 319 232
BsiEI CG_ry'CG
Cuts at: 0 14 68 1659
Size: 14 54 1591
Fragments arranged by size:
1591 54 14
BsiHKAI G_wGCw'C
Cuts at: 0 494 503 707 743 842 1082 1659
Size: 494 9 204 36 99 240 577
Fragments arranged by size:
577 494 240 204 99 36 9
Bsp1286I G_dGCh'C
Cuts at: 0 494 503 707 743 842 940 1006 1069
Size: 494 9 204 36 99 98 66 63
Cuts at: 1069 1082 1108 1174 1389 1659
Size: 13 26 66 215 270
Fragments arranged by size:
494 270 215 204 99 98 66 66
63 36 26 13 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 440 716 1591 1659
Size: 440 276 875 68
Fragments arranged by size:
875 440 276 68
BsrFI r'CCGG_y
Cuts at: 0 67 93 344 959 1659
Size: 67 26 251 615 700
Fragments arranged by size:
700 615 251 67 26
BssHII G'CGCG_C
Cuts at: 0 171 1659
Size: 171 1488
BstAPI GCAn_nnn'nTGC
Cuts at: 0 551 1659
Size: 551 1108
BstEII G'GTnAC_C
Cuts at: 0 1202 1659
Size: 1202 457
BstYI r'GATC_y
Cuts at: 0 260 1659
Size: 260 1399
BtgI C'CryG_G
Cuts at: 0 77 264 1659
Size: 77 187 1395
Fragments arranged by size:
1395 187 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 211 1659
Size: 211 1448
BtsI GCAGTG_nn'
Cuts at: 0 142 395 696 1137 1408 1659
Size: 142 253 301 441 271 251
Fragments arranged by size:
441 301 271 253 251 142
EaeI y'GGCC_r
Cuts at: 0 11 65 1659
Size: 11 54 1594
Fragments arranged by size:
1594 54 11
EagI C'GGCC_G
Cuts at: 0 11 65 1659
Size: 11 54 1594
Fragments arranged by size:
1594 54 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 58 196 1169 1659
Size: 58 138 973 490
Fragments arranged by size:
973 490 138 58
EcoICRI GAG'CTC
Cuts at: 0 492 501 1659
Size: 492 9 1158
Fragments arranged by size:
1158 492 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 472 624 1000 1288 1309 1500 1659
Size: 277 195 152 376 288 21 191 159
Fragments arranged by size:
376 288 277 195 191 159 152 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1257 1659
Size: 1257 402
EcoO109I rG'GnC_Cy
Cuts at: 0 102 318 826 1659
Size: 102 216 508 833
Fragments arranged by size:
833 508 216 102
EcoRV GAT'ATC
Cuts at: 0 279 1659
Size: 279 1380
FspI TGC'GCA
Cuts at: 0 540 1659
Size: 540 1119
HaeII r_GCGC'y
Cuts at: 0 761 1659
Size: 761 898
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 372 404 1095 1127 1186 1218
Size: 91 32 249 32 691 32 59 32
Cuts at: 1218 1659
Size: 441
Fragments arranged by size:
691 441 249 91 59 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 1366 1659
Size: 98 1268 293
Fragments arranged by size:
1268 293 98
MslI CAynn'nnrTG
Cuts at: 0 1217 1659
Size: 1217 442
MspA1I CmG'CkG
Cuts at: 0 79 246 266 551 1151 1266 1287 1659
Size: 79 167 20 285 600 115 21 372
Fragments arranged by size:
600 372 285 167 115 79 21 20
NaeI GCC'GGC
Cuts at: 0 69 1659
Size: 69 1590
NgoMIV G'CCGG_C
Cuts at: 0 67 1659
Size: 67 1592
NheI G'CTAG_C
Cuts at: 0 1510 1659
Size: 1510 149
NspI r_CATG'y
Cuts at: 0 1308 1528 1659
Size: 1308 220 131
Fragments arranged by size:
1308 220 131
PfoI T'CCnGG_A
Cuts at: 0 293 332 1611 1659
Size: 293 39 1279 48
Fragments arranged by size:
1279 293 48 39
PmlI CAC'GTG
Cuts at: 0 924 1659
Size: 924 735
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1476 1508 1659
Size: 1476 32 151
Fragments arranged by size:
1476 151 32
PpuMI rG'GwC_Cy
Cuts at: 0 102 318 826 1659
Size: 102 216 508 833
Fragments arranged by size:
833 508 216 102
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1541 1573 1659
Size: 1541 32 86
Fragments arranged by size:
1541 86 32
PstI C_TGCA'G
Cuts at: 0 137 245 437 713 1509 1602 1659
Size: 137 108 192 276 796 93 57
Fragments arranged by size:
796 276 192 137 108 93 57
PvuII CAG'CTG
Cuts at: 0 246 1266 1287 1659
Size: 246 1020 21 372
Fragments arranged by size:
1020 372 246 21
SacI G_AGCT'C
Cuts at: 0 494 503 1659
Size: 494 9 1156
Fragments arranged by size:
1156 494 9
SacII CC_GC'GG
Cuts at: 0 80 267 1659
Size: 80 187 1392
Fragments arranged by size:
1392 187 80
SfcI C'TryA_G
Cuts at: 0 133 241 433 709 1224 1505 1598 1659
Size: 133 108 192 276 515 281 93 61
Fragments arranged by size:
515 281 276 192 133 108 93 61
SgrAI Cr'CCGG_yG
Cuts at: 0 93 1659
Size: 93 1566
SmaI CCC'GGG
Cuts at: 0 1451 1659
Size: 1451 208
SmlI C'TyrA_G
Cuts at: 0 271 493 1567 1659
Size: 271 222 1074 92
Fragments arranged by size:
1074 271 222 92
SphI G_CATG'C
Cuts at: 0 1308 1659
Size: 1308 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 1512 1659
Size: 610 902 147
Fragments arranged by size:
902 610 147
TatI w'GTAC_w
Cuts at: 0 1643 1659
Size: 1643 16
Tth111I GACn'n_nGTC
Cuts at: 0 386 1659
Size: 386 1273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 688 1659
Size: 688 971
XmaI C'CCGG_G
Cuts at: 0 1449 1659
Size: 1449 210
Enzymes that do cut:
AarI AccI AcuI AflII AgeI AloI AlwNI AscI
AvaI BanI BanII BbsI BbvCI BclI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI
BssHII BstAPI BstEII BstYI BtgI BtgZI BtsI EaeI
EagI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI
HaeII Hin4I MmeI MslI MspA1I NaeI NgoMIV NheI
NspI PfoI PmlI PpiI PpuMI PsrI PstI PvuII
SacI SacII SfcI SgrAI SmaI SmlI SphI TaqII
TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AhdI AleI AlfI
ApaI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BfrBI BglI BglII BlpI BmgBI
BsaI BsaBI BsaHI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssSI BstBI BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EarI EcoRI FalI
FseI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NarI NcoI NdeI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PshAI PsiI PspOMI
PspXI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SnaBI SpeI SrfI SspI
StuI StyI SwaI TstI XbaI XhoI XmnI ZraI