Restriction map for
pF1KSDA1917
(Linear) (Six-base) MAPSORT of: KIAA1917.seq Check: 1084 from: 1 to: 2037
KIAA1917.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1226 2037
Size: 1226 811
AccI GT'mk_AC
Cuts at: 0 722 2037
Size: 722 1315
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 727 818 952 1073 1652 2037
Size: 167 560 91 134 121 579 385
Fragments arranged by size:
579 560 385 167 134 121 91
AhdI GACnn_n'nnGTC
Cuts at: 0 51 1254 1476 2037
Size: 51 1203 222 561
Fragments arranged by size:
1203 561 222 51
ApaI G_GGCC'C
Cuts at: 0 9 1000 1127 1680 2026 2037
Size: 9 991 127 553 346 11
Fragments arranged by size:
991 553 346 127 11 9
AvaI C'yCGr_G
Cuts at: 0 383 2037
Size: 383 1654
BamHI G'GATC_C
Cuts at: 0 534 2037
Size: 534 1503
BanI G'GyrC_C
Cuts at: 0 1921 2037
Size: 1921 116
BanII G_rGCy'C
Cuts at: 0 9 319 1000 1127 1680 2026 2037
Size: 9 310 681 127 553 346 11
Fragments arranged by size:
681 553 346 310 127 11 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 250 806 2037
Size: 250 556 1231
Fragments arranged by size:
1231 556 250
BbvCI CC'TCA_GC
Cuts at: 0 302 2037
Size: 302 1735
BglI GCCn_nnn'nGGC
Cuts at: 0 1675 1930 2037
Size: 1675 255 107
Fragments arranged by size:
1675 255 107
BglII A'GATC_T
Cuts at: 0 749 2037
Size: 749 1288
Bme1580I G_kGCm'C
Cuts at: 0 9 963 1000 1127 1680 2026 2037
Size: 9 954 37 127 553 346 11
Fragments arranged by size:
954 553 346 127 37 11 9
BmgBI CAC'GTC
Cuts at: 0 1820 2037
Size: 1820 217
BmrI ACTGGGnnnn_n'
Cuts at: 0 1542 2037
Size: 1542 495
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1666 1668 2037
Size: 1666 2 369
Fragments arranged by size:
1666 369 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 451 1129 1412 1481 1700 1766 2011
Size: 341 110 678 283 69 219 66 245
Cuts at: 2011 2037
Size: 26
Fragments arranged by size:
678 341 283 245 219 110 69 66
26
Bpu10I CC'TnA_GC
Cuts at: 0 302 2037
Size: 302 1735
BsaI GGTCTCn'nnnn_
Cuts at: 0 1380 2037
Size: 1380 657
BsaAI yAC'GTr
Cuts at: 0 1161 2037
Size: 1161 876
BsaHI Gr'CG_yC
Cuts at: 0 1492 2037
Size: 1492 545
BsaWI w'CCGG_w
Cuts at: 0 85 2037
Size: 85 1952
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1579 1609 2037
Size: 1579 30 428
Fragments arranged by size:
1579 428 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 134 509 571 1587 1651 2037
Size: 52 82 375 62 1016 64 386
Fragments arranged by size:
1016 386 375 82 64 62 52
BseYI C'CCAG_C
Cuts at: 0 654 1140 1661 1740 1961 2037
Size: 654 486 521 79 221 76
Fragments arranged by size:
654 521 486 221 79 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 923 2037
Size: 502 421 1114
Fragments arranged by size:
1114 502 421
BsiHKAI G_wGCw'C
Cuts at: 0 1903 2037
Size: 1903 134
BsmI GAATG_Cn'
Cuts at: 0 1193 1350 1962 2037
Size: 1193 157 612 75
Fragments arranged by size:
1193 612 157 75
BsmBI CGTCTCn'nnnn_
Cuts at: 0 617 1599 1996 2037
Size: 617 982 397 41
Fragments arranged by size:
982 617 397 41
Bsp1286I G_dGCh'C
Cuts at: 0 9 319 963 1000 1127 1680 1903 2026
Size: 9 310 644 37 127 553 223 123
Cuts at: 2026 2037
Size: 11
Fragments arranged by size:
644 553 310 223 127 123 37 11
9
BspEI T'CCGG_A
Cuts at: 0 85 2037
Size: 85 1952
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1226 2037
Size: 1226 811
BsrDI GCAATG_nn'
Cuts at: 0 639 2037
Size: 639 1398
BsrFI r'CCGG_y
Cuts at: 0 17 1967 2037
Size: 17 1950 70
Fragments arranged by size:
1950 70 17
BstEII G'GTnAC_C
Cuts at: 0 151 2037
Size: 151 1886
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1989 2037
Size: 1989 48
BstYI r'GATC_y
Cuts at: 0 534 749 971 2037
Size: 534 215 222 1066
Fragments arranged by size:
1066 534 222 215
BtgI C'CryG_G
Cuts at: 0 413 530 608 1562 2037
Size: 413 117 78 954 475
Fragments arranged by size:
954 475 413 117 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1602 1851 2037
Size: 1602 249 186
Fragments arranged by size:
1602 249 186
BtsI GCAGTG_nn'
Cuts at: 0 961 1212 2037
Size: 961 251 825
Fragments arranged by size:
961 825 251
DraIII CAC_nnn'GTG
Cuts at: 0 531 2037
Size: 531 1506
DrdI GACnn_nn'nnGTC
Cuts at: 0 1497 1945 2037
Size: 1497 448 92
Fragments arranged by size:
1497 448 92
EaeI y'GGCC_r
Cuts at: 0 636 1173 1212 2037
Size: 636 537 39 825
Fragments arranged by size:
