Restriction map for
pF1KSDA1934
(Linear) (Six-base) MAPSORT of: KIAA1934.seq Check: 6648 from: 1 to: 1344
KIAA1934.orf
With 182 enzymes: SgfI *
February 28, 2007 11:29 ..
AccI GT'mk_AC
Cuts at: 0 312 1059 1344
Size: 312 747 285
Fragments arranged by size:
747 312 285
AlwNI CAG_nnn'CTG
Cuts at: 0 135 1309 1344
Size: 135 1174 35
Fragments arranged by size:
1174 135 35
ApaLI G'TGCA_C
Cuts at: 0 820 1344
Size: 820 524
ApoI r'AATT_y
Cuts at: 0 101 1344
Size: 101 1243
AvaI C'yCGr_G
Cuts at: 0 881 1344
Size: 881 463
AvrII C'CTAG_G
Cuts at: 0 153 1344
Size: 153 1191
BanII G_rGCy'C
Cuts at: 0 369 803 832 923 1344
Size: 369 434 29 91 421
Fragments arranged by size:
434 421 369 91 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 182 186 533 1217 1344
Size: 182 4 347 684 127
Fragments arranged by size:
684 347 182 127 4
BciVI GTATCCnnnnn_n'
Cuts at: 0 1241 1344
Size: 1241 103
BglI GCCn_nnn'nGGC
Cuts at: 0 564 1344
Size: 564 780
BglII A'GATC_T
Cuts at: 0 698 1344
Size: 698 646
Bme1580I G_kGCm'C
Cuts at: 0 824 1344
Size: 824 520
BmrI ACTGGGnnnn_n'
Cuts at: 0 230 1344
Size: 230 1114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 792 1344
Size: 792 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 628 937 976 1238 1344
Size: 622 6 309 39 262 106
Fragments arranged by size:
622 309 262 106 39 6
BsaI GGTCTCn'nnnn_
Cuts at: 0 511 1344
Size: 511 833
BsaAI yAC'GTr
Cuts at: 0 613 1229 1344
Size: 613 616 115
Fragments arranged by size:
616 613 115
BsaHI Gr'CG_yC
Cuts at: 0 686 1344
Size: 686 658
BsaWI w'CCGG_w
Cuts at: 0 1054 1344
Size: 1054 290
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 643 673 758 788 1344
Size: 643 30 85 30 556
Fragments arranged by size:
643 556 85 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1300 1344
Size: 1300 44
BseYI C'CCAG_C
Cuts at: 0 1309 1344
Size: 1309 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1073 1126 1344
Size: 1073 53 218
Fragments arranged by size:
1073 218 53
BsiHKAI G_wGCw'C
Cuts at: 0 644 824 923 1344
Size: 644 180 99 421
Fragments arranged by size:
644 421 180 99
Bsp1286I G_dGCh'C
Cuts at: 0 369 644 803 824 832 923 1344
Size: 369 275 159 21 8 91 421
Fragments arranged by size:
421 369 275 159 91 21 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 531 1344
Size: 531 813
BstXI CCAn_nnnn'nTGG
Cuts at: 0 369 669 954 1344
Size: 369 300 285 390
Fragments arranged by size:
390 369 300 285
BstYI r'GATC_y
Cuts at: 0 427 698 1344
Size: 427 271 646
Fragments arranged by size:
646 427 271
Bsu36I CC'TnA_GG
Cuts at: 0 904 1094 1344
Size: 904 190 250
Fragments arranged by size:
904 250 190
BtgI C'CryG_G
Cuts at: 0 80 1215 1344
Size: 80 1135 129
Fragments arranged by size:
1135 129 80
BtsI GCAGTG_nn'
Cuts at: 0 10 682 1013 1197 1344
Size: 10 672 331 184 147
Fragments arranged by size:
672 331 184 147 10
EaeI y'GGCC_r
Cuts at: 0 359 1218 1344
Size: 359 859 126
Fragments arranged by size:
859 359 126
EarI CTCTTCn'nnn_
Cuts at: 0 509 580 980 1344
Size: 509 71 400 364
Fragments arranged by size:
509 400 364 71
EcoICRI GAG'CTC
Cuts at: 0 921 1344
Size: 921 423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 628 937 976 1238 1344
Size: 622 6 309 39 262 106
Fragments arranged by size:
622 309 262 106 39 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 506 1344
Size: 506 838
EcoO109I rG'GnC_Cy
Cuts at: 0 57 150 1049 1344
Size: 57 93 899 295
Fragments arranged by size:
899 295 93 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 141 210 717 849 1344
Size: 141 69 507 132 495
Fragments arranged by size:
507 495 141 132 69
FspI TGC'GCA
Cuts at: 0 141 844 1344
Size: 141 703 500
Fragments arranged by size:
703 500 141
FspAI rTGC'GCAy
Cuts at: 0 141 1344
Size: 141 1203
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 758 790 1015 1047 1344
Size: 758 32 225 32 297
Fragments arranged by size:
758 297 225 32 32
MscI TGG'CCA
Cuts at: 0 361 1220 1344
Size: 361 859 124
Fragments arranged by size:
859 361 124
MslI CAynn'nnrTG
Cuts at: 0 79 637 667 853 1344
Size: 79 558 30 186 491
Fragments arranged by size:
558 491 186 79 30
NcoI C'CATG_G
Cuts at: 0 80 1215 1344
Size: 80 1135 129
Fragments arranged by size:
1135 129 80
NdeI CA'TA_TG
Cuts at: 0 562 1344
Size: 562 782
PasI CC'CwG_GG
Cuts at: 0 369 1334 1344
Size: 369 965 10
Fragments arranged by size:
965 369 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 1334 1344
Size: 1334 10
PpuMI rG'GwC_Cy
Cuts at: 0 150 1344
Size: 150 1194
PstI C_TGCA'G
Cuts at: 0 749 812 1281 1344
Size: 749 63 469 63
Fragments arranged by size:
749 469 63 63
SacI G_AGCT'C
Cuts at: 0 923 1344
Size: 923 421
SfcI C'TryA_G
Cuts at: 0 745 808 1277 1344
Size: 745 63 469 67
Fragments arranged by size:
745 469 67 63
StyI C'CwwG_G
Cuts at: 0 80 153 185 1215 1235 1344
Size: 80 73 32 1030 20 109
Fragments arranged by size:
1030 109 80 73 32 20
Tth111I GACn'n_nGTC
Cuts at: 0 214 1344
Size: 214 1130
XmnI GAAnn'nnTTC
Cuts at: 0 322 495 1344
Size: 322 173 849
Fragments arranged by size:
849 322 173
Enzymes that do cut:
AccI AlwNI ApaLI ApoI AvaI AvrII BanII BbsI
BciVI BglI BglII Bme1580I BmrI BplI BpmI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BstXI BstYI Bsu36I BtgI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI
Hin4I MscI MslI NcoI NdeI PasI PflMI PpuMI
PstI SacI SfcI StyI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI BaeI BamHI BanI BbeI BbvCI BcgI BclI
BfrBI BlpI BmgBI BmtI Bpu10I BpuEI BsaBI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI
EcoRV FseI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI XbaI XcmI XhoI XmaI ZraI