Restriction map for
pF1KA1936
(Linear) (Six-base) MAPSORT of: KIAA1936.seq Check: 838 from: 1 to: 2412
KIAA1936.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1560 2412
Size: 1560 852
AatII G_ACGT'C
Cuts at: 0 64 2412
Size: 64 2348
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1480 2412
Size: 1480 932
AfeI AGC'GCT
Cuts at: 0 659 2412
Size: 659 1753
AflIII A'CryG_T
Cuts at: 0 311 370 1484 2412
Size: 311 59 1114 928
Fragments arranged by size:
1114 928 311 59
AhdI GACnn_n'nnGTC
Cuts at: 0 1001 2412
Size: 1001 1411
AleI CACnn'nnGTG
Cuts at: 0 1639 2412
Size: 1639 773
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 803 1588 1872 2412
Size: 803 785 284 540
Fragments arranged by size:
803 785 540 284
AlwNI CAG_nnn'CTG
Cuts at: 0 259 558 1764 2037 2412
Size: 259 299 1206 273 375
Fragments arranged by size:
1206 375 299 273 259
ApaI G_GGCC'C
Cuts at: 0 1838 2412
Size: 1838 574
ApoI r'AATT_y
Cuts at: 0 232 1318 2412
Size: 232 1086 1094
Fragments arranged by size:
1094 1086 232
AseI AT'TA_AT
Cuts at: 0 1241 2412
Size: 1241 1171
AvaI C'yCGr_G
Cuts at: 0 2117 2412
Size: 2117 295
AvrII C'CTAG_G
Cuts at: 0 1182 2412
Size: 1182 1230
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 391 424 2412
Size: 391 33 1988
Fragments arranged by size:
1988 391 33
BanI G'GyrC_C
Cuts at: 0 2045 2412
Size: 2045 367
BanII G_rGCy'C
Cuts at: 0 773 986 1838 2231 2354 2412
Size: 773 213 852 393 123 58
Fragments arranged by size:
852 773 393 213 123 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 1266 2412
Size: 1266 1146
BbvCI CC'TCA_GC
Cuts at: 0 661 1765 2300 2412
Size: 661 1104 535 112
Fragments arranged by size:
1104 661 535 112
BciVI GTATCCnnnnn_n'
Cuts at: 0 419 2412
Size: 419 1993
BglII A'GATC_T
Cuts at: 0 1912 2246 2412
Size: 1912 334 166
Fragments arranged by size:
1912 334 166
Bme1580I G_kGCm'C
Cuts at: 0 1838 2412
Size: 1838 574
BmgBI CAC'GTC
Cuts at: 0 371 1452 1893 2412
Size: 371 1081 441 519
Fragments arranged by size:
1081 519 441 371
BmrI ACTGGGnnnn_n'
Cuts at: 0 1039 2412
Size: 1039 1373
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1079 2412
Size: 1079 1333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2338 2412
Size: 2338 74
Bpu10I CC'TnA_GC
Cuts at: 0 661 1508 1765 2281 2300 2412
Size: 661 847 257 516 19 112
Fragments arranged by size:
847 661 516 257 112 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 667 2412
Size: 117 550 1745
Fragments arranged by size:
1745 550 117
BsaI GGTCTCn'nnnn_
Cuts at: 0 85 1210 1775 1956 2412
Size: 85 1125 565 181 456
Fragments arranged by size:
1125 565 456 181 85
BsaAI yAC'GTr
Cuts at: 0 442 2412
Size: 442 1970
BsaHI Gr'CG_yC
Cuts at: 0 61 1675 2057 2412
Size: 61 1614 382 355
Fragments arranged by size:
1614 382 355 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 248 278 1877 1907 2296 2326 2412
Size: 248 30 1599 30 389 30 86
Fragments arranged by size:
1599 389 248 86 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 108 191 682 1398 2184 2317 2412
Size: 108 83 491 716 786 133 95
Fragments arranged by size:
786 716 491 133 108 95 83
BseYI C'CCAG_C
Cuts at: 0 624 2412
Size: 624 1788
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1328 1371 1940 2302 2412