825 636 537 39
EarI CTCTTCn'nnn_
Cuts at: 0 307 320 413 548 1049 1109 1326 1331
Size: 307 13 93 135 501 60 217 5
Cuts at: 1331 1628 1659 1778 2037
Size: 297 31 119 259
Fragments arranged by size:
501 307 297 259 217 135 119 93
60 31 13 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 341 451 727 818 952 1073 1129
Size: 167 174 110 276 91 134 121 56
Cuts at: 1129 1412 1481 1652 1700 1766 2011 2037
Size: 283 69 171 48 66 245 26
Fragments arranged by size:
283 276 245 174 171 167 134 121
110 91 69 66 56 48 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 742 2037
Size: 742 1295
EcoO109I rG'GnC_Cy
Cuts at: 0 5 6 997 1113 1124 1525 1676 1677
Size: 5 1 991 116 11 401 151 1
Cuts at: 1677 2009 2022 2023 2037
Size: 332 13 1 14
Fragments arranged by size:
991 401 332 151 116 14 13 11
5 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 577 2037
Size: 577 1460
FspI TGC'GCA
Cuts at: 0 1824 2037
Size: 1824 213
FspAI rTGC'GCAy
Cuts at: 0 1824 2037
Size: 1824 213
HaeII r_GCGC'y
Cuts at: 0 1968 1974 2037
Size: 1968 6 63
Fragments arranged by size:
1968 63 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 1048 1080 1227 1240 1259 1268
Size: 37 32 979 32 147 13 19 9
Cuts at: 1268 1272 1300 1462 1494 1579 1611 1777 1809
Size: 4 28 162 32 85 32 166 32
Cuts at: 1809 2037
Size: 228
Fragments arranged by size:
979 228 166 162 147 85 37 32
32 32 32 32 28 19 13 9
4
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1346 1933 2037
Size: 452 894 587 104
Fragments arranged by size:
894 587 452 104
MscI TGG'CCA
Cuts at: 0 638 1175 1214 2037
Size: 638 537 39 823
Fragments arranged by size:
823 638 537 39
MslI CAynn'nnrTG
Cuts at: 0 353 644 677 1561 2037
Size: 353 291 33 884 476
Fragments arranged by size:
884 476 353 291 33
MspA1I CmG'CkG
Cuts at: 0 1708 1744 2037
Size: 1708 36 293
Fragments arranged by size:
1708 293 36
NaeI GCC'GGC
Cuts at: 0 19 1969 2037
Size: 19 1950 68
Fragments arranged by size:
1950 68 19
NcoI C'CATG_G
Cuts at: 0 1562 2037
Size: 1562 475
NdeI CA'TA_TG
Cuts at: 0 1200 2037
Size: 1200 837
NgoMIV G'CCGG_C
Cuts at: 0 17 1967 2037
Size: 17 1950 70
Fragments arranged by size:
1950 70 17
NspI r_CATG'y
Cuts at: 0 607 2037
Size: 607 1430
PflMI CCAn_nnn'nTGG
Cuts at: 0 531 2037
Size: 531 1506
PfoI T'CCnGG_A
Cuts at: 0 851 2037
Size: 851 1186
PmlI CAC'GTG
Cuts at: 0 1161 2037
Size: 1161 876
PspOMI G'GGCC_C
Cuts at: 0 5 996 1123 1676 2022 2037
Size: 5 991 127 553 346 15
Fragments arranged by size:
991 553 346 127 15 5
PstI C_TGCA'G
Cuts at: 0 1360 2037
Size: 1360 677
PvuII CAG'CTG
Cuts at: 0 1744 2037
Size: 1744 293
SapI GCTCTTCn'nnn_
Cuts at: 0 307 1049 2037
Size: 307 742 988
Fragments arranged by size:
988 742 307
SexAI A'CCwGG_T
Cuts at: 0 1917 2037
Size: 1917 120
SfcI C'TryA_G
Cuts at: 0 1356 2037
Size: 1356 681
SgrAI Cr'CCGG_yG
Cuts at: 0 1967 2037
Size: 1967 70
SspI AAT'ATT
Cuts at: 0 1077 2037
Size: 1077 960
StyI C'CwwG_G
Cuts at: 0 1562 2037
Size: 1562 475
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 593 1812 2037
Size: 593 1219 225
Fragments arranged by size:
1219 593 225
TatI w'GTAC_w
Cuts at: 0 485 648 770 2037
Size: 485 163 122 1267
Fragments arranged by size:
1267 485 163 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 519 551 2037
Size: 519 32 1486
Fragments arranged by size:
1486 519 32
Tth111I GACn'n_nGTC
Cuts at: 0 727 1591 2037
Size: 727 864 446
Fragments arranged by size:
864 727 446
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1539 2037
Size: 1539 498
Enzymes that do cut:
AarI AccI AcuI AhdI ApaI AvaI BamHI BanI
BanII BbsI BbvCI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrDI BsrFI BstEII BstXI BstYI BtgI BtgZI
BtsI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I
FalI FspI FspAI HaeII Hin4I MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NspI PflMI PfoI
PmlI PspOMI PstI PvuII SapI SexAI SfcI SgrAI
SspI StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BcgI BciVI BclI BfrBI BlpI
BmtI BpuEI BsaBI BsiEI BsiWI BspHI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI
DraI EagI EciI EcoICRI EcoRI EcoRV FseI HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NheI
NotI NruI NsiI PacI PasI PciI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI
XbaI XhoI XmaI XmnI ZraI