Restriction map for
pF1KSDA1949
(Linear) (Six-base) MAPSORT of: KIAA1949.seq Check: 7976 from: 1 to: 1839
KIAA1949.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 663 1839
Size: 663 1176
Acc65I G'GTAC_C
Cuts at: 0 1514 1839
Size: 1514 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1608 1839
Size: 1608 231
AflII C'TTAA_G
Cuts at: 0 1612 1839
Size: 1612 227
ApaI G_GGCC'C
Cuts at: 0 1392 1693 1839
Size: 1392 301 146
Fragments arranged by size:
1392 301 146
ApaLI G'TGCA_C
Cuts at: 0 253 1839
Size: 253 1586
AvaI C'yCGr_G
Cuts at: 0 356 506 626 1022 1494 1839
Size: 356 150 120 396 472 345
Fragments arranged by size:
472 396 356 345 150 120
BamHI G'GATC_C
Cuts at: 0 1335 1839
Size: 1335 504
BanI G'GyrC_C
Cuts at: 0 41 1514 1839
Size: 41 1473 325
Fragments arranged by size:
1473 325 41
BanII G_rGCy'C
Cuts at: 0 128 400 477 550 673 1155 1176 1268
Size: 128 272 77 73 123 482 21 92
Cuts at: 1268 1392 1693 1787 1839
Size: 124 301 94 52
Fragments arranged by size:
482 301 272 128 124 123 94 92
77 73 52 21
BbeI G_GCGC'C
Cuts at: 0 45 1839
Size: 45 1794
BbvCI CC'TCA_GC
Cuts at: 0 787 1839
Size: 787 1052
BglII A'GATC_T
Cuts at: 0 1532 1616 1839
Size: 1532 84 223
Fragments arranged by size:
1532 223 84
Bme1580I G_kGCm'C
Cuts at: 0 257 1199 1392 1442 1693 1716 1839
Size: 257 942 193 50 251 23 123
Fragments arranged by size:
942 257 251 193 123 50 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 1703 1839
Size: 1703 136
BmtI G_CTAG'C
Cuts at: 0 37 328 1839
Size: 37 291 1511
Fragments arranged by size:
1511 291 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 191 256 370 520 534 581 608
Size: 151 40 65 114 150 14 47 27
Cuts at: 608 717 787 1058 1139 1250 1252 1454 1660
Size: 109 70 271 81 111 2 202 206
Cuts at: 1660 1699 1709 1771 1839
Size: 39 10 62 68
Fragments arranged by size:
271 206 202 151 150 114 111 109
81 70 68 65 62 47 40 39
27 14 10 2
Bpu10I CC'TnA_GC
Cuts at: 0 787 1154 1839
Size: 787 367 685
Fragments arranged by size:
787 685 367
BsaI GGTCTCn'nnnn_
Cuts at: 0 360 436 717 883 1636 1839
Size: 360 76 281 166 753 203
Fragments arranged by size:
753 360 281 203 166 76
BsaAI yAC'GTr
Cuts at: 0 1649 1839
Size: 1649 190
BsaHI Gr'CG_yC
Cuts at: 0 42 58 1839
Size: 42 16 1781
Fragments arranged by size:
1781 42 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 43 73 82 110 112 140 532 562
Size: 43 30 9 28 2 28 392 30
Cuts at: 562 567 597 1633 1663 1839
Size: 5 30 1036 30 176
Fragments arranged by size:
1036 392 176 43 30 30 30 30
28 28 9 5 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 468 1156 1543 1696 1839
Size: 67 401 688 387 153 143
Fragments arranged by size:
688 401 387 153 143 67
BseYI C'CCAG_C
Cuts at: 0 370 459 1222 1305 1452 1501 1686 1839
Size: 370 89 763 83 147 49 185 153
Fragments arranged by size:
763 370 185 153 147 89 83 49
BsiHKAI G_wGCw'C
Cuts at: 0 257 550 1268 1787 1839
Size: 257 293 718 519 52
Fragments arranged by size:
718 519 293 257 52
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1564 1839
Size: 1564 275
