Restriction map for
pF1KSDA1942
(Linear) (Six-base) MAPSORT of: KIAA1942.seq Check: 1409 from: 1 to: 1338
KIAA1942.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 543 1338
Size: 543 795
AatII G_ACGT'C
Cuts at: 0 932 1338
Size: 932 406
AccI GT'mk_AC
Cuts at: 0 95 1338
Size: 95 1243
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1270 1338
Size: 1270 68
AflIII A'CryG_T
Cuts at: 0 28 1338
Size: 28 1310
AgeI A'CCGG_T
Cuts at: 0 679 694 1338
Size: 679 15 644
Fragments arranged by size:
679 644 15
AleI CACnn'nnGTG
Cuts at: 0 446 1338
Size: 446 892
AlwNI CAG_nnn'CTG
Cuts at: 0 576 1218 1221 1338
Size: 576 642 3 117
Fragments arranged by size:
642 576 117 3
ApaI G_GGCC'C
Cuts at: 0 85 993 1338
Size: 85 908 345
Fragments arranged by size:
908 345 85
ApaLI G'TGCA_C
Cuts at: 0 1228 1338
Size: 1228 110
AvaI C'yCGr_G
Cuts at: 0 671 1113 1338
Size: 671 442 225
Fragments arranged by size:
671 442 225
BanI G'GyrC_C
Cuts at: 0 187 435 1082 1265 1338
Size: 187 248 647 183 73
Fragments arranged by size:
647 248 187 183 73
BanII G_rGCy'C
Cuts at: 0 85 569 962 993 1286 1338
Size: 85 484 393 31 293 52
Fragments arranged by size:
484 393 293 85 52 31
BbeI G_GCGC'C
Cuts at: 0 191 1338
Size: 191 1147
BbvCI CC'TCA_GC
Cuts at: 0 416 1338
Size: 416 922
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 634 668 1338
Size: 634 34 670
Fragments arranged by size:
670 634 34
BglII A'GATC_T
Cuts at: 0 998 1338
Size: 998 340
BlpI GC'TnA_GC
Cuts at: 0 840 1338
Size: 840 498
Bme1580I G_kGCm'C
Cuts at: 0 85 440 993 1232 1279 1338
Size: 85 355 553 239 47 59
Fragments arranged by size:
553 355 239 85 59 47
BmgBI CAC'GTC
Cuts at: 0 1179 1338
Size: 1179 159
BmrI ACTGGGnnnn_n'
Cuts at: 0 1027 1338
Size: 1027 311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 966 1338
Size: 442 524 372
Fragments arranged by size:
524 442 372
Bpu10I CC'TnA_GC
Cuts at: 0 416 1338
Size: 416 922
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1338
Size: 979 359
BsaI GGTCTCn'nnnn_
Cuts at: 0 1234 1338
Size: 1234 104
BsaAI yAC'GTr
Cuts at: 0 29 753 1056 1338
Size: 29 724 303 282
Fragments arranged by size:
724 303 282 29
BsaHI Gr'CG_yC
Cuts at: 0 21 188 848 929 1338
Size: 21 167 660 81 409
Fragments arranged by size:
660 409 167 81 21
BsaWI w'CCGG_w
Cuts at: 0 236 679 694 1338
Size: 236 443 15 644
Fragments arranged by size:
644 443 236 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 727 757 1338
Size: 727 30 581
Fragments arranged by size:
727 581 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 166 394 577 1338
Size: 148 18 228 183 761
Fragments arranged by size:
761 228 183 148 18
BseYI C'CCAG_C
Cuts at: 0 271 477 496 883 1338
Size: 271 206 19 387 455
Fragments arranged by size:
455 387 271 206 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1243 1338
Size: 1139 104 95
Fragments arranged by size:
1139 104 95
BsiEI CG_ry'CG
Cuts at: 0 108 684 1338
Size: 108 576 654
Fragments arranged by size:
654 576 108
BsiHKAI G_wGCw'C
Cuts at: 0 167 1232 1338
Size: 167 1065 106
Fragments arranged by size:
1065 167 106
Bsp1286I G_dGCh'C
Cuts at: 0 85 167 440 569 962 993 1232 1279
Size: 85 82 273 129 393 31 239 47
Cuts at: 1279 1286 1338
Size: 7 52
Fragments arranged by size:
393 273 239 129 85 82 52 47
31 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 543 806 1338
Size: 107 436 263 532
Fragments arranged by size:
532 436 263 107
BsrBI CCG'CTC
Cuts at: 0 408 1158 1338
Size: 408 750 180
Fragments arranged by size:
750 408 180
BsrFI r'CCGG_y
Cuts at: 0 679 694 1338
Size: 679 15 644
Fragments arranged by size:
679 644 15
BssHII G'CGCG_C
Cuts at: 0 7 1338
Size: 7 1331
BstAPI GCAn_nnn'nTGC
Cuts at: 0 330 893 1218 1338
Size: 330 563 325 120
Fragments arranged by size:
563 330 325 120
BstEII G'GTnAC_C
Cuts at: 0 675 806 1338
Size: 675 131 532
Fragments arranged by size:
675 532 131
BstXI CCAn_nnnn'nTGG
Cuts at: 0 448 1338
Size: 448 890
BstYI r'GATC_y
Cuts at: 0 998 1338
Size: 998 340
Bsu36I CC'TnA_GG
Cuts at: 0 1166 1338
Size: 1166 172
BtgI C'CryG_G
Cuts at: 0 47 431 1338
Size: 47 384 907
Fragments arranged by size:
907 384 47
BtsI GCAGTG_nn'
Cuts at: 0 369 808 1157 1279 1338
Size: 369 439 349 122 59
Fragments arranged by size:
439 369 349 122 59
