Restriction map for
pF1KA1938
(Linear) (Six-base) MAPSORT of: KIAA1938.seq Check: 2284 from: 1 to: 4029
KIAA1938.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AccI GT'mk_AC
Cuts at: 0 779 1418 1577 4029
Size: 779 639 159 2452
Fragments arranged by size:
2452 779 639 159
Acc65I G'GTAC_C
Cuts at: 0 1085 4029
Size: 1085 2944
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 1714 2146 2290 3406 4029
Size: 45 1669 432 144 1116 623
Fragments arranged by size:
1669 1116 623 432 144 45
AflIII A'CryG_T
Cuts at: 0 3629 4029
Size: 3629 400
AgeI A'CCGG_T
Cuts at: 0 1421 4029
Size: 1421 2608
AhdI GACnn_n'nnGTC
Cuts at: 0 253 2307 4029
Size: 253 2054 1722
Fragments arranged by size:
2054 1722 253
AleI CACnn'nnGTG
Cuts at: 0 2900 4029
Size: 2900 1129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 256 3814 4029
Size: 256 3558 215
Fragments arranged by size:
3558 256 215
AlwNI CAG_nnn'CTG
Cuts at: 0 477 1896 2760 3145 3451 4029
Size: 477 1419 864 385 306 578
Fragments arranged by size:
1419 864 578 477 385 306
ApaI G_GGCC'C
Cuts at: 0 513 4029
Size: 513 3516
ApaLI G'TGCA_C
Cuts at: 0 625 1346 1590 4029
Size: 625 721 244 2439
Fragments arranged by size:
2439 721 625 244
ApoI r'AATT_y
Cuts at: 0 1534 4029
Size: 1534 2495
AvaI C'yCGr_G
Cuts at: 0 11 153 312 812 1152 1683 2321 4029
Size: 11 142 159 500 340 531 638 1708
Fragments arranged by size:
1708 638 531 500 340 159 142 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 828 861 4029
Size: 828 33 3168
Fragments arranged by size:
3168 828 33
BanI G'GyrC_C
Cuts at: 0 358 1064 1085 4029
Size: 358 706 21 2944
Fragments arranged by size:
2944 706 358 21
BanII G_rGCy'C
Cuts at: 0 513 593 851 1153 1310 2066 2912 4029
Size: 513 80 258 302 157 756 846 1117
Fragments arranged by size:
1117 846 756 513 302 258 157 80
BbeI G_GCGC'C
Cuts at: 0 1068 4029
Size: 1068 2961
BbsI GAAGACnn'nnnn_
Cuts at: 0 909 4029
Size: 909 3120
BbvCI CC'TCA_GC
Cuts at: 0 578 2228 2716 3325 4029
Size: 578 1650 488 609 704
Fragments arranged by size:
1650 704 609 578 488
BglI GCCn_nnn'nGGC
Cuts at: 0 378 1057 4029
Size: 378 679 2972
Fragments arranged by size:
2972 679 378
BglII A'GATC_T
Cuts at: 0 1997 4029
Size: 1997 2032
BlpI GC'TnA_GC
Cuts at: 0 2107 3842 4029
Size: 2107 1735 187
Fragments arranged by size:
2107 1735 187
Bme1580I G_kGCm'C
Cuts at: 0 363 513 629 966 1350 1594 3513 4029
Size: 363 150 116 337 384 244 1919 516
Fragments arranged by size:
1919 516 384 363 337 244 150 116
BmrI ACTGGGnnnn_n'
Cuts at: 0 1808 4029
Size: 1808 2221
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 26 558 2979 3624 4029
Size: 26 532 2421 645 405
Fragments arranged by size:
2421 645 532 405 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1978 3055 3167 3742 4029
Size: 1978 1077 112 575 287
Fragments arranged by size:
1978 1077 575 287 112
Bpu10I CC'TnA_GC
Cuts at: 0 397 578 1164 2228 2716 3325 3742 3881
Size: 397 181 586 1064 488 609 417 139
Cuts at: 3881 4029
Size: 148
Fragments arranged by size:
1064 609 586 488 417 397 181 148
139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1501 2598 3523 4029
Size: 1501 1097 925 506
Fragments arranged by size:
1501 1097 925 506
BsaI GGTCTCn'nnnn_
Cuts at: 0 14 3477 4029
Size: 14 3463 552
Fragments arranged by size:
3463 552 14
BsaHI Gr'CG_yC
Cuts at: 0 1065 2667 4029
Size: 1065 1602 1362
Fragments arranged by size:
