Restriction map for
pF1KA1927
(Linear) (Six-base) MAPSORT of: KIAA1927.seq Check: 6072 from: 1 to: 3777
KIAA1927.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 1969 2524 3777
Size: 1969 555 1253
Fragments arranged by size:
1969 1253 555
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 560 1555 1556 1708 1886 2351 2702
Size: 380 180 995 1 152 178 465 351
Cuts at: 2702 3028 3262 3563 3596 3777
Size: 326 234 301 33 181
Fragments arranged by size:
995 465 380 351 326 301 234 181
180 178 152 33 1
AfeI AGC'GCT
Cuts at: 0 3165 3777
Size: 3165 612
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3116 3777
Size: 3116 661
AlwNI CAG_nnn'CTG
Cuts at: 0 98 1105 3051 3214 3310 3493 3619 3777
Size: 98 1007 1946 163 96 183 126 158
Fragments arranged by size:
1946 1007 183 163 158 126 98 96
ApaLI G'TGCA_C
Cuts at: 0 1343 3777
Size: 1343 2434
ApoI r'AATT_y
Cuts at: 0 462 545 596 906 1133 1957 2904 3024
Size: 462 83 51 310 227 824 947 120
Cuts at: 3024 3284 3777
Size: 260 493
Fragments arranged by size:
947 824 493 462 310 260 227 120
83 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3368 3401 3777
Size: 3368 33 376
Fragments arranged by size:
3368 376 33
BanI G'GyrC_C
Cuts at: 0 2448 3777
Size: 2448 1329
BanII G_rGCy'C
Cuts at: 0 2032 2094 2239 2882 3552 3777
Size: 2032 62 145 643 670 225
Fragments arranged by size:
2032 670 643 225 145 62
BbeI G_GCGC'C
Cuts at: 0 2452 3777
Size: 2452 1325
BbsI GAAGACnn'nnnn_
Cuts at: 0 1658 3777
Size: 1658 2119
BbvCI CC'TCA_GC
Cuts at: 0 3620 3777
Size: 3620 157
BclI T'GATC_A
Cuts at: 0 3003 3777
Size: 3003 774
BfrBI ATG'CAT
Cuts at: 0 3630 3777
Size: 3630 147
BglI GCCn_nnn'nGGC
Cuts at: 0 3080 3777
Size: 3080 697
Bme1580I G_kGCm'C
Cuts at: 0 221 1347 3777
Size: 221 1126 2430
Fragments arranged by size:
2430 1126 221
BmgBI CAC'GTC
Cuts at: 0 571 3777
Size: 571 3206
BmrI ACTGGGnnnn_n'
Cuts at: 0 1225 2054 2233 3777
Size: 1225 829 179 1544
Fragments arranged by size:
1544 1225 829 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 2261 2866 2995 3085 3106 3314 3401
Size: 64 2197 605 129 90 21 208 87
Cuts at: 3401 3491 3777
Size: 90 286
Fragments arranged by size:
2197 605 286 208 129 90 90 87
64 21
Bpu10I CC'TnA_GC
Cuts at: 0 3620 3777
Size: 3620 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 286 758 3777
Size: 229 57 472 3019
Fragments arranged by size:
3019 472 229 57
BsaI GGTCTCn'nnnn_
Cuts at: 0 2213 2281 3777
Size: 2213 68 1496
Fragments arranged by size:
2213 1496 68
BsaAI yAC'GTr
Cuts at: 0 1498 3777
Size: 1498 2279
BsaHI Gr'CG_yC
Cuts at: 0 787 2449 3777
Size: 787 1662 1328
Fragments arranged by size:
1662 1328 787
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 2034 2064 2977 3007 3777
Size: 109 30 1895 30 913 30 770
Fragments arranged by size:
1895 913 770 109 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 49 166 169 178 181 1427 1717 1720
Size: 49 117 3 9 3 1246 290 3
Cuts at: 1720 2350 2998 3001 3082 3103 3120 3777
Size: 630 648 3 81 21 17 657
Fragments arranged by size:
1246 657 648 630 290 117 81 49
21 17 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 3078 3777
Size: 3078 699
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3517 3777
Size: 3517 260
BsiHKAI G_wGCw'C
Cuts at: 0 666 1347 2032 2094 2582 2599 2704 2882
Size: 666 681 685 62 488 17 105 178
Cuts at: 2882 3777
Size: 895
Fragments arranged by size:
895 685 681 666 488 178 105 62
17