Bsp1286I G_dGCh'C
Cuts at: 0 128 257 400 477 550 673 1155 1176
Size: 128 129 143 77 73 123 482 21
Cuts at: 1176 1199 1268 1392 1442 1693 1716 1787 1839
Size: 23 69 124 50 251 23 71 52
Fragments arranged by size:
482 251 143 129 128 124 123 77
73 71 69 52 50 23 23 21
BspHI T'CATG_A
Cuts at: 0 1343 1839
Size: 1343 496
BsrBI CCG'CTC
Cuts at: 0 282 363 603 1839
Size: 282 81 240 1236
Fragments arranged by size:
1236 282 240 81
BsrFI r'CCGG_y
Cuts at: 0 44 1832 1839
Size: 44 1788 7
Fragments arranged by size:
1788 44 7
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1330 1839
Size: 1330 509
BstYI r'GATC_y
Cuts at: 0 1335 1532 1616 1839
Size: 1335 197 84 223
Fragments arranged by size:
1335 223 197 84
Bsu36I CC'TnA_GG
Cuts at: 0 490 1700 1839
Size: 490 1210 139
Fragments arranged by size:
1210 490 139
BtsI GCAGTG_nn'
Cuts at: 0 1265 1407 1839
Size: 1265 142 432
Fragments arranged by size:
1265 432 142
EaeI y'GGCC_r
Cuts at: 0 2 844 1839
Size: 2 842 995
Fragments arranged by size:
995 842 2
EarI CTCTTCn'nnn_
Cuts at: 0 311 402 416 833 1244 1742 1754 1839
Size: 311 91 14 417 411 498 12 85
Fragments arranged by size:
498 417 411 311 91 85 14 12
EcoICRI GAG'CTC
Cuts at: 0 548 1266 1785 1839
Size: 548 718 519 54
Fragments arranged by size:
718 548 519 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 191 256 370 520 534 581 608
Size: 151 40 65 114 150 14 47 27
Cuts at: 608 717 787 1058 1139 1250 1252 1454 1608
Size: 109 70 271 81 111 2 202 154
Cuts at: 1608 1660 1699 1709 1771 1839
Size: 52 39 10 62 68
Fragments arranged by size:
271 202 154 151 150 114 111 109
81 70 68 65 62 52 47 40
39 27 14 10 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 182 235 706 1579 1839
Size: 182 53 471 873 260
Fragments arranged by size:
873 471 260 182 53
EcoO109I rG'GnC_Cy
Cuts at: 0 1388 1389 1586 1704 1809 1839
Size: 1388 1 197 118 105 30
Fragments arranged by size:
1388 197 118 105 30 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1638 1839
Size: 1638 201
FspI TGC'GCA
Cuts at: 0 1801 1839
Size: 1801 38
HaeII r_GCGC'y
Cuts at: 0 45 96 138 1839
Size: 45 51 42 1701
Fragments arranged by size:
1701 51 45 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 82 114 375 407 417 449
Size: 43 32 7 32 261 32 10 32
Cuts at: 449 532 564 567 599 1839
Size: 83 32 3 32 1240
Fragments arranged by size:
1240 261 83 43 32 32 32 32
32 32 10 7 3
HincII GTy'rAC
Cuts at: 0 1422 1839
Size: 1422 417
HindIII A'AGCT_T
Cuts at: 0 148 1090 1839
Size: 148 942 749
Fragments arranged by size:
942 749 148
KasI G'GCGC_C
Cuts at: 0 41 1839
Size: 41 1798
KpnI G_GTAC'C
Cuts at: 0 1518 1839
Size: 1518 321
MscI TGG'CCA
Cuts at: 0 4 846 1839
Size: 4 842 993
Fragments arranged by size:
993 842 4
MslI CAynn'nnrTG
Cuts at: 0 570 1839
Size: 570 1269
MspA1I CmG'CkG
Cuts at: 0 30 1571 1839
Size: 30 1541 268
Fragments arranged by size:
1541 268 30
NaeI GCC'GGC
Cuts at: 0 46 1834 1839
Size: 46 1788 5
Fragments arranged by size:
1788 46 5
NarI GG'CG_CC
Cuts at: 0 42 1839
Size: 42 1797
NgoMIV G'CCGG_C
Cuts at: 0 44 1832 1839