1602 1362 1065
BsaWI w'CCGG_w
Cuts at: 0 212 452 1421 3638 4029
Size: 212 240 969 2217 391
Fragments arranged by size:
2217 969 391 240 212
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 309 339 4029
Size: 309 30 3690
Fragments arranged by size:
3690 309 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 231 330 1348 2140 3610 3613 3823 3826
Size: 231 99 1018 792 1470 3 210 3
Cuts at: 3826 4029
Size: 203
Fragments arranged by size:
1470 1018 792 231 210 203 99 3
3
BseYI C'CCAG_C
Cuts at: 0 1060 1797 2102 2130 2753 3960 4029
Size: 1060 737 305 28 623 1207 69
Fragments arranged by size:
1207 1060 737 623 305 69 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 1316 2445 2593 3610 4029
Size: 593 723 1129 148 1017 419
Fragments arranged by size:
1129 1017 723 593 419 148
BsiEI CG_ry'CG
Cuts at: 0 710 2594 4029
Size: 710 1884 1435
Fragments arranged by size:
1884 1435 710
BsiHKAI G_wGCw'C
Cuts at: 0 593 629 851 932 1350 1442 1548 1594
Size: 593 36 222 81 418 92 106 46
Cuts at: 1594 1616 2066 4029
Size: 22 450 1963
Fragments arranged by size:
1963 593 450 418 222 106 92 81
46 36 22
BsmI GAATG_Cn'
Cuts at: 0 3408 4029
Size: 3408 621
BsmBI CGTCTCn'nnnn_
Cuts at: 0 240 4029
Size: 240 3789
Bsp1286I G_dGCh'C
Cuts at: 0 363 513 593 629 851 932 966 1153
Size: 363 150 80 36 222 81 34 187
Cuts at: 1153 1310 1350 1442 1548 1594 1616 2066 2912
Size: 157 40 92 106 46 22 450 846
Cuts at: 2912 3513 4029
Size: 601 516
Fragments arranged by size:
846 601 516 450 363 222 187 157
150 106 92 81 80 46 40 36
34 22
BspHI T'CATG_A
Cuts at: 0 546 1943 4029
Size: 546 1397 2086
Fragments arranged by size:
2086 1397 546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 956 1634 2612 3089 4029
Size: 956 678 978 477 940
Fragments arranged by size:
978 956 940 678 477
BsrBI CCG'CTC
Cuts at: 0 896 2217 3158 3456 3522 3621 3660 4029
Size: 896 1321 941 298 66 99 39 369
Fragments arranged by size:
1321 941 896 369 298 99 66 39
BsrDI GCAATG_nn'
Cuts at: 0 1874 3199 4029
Size: 1874 1325 830
Fragments arranged by size:
1874 1325 830
BsrFI r'CCGG_y
Cuts at: 0 264 951 1421 3569 4029
Size: 264 687 470 2148 460
Fragments arranged by size:
2148 687 470 460 264
BssSI C'ACGA_G
Cuts at: 0 2463 4029
Size: 2463 1566
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2499 3090 4029
Size: 2499 591 939
Fragments arranged by size:
2499 939 591
BstBI TT'CG_AA
Cuts at: 0 2581 4029
Size: 2581 1448
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2683 2902 4029
Size: 2683 219 1127
Fragments arranged by size:
2683 1127 219
BstYI r'GATC_y
Cuts at: 0 473 1117 1997 4029
Size: 473 644 880 2032
Fragments arranged by size:
2032 880 644 473
Bsu36I CC'TnA_GG
Cuts at: 0 3138 4029
Size: 3138 891
BtgI C'CryG_G
Cuts at: 0 282 1238 2832 2850 2937 3310 3836 3940
Size: 282 956 1594 18 87 373 526 104
Cuts at: 3940 4029
Size: 89
Fragments arranged by size:
1594 956 526 373 282 104 89 87
18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 58 1734 4029
Size: 58 1676 2295
Fragments arranged by size:
2295 1676 58
BtsI GCAGTG_nn'
Cuts at: 0 576 1087 1116 1671 2071 2137 2580 4029
Size: 576 511 29 555 400 66 443 1449
Fragments arranged by size:
1449 576 555 511 443 400 66 29
ClaI AT'CG_AT
Cuts at: 0 3556 4029
Size: 3556 473
DraIII CAC_nnn'GTG
Cuts at: 0 1677 4029
Size: 1677 2352
DrdI GACnn_nn'nnGTC
Cuts at: 0 1502 4029
Size: 1502 2527