Size: 799 529 43 569 362 110
Fragments arranged by size:
799 569 529 362 110 43
BsiHKAI G_wGCw'C
Cuts at: 0 773 986 1316 1332 2084 2231 2287 2354
Size: 773 213 330 16 752 147 56 67
Cuts at: 2354 2412
Size: 58
Fragments arranged by size:
773 752 330 213 147 67 58 56
16
BsmI GAATG_Cn'
Cuts at: 0 1849 2412
Size: 1849 563
BsmBI CGTCTCn'nnnn_
Cuts at: 0 361 2412
Size: 361 2051
Bsp1286I G_dGCh'C
Cuts at: 0 773 986 1316 1332 1838 2084 2231 2287
Size: 773 213 330 16 506 246 147 56
Cuts at: 2287 2354 2412
Size: 67 58
Fragments arranged by size:
773 506 330 246 213 147 67 58
56 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1560 1968 2412
Size: 1560 408 444
Fragments arranged by size:
1560 444 408
BsrBI CCG'CTC
Cuts at: 0 335 2412
Size: 335 2077
BsrDI GCAATG_nn'
Cuts at: 0 1046 2412
Size: 1046 1366
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1865 2412
Size: 1865 547
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1717 2412
Size: 1717 695
BstYI r'GATC_y
Cuts at: 0 3 717 1912 2246 2412
Size: 3 714 1195 334 166
Fragments arranged by size:
1195 714 334 166 3
Bsu36I CC'TnA_GG
Cuts at: 0 137 1054 2133 2412
Size: 137 917 1079 279
Fragments arranged by size:
1079 917 279 137
BtgI C'CryG_G
Cuts at: 0 2087 2196 2329 2412
Size: 2087 109 133 83
Fragments arranged by size:
2087 133 109 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1486 1528 2412
Size: 1486 42 884
Fragments arranged by size:
1486 884 42
BtsI GCAGTG_nn'
Cuts at: 0 269 1433 1710 2412
Size: 269 1164 277 702
Fragments arranged by size:
1164 702 277 269
DraI TTT'AAA
Cuts at: 0 725 2412
Size: 725 1687
DraIII CAC_nnn'GTG
Cuts at: 0 1033 2088 2412
Size: 1033 1055 324
Fragments arranged by size:
1055 1033 324
DrdI GACnn_nn'nnGTC
Cuts at: 0 862 1948 2412
Size: 862 1086 464
Fragments arranged by size:
1086 862 464
EaeI y'GGCC_r
Cuts at: 0 635 911 2412
Size: 635 276 1501
Fragments arranged by size:
1501 635 276
EarI CTCTTCn'nnn_
Cuts at: 0 126 352 2236 2412
Size: 126 226 1884 176
Fragments arranged by size:
1884 226 176 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1917 2412
Size: 1917 495
EcoICRI GAG'CTC
Cuts at: 0 771 984 2229 2352 2412
Size: 771 213 1245 123 60
Fragments arranged by size:
1245 771 213 123 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1480 2338 2412
Size: 1480 858 74
Fragments arranged by size:
1480 858 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 725 778 1010 2412
Size: 725 53 232 1402
Fragments arranged by size:
1402 725 232 53
EcoO109I rG'GnC_Cy
Cuts at: 0 876 1537 1834 1965 2401 2412
Size: 876 661 297 131 436 11
Fragments arranged by size:
876 661 436 297 131 11
EcoRV GAT'ATC
Cuts at: 0 105 2412
Size: 105 2307
HaeII r_GCGC'y
Cuts at: 0 661 1876 2412
Size: 661 1215 536
Fragments arranged by size:
1215 661 536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 298 330 987 1019 2296 2328
Size: 10 32 256 32 657 32 1277 32
Cuts at: 2328 2412
Size: 84
Fragments arranged by size:
1277 657 256 84 32 32 32 32
10
HincII GTy'rAC
Cuts at: 0 1061 2412
Size: 1061 1351
HindIII A'AGCT_T
Cuts at: 0 618 1105 1996 2412
Size: 618 487 891 416
Fragments arranged by size:
891 618 487 416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2357 2412