DraIII CAC_nnn'GTG
Cuts at: 0 1139 1338
Size: 1139 199
DrdI GACnn_nn'nnGTC
Cuts at: 0 934 1099 1338
Size: 934 165 239
Fragments arranged by size:
934 239 165
EaeI y'GGCC_r
Cuts at: 0 105 428 763 1037 1199 1338
Size: 105 323 335 274 162 139
Fragments arranged by size:
335 323 274 162 139 105
EagI C'GGCC_G
Cuts at: 0 105 1338
Size: 105 1233
EarI CTCTTCn'nnn_
Cuts at: 0 371 386 398 479 1338
Size: 371 15 12 81 859
Fragments arranged by size:
859 371 81 15 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 966 1270 1338
Size: 442 524 304 68
Fragments arranged by size:
524 442 304 68
EcoO109I rG'GnC_Cy
Cuts at: 0 81 275 573 795 989 990 1005 1143
Size: 81 194 298 222 194 1 15 138
Cuts at: 1143 1161 1338
Size: 18 177
Fragments arranged by size:
298 222 194 194 177 138 81 18
15 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 636 1338
Size: 636 702
HaeII r_GCGC'y
Cuts at: 0 191 293 1303 1338
Size: 191 102 1010 35
Fragments arranged by size:
1010 191 102 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 312 344 727 759 1338
Size: 312 32 383 32 579
Fragments arranged by size:
579 383 312 32 32
KasI G'GCGC_C
Cuts at: 0 187 1338
Size: 187 1151
MscI TGG'CCA
Cuts at: 0 430 1039 1338
Size: 430 609 299
Fragments arranged by size:
609 430 299
MslI CAynn'nnrTG
Cuts at: 0 446 1338
Size: 446 892
MspA1I CmG'CkG
Cuts at: 0 420 762 844 944 1097 1218 1338
Size: 420 342 82 100 153 121 120
Fragments arranged by size:
420 342 153 121 120 100 82
NarI GG'CG_CC
Cuts at: 0 188 1338
Size: 188 1150
NcoI C'CATG_G
Cuts at: 0 47 431 1338
Size: 47 384 907
Fragments arranged by size:
907 384 47
NspI r_CATG'y
Cuts at: 0 900 1338
Size: 900 438
PasI CC'CwG_GG
Cuts at: 0 1279 1338
Size: 1279 59
PflMI CCAn_nnn'nTGG
Cuts at: 0 576 1338
Size: 576 762
PfoI T'CCnGG_A
Cuts at: 0 215 590 1338
Size: 215 375 748
Fragments arranged by size:
748 375 215
PmlI CAC'GTG
Cuts at: 0 29 753 1056 1338
Size: 29 724 303 282
Fragments arranged by size:
724 303 282 29
PpuMI rG'GwC_Cy
Cuts at: 0 275 795 1005 1143 1161 1338
Size: 275 520 210 138 18 177
Fragments arranged by size:
520 275 210 177 138 18
PshAI GACnn'nnGTC
Cuts at: 0 1072 1338
Size: 1072 266
PspOMI G'GGCC_C
Cuts at: 0 81 989 1338
Size: 81 908 349
Fragments arranged by size:
908 349 81
PstI C_TGCA'G
Cuts at: 0 554 803 1338
Size: 554 249 535
Fragments arranged by size:
554 535 249
PvuII CAG'CTG
Cuts at: 0 420 844 944 1218 1338
Size: 420 424 100 274 120
Fragments arranged by size:
424 420 274 120 100
SanDI GG'GwC_CC
Cuts at: 0 275 1161 1338
Size: 275 886 177
Fragments arranged by size:
886 275 177
SapI GCTCTTCn'nnn_
Cuts at: 0 398 479 1338
Size: 398 81 859
Fragments arranged by size:
859 398 81
SfcI C'TryA_G
Cuts at: 0 550 799 1338
Size: 550 249 539
Fragments arranged by size:
550 539 249
SfoI GGC'GCC
Cuts at: 0 189 1338
Size: 189 1149
SmaI CCC'GGG
Cuts at: 0 673 1338
Size: 673 665
SmlI C'TyrA_G
Cuts at: 0 994 1338
Size: 994 344
SphI G_CATG'C
Cuts at: 0 900 1338
Size: 900 438
StuI AGG'CCT
Cuts at: 0 157 892 1338
Size: 157 735 446
Fragments arranged by size:
735 446 157
StyI C'CwwG_G
Cuts at: 0 47 431 1338
Size: 47 384 907
Fragments arranged by size:
907 384 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 469 1258 1338
Size: 469 789 80
Fragments arranged by size:
789 469 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 787 819 1338
Size: 787 32 519
Fragments arranged by size:
787 519 32
Tth111I GACn'n_nGTC
Cuts at: 0 211 1338
Size: 211 1127
XmaI C'CCGG_G
Cuts at: 0 671 1338
Size: 671 667
ZraI GAC'GTC
Cuts at: 0 930 1338
Size: 930 408
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbvCI BcgI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EagI EarI Eco57MI EcoO109I FalI HaeII Hin4I
KasI MscI MslI MspA1I NarI NcoI NspI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII
SanDI SapI SfcI SfoI SmaI SmlI SphI StuI
StyI TaqII TstI Tth111I XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AhdI AlfI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI
BclI BfrBI BglI BmtI BplI BsaBI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstZ17I
BtgZI ClaI CspCI DraI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PpiI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI TatI XbaI XcmI XhoI XmnI