BsmI GAATG_Cn'
Cuts at: 0 1122 1512 2470 3777
Size: 1122 390 958 1307
Fragments arranged by size:
1307 1122 958 390
BsmBI CGTCTCn'nnnn_
Cuts at: 0 106 3777
Size: 106 3671
Bsp1286I G_dGCh'C
Cuts at: 0 221 666 1347 2032 2094 2239 2582 2599
Size: 221 445 681 685 62 145 343 17
Cuts at: 2599 2704 2882 3552 3777
Size: 105 178 670 225
Fragments arranged by size:
685 681 670 445 343 225 221 178
145 105 62 17
BspHI T'CATG_A
Cuts at: 0 1203 3777
Size: 1203 2574
BsrBI CCG'CTC
Cuts at: 0 3128 3777
Size: 3128 649
BsrDI GCAATG_nn'
Cuts at: 0 2948 3777
Size: 2948 829
BsrFI r'CCGG_y
Cuts at: 0 5 192 3777
Size: 5 187 3585
Fragments arranged by size:
3585 187 5
BssHII G'CGCG_C
Cuts at: 0 182 3777
Size: 182 3595
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1753 3777
Size: 1753 2024
BstBI TT'CG_AA
Cuts at: 0 1400 3777
Size: 1400 2377
BstYI r'GATC_y
Cuts at: 0 120 472 534 3777
Size: 120 352 62 3243
Fragments arranged by size:
3243 352 120 62
BstZ17I GTA'TAC
Cuts at: 0 1970 3777
Size: 1970 1807
BtgI C'CryG_G
Cuts at: 0 2060 2452 2960 3777
Size: 2060 392 508 817
Fragments arranged by size:
2060 817 508 392
BtsI GCAGTG_nn'
Cuts at: 0 869 1302 1530 1953 2025 2767 3777
Size: 869 433 228 423 72 742 1010
Fragments arranged by size:
1010 869 742 433 423 228 72
DraIII CAC_nnn'GTG
Cuts at: 0 1648 3777
Size: 1648 2129
EaeI y'GGCC_r
Cuts at: 0 416 746 3777
Size: 416 330 3031
Fragments arranged by size:
3031 416 330
EarI CTCTTCn'nnn_
Cuts at: 0 201 1403 1727 1868 2061 2327 2736 3777
Size: 201 1202 324 141 193 266 409 1041
Fragments arranged by size:
1202 1041 409 324 266 201 193 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 46 2910 3777
Size: 40 6 2864 867
Fragments arranged by size:
2864 867 40 6
EcoICRI GAG'CTC
Cuts at: 0 2030 2092 2880 3777
Size: 2030 62 788 897
Fragments arranged by size:
2030 897 788 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 380 560 1555 1556 1708 1886 2261
Size: 64 316 180 995 1 152 178 375
Cuts at: 2261 2351 2702 2866 2995 3028 3085 3106 3262
Size: 90 351 164 129 33 57 21 156
Cuts at: 3262 3314 3401 3491 3563 3596 3777
Size: 52 87 90 72 33 181
Fragments arranged by size:
995 375 351 316 181 180 178 164
156 152 129 90 90 87 72 64
57 52 33 33 21 1
EcoO109I rG'GnC_Cy
Cuts at: 0 171 2567 2803 3048 3565 3777
Size: 171 2396 236 245 517 212
Fragments arranged by size:
2396 517 245 236 212 171
EcoRI G'AATT_C
Cuts at: 0 462 3777
Size: 462 3315
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 565 1413 3777
Size: 565 848 2364
Fragments arranged by size:
2364 848 565
FspI TGC'GCA
Cuts at: 0 3109 3777
Size: 3109 668
FspAI rTGC'GCAy
Cuts at: 0 3109 3777
Size: 3109 668
HaeII r_GCGC'y
Cuts at: 0 2452 3075 3167 3777
Size: 2452 623 92 610
Fragments arranged by size:
2452 623 610 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 109 137 141 331 363 1009 1041
Size: 105 4 28 4 190 32 646 32
Cuts at: 1041 1540 1572 1693 1725 2014 2046 2362 2394
Size: 499 32 121 32 289 32 316 32
Cuts at: 2394 3160 3192 3777
Size: 766 32 585
Fragments arranged by size:
766 646 585 499 316 289 190 121
105 32 32 32 32 32 32 32
28 4 4
KasI G'GCGC_C
Cuts at: 0 2448 3777
Size: 2448 1329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 497 3777
Size: 89 408 3280
Fragments arranged by size:
3280 408 89
MscI TGG'CCA
Cuts at: 0 418 748 3777
Size: 418 330 3029
Fragments arranged by size:
3029 418 330
MslI CAynn'nnrTG
Cuts at: 0 374 380 2675 2728 3145 3635 3777