Size: 2357 55
MscI TGG'CCA
Cuts at: 0 637 913 2412
Size: 637 276 1499
Fragments arranged by size:
1499 637 276
MslI CAynn'nnrTG
Cuts at: 0 1639 1715 2412
Size: 1639 76 697
Fragments arranged by size:
1639 697 76
MspA1I CmG'CkG
Cuts at: 0 1683 2034 2412
Size: 1683 351 378
Fragments arranged by size:
1683 378 351
NcoI C'CATG_G
Cuts at: 0 2329 2412
Size: 2329 83
NspI r_CATG'y
Cuts at: 0 315 1488 2412
Size: 315 1173 924
Fragments arranged by size:
1173 924 315
PasI CC'CwG_GG
Cuts at: 0 465 2412
Size: 465 1947
PciI A'CATG_T
Cuts at: 0 311 1484 2412
Size: 311 1173 928
Fragments arranged by size:
1173 928 311
PflMI CCAn_nnn'nTGG
Cuts at: 0 1228 2412
Size: 1228 1184
PfoI T'CCnGG_A
Cuts at: 0 1227 2345 2412
Size: 1227 1118 67
Fragments arranged by size:
1227 1118 67
PpuMI rG'GwC_Cy
Cuts at: 0 876 1537 1965 2412
Size: 876 661 428 447
Fragments arranged by size:
876 661 447 428
PsiI TTA'TAA
Cuts at: 0 1265 2412
Size: 1265 1147
PspOMI G'GGCC_C
Cuts at: 0 1834 2412
Size: 1834 578
PstI C_TGCA'G
Cuts at: 0 264 479 563 1833 2412
Size: 264 215 84 1270 579
Fragments arranged by size:
1270 579 264 215 84
SacI G_AGCT'C
Cuts at: 0 773 986 2231 2354 2412
Size: 773 213 1245 123 58
Fragments arranged by size:
1245 773 213 123 58
SapI GCTCTTCn'nnn_
Cuts at: 0 2236 2412
Size: 2236 176
SexAI A'CCwGG_T
Cuts at: 0 1946 2412
Size: 1946 466
SfcI C'TryA_G
Cuts at: 0 83 210 260 475 559 1623 1829 1969
Size: 83 127 50 215 84 1064 206 140
Cuts at: 1969 2164 2396 2412
Size: 195 232 16
Fragments arranged by size:
1064 232 215 206 195 140 127 84
83 50 16
SmaI CCC'GGG
Cuts at: 0 2119 2412
Size: 2119 293
SmlI C'TyrA_G
Cuts at: 0 96 646 2412
Size: 96 550 1766
Fragments arranged by size:
1766 550 96
SnaBI TAC'GTA
Cuts at: 0 442 2412
Size: 442 1970
SrfI GCCC'GGGC
Cuts at: 0 2119 2412
Size: 2119 293
StuI AGG'CCT
Cuts at: 0 297 976 2412
Size: 297 679 1436
Fragments arranged by size:
1436 679 297
StyI C'CwwG_G
Cuts at: 0 1182 2329 2412
Size: 1182 1147 83
Fragments arranged by size:
1182 1147 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1021 2173 2403 2412
Size: 1021 1152 230 9
Fragments arranged by size:
1152 1021 230 9
TatI w'GTAC_w
Cuts at: 0 269 2412
Size: 269 2143
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 248 280 1877 1909 2188 2220 2412
Size: 248 32 1597 32 279 32 192
Fragments arranged by size:
1597 279 248 192 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2214 2412
Size: 2214 198
XmaI C'CCGG_G
Cuts at: 0 2117 2412
Size: 2117 295
ZraI GAC'GTC
Cuts at: 0 62 2412
Size: 62 2350
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApoI AseI AvaI AvrII BaeI BanI
BanII BbsI BbvCI BciVI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NcoI NspI PasI PciI
PflMI PfoI PpuMI PsiI PspOMI PstI SacI SapI
SexAI SfcI SmaI SmlI SnaBI SrfI StuI StyI
TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AloI ApaLI AscI
AsiSI BamHI BbeI BcgI BclI BfrBI BglI BlpI
BmtI BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI
EagI EcoRI FalI FseI FspI FspAI HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PmeI PmlI PpiI PshAI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SpeI SphI
SspI SwaI Tth111I XbaI XhoI XmnI