EaeI y'GGCC_r
Cuts at: 0 707 1049 1403 1898 2556 2591 2829 2847
Size: 707 342 354 495 658 35 238 18
Cuts at: 2847 3253 4029
Size: 406 776
Fragments arranged by size:
776 707 658 495 406 354 342 238
35 18
EagI C'GGCC_G
Cuts at: 0 707 2591 4029
Size: 707 1884 1438
Fragments arranged by size:
1884 1438 707
EarI CTCTTCn'nnn_
Cuts at: 0 316 1478 1783 2797 2970 3521 3644 3730
Size: 316 1162 305 1014 173 551 123 86
Cuts at: 3730 3859 4029
Size: 129 170
Fragments arranged by size:
1162 1014 551 316 305 173 170 129
123 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3383 4029
Size: 3383 646
EcoICRI GAG'CTC
Cuts at: 0 591 849 2064 4029
Size: 591 258 1215 1965
Fragments arranged by size:
1965 1215 591 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 1714 1978 2146 2290 3055 3167 3406
Size: 45 1669 264 168 144 765 112 239
Cuts at: 3406 3742 4029
Size: 336 287
Fragments arranged by size:
1669 765 336 287 264 239 168 144
112 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 643 1654 2674 3328 4029
Size: 643 1011 1020 654 701
Fragments arranged by size:
1020 1011 701 654 643
EcoO109I rG'GnC_Cy
Cuts at: 0 76 2283 2601 2818 2958 2973 3142 4029
Size: 76 2207 318 217 140 15 169 887
Fragments arranged by size:
2207 887 318 217 169 140 76 15
EcoRI G'AATT_C
Cuts at: 0 1534 4029
Size: 1534 2495
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1947 4029
Size: 1947 2082
FspI TGC'GCA
Cuts at: 0 1728 4029
Size: 1728 2301
HaeII r_GCGC'y
Cuts at: 0 1068 1772 4029
Size: 1068 704 2257
Fragments arranged by size:
2257 1068 704
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 5 37 239 271 465 497 717 749
Size: 5 32 202 32 194 32 220 32
Cuts at: 749 1523 1555 1989 2021 2293 2325 3693 3725
Size: 774 32 434 32 272 32 1368 32
Cuts at: 3725 4029
Size: 304
Fragments arranged by size:
1368 774 434 304 272 220 202 194
32 32 32 32 32 32 32 32
5
HincII GTy'rAC
Cuts at: 0 1665 2511 3227 4029
Size: 1665 846 716 802
Fragments arranged by size:
1665 846 802 716
HindIII A'AGCT_T
Cuts at: 0 525 4029
Size: 525 3504
KasI G'GCGC_C
Cuts at: 0 1064 4029
Size: 1064 2965
KpnI G_GTAC'C
Cuts at: 0 1089 4029
Size: 1089 2940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 3500 3659 3934 4029
Size: 109 3391 159 275 95
Fragments arranged by size:
3391 275 159 109 95
MscI TGG'CCA
Cuts at: 0 1051 1405 1900 2849 4029
Size: 1051 354 495 949 1180
Fragments arranged by size:
1180 1051 949 495 354
MslI CAynn'nnrTG
Cuts at: 0 545 1405 2900 4029
Size: 545 860 1495 1129
Fragments arranged by size:
1495 1129 860 545
MspA1I CmG'CkG
Cuts at: 0 165 577 741 3170 3263 3353 3362 3849
Size: 165 412 164 2429 93 90 9 487
Cuts at: 3849 4029
Size: 180
Fragments arranged by size:
2429 487 412 180 165 164 93 90
9
NaeI GCC'GGC
Cuts at: 0 953 3571 4029
Size: 953 2618 458
Fragments arranged by size:
2618 953 458
NarI GG'CG_CC
Cuts at: 0 1065 4029
Size: 1065 2964
NcoI C'CATG_G
Cuts at: 0 1238 2832 2850 2937 3836 4029
Size: 1238 1594 18 87 899 193
Fragments arranged by size:
1594 1238 899 193 87 18
NgoMIV G'CCGG_C
Cuts at: 0 951 3569 4029
Size: 951 2618 460
Fragments arranged by size:
2618 951 460
NspI r_CATG'y
Cuts at: 0 867 2080 2712 3093 3483 3633 4029
Size: 867 1213 632 381 390 150 396
Fragments arranged by size:
1213 867 632 396 390 381 150
PasI CC'CwG_GG
Cuts at: 0 331 2676 2914 4029
Size: 331 2345 238 1115
Fragments arranged by size:
2345 1115 331 238
PciI A'CATG_T
Cuts at: 0 3629 4029
Size: 3629 400
PflMI CCAn_nnn'nTGG
Cuts at: 0 1896 2395 2938 3295 4029
Size: 1896 499 543 357 734
Fragments arranged by size:
1896 734 543 499 357
PfoI T'CCnGG_A
Cuts at: 0 206 3071 4029
Size: 206 2865 958
Fragments arranged by size:
2865 958 206
PpuMI rG'GwC_Cy
Cuts at: 0 76 2283 2601 2818 2958 2973 4029
Size: 76 2207 318 217 140 15 1056
Fragments arranged by size:
2207 1056 318 217 140 76 15
PspOMI G'GGCC_C
Cuts at: 0 509 4029
Size: 509 3520
PspXI vC'TCGA_Gb
Cuts at: 0 312 2321 4029
Size: 312 2009 1708
Fragments arranged by size:
2009 1708 312
PstI C_TGCA'G
Cuts at: 0 740 1367 3086 3989 4029
Size: 740 627 1719 903 40
Fragments arranged by size:
1719 903 740 627 40
PvuII CAG'CTG
Cuts at: 0 165 577 4029
Size: 165 412 3452
Fragments arranged by size:
3452 412 165
SacI G_AGCT'C
Cuts at: 0 593 851 2066 4029
Size: 593 258 1215 1963
Fragments arranged by size:
1963 1215 593 258
SanDI GG'GwC_CC
Cuts at: 0 76 2973 4029
Size: 76 2897 1056
Fragments arranged by size:
2897 1056 76
ScaI AGT'ACT
Cuts at: 0 3547 4029
Size: 3547 482
SfcI C'TryA_G
Cuts at: 0 736 1363 2943 3015 3082 3985 4029
Size: 736 627 1580 72 67 903 44
Fragments arranged by size:
1580 903 736 627 72 67 44
SfoI GGC'GCC
Cuts at: 0 1066 4029
Size: 1066 2963
SmaI CCC'GGG
Cuts at: 0 814 4029
Size: 814 3215
SmlI C'TyrA_G
Cuts at: 0 11 312 1516 2321 2613 3538 4029
Size: 11 301 1204 805 292 925 491
Fragments arranged by size:
1204 925 805 491 301 292 11
SpeI A'CTAG_T
Cuts at: 0 1350 4029
Size: 1350 2679
SphI G_CATG'C
Cuts at: 0 2080 2712 4029
Size: 2080 632 1317
Fragments arranged by size:
2080 1317 632
StuI AGG'CCT
Cuts at: 0 2327 2623 4029
Size: 2327 296 1406
Fragments arranged by size:
2327 1406 296
StyI C'CwwG_G
Cuts at: 0 1238 1382 1880 2369 2639 2832 2850 2937
Size: 1238 144 498 489 270 193 18 87
Cuts at: 2937 3836 3902 4029
Size: 899 66 127
Fragments arranged by size:
1238 899 498 489 270 193 144 127
87 66 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 689 2716 4029
Size: 689 2027 1313
Fragments arranged by size:
2027 1313 689
TatI w'GTAC_w
Cuts at: 0 344 1369 3530 3545 4029
Size: 344 1025 2161 15 484
Fragments arranged by size:
2161 1025 484 344 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 309 341 1278 1310 3079 3102 3111 3134
Size: 309 32 937 32 1769 23 9 23
Cuts at: 3134 4029
Size: 895
Fragments arranged by size:
1769 937 895 309 32 32 23 23
9
Tth111I GACn'n_nGTC
Cuts at: 0 913 2743 4029
Size: 913 1830 1286
Fragments arranged by size:
1830 1286 913
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2899 3112 3642 4029
Size: 2899 213 530 387
Fragments arranged by size:
2899 530 387 213
XhoI C'TCGA_G
Cuts at: 0 11 312 2321 4029
Size: 11 301 2009 1708
Fragments arranged by size:
2009 1708 301 11
XmaI C'CCGG_G
Cuts at: 0 812 4029
Size: 812 3217
Enzymes that do cut:
AccI Acc65I AcuI AflIII AgeI AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BaeI BanI BanII
BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PpuMI
PspOMI PspXI PstI PvuII SacI SanDI ScaI SfcI
SfoI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AloI AscI AseI
AsiSI AvrII BamHI BcgI BciVI BclI BfrBI BmgBI
BmtI BsaAI BsaBI BsiWI BspEI BsrGI BssHII BstEII
BstZ17I CspCI DraI EcoRV FseI FspAI HpaI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI
SrfI SspI SwaI XbaI XmnI ZraI