Size: 374 6 2295 53 417 490 142
Fragments arranged by size:
2295 490 417 374 142 53 6
MspA1I CmG'CkG
Cuts at: 0 189 199 961 1105 2454 3063 3541 3616
Size: 189 10 762 144 1349 609 478 75
Cuts at: 3616 3777
Size: 161
Fragments arranged by size:
1349 762 609 478 189 161 144 75
10
NaeI GCC'GGC
Cuts at: 0 194 3777
Size: 194 3583
NarI GG'CG_CC
Cuts at: 0 2449 3777
Size: 2449 1328
NcoI C'CATG_G
Cuts at: 0 2060 2960 3777
Size: 2060 900 817
Fragments arranged by size:
2060 900 817
NdeI CA'TA_TG
Cuts at: 0 2975 3777
Size: 2975 802
NgoMIV G'CCGG_C
Cuts at: 0 192 3777
Size: 192 3585
NruI TCG'CGA
Cuts at: 0 235 3777
Size: 235 3542
NsiI A_TGCA'T
Cuts at: 0 3632 3777
Size: 3632 145
NspI r_CATG'y
Cuts at: 0 2680 3114 3777
Size: 2680 434 663
Fragments arranged by size:
2680 663 434
PfoI T'CCnGG_A
Cuts at: 0 781 2277 3291 3777
Size: 781 1496 1014 486
Fragments arranged by size:
1496 1014 781 486
PpuMI rG'GwC_Cy
Cuts at: 0 171 2803 3048 3565 3777
Size: 171 2632 245 517 212
Fragments arranged by size:
2632 517 245 212 171
PsiI TTA'TAA
Cuts at: 0 554 3777
Size: 554 3223
PstI C_TGCA'G
Cuts at: 0 797 2774 3062 3777
Size: 797 1977 288 715
Fragments arranged by size:
1977 797 715 288
PvuII CAG'CTG
Cuts at: 0 189 961 1105 3063 3541 3616 3777
Size: 189 772 144 1958 478 75 161
Fragments arranged by size:
1958 772 478 189 161 144 75
SacI G_AGCT'C
Cuts at: 0 2032 2094 2882 3777
Size: 2032 62 788 895
Fragments arranged by size:
2032 895 788 62
SacII CC_GC'GG
Cuts at: 0 2455 3777
Size: 2455 1322
ScaI AGT'ACT
Cuts at: 0 2832 3777
Size: 2832 945
SfcI C'TryA_G
Cuts at: 0 793 1070 1544 1833 2770 3058 3777
Size: 793 277 474 289 937 288 719
Fragments arranged by size:
937 793 719 474 289 288 277
SfoI GGC'GCC
Cuts at: 0 2450 3777
Size: 2450 1327
SmlI C'TyrA_G
Cuts at: 0 244 301 773 3777
Size: 244 57 472 3004
Fragments arranged by size:
3004 472 244 57
SnaBI TAC'GTA
Cuts at: 0 1498 3777
Size: 1498 2279
SphI G_CATG'C
Cuts at: 0 3114 3777
Size: 3114 663
StuI AGG'CCT
Cuts at: 0 76 3777
Size: 76 3701
StyI C'CwwG_G
Cuts at: 0 1164 2060 2960 3777
Size: 1164 896 900 817
Fragments arranged by size:
1164 900 896 817
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1207 3777
Size: 1207 2570
TatI w'GTAC_w
Cuts at: 0 295 2321 2830 3777
Size: 295 2026 509 947
Fragments arranged by size:
2026 947 509 295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 471 503 1821 1853 3777
Size: 31 32 408 32 1318 32 1924
Fragments arranged by size:
1924 1318 408 32 32 32 31
XbaI T'CTAG_A
Cuts at: 0 3481 3777
Size: 3481 296
XmnI GAAnn'nnTTC
Cuts at: 0 545 1412 3442 3777
Size: 545 867 2030 335
Fragments arranged by size:
2030 867 545 335
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI ApaLI ApoI BaeI
BanI BanII BbeI BbsI BbvCI BclI BfrBI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BsrBI BsrDI BsrFI BssHII BstAPI BstBI
BstYI BstZ17I BtgI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NruI NsiI NspI PfoI PpuMI
PsiI PstI PvuII SacI SacII ScaI SfcI SfoI
SmlI SnaBI SphI StuI StyI TaqII TatI TstI
XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BamHI BcgI BciVI BglII BlpI BmtI BplI BsaBI
BsaWI BsiEI BsiWI BspEI BspMI BsrGI BssSI BstEII
BstXI Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EcoNI EcoRV FseI HincII HindIII HpaI KpnI MfeI
MluI NheI NotI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SpeI SrfI SspI SwaI Tth111I XcmI XhoI
XmaI ZraI