Size: 44 1788 7
Fragments arranged by size:
1788 44 7
NheI G'CTAG_C
Cuts at: 0 33 324 1839
Size: 33 291 1515
Fragments arranged by size:
1515 291 33
PasI CC'CwG_GG
Cuts at: 0 163 376 673 1037 1839
Size: 163 213 297 364 802
Fragments arranged by size:
802 364 297 213 163
PflMI CCAn_nnn'nTGG
Cuts at: 0 696 1839
Size: 696 1143
PfoI T'CCnGG_A
Cuts at: 0 128 585 1012 1839
Size: 128 457 427 827
Fragments arranged by size:
827 457 427 128
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 1839
Size: 1174 32 633
Fragments arranged by size:
1174 633 32
PpuMI rG'GwC_Cy
Cuts at: 0 1586 1704 1839
Size: 1586 118 135
Fragments arranged by size:
1586 135 118
PspOMI G'GGCC_C
Cuts at: 0 1388 1689 1839
Size: 1388 301 150
Fragments arranged by size:
1388 301 150
PstI C_TGCA'G
Cuts at: 0 879 1181 1491 1778 1839
Size: 879 302 310 287 61
Fragments arranged by size:
879 310 302 287 61
PvuII CAG'CTG
Cuts at: 0 30 1571 1839
Size: 30 1541 268
Fragments arranged by size:
1541 268 30
SacI G_AGCT'C
Cuts at: 0 550 1268 1787 1839
Size: 550 718 519 52
Fragments arranged by size:
718 550 519 52
SanDI GG'GwC_CC
Cuts at: 0 1586 1839
Size: 1586 253
SapI GCTCTTCn'nnn_
Cuts at: 0 311 1754 1839
Size: 311 1443 85
Fragments arranged by size:
1443 311 85
SfcI C'TryA_G
Cuts at: 0 25 191 875 1177 1487 1774 1839
Size: 25 166 684 302 310 287 65
Fragments arranged by size:
684 310 302 287 166 65 25
SfoI GGC'GCC
Cuts at: 0 43 1839
Size: 43 1796
SmaI CCC'GGG
Cuts at: 0 358 1496 1839
Size: 358 1138 343
Fragments arranged by size:
1138 358 343
SmlI C'TyrA_G
Cuts at: 0 626 1022 1612 1839
Size: 626 396 590 227
Fragments arranged by size:
626 590 396 227
StuI AGG'CCT
Cuts at: 0 495 951 1164 1235 1839
Size: 495 456 213 71 604
Fragments arranged by size:
604 495 456 213 71
StyI C'CwwG_G
Cuts at: 0 401 909 1580 1805 1839
Size: 401 508 671 225 34
Fragments arranged by size:
671 508 401 225 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1050 1839
Size: 1050 789
Tth111I GACn'n_nGTC
Cuts at: 0 431 1839
Size: 431 1408
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 989 1839
Size: 989 850
XhoI C'TCGA_G
Cuts at: 0 626 1022 1839
Size: 626 396 817
Fragments arranged by size:
817 626 396
XmaI C'CCGG_G
Cuts at: 0 356 1494 1839
Size: 356 1138 345
Fragments arranged by size:
1138 356 345
XmnI GAAnn'nnTTC
Cuts at: 0 265 1839
Size: 265 1574
Enzymes that do cut:
AccI Acc65I AcuI AflII ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbvCI BglII Bme1580I BmrI BmtI
BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrFI BstXI BstYI
Bsu36I BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MscI MslI MspA1I NaeI NarI NgoMIV NheI PasI
PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI
SanDI SapI SfcI SfoI SmaI SmlI StuI StyI
TaqII Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BplI BpuEI BsaBI BsaWI BsgI BsiEI BsiWI
BsmI BspEI BspMI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoRI EcoRV FseI FspAI
HpaI MfeI MluI MmeI NcoI NdeI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI XbaI